Structure of the RZZ complex and molecular basis of Spindly‐driven corona assembly at human kinetochores

The Disordered Spindly C-terminus Interacts with RZZ Subunits ROD-1 and ZWL-1 in the Kinetochore through the Same Sites in C. Elegans

(2021) Morkos A. Henen et al.   JOURNAL OF MOLECULAR BIOLOGY

Kinetochore assembly throughout the cell cycle

(2021) Alexandra P. Navarro et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Highly accurate protein structure prediction with AlphaFold

(2021) John Jumper et al.   NATURE

Highly accurate protein structure prediction for the human proteome

(2021) Kathryn Tunyasuvunakool et al.   NATURE

Endocytosis in the context-dependent regulation of individual and collective cell properties

(2021) Sara Sigismund et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Spindle assembly checkpoint activation and silencing at kinetochores

(2021) Pablo Lara-Gonzalez et al.   SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY

Cyclin B1 scaffolds MAD 1 at the kinetochore corona to activate the mitotic checkpoint

(2020) Lindsey A Allan et al.   EMBO JOURNAL

CENP-F stabilizes kinetochore-microtubule attachments and limits dynein stripping of corona cargoes

(2020) Philip Auckland et al.   JOURNAL OF CELL BIOLOGY

Cyclin B1-Cdk1 facilitates MAD1 release from the nuclear pore to ensure a robust spindle checkpoint

(2020) Mark Jackman et al.   JOURNAL OF CELL BIOLOGY

Crowning the Kinetochore: The Fibrous Corona in Chromosome Segregation

(2020) Geert J.P.L. Kops et al.   TRENDS IN CELL BIOLOGY

The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment

(2020) Thibault Legal et al.   JOURNAL OF CELL SCIENCE

A Biosensor for the Mitotic Kinase MPS1 Reveals Spatiotemporal Activity Dynamics and Regulation

(2020) Timo E.F. Kuijt et al.   CURRENT BIOLOGY

Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction

(2020) Ali Punjani et al.   NATURE METHODS

Electroporated recombinant proteins as tools for in vivo functional complementation, imaging and chemical biology

(2019) Amal Alex et al.   eLife

Namdinator – automatic molecular dynamics flexible fitting of structural models into cryo-EM and crystallography experimental maps

(2019) Rune Thomas Kidmose et al.   IUCrJ

Mammalian kinetochores count attached microtubules in a sensitive and switch-like manner

(2019) Jonathan Kuhn et al.   JOURNAL OF CELL BIOLOGY

Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix

(2019) Dorothee Liebschner et al.   Acta Crystallographica Section D-Structural Biology

Polo regulates Spindly to prevent premature stabilization of kinetochore–microtubule attachments

(2019) João Barbosa et al.   EMBO JOURNAL

The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases

(2018) Giuseppe Ciossani et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Correction: Formation of COPI-coated vesicles at a glance (doi:10.1242/jcs.209890)

(2018) Eric C. Arakel et al.   JOURNAL OF CELL SCIENCE

Dynamic kinetochore size regulation promotes microtubule capture and chromosome biorientation in mitosis

(2018) Carlos Sacristan et al.   NATURE CELL BIOLOGY

The cytoplasmic dynein transport machinery and its many cargoes

(2018) Samara L. Reck-Peterson et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

BUB1 Is Essential for the Viability of Human Cells in which the Spindle Assembly Checkpoint Is Compromised

(2018) Jonne A. Raaijmakers et al.   Cell Reports

Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion

(2018) Jose-Antonio Rodriguez-Rodriguez et al.   CURRENT BIOLOGY

Self-Assembly of the RZZ Complex into Filaments Drives Kinetochore Expansion in the Absence of Microtubule Attachment

(2018) Cláudia Pereira et al.   CURRENT BIOLOGY

A Bayesian approach to beam-induced motion correction in cryo-EM single-particle analysis

(2018) Jasenko Zivanov et al.   IUCrJ

Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division

(2018) Edmond R. Watson et al.   TRENDS IN CELL BIOLOGY

Molecular mechanism of dynein recruitment to kinetochores by the Rod–Zw10–Zwilch complex and Spindly

(2017) José B. Gama et al.   JOURNAL OF CELL BIOLOGY

Structure of the RZZ complex and molecular basis of its interaction with Spindly

(2017) Shyamal Mosalaganti et al.   JOURNAL OF CELL BIOLOGY

Spindle assembly checkpoint satisfaction occurs via end-on but not lateral attachments under tension

(2017) Jonathan Kuhn et al.   JOURNAL OF CELL BIOLOGY

Basis of catalytic assembly of the mitotic checkpoint complex

(2017) Alex C. Faesen et al.   NATURE

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C)

(2017) Claudio Alfieri et al.   Open Biology

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bicaudal D Family of Motor Adaptors: Linking Dynein Motility to Cargo Binding

(2016) Casper C. Hoogenraad et al.   TRENDS IN CELL BIOLOGY

Mechanism and Regulation of Cytoplasmic Dynein

(2015) Michael A. Cianfrocco et al.   Annual Review of Cell and Developmental Biology

KNL1-Bubs and RZZ Provide Two Separable Pathways for Checkpoint Activation at Human Kinetochores

(2015) Virginia Silió et al.   DEVELOPMENTAL CELL

Kinetochore function is controlled by a phospho-dependent coexpansion of inner and outer components

(2015) David J. Wynne et al.   JOURNAL OF CELL BIOLOGY

A novel role of farnesylation in targeting a mitotic checkpoint protein, human Spindly, to kinetochores

(2015) Devinderjit K. Moudgil et al.   JOURNAL OF CELL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Preventing farnesylation of the dynein adaptor Spindly contributes to the mitotic defects caused by farnesyltransferase inhibitors

(2015) Andrew J. Holland et al.   MOLECULAR BIOLOGY OF THE CELL

Adaptive changes in the kinetochore architecture facilitate proper spindle assembly

(2015) Valentin Magidson et al.   NATURE CELL BIOLOGY

Distinct domains in Bub1 localize RZZ and BubR1 to kinetochores to regulate the checkpoint

(2015) Gang Zhang et al.   Nature Communications

The RZZ complex requires the N-terminus of KNL1 to mediate optimal Mad1 kinetochore localization in human cells

(2015) Gina V. Caldas et al.   Open Biology

Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod–Zwilch–ZW10 (RZZ) complex

(2015) Anika Altenfeld et al.   Acta Crystallographica Section F-Structural Biology Communications

In vitroreconstitution of a highly processive recombinant human dynein complex

(2014) Max A Schlager et al.   EMBO JOURNAL

CENP-I and Aurora B act as a molecular switch that ties RZZ/Mad1 recruitment to kinetochore attachment status

(2014) Daniel R. Matson et al.   JOURNAL OF CELL BIOLOGY

Activation of cytoplasmic dynein motility by dynactin-cargo adapter complexes

(2014) R. J. McKenney et al.   SCIENCE

Bulk-solvent and overall scaling revisited: faster calculations, improved results

(2013) P. V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Spindle assembly checkpoint proteins are positioned close to core microtubule attachment sites at kinetochores

(2013) Dileep Varma et al.   JOURNAL OF CELL BIOLOGY

A TPR domain–containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B

(2013) Wilco Nijenhuis et al.   JOURNAL OF CELL BIOLOGY

Systematic dissection of dynein regulators in mitosis

(2013) Jonne A. Raaijmakers et al.   JOURNAL OF CELL BIOLOGY

Crosstalk Between Microtubule Attachment Complexes Ensures Accurate Chromosome Segregation

(2013) D. K. Cheerambathur et al.   SCIENCE

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Iterative Stable Alignment and Clustering of 2D Transmission Electron Microscope Images

(2012) Zhengfan Yang et al.   STRUCTURE

Probing thein vivofunction of Mad1:C-Mad2 in the spindle assembly checkpoint

(2011) Luca L Fava et al.   EMBO JOURNAL

The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore–microtubule attachment in mitosis

(2011) Lynsie J.R. Sundin et al.   MOLECULAR BIOLOGY OF THE CELL

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Structure of Coatomer Cage Proteins and the Relationship among COPI, COPII, and Clathrin Vesicle Coats

(2010) Changwook Lee et al.   CELL

Removal of Spindly from microtubule-attached kinetochores controls spindle checkpoint silencing in human cells

(2010) R. Gassmann et al.   GENES & DEVELOPMENT

Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine

(2010) Stefano Santaguida et al.   JOURNAL OF CELL BIOLOGY

Spindly/CCDC99 Is Required for Efficient Chromosome Congression and Mitotic Checkpoint Regulation

(2010) Marin Barisic et al.   MOLECULAR BIOLOGY OF THE CELL

Structural Analysis of the RZZ Complex Reveals Common Ancestry with Multisubunit Vesicle Tethering Machinery

(2010) Filiz Çivril et al.   STRUCTURE

Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint

(2009) Mylavarapu V S Sivaram et al.   EMBO JOURNAL

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly

(2009) Ying Wai Chan et al.   JOURNAL OF CELL BIOLOGY

Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex

(2009) Arati Tripathi et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Roles for the Conserved Spc105p/Kre28p Complex in Kinetochore-Microtubule Binding and the Spindle Assembly Checkpoint

(2009) Cinzia Pagliuca et al.   PLoS One

The Influence of Catalysis on Mad2 Activation Dynamics

(2009) Marco Simonetta et al.   PLOS BIOLOGY

Kinetochore Attachments Require an Interaction between Unstructured Tails on Microtubules and Ndc80Hec1

(2008) Stephanie A. Miller et al.   CURRENT BIOLOGY

A new mechanism controlling kinetochore-microtubule interactions revealed by comparison of two dynein-targeting components: SPDL-1 and the Rod/Zwilch/Zw10 complex

(2008) R. Gassmann et al.   GENES & DEVELOPMENT

Direct role of dynein motor in stable kinetochore-microtubule attachment, orientation, and alignment

(2008) Dileep Varma et al.   JOURNAL OF CELL BIOLOGY

SPDL-1 functions as a kinetochore receptor for MDF-1 inCaenorhabditis elegans

(2008) Takaharu G. Yamamoto et al.   JOURNAL OF CELL BIOLOGY

Dynein Light Intermediate Chain: An Essential Subunit That Contributes to Spindle Checkpoint Inactivation

(2008) Sarah Mische et al.   MOLECULAR BIOLOGY OF THE CELL

Structural Evidence for Common Ancestry of the Nuclear Pore Complex and Vesicle Coats

(2008) S. G. Brohawn et al.   SCIENCE