Detect and destroy: CRISPR-based technologies for the response against viruses

Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents

(2021) Emmeline L. Blanchard et al.   NATURE BIOTECHNOLOGY

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

(2020) Xusheng Wang et al.   ACS Nano

Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

(2020) Chaoqun Yuan et al.   ANALYTICAL CHEMISTRY

CRISPR-Cas13a Cleavage of Dengue Virus NS3 Gene Efficiently Inhibits Viral Replication

(2020) Hao Li et al.   Molecular Therapy-Nucleic Acids

Ultra-sensitive and high-throughput CRISPR-Powered COVID-19 diagnosis

(2020) Zhen Huang et al.   BIOSENSORS & BIOELECTRONICS

Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza

(2020) Timothy R. Abbott et al.   CELL

A SARS-CoV-2 protein interaction map reveals targets for drug repurposing

(2020) David E. Gordon et al.   NATURE

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

Massively parallel Cas13 screens reveal principles for guide RNA design

(2020) Hans-Hermann Wessels et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

(2020) Shuhan Lu et al.   Cell Discovery

SARS-CoV-2 detection with CRISPR diagnostics

(2020) Lu Guo et al.   Cell Discovery

Cas13b-dependent and Cas13b-independent RNA knockdown of viral sequences in mosquito cells following guide RNA expression

(2020) Priscilla Ying Lei Tng et al.   Communications Biology

SARS-CoV-2 pandemic: a review of molecular diagnostic tools including sample collection and commercial response with associated advantages and limitations

(2020) Harikrishnan Jayamohan et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

CRISPR/cas systems redefine nucleic acid detection: Principles and methods

(2020) Meng Wang et al.   BIOSENSORS & BIOELECTRONICS

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms

(2020) David E. Gordon et al.   SCIENCE

iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2

(2020) Zahir Ali et al.   VIRUS RESEARCH

Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time

(2020) Kayla G. Barnes et al.   Nature Communications

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

(2020) Tieying Hou et al.   PLoS Pathogens

CRISPR-Cas13a Inhibits HIV-1 Infection

(2020) Lijuan Yin et al.   Molecular Therapy-Nucleic Acids

Powassan Virus Neuropathology and Genomic Diversity in Patients With Fatal Encephalitis

(2020) Erica Normandin et al.   Open Forum Infectious Diseases

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection

(2020) Yanju Chen et al.   BIOSENSORS & BIOELECTRONICS

Amplification-free detection of SARS-CoV-2 with CRISPR-Cas13a and mobile phone microscopy

(2020) Parinaz Fozouni et al.   CELL

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Rapid and Fully Microfluidic Ebola Virus Detection with CRISPR-Cas13a

(2019) Peiwu Qin et al.   ACS Sensors

Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing

(2019) Benjamin P. Kleinstiver et al.   NATURE BIOTECHNOLOGY

HIV-1 remission following CCR5Δ32/Δ32 haematopoietic stem-cell transplantation

(2019) Ravindra K. Gupta et al.   NATURE

Taking connected mobile-health diagnostics of infectious diseases to the field

(2019) Christopher S. Wood et al.   NATURE

CRISPR-Cas13a Nanomachine Based Simple Technology for Avian Influenza A (H7N9) Virus On-Site Detection

(2019) Yufeng Liu et al.   Journal of Biomedical Nanotechnology

Room Temperature Detection of Plasma Epstein–Barr Virus DNA with CRISPR–Cas13

(2019) Yetao Wu et al.   CLINICAL CHEMISTRY

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Viral networks and detection of potential zoonotic viruses in bats and rodents: A worldwide analysis

(2019) Fabiola Nieto-Rabiela et al.   Zoonoses and Public Health

Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice

(2019) Prasanta K. Dash et al.   Nature Communications

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

CRISPR-Edited Stem Cells in a Patient with HIV and Acute Lymphocytic Leukemia

(2019) Lei Xu et al.   NEW ENGLAND JOURNAL OF MEDICINE

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

The CRISPR‐Cas13a Gene‐Editing System Induces Collateral Cleavage of RNA in Glioma Cells

(2019) Qixue Wang et al.   Advanced Science

Rational designs of in vivo CRISPR-Cas delivery systems

(2019) Cong-Fei Xu et al.   ADVANCED DRUG DELIVERY REVIEWS

Immunogenicity of Cas9 Protein

(2019) Aditi Mehta et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Engineered amphiphilic peptides enable delivery of proteins and CRISPR-associated nucleases to airway epithelia

(2019) Sateesh Krishnamurthy et al.   Nature Communications

Programmable Inhibition and Detection of RNA Viruses Using Cas13

(2019) Catherine A. Freije et al.   MOLECULAR CELL

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Detection of target DNA with a novel Cas9/sgRNAs-associated reverse PCR (CARP) technique

(2018) Beibei Zhang et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

The Biology of CRISPR-Cas: Backward and Forward

(2018) Frank Hille et al.   CELL

Simultaneous Knockout of CXCR4 and CCR5 Genes in CD4+ T Cells via CRISPR/Cas9 Confers Resistance to Both X4- and R5-Tropic Human Immunodeficiency Virus Type 1 Infection

(2018) Songlin Yu et al.   HUMAN GENE THERAPY

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR/Cas9 system targeting regulatory genes of HIV-1 inhibits viral replication in infected T-cell cultures

(2018) Youdiil Ophinni et al.   Scientific Reports

Removal of HIV DNA by CRISPR from Patient Blood Engrafts in Humanized Mice

(2018) Ramona Bella et al.   Molecular Therapy-Nucleic Acids

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Detecting and typing target DNA with a novel CRISPR-typing PCR (ctPCR) technique

(2018) Beibei Zhang et al.   ANALYTICAL BIOCHEMISTRY

HIV-1 Employs Multiple Mechanisms To Resist Cas9/Single Guide RNA Targeting the Viral Primer Binding Site

(2018) Zhen Wang et al.   JOURNAL OF VIROLOGY

RNA Guide Complementarity Prevents Self-Targeting in Type VI CRISPR Systems

(2018) Alexander J. Meeske et al.   MOLECULAR CELL

CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method

(2018) Qiao Wang et al.   Scientific Reports

Genome-edited baby claim provokes international outcry

(2018) David Cyranoski et al.   NATURE

High-throughput in vivo screen of functional mRNA delivery identifies nanoparticles for endothelial cell gene editing

(2018) Cory D. Sago et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programmed DNA destruction by miniature CRISPR-Cas14 enzymes

(2018) Lucas B. Harrington et al.   SCIENCE

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

(2017) Alexandra East-Seletsky et al.   MOLECULAR CELL

CRISPR/Cas9-Mediated CCR5 Ablation in Human Hematopoietic Stem/Progenitor Cells Confers HIV-1 Resistance In Vivo

(2017) Lei Xu et al.   MOLECULAR THERAPY

In Vivo Excision of HIV-1 Provirus by saCas9 and Multiplex Single-Guide RNAs in Animal Models

(2017) Chaoran Yin et al.   MOLECULAR THERAPY

Host and viral traits predict zoonotic spillover from mammals

(2017) Kevin J. Olival et al.   NATURE

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

Engineered Cpf1 variants with altered PAM specificities

(2017) Linyi Gao et al.   NATURE BIOTECHNOLOGY

Colorimetric tests for diagnosis of filarial infection and vector surveillance using non-instrumented nucleic acid loop-mediated isothermal amplification (NINA-LAMP)

(2017) Catherine B. Poole et al.   PLoS One

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Development and evaluation of a rapid molecular diagnostic test for Zika virus infection by reverse transcription loop-mediated isothermal amplification

(2017) Yohei Kurosaki et al.   Scientific Reports

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Approved Antiviral Drugs over the Past 50 Years

(2016) Erik De Clercq et al.   CLINICAL MICROBIOLOGY REVIEWS

An Effective Molecular Target Site in Hepatitis B Virus S Gene for Cas9 Cleavage and Mutational Inactivation

(2016) Hao Li et al.   International Journal of Biological Sciences

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus

(2016) Pranav Patel et al.   PLoS Neglected Tropical Diseases

CRISPR/Cas9-Mediated Genome Editing of Herpesviruses Limits Productive and Latent Infections

(2016) Ferdy R. van Diemen et al.   PLoS Pathogens

Inhibition of HSV-1 Replication by Gene Editing Strategy

(2016) Pamela C. Roehm et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Harnessing the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus

(2015) S Zhen et al.   GENE THERAPY

Inhibition of hepatitis B virus by the CRISPR/Cas9 system via targeting the conserved regions of the viral genome

(2015) Xing Liu et al.   JOURNAL OF GENERAL VIROLOGY

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Cas9-mediated targeting of viral RNA in eukaryotic cells

(2015) Aryn A. Price et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The CRISPR/Cas9 system inactivates latent HIV-1 proviral DNA

(2015) Weijun Zhu et al.   Retrovirology

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Dual gRNAs guided CRISPR/Cas9 system inhibits hepatitis B virus replication

(2015) Jie Wang   WORLD JOURNAL OF GASTROENTEROLOGY

CRISPR/Cas9 cleavage of viral DNA efficiently suppresses hepatitis B virus

(2015) Vyas Ramanan et al.   Scientific Reports

CRISPR/Cas9-mediated genome editing of Epstein-Barr virus in human cells

(2014) K.-S. Yuen et al.   JOURNAL OF GENERAL VIROLOGY

RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection

(2014) W. Hu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comprehensive analysis of the HEPN superfamily: identification of novel roles in intra-genomic conflicts, defense, pathogenesis and RNA processing

(2013) Vivek Anantharaman et al.   Biology Direct

The diversity of human RNA viruses

(2013) Mark EJ Woolhouse et al.   Future Virology

Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus

(2013) Hirotaka Ebina et al.   Scientific Reports

New World Bats Harbor Diverse Influenza A Viruses

(2013) Suxiang Tong et al.   PLoS Pathogens

Prediction and prevention of the next pandemic zoonosis

(2012) Stephen S Morse et al.   LANCET

RNA therapeutics: beyond RNA interference and antisense oligonucleotides

(2012) Ryszard Kole et al.   NATURE REVIEWS DRUG DISCOVERY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The Evolutionary Genetics of Emerging Viruses

(2010) Edward C. Holmes   Annual Review of Ecology Evolution and Systematics

Viral Mutation Rates

(2010) R. Sanjuan et al.   JOURNAL OF VIROLOGY

Advanced antisense therapies for postexposure protection against lethal filovirus infections

(2010) Travis K Warren et al.   NATURE MEDICINE

Short motif sequences determine the targets of the prokaryotic CRISPR defence system

(2009)   MICROBIOLOGY-SGM

Long-Term Control of HIV byCCR5Delta32/Delta32 Stem-Cell Transplantation

(2009) Gero Hütter et al.   NEW ENGLAND JOURNAL OF MEDICINE

CRISPR Interference Limits Horizontal Gene Transfer in Staphylococci by Targeting DNA

(2008) Luciano A. Marraffini et al.   SCIENCE

The biology of influenza viruses

(2008) Nicole M. Bouvier et al.   VACCINE