Engineering of Multiple Modules to Improve Amorphadiene Production in Bacillus subtilis Using CRISPR-Cas9

A regulated synthetic operon facilitates stable overexpression of multigene terpenoid pathway in Bacillus subtilis

(2020) Ingy I. Abdallah et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Advances in the biosynthesis, regulation, and metabolic engineering of plant specialized terpenoids

(2020) Dinesh A. Nagegowda et al.   PLANT SCIENCE

Production of Squalene in Bacillus subtilis by Squalene Synthase Screening and Metabolic Engineering

(2020) Yafeng Song et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis

(2020) Sili Yu et al.   ACS Synthetic Biology

Positioning Bacillus subtilis as Terpenoid Cell Factory

(2020) Hegar Pramastya et al.   JOURNAL OF APPLIED MICROBIOLOGY

High Level Production of Amorphadiene using Bacillus subtilis as an Optimized Terpenoid Cell Factory

(2020) Hegar Pramastya et al.   New Biotechnology

CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis

(2019) Marcus A. Price et al.   PLoS One

Metabolic Engineering of Bacillus subtilis Toward Taxadiene Biosynthesis as the First Committed Step for Taxol Production

(2019) Ingy I. Abdallah et al.   Frontiers in Microbiology

Engineering a Bifunctional Phr60-Rap60-Spo0A Quorum-Sensing Molecular Switch for Dynamic Fine-Tuning of Menaquinone-7 Synthesis in Bacillus subtilis

(2019) Shixiu Cui et al.   ACS Synthetic Biology

Metabolic Engineering of the MEP Pathway in Bacillus subtilis for Increased Biosynthesis of Menaquinone-7

(2019) Yanwei Ma et al.   ACS Synthetic Biology

CRISPR-Cas9 system: A new-fangled dawn in gene editing

(2019) Darshana Gupta et al.   LIFE SCIENCES

Systematic engineering for high-yield production of viridiflorol and amorphadiene in auxotrophic Escherichia coli

(2019) Sudha Shukal et al.   METABOLIC ENGINEERING

Combinatorial modulation of initial codons for improved zeaxanthin synthetic pathway efficiency in Escherichia coli

(2019) Zaiqiang Wu et al.   MicrobiologyOpen

Catalysis of amorpha-4,11-diene synthase unraveled and improved by mutability landscape guided engineering

(2018) Ingy I. Abdallah et al.   Scientific Reports

In-depth analysis of Bacillus subtilis proteome identifies new ORFs and traces the evolutionary history of modified proteins

(2018) Vaishnavi Ravikumar et al.   Scientific Reports

Modular Pathway Engineering of Bacillus subtilis To Promote De Novo Biosynthesis of Menaquinone-7

(2018) Shaomei Yang et al.   ACS Synthetic Biology

Rewiring the Glucose Transportation and Central Metabolic Pathways for Overproduction of N -Acetylglucosamine in Bacillus subtilis

(2017) Yang Gu et al.   Biotechnology Journal

A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

(2017) Younju So et al.   Frontiers in Microbiology

Boosting isoprene production via heterologous expression of the Kudzu isoprene synthase gene (kIspS) into Bacillus spp. cell factory

(2017) Lamis Gomaa et al.   AMB Express

Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

(2017) Byoung-Mo Koo et al.   Cell Systems

Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

(2016) Adam W. Westbrook et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

(2016) Josef Altenbuchner   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Depletion of Undecaprenyl Pyrophosphate Phosphatases Disrupts Cell Envelope Biogenesis in Bacillus subtilis

(2016) Heng Zhao et al.   JOURNAL OF BACTERIOLOGY

Enhanced C30 carotenoid production in Bacillus subtilis by systematic overexpression of MEP pathway genes

(2015) Dan Xue et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Dead-end intermediates in the enterobacterial common antigen pathway induce morphological defects inEscherichia coliby competing for undecaprenyl phosphate

(2015) Matthew A. Jorgenson et al.   MOLECULAR MICROBIOLOGY

Current development in genetic engineering strategies of Bacillus species

(2014) Huina Dong et al.   Microbial Cell Factories

A time resolved metabolomics study: the influence of different carbon sources during growth and starvation of Bacillus subtilis

(2014) Hanna Meyer et al.   Molecular BioSystems

Model-Driven Redox Pathway Manipulation for Improved Isobutanol Production in Bacillus subtilis Complemented with Experimental Validation and Metabolic Profiling Analysis

(2014) Haishan Qi et al.   PLoS One

Rational engineering of plasticity residues of sesquiterpene synthases fromArtemisia annua: product specificity and catalytic efficiency

(2013) Jian-Xu Li et al.   BIOCHEMICAL JOURNAL

Optimization of amorphadiene synthesis inbacillus subtilisvia transcriptional, translational, and media modulation

(2013) Kang Zhou et al.   BIOTECHNOLOGY AND BIOENGINEERING

Complex formation between malate dehydrogenase and isocitrate dehydrogenase fromBacillus subtilisis regulated by tricarboxylic acid cycle metabolites

(2013) Maike Bartholomae et al.   FEBS Journal

Engineering central metabolic modules of Escherichia coli for improving β-carotene production

(2013) Jing Zhao et al.   METABOLIC ENGINEERING

High-level semi-synthetic production of the potent antimalarial artemisinin

(2013) C. J. Paddon et al.   NATURE

Coregulation of Terpenoid Pathway Genes and Prediction of Isoprene Production in Bacillus subtilis Using Transcriptomics

(2013) Becky M. Hess et al.   PLoS One

Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis

(2013) Gidi Pelchovich et al.   PLoS One

The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis

(2013) Jara Radeck et al.   Journal of Biological Engineering

Undecaprenyl Pyrophosphate Involvement in Susceptibility of Bacillus subtilis to Rare Earth Elements

(2012) T. Inaoka et al.   JOURNAL OF BACTERIOLOGY

Production of amorphadiene in yeast, and its conversion to dihydroartemisinic acid, precursor to the antimalarial agent artemisinin

(2012) P. J. Westfall et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Condition-Dependent Transcriptome Reveals High-Level Regulatory Architecture in Bacillus subtilis

(2012) P. Nicolas et al.   SCIENCE

Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

(2011) Chun You et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in Escherichia coli

(2010) P. K. Ajikumar et al.   SCIENCE

Carotenoid production in Bacillus subtilis achieved by metabolic engineering

(2009) Kazuyuki Yoshida et al.   BIOTECHNOLOGY LETTERS

Terpenoid biomaterials

(2008) Jörg Bohlmann et al.   PLANT JOURNAL