Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis

Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production

(2018) Jie Zhang et al.   METABOLIC ENGINEERING

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

(2018) Zehua Bao et al.   NATURE BIOTECHNOLOGY

Development of an Efficient Genome Editing Tool in Bacillus licheniformis Using CRISPR-Cas9 Nickase

(2018) Kaifeng Li et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli

(2017) Liya Liang et al.   METABOLIC ENGINEERING

CRISPR-Cpf1 assisted genome editing of Corynebacterium glutamicum

(2017) Yu Jiang et al.   Nature Communications

A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

(2017) Younju So et al.   Frontiers in Microbiology

Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

(2016) Adam W. Westbrook et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

(2016) Josef Altenbuchner   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA

(2016) Takashi Yamano et al.   CELL

Structure and Engineering of Francisella novicida Cas9

(2016) Hisato Hirano et al.   CELL

Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications

(2016) Andriy Didovyk et al.   CURRENT OPINION IN BIOTECHNOLOGY

Rewiring Cas9 to Target New PAM Sequences

(2016) Virginijus Siksnys et al.   MOLECULAR CELL

The crystal structure of Cpf1 in complex with CRISPR RNA

(2016) De Dong et al.   NATURE

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system

(2016) Kang Zhang et al.   Scientific Reports

Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis

(2015) Jana Kumpfmüller et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Genome scale engineering techniques for metabolic engineering

(2015) Rongming Liu et al.   METABOLIC ENGINEERING

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Bacillus subtilis as heterologous host for the secretory production of the non-ribosomal cyclodepsipeptide enniatin

(2014) Sophia Zobel et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Rational design of highly active sgRNAs for CRISPR-Cas9–mediated gene inactivation

(2014) John G Doench et al.   NATURE BIOTECHNOLOGY

Unravelling the structural and mechanistic basis of CRISPR–Cas systems

(2014) John van der Oost et al.   NATURE REVIEWS MICROBIOLOGY

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

CRISPR–Cas9-assisted recombineering in Lactobacillus reuteri

(2014) Jee-Hwan Oh et al.   NUCLEIC ACIDS RESEARCH

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Generation of gene-modified mice via Cas9/RNA-mediated gene targeting

(2013) Bin Shen et al.   CELL RESEARCH

The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori

(2013) Yueqiang Wang et al.   CELL RESEARCH

Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila

(2013) Z. Yu et al.   GENETICS

Bacillus subtilis: from soil bacterium to super-secreting cell factory

(2013) Jan Maarten van Dijl et al.   Microbial Cell Factories

Zinc Fingers, TAL Effectors, or Cas9-Based DNA Binding Proteins: What’s Best for Targeting Desired Genome Loci?

(2013) Annett Strauβ et al.   Molecular Plant

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome

(2013) Ting Shi et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Cytotoxic Chromosomal Targeting by CRISPR/Cas Systems Can Reshape Bacterial Genomes and Expel or Remodel Pathogenicity Islands

(2013) Reuben B. Vercoe et al.   PLoS Genetics

A Guide RNA Sequence Design Platform for the CRISPR/Cas9 System for Model Organism Genomes

(2013) Ming Ma et al.   Biomed Research International

Essential Features and Rational Design of CRISPR RNAs that Function with the Cas RAMP Module Complex to Cleave RNAs

(2012) Caryn R. Hale et al.   MOLECULAR CELL

Fast gapped-read alignment with Bowtie 2

(2012) Ben Langmead et al.   NATURE METHODS

TALENs: a widely applicable technology for targeted genome editing

(2012) J. Keith Joung et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria

(2012) G. Gasiunas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Genome Engineering With Zinc-Finger Nucleases

(2011) Dana Carroll   GENETICS

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Construction of Chromosomally Located T7 Expression System for Production of Heterologous Secreted Proteins in Bacillus subtilis

(2010) Po Ting Chen et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ZiFiT (Zinc Finger Targeter): an updated zinc finger engineering tool

(2010) J. D. Sander et al.   NUCLEIC ACIDS RESEARCH

Self-targeting by CRISPR: gene regulation or autoimmunity?

(2010) Adi Stern et al.   TRENDS IN GENETICS

Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis

(2008) X. Yan et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY