Genome Editing Strategies to Protect Livestock from Viral Infections

In Vivo Inhibition of Marek’s Disease Virus in Transgenic Chickens Expressing Cas9 and gRNA against ICP4

(2021) Arjun Challagulla et al.   Microorganisms

Cas9-expressing chickens and pigs as resources for genome editing in livestock

(2021) Beate Rieblinger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry

(2021) Yunkai Zhu et al.   Nature Communications

First GM pigs for allergies. Could xenotransplants be next?

(2021) Elie Dolgin   NATURE BIOTECHNOLOGY

A new strategy to develop pseudorabies virus-based bivalent vaccine with high immunogenicity of porcine circovirus type 2

(2021) Xiaofeng Wu et al.   VETERINARY MICROBIOLOGY

A Genome-Wide CRISPR/Cas9 Screen Reveals the Requirement of Host Sphingomyelin Synthase 1 for Infection with Pseudorabies Virus Mutant gD–Pass

(2021) Julia E. Hölper et al.   Viruses-Basel

Application of CRISPR-Cas9 Editing for Virus Engineering and the Development of Recombinant Viral Vaccines

(2021) Na Tang et al.   CRISPR Journal

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay

(2020) Xusheng Wang et al.   ACS Nano

Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/13a System

(2020) Chaoqun Yuan et al.   ANALYTICAL CHEMISTRY

The regulatory current status of plant breeding technologies in some Latin American and the Caribbean countries

(2020) Andrés Gatica-Arias   PLANT CELL TISSUE AND ORGAN CULTURE

Precise CRISPR/Cas9 editing of the NHE1 gene renders chickens resistant to the J subgroup of avian leukosis virus

(2020) Anna Koslová et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of A Triple Insert Live Avian Herpesvirus Vectored Vaccine Using CRISPR/Cas9-Based Gene Editing

(2020) Na Tang et al.   Vaccines

Highly Parallel Profiling of Cas9 Variant Specificity

(2020) Jonathan L. Schmid-Burgk et al.   MOLECULAR CELL

Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus

(2020) Stephen McCleary et al.   Scientific Reports

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

(2020) Shuhan Lu et al.   Cell Discovery

Abrogation of PRRSV infectivity by CRISPR-Cas13b-mediated viral RNA cleavage in mammalian cells

(2020) Jin Cui et al.   Scientific Reports

CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

(2020) Changzhi Zhao et al.   Nature Communications

CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance

(2020) Kui Xu et al.   eLife

Prevention of Avian Retrovirus Infection in Chickens Using CRISPR-Cas9 Delivered by Marek’s Disease Virus

(2020) Kai Li et al.   Molecular Therapy-Nucleic Acids

CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification

(2019) Benjamin L. Oakes et al.   CELL

MHC class II proteins mediate cross-species entry of bat influenza viruses

(2019) Umut Karakus et al.   NATURE

Introducing a Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas

(2019) Adam E. Dolan et al.   MOLECULAR CELL

The next generation of CRISPR–Cas technologies and applications

(2019) Adrian Pickar-Oliver et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

The ethics of genome editing in non-human animals: a systematic review of reasons reported in the academic literature

(2019) Nienke de Graeff et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Lessening of porcine epidemic diarrhoea virus susceptibility in piglets after editing of the CMP-N-glycolylneuraminic acid hydroxylase gene with CRISPR/Cas9 to nullify N-glycolylneuraminic acid expression

(2019) Ching-Fu Tu et al.   PLoS One

Visual detection of porcine reproductive and respiratory syndrome virus using CRISPR‐Cas13a

(2019) Yafei Chang et al.   Transboundary and Emerging Diseases

Highly Efficient Generation of Pigs Harboring a Partial Deletion of the CD163 SRCR5 Domain, Which Are Fully Resistant to Porcine Reproductive and Respiratory Syndrome Virus 2 Infection

(2019) Chunhe Guo et al.   Frontiers in Immunology

Aminopeptidase N-null neonatal piglets are protected from transmissible gastroenteritis virus but not porcine epidemic diarrhea virus

(2019) Lei Luo et al.   Scientific Reports

Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants

(2019) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas3 induces broad and unidirectional genome editing in human cells

(2019) Hiroyuki Morisaka et al.   Nature Communications

Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever

(2019) Jing Bai et al.   Frontiers in Microbiology

Simultaneous Deletion of Virulence Factors and Insertion of Antigens into the Infectious Laryngotracheitis Virus Using NHEJ-CRISPR/Cas9 and Cre–Lox System for Construction of a Stable Vaccine Vector

(2019) Mustafa Ozan Atasoy et al.   Vaccines

CD163 knockout pigs are fully resistant to highly pathogenic porcine reproductive and respiratory syndrome virus

(2018) Huaqiang Yang et al.   ANTIVIRAL RESEARCH

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

Genetically Modifying Livestock for Improved Welfare: A Path Forward

(2018) Adam Shriver et al.   JOURNAL OF AGRICULTURAL & ENVIRONMENTAL ETHICS

Is CRISPR an Ethical Game Changer?

(2018) Marcus Schultz-Bergin   JOURNAL OF AGRICULTURAL & ENVIRONMENTAL ETHICS

Invited review: Breeding and ethical perspectives on genetically modified and genome edited cattle

(2018) S. Eriksson et al.   JOURNAL OF DAIRY SCIENCE

Porcine Deltacoronavirus Engages the Transmissible Gastroenteritis Virus Functional Receptor Porcine Aminopeptidase N for Infectious Cellular Entry

(2018) Bin Wang et al.   JOURNAL OF VIROLOGY

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

A simple and rapid approach to develop recombinant avian herpesvirus vectored vaccines using CRISPR/Cas9 system

(2018) Na Tang et al.   VACCINE

The Application of NHEJ-CRISPR/Cas9 and Cre-Lox System in the Generation of Bivalent Duck Enteritis Virus Vaccine against Avian Influenza Virus

(2018) Pengxiang Chang et al.   Viruses-Basel

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Efficient transgene insertion in a pseudorabies virus vector by CRISPR/Cas9 and marker rescue-enforced recombination

(2018) Alexandra Hübner et al.   JOURNAL OF VIROLOGICAL METHODS

Resistance to coronavirus infection in amino peptidase N-deficient pigs

(2018) Kristin M. Whitworth et al.   TRANSGENIC RESEARCH

Genetically modified pigs are protected from classical swine fever virus

(2018) Zicong Xie et al.   PLoS Pathogens

Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing

(2017) Kepin Wang et al.   GENOME RESEARCH

Ethical issues when modelling brain disorders innon-human primates

(2017) Carolyn P Neuhaus   JOURNAL OF MEDICAL ETHICS

Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus

(2017) Shuqing Chen et al.   JOURNAL OF VIROLOGY

Editing mammalian genomes: ethical considerations

(2017) Andy Greenfield   MAMMALIAN GENOME

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

(2017) Dong Niu et al.   SCIENCE

Rural veterinarian's perception and practices in terms of biosecurity across three European countries

(2017) V. Renault et al.   Transboundary and Emerging Diseases

Genetically edited pigs lacking CD163 show no resistance following infection with the African swine fever virus isolate, Georgia 2007/1

(2017) Luca Popescu et al.   VIROLOGY

Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function

(2017) Christine Burkard et al.   PLoS Pathogens

Improved Vaccine against PRRSV: Current Progress and Future Perspective

(2017) Yuchen Nan et al.   Frontiers in Microbiology

Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection

(2017) Zhong Zou et al.   Scientific Reports

Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiratory Syndrome Virus

(2016) Kevin D. Wells et al.   JOURNAL OF VIROLOGY

CRISPR-Cas9 Can Inhibit HIV-1 Replication but NHEJ Repair Facilitates Virus Escape

(2016) Gang Wang et al.   MOLECULAR THERAPY

Gene-edited CRISPR mushroom escapes US regulation

(2016) Emily Waltz   NATURE

Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens

(2016) Caleb D. Marceau et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system

(2016) Yan-Dong Tang et al.   VIRUS RESEARCH

Animal nutrition in a 360-degree view and a framework for future R&D work: towards sustainable livestock production

(2016) Harinder P. S. Makkar   Animal Production Science

CRISPR/Cas9-Derived Mutations Both Inhibit HIV-1 Replication and Accelerate Viral Escape

(2016) Zhen Wang et al.   Cell Reports

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System

(2016) Joan K. Lunney et al.   Annual Review of Animal Biosciences

Mammalian interspecies substitution of immune modulatory alleles by genome editing

(2016) Simon G. Lillico et al.   Scientific Reports

CRISPR/Cas9-Mediated Immunity to Geminiviruses: Differential Interference and Evasion

(2016) Zahir Ali et al.   Scientific Reports

Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9

(2016) Kristine E. Yoder et al.   Scientific Reports

A simple and rapid approach to manipulate pseudorabies virus genome by CRISPR/Cas9 system

(2015) Aotian Xu et al.   BIOTECHNOLOGY LETTERS

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Efficiently Editing the Vaccinia Virus Genome by Using the CRISPR-Cas9 System

(2015) Ming Yuan et al.   JOURNAL OF VIROLOGY

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus

(2015) Kristin M Whitworth et al.   NATURE BIOTECHNOLOGY

A CRISPR-Cas9 gene drive system targeting female reproduction in the malaria mosquito vector Anopheles gambiae

(2015) Andrew Hammond et al.   NATURE BIOTECHNOLOGY

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

(2015) Hsin-Kai Liao et al.   Nature Communications

Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection

(2015) Shengwei Hu et al.   eLife

Genomic selection needs to be carefully assessed to meet specific requirements in livestock breeding programs

(2015) Elisabeth Jonas et al.   Frontiers in Genetics

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

High-Efficiency Targeted Editing of Large Viral Genomes by RNA-Guided Nucleases

(2014) Yanwei Bi et al.   PLoS Pathogens

Making Better Sense of Animal Disenhancement: A Reply to Henschke

(2014) Marcus Schultz-Bergin   NanoEthics

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Variation among sows in response to porcine reproductive and respiratory syndrome1

(2013) H. Rashidi et al.   JOURNAL OF ANIMAL SCIENCE

Nonconserved Tryptophan 38 of the Cell Surface Receptor for Subgroup J Avian Leukosis Virus Discriminates Sensitive from Resistant Avian Species

(2013) D. Kucerova et al.   JOURNAL OF VIROLOGY

Live pigs produced from genome edited zygotes

(2013) Simon G. Lillico et al.   Scientific Reports

The long view: 40 years of avian leukosis research

(2012) L. N. Payne et al.   AVIAN PATHOLOGY

Discovery of Seven Novel Mammalian and Avian Coronaviruses in the Genus Deltacoronavirus Supports Bat Coronaviruses as the Gene Source of Alphacoronavirus and Betacoronavirus and Avian Coronaviruses as the Gene Source of Gammacoronavirus and Deltacoronavirus

(2012) P. C. Y. Woo et al.   JOURNAL OF VIROLOGY

Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells

(2012) Maarten Holkers et al.   NUCLEIC ACIDS RESEARCH

Identification of Short Hairpin RNA Targeting Foot-And-Mouth Disease Virus with Transgenic Bovine Fetal Epithelium Cells

(2012) Hongmei Wang et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Challenges and Opportunities for the Control and Elimination of Porcine Reproductive and Respiratory Syndrome Virus

(2012) R. R. R. Rowland et al.   Transboundary and Emerging Diseases

Genetic analysis of tolerance to infections using random regressions: a simulation study

(2011) ANTTI KAUSE   Genetics Research

Species-Specific Variation in RELA Underlies Differences in NF- B Activity: a Potential Role in African Swine Fever Pathogenesis

(2011) C. J. Palgrave et al.   JOURNAL OF VIROLOGY

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Suppression of Avian Influenza Transmission in Genetically Modified Chickens

(2011) J. Lyall et al.   SCIENCE

Abstracts from the UC Davis Transgenic Animal Research Conference VIII

(2011)   TRANSGENIC RESEARCH

Regulation of Homologous Recombination in Eukaryotes

(2010) Wolf-Dietrich Heyer et al.   Annual Review of Genetics

Scavenger receptor CD163, a Jack-of-all-trades and potential target for cell-directed therapy

(2010) Hanne Van Gorp et al.   MOLECULAR IMMUNOLOGY

Identification of the CD163 Protein Domains Involved in Infection of the Porcine Reproductive and Respiratory Syndrome Virus

(2009) H. Van Gorp et al.   JOURNAL OF VIROLOGY

Retroviral delivery of RNA interference against Marek's disease virus in vivo

(2009) M. Chen et al.   POULTRY SCIENCE

Production of transgenic pigs that express porcine endogenous retrovirus small interfering RNAs

(2009) Jagdeece Ramsoondar et al.   XENOTRANSPLANTATION

Endogenous retroviruses

(2008) C. A. Wilson   CELLULAR AND MOLECULAR LIFE SCIENCES

Inhibition of Marek's disease virus replication by retroviral vector-based RNA interference

(2008) Mo Chen et al.   VIROLOGY

Knockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigs

(2008) Britta Dieckhoff et al.   XENOTRANSPLANTATION