Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

CRISPR technologies and the search for the PAM-free nuclease

(2021) Daphne Collias et al.   Nature Communications

Efficient, Rapid, and Sensitive Detection of Plant RNA Viruses With One-Pot RT-RPA–CRISPR/Cas12a Assay

(2021) Rashid Aman et al.   Frontiers in Microbiology

LAMP-Coupled CRISPR–Cas12a Module for Rapid and Sensitive Detection of Plant DNA Viruses

(2021) Ahmed Mahas et al.   Viruses-Basel

Applications, challenges, and needs for employing synthetic biology beyond the lab

(2021) Sierra M. Brooks et al.   Nature Communications

Detect and destroy: CRISPR-based technologies for the response against viruses

(2021) Catherine A. Freije et al.   Cell Host & Microbe

CRISPR-based diagnostics

(2021) Michael M. Kaminski et al.   Nature Biomedical Engineering

The complex formed between a synthetic RNA aptamer and the transcription repressor TetR is a structural and functional twin of the operator DNA–TetR regulator complex

(2020) Florian C Grau et al.   NUCLEIC ACIDS RESEARCH

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies

(2020) Rashid Aman et al.   ACS Synthetic Biology

Advances in Cell‐Free Biosensors: Principle, Mechanism, and Applications

(2020) Liyuan Zhang et al.   Biotechnology Journal

Cell-free biosensors for rapid detection of water contaminants

(2020) Jaeyoung K. Jung et al.   NATURE BIOTECHNOLOGY

SPRINT: a Cas13a-based platform for detection of small molecules

(2020) Roman S Iwasaki et al.   NUCLEIC ACIDS RESEARCH

Ultrasensitive CRISPR-based diagnostic for field-applicable detection ofPlasmodiumspecies in symptomatic and asymptomatic malaria

(2020) Rose A. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-pass transcription by T7 RNA polymerase

(2020) Luiz F. M. Passalacqua et al.   RNA

iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

(2020) Zahir Ali et al.   VIRUS RESEARCH

Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals

(2019) Xinyi Wan et al.   Nature Chemical Biology

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Cell-free gene expression: an expanded repertoire of applications

(2019) Adam D. Silverman et al.   NATURE REVIEWS GENETICS

A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

(2019) Mindong Liang et al.   Nature Communications

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

(2019) Ying Xiong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

CRISPR/Cas13 as a Tool for RNA Interference

(2018) Zahir Ali et al.   TRENDS IN PLANT SCIENCE

Engineering virus resistance via CRISPR–Cas systems

(2018) Ahmed Mahas et al.   Current Opinion in Virology

Engineering RNA Virus Interference via the CRISPR/Cas13 Machinery in Arabidopsis

(2018) Rashid Aman et al.   Viruses-Basel

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

A Cell-Free Biosensor for Detecting Quorum Sensing Molecules in P. aeruginosa-Infected Respiratory Samples

(2017) Ke Yan Wen et al.   ACS Synthetic Biology

The CRISPR-associated DNA-cleaving enzyme Cpf1 also processes precursor CRISPR RNA

(2016) Ines Fonfara et al.   NATURE

Multiplex gene editing by CRISPR–Cpf1 using a single crRNA array

(2016) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Synthetic biology devices for in vitro and in vivo diagnostics

(2015) Shimyn Slomovic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection of pathological biomarkers in human clinical samples via amplifying genetic switches and logic gates

(2015) Alexis Courbet et al.   Science Translational Medicine

Regulatable and Modulable Background Expression Control in Prokaryotic Synthetic Circuits by Auxiliary Repressor Binding Sites

(2015) Davide Merulla et al.   ACS Synthetic Biology

Transcription factor-based biosensors enlightened by the analyte

(2015) Raul Fernandez-López et al.   Frontiers in Microbiology

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

(2014) Rodolphe Barrangou et al.   MOLECULAR CELL

Unravelling the structural and mechanistic basis of CRISPR–Cas systems

(2014) John van der Oost et al.   NATURE REVIEWS MICROBIOLOGY

The TetR Family of Regulators

(2013) L. Cuthbertson et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

RNA-guided genetic silencing systems in bacteria and archaea

(2012) Blake Wiedenheft et al.   NATURE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Mechanistic Basis for RNA Aptamer-Based Induction of TetR

(2011) Markus Steber et al.   CHEMBIOCHEM

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Synthetic biology: applications come of age

(2010) Ahmad S. Khalil et al.   NATURE REVIEWS GENETICS

Where microbiology meets microengineering: design and applications of reporter bacteria

(2010) Jan Roelof van der Meer et al.   NATURE REVIEWS MICROBIOLOGY