Mechanistic Studies of an Automated Lipid Nanoparticle Reveal Critical Pharmaceutical Properties Associated with Enhanced mRNA Functional Delivery In Vitro and In Vivo

Membrane-destabilizing ionizable phospholipids for organ-selective mRNA delivery and CRISPR–Cas gene editing

(2021) Shuai Liu et al.   NATURE MATERIALS

Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles

(2021) Federica Sebastiani et al.   ACS Nano

Impact of lipid nanoparticle size on mRNA vaccine immunogenicity

(2021) Kimberly J. Hassett et al.   JOURNAL OF CONTROLLED RELEASE

Ionization and structural properties of mRNA lipid nanoparticles influence expression in intramuscular and intravascular administration

(2021) Manuel J. Carrasco et al.   Communications Biology

Engineering Caveolae-Targeted Lipid Nanoparticles To Deliver mRNA to the Lungs

(2020) Qing Li et al.   ACS Chemical Biology

Deconvoluting Lipid Nanoparticle Structure for Messenger RNA Delivery

(2020) Yulia Eygeris et al.   NANO LETTERS

Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing

(2020) Qiang Cheng et al.   Nature Nanotechnology

Gene-editing pipeline takes off

(2020) Asher Mullard   NATURE REVIEWS DRUG DISCOVERY

Systemic nanoparticle delivery of CRISPR-Cas9 ribonucleoproteins for effective tissue specific genome editing

(2020) Tuo Wei et al.   Nature Communications

Impact of mRNA chemistry and manufacturing process on innate immune activation

(2020) Jennifer Nelson et al.   Science Advances

Macropinocytosis as a cell entry route for peptide-functionalized and bystander nanoparticles

(2020) Yue-Xuan Li et al.   JOURNAL OF CONTROLLED RELEASE

Synthetic mRNA Encoding VEGF-A in Patients Undergoing Coronary Artery Bypass Grafting: Design of a Phase 2a Clinical Trial

(2020) Vesa Anttila et al.   Molecular Therapy-Methods & Clinical Development

Recent advances in chemical modifications of guide RNA, mRNA and donor template for CRISPR-mediated genome editing

(2020) Qiubing Chen et al.   ADVANCED DRUG DELIVERY REVIEWS

Branched-Tail Lipid Nanoparticles Potently Deliver mRNA In Vivo due to Enhanced Ionization at Endosomal pH

(2019) Khalid A. Hajj et al.   Small

Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery

(2019) Piotr S. Kowalski et al.   MOLECULAR THERAPY

Optimization of Lipid Nanoparticles for Intramuscular Administration of mRNA Vaccines

(2019) Kimberly J. Hassett et al.   Molecular Therapy-Nucleic Acids

Endocytic Profiling of Cancer Cell Models Reveals Critical Factors Influencing LNP-Mediated mRNA Delivery and Protein Expression

(2019) Edward J. Sayers et al.   MOLECULAR THERAPY

Delivery of mRNA vaccines with heterocyclic lipids increases anti-tumor efficacy by STING-mediated immune cell activation

(2019) Lei Miao et al.   NATURE BIOTECHNOLOGY

mRNA structure regulates protein expression through changes in functional half-life

(2019) David M. Mauger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

On the Formation and Morphology of Lipid Nanoparticles Containing Ionizable Cationic Lipids and siRNA

(2018) Jayesh A. Kulkarni et al.   ACS Nano

Identification of novel macropinocytosis inhibitors using a rational screen of FDA-approved drugs

(2018) Hui-Ping Lin et al.   BRITISH JOURNAL OF PHARMACOLOGY

mRNA vaccines — a new era in vaccinology

(2018) Norbert Pardi et al.   NATURE REVIEWS DRUG DISCOVERY

Successful reprogramming of cellular protein production through mRNA delivered by functionalized lipid nanoparticles

(2018) Marianna Yanez Arteta et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipid Nanoparticles Enabling Gene Therapies: From Concepts to Clinical Utility

(2018) Jayesh A. Kulkarni et al.   Nucleic Acid Therapeutics

A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing

(2018) Jonathan D. Finn et al.   Cell Reports

Nucleic acid nanomedicines in Phase II/III clinical trials: translation of nucleic acid therapies for reprogramming cells

(2018) Victoria LM Herrera et al.   Nanomedicine

Restoration of tumour-growth suppression in vivo via systemic nanoparticle-mediated delivery of PTEN mRNA

(2018) Mohammad Ariful Islam et al.   Nature Biomedical Engineering

Bacteriophage T7 transcription system: an enabling tool in synthetic biology

(2018) Wenya Wang et al.   BIOTECHNOLOGY ADVANCES

Lipid sensing by mTOR complexes viade novosynthesis of phosphatidic acid

(2017) Deepak Menon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Lipid Nanoparticle Systems for Enabling Gene Therapies

(2017) Pieter R. Cullis et al.   MOLECULAR THERAPY

Boosting Intracellular Delivery of Lipid Nanoparticle-Encapsulated mRNA

(2017) Siddharth Patel et al.   NANO LETTERS

Critical considerations for developing nucleic acid macromolecule based drug products

(2016) Bilikallahalli K. Muralidhara et al.   DRUG DISCOVERY TODAY

Influence of particle size on the in vivo potency of lipid nanoparticle formulations of siRNA

(2016) Sam Chen et al.   JOURNAL OF CONTROLLED RELEASE

Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy

(2016) Lena M. Kranz et al.   NATURE

Concise Review: Application of In Vitro Transcribed Messenger RNA for Cellular Engineering and Reprogramming: Progress and Challenges

(2016) Heidrun Steinle et al.   STEM CELLS

Microfluidic Mixing: A General Method for Encapsulating Macromolecules in Lipid Nanoparticle Systems

(2015) Alex K. K. Leung et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Optimization of Lipid Nanoparticle Formulations for mRNA Delivery in Vivo with Fractional Factorial and Definitive Screening Designs

(2015) Kevin J. Kauffman et al.   NANO LETTERS

Phosphatidic acid enhances mTOR signaling and resistance exercise induced hypertrophy

(2014) Jordan M Joy et al.   Nutrition & Metabolism

Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates

(2014) Y. Dong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biodegradable Lipids Enabling Rapidly Eliminated Lipid Nanoparticles for Systemic Delivery of RNAi Therapeutics

(2013) Martin A Maier et al.   MOLECULAR THERAPY

Image-based analysis of lipid nanoparticle–mediated siRNA delivery, intracellular trafficking and endosomal escape

(2013) Jerome Gilleron et al.   NATURE BIOTECHNOLOGY

Efficiency of siRNA delivery by lipid nanoparticles is limited by endocytic recycling

(2013) Gaurav Sahay et al.   NATURE BIOTECHNOLOGY

Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology

(2013) Stefan Tenzer et al.   Nature Nanotechnology

Multiparametric approach for the evaluation of lipid nanoparticles for siRNA delivery

(2013) C. A. Alabi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phosphatidic acid and lipid-sensing by mTOR

(2013) David A. Foster   TRENDS IN ENDOCRINOLOGY AND METABOLISM

Maximizing the Potency of siRNA Lipid Nanoparticles for Hepatic Gene Silencing In Vivo

(2012) Muthusamy Jayaraman et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Role of the Clathrin Terminal Domain in Regulating Coated Pit Dynamics Revealed by Small Molecule Inhibition

(2011) Lisa von Kleist et al.   CELL

Endosome maturation

(2011) Jatta Huotari et al.   EMBO JOURNAL

Molecular mechanism and physiological functions of clathrin-mediated endocytosis

(2011) Harvey T. McMahon et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Targeted Delivery of RNAi Therapeutics With Endogenous and Exogenous Ligand-Based Mechanisms

(2010) Akin Akinc et al.   MOLECULAR THERAPY

Rational design of cationic lipids for siRNA delivery

(2010) Sean C Semple et al.   NATURE BIOTECHNOLOGY

The SNX-PX-BAR Family in Macropinocytosis: The Regulation of Macropinosome Formation by SNX-PX-BAR Proteins

(2010) Jack T. H. Wang et al.   PLoS One

Lipid-like materials for low-dose, in vivo gene silencing

(2010) K. T. Love et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An Emerging Role of mTOR in Lipid Biosynthesis

(2009) Mathieu Laplante et al.   CURRENT BIOLOGY

Development of Lipidoid–siRNA Formulations for Systemic Delivery to the Liver

(2009) Akin Akinc et al.   MOLECULAR THERAPY

Defining Macropinocytosis

(2009) Markus C. Kerr et al.   TRAFFIC

Apoprotein E as a lipid transport and signaling protein in the blood, liver, and artery wall

(2008) Godfrey S. Getz et al.   JOURNAL OF LIPID RESEARCH

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY

A combinatorial library of lipid-like materials for delivery of RNAi therapeutics

(2008) Akin Akinc et al.   NATURE BIOTECHNOLOGY