Efficient Large-Scale and Scarless Genome Engineering Enables the Construction and Screening of Bacillus subtilis Biofuel Overproducers

Bottom-up de novo design of functional proteins with complex structural features

(2021) Che Yang et al.   Nature Chemical Biology

Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1

(2021) Zeng‐xin Ma et al.   Biotechnology Journal

Bacillus subtilis as a robust host for biochemical production utilizing biomass

(2021) Seo A. Park et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Dynamics of Ku and bacterial non-homologous end-joining characterized using single DNA molecule analysis

(2021) Robin Öz et al.   NUCLEIC ACIDS RESEARCH

Engineering of Multiple Modules to Improve Amorphadiene Production in Bacillus subtilis Using CRISPR-Cas9

(2021) Yafeng Song et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

RecA Is Required for the Assembly of RecN into DNA Repair Complexes on the Nucleoid

(2021) Emma K. McLean et al.   JOURNAL OF BACTERIOLOGY

Chances and limitations when uncovering essential and non‐essential genes of Bacillus subtilis phages with CRISPR‐Cas9

(2021) Katharina Kohm et al.   Environmental Microbiology Reports

Systematic detection of functional proteoform groups from bottom-up proteomic datasets

(2021) Isabell Bludau et al.   Nature Communications

A Simplified Method for CRISPR-Cas9 Engineering of Bacillus subtilis

(2021) Ankita J. Sachla et al.   Microbiology Spectrum

The CRISPR toolbox for the gram-positive model bacterium Bacillus subtilis

(2021) Vitoria Fernanda Bertolazzi Zocca et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Top-down synthetic biology approach for titer improvement of clinically important antibiotic daptomycin in Streptomyces roseosporus

(2021) Chang-Hun Ji et al.   METABOLIC ENGINEERING

The Bacillus subtilis Minimal Genome Compendium

(2021) Stephan Michalik et al.   ACS Synthetic Biology

Microbial engineering for the production of isobutanol: current status and future directions

(2021) Nair M Lakshmi et al.   Bioengineered

Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering

(2020) Chaoyong Huang et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Positioning Bacillus subtilis as Terpenoid Cell Factory

(2020) Hegar Pramastya et al.   JOURNAL OF APPLIED MICROBIOLOGY

Bacillus subtilis cell diameter is determined by the opposing actions of two distinct cell wall synthetic systems

(2019) Michael F. Dion et al.   Nature Microbiology

MinGenome: An In Silico Top-Down Approach for the Synthesis of Minimized Genomes

(2018) Lin Wang et al.   ACS Synthetic Biology

Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis

(2018) Kun-Qiang Hong et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

(2018) Wenjing Cui et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Less Is More: Toward a Genome-Reduced Bacillus Cell Factory for “Difficult Proteins”

(2018) Rocío Aguilar Suárez et al.   ACS Synthetic Biology

A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

(2017) Younju So et al.   Frontiers in Microbiology

Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

(2017) Byoung-Mo Koo et al.   Cell Systems

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

(2016) Josef Altenbuchner   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Large-scale reduction of theBacillus subtilisgenome: consequences for the transcriptional network, resource allocation, and metabolism

(2016) Daniel R. Reuß et al.   GENOME RESEARCH

Characterization of genome-reduced Bacillus subtilis strains and their application for the production of guanosine and thymidine

(2016) Yang Li et al.   Microbial Cell Factories

The Next Generation of Synthetic Biology Chassis: Moving Synthetic Biology from the Laboratory to the Field

(2016) Bryn L. Adams   ACS Synthetic Biology

A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

(2016) Tianyuan Su et al.   Scientific Reports

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis

(2015) Jana Kumpfmüller et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development of a markerless gene deletion system for Bacillus subtilis based on the mannose phosphoenolpyruvate-dependent phosphotransferase system

(2015) Marian Wenzel et al.   MICROBIOLOGY-SGM

Bacillus subtilis as heterologous host for the secretory production of the non-ribosomal cyclodepsipeptide enniatin

(2014) Sophia Zobel et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Overexpression of a non-native deoxyxylulose-dependent vitamin B6 pathway in Bacillus subtilis for the production of pyridoxine

(2014) Fabian M. Commichau et al.   METABOLIC ENGINEERING

Consolidated conversion of protein waste into biofuels and ammonia using Bacillus subtilis

(2014) Kwon-Young Choi et al.   METABOLIC ENGINEERING

Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering

(2014) M. Juhas et al.   MICROBIOLOGY-SGM

Unravelling the structural and mechanistic basis of CRISPR–Cas systems

(2014) John van der Oost et al.   NATURE REVIEWS MICROBIOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Building the repertoire of dispensable chromosome regions in Bacillus subtilis entails major refinement of cognate large-scale metabolic model

(2012) Kosei Tanaka et al.   NUCLEIC ACIDS RESEARCH

Inhibitory cross-talk upon introduction of a new metabolic pathway into an existing metabolic network

(2012) J. Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction and the biosynthetic 2-ketoisovalerate precursor pathway overexpression

(2011) Shanshan Li et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Synthetic biology: applications come of age

(2010) Ahmad S. Khalil et al.   NATURE REVIEWS GENETICS

Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism

(2010) M. Komatsu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

How to make a minimal genome for synthetic minimal cell

(2010) Liu-yan Zhang et al.   Protein & Cell

Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites

(2010) Hong Gao et al.   Protein & Cell

Acetolactate Synthase from Bacillus subtilis Serves as a 2-Ketoisovalerate Decarboxylase for Isobutanol Biosynthesis in Escherichia coli

(2009) S. Atsumi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Minimal Escherichia coli Cell for the Most Efficient Production of Ethanol from Hexoses and Pentoses

(2008) C. T. Trinh et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Enhanced Recombinant Protein Productivity by Genome Reduction in Bacillus subtilis

(2008) T. Morimoto et al.   DNA RESEARCH

Selection and optimization of microbial hosts for biofuels production

(2008) Curt R. Fischer et al.   METABOLIC ENGINEERING

Control of the cell elongation–division cycle by shuttling of PBP1 protein in Bacillus subtilis

(2008) Dennis Claessen et al.   MOLECULAR MICROBIOLOGY

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

(2008) Shota Atsumi et al.   NATURE