Concise Review: Application of In Vitro Transcribed Messenger RNA for Cellular Engineering and Reprogramming: Progress and Challenges

Evading innate immunity in nonviral mRNA delivery: don’t shoot the messenger

(2016) Joke Devoldere et al.   DRUG DISCOVERY TODAY

Structural basis for m7G recognition and 2′-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I

(2016) Swapnil C. Devarkar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Strategies for modulating innate immune activation and protein production of in vitro transcribed mRNAs

(2016) Kristin H. Loomis et al.   Journal of Materials Chemistry B

Efficient Reprogramming of Human Fibroblasts and Blood-Derived Endothelial Progenitor Cells Using Nonmodified RNA for Reprogramming and Immune Evasion

(2015) Marco Alexander Poleganov et al.   HUMAN GENE THERAPY

Cystic Fibrosis Gene Therapy in the UK and Elsewhere

(2015) Uta Griesenbach et al.   HUMAN GENE THERAPY

A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2′O-Methylated Self RNA

(2015) Christine Schuberth-Wagner et al.   IMMUNITY

N 1 -methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice

(2015) Oliwia Andries et al.   JOURNAL OF CONTROLLED RELEASE

Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routes

(2015) Norbert Pardi et al.   JOURNAL OF CONTROLLED RELEASE

CD33-specific chimeric antigen receptor T cells exhibit potent preclinical activity against human acute myeloid leukemia

(2015) S S Kenderian et al.   LEUKEMIA

Synthetic mRNAs for manipulating cellular phenotypes: an overview

(2015) Elgar Susanne Quabius et al.   New Biotechnology

In VitroEvaluation of a Novel mRNA-Based Therapeutic Strategy for the Treatment of Patients Suffering from Alpha-1-Antitrypsin Deficiency

(2015) Tatjana Michel et al.   Nucleic Acid Therapeutics

Self-assembled Messenger RNA Nanoparticles (mRNA-NPs) for Efficient Gene Expression

(2015) Hyejin Kim et al.   Scientific Reports

Phase Ib study evaluating a self-adjuvanted mRNA cancer vaccine (RNActive®) combined with local radiation as consolidation and maintenance treatment for patients with stage IV non-small cell lung cancer

(2014) Martin Sebastian et al.   BMC CANCER

Japan stem-cell trial stirs envy

(2014) Sara Reardon et al.   NATURE

Antiviral immunity via RIG-I-mediated recognition of RNA bearing 5′-diphosphates

(2014) Delphine Goubau et al.   NATURE

A comparison of non-integrating reprogramming methods

(2014) Thorsten M Schlaeger et al.   NATURE BIOTECHNOLOGY

mRNA-based therapeutics — developing a new class of drugs

(2014) Ugur Sahin et al.   NATURE REVIEWS DRUG DISCOVERY

Tumorigenicity Studies of Induced Pluripotent Stem Cell (iPSC)-Derived Retinal Pigment Epithelium (RPE) for the Treatment of Age-Related Macular Degeneration

(2014) Hoshimi Kanemura et al.   PLoS One

Direct Reprogramming of Human Fibroblasts to Hepatocyte-Like Cells by Synthetic Modified mRNAs

(2014) Kamen P. Simeonov et al.   PLoS One

Cell therapy for macular degeneration—first phase I/II pluripotent stem cell-based clinical trial shows promise

(2014) ZhiGuo Chen et al.   Science China-Life Sciences

All Roads Lead to Induced Pluripotent Stem Cells: The Technologies of iPSC Generation

(2014) Kejin Hu   STEM CELLS AND DEVELOPMENT

Vectorology and Factor Delivery in Induced Pluripotent Stem Cell Reprogramming

(2014) Kejin Hu   STEM CELLS AND DEVELOPMENT

mRNA vaccine CV9103 and CV9104 for the treatment of prostate cancer

(2014) Steffen Rausch et al.   Human Vaccines & Immunotherapeutics

Messenger RNA- Versus Retrovirus-Based Induced Pluripotent Stem Cell Reprogramming Strategies: Analysis of Genomic Integrity

(2014) Clara Steichen et al.   Stem Cells Translational Medicine

Optimized conditions for successful transfection of human endothelial cells with in vitro synthesized and modified mRNA for induction of protein expression

(2014) Meltem Avci-Adali et al.   Journal of Biological Engineering

Direct Reprogramming of Fibroblasts into Myocytes to Reverse Fibrosis

(2013) Naoto Muraoka et al.   Annual Review of Physiology

mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10 to sites of inflammation

(2013) O. Levy et al.   BLOOD

Exogenous mRNA delivery and bioavailability in gene transfer mediated by piggyBac transposition

(2013) Solenne Bire et al.   BMC BIOTECHNOLOGY

Efficient Generation of Human iPSCs by a Synthetic Self-Replicative RNA

(2013) Naohisa Yoshioka et al.   Cell Stem Cell

Modified Foxp3 mRNA protects against asthma through an IL-10–dependent mechanism

(2013) Lauren E. Mays et al.   JOURNAL OF CLINICAL INVESTIGATION

Innate immune response and programmed cell death following carrier-mediated delivery of unmodified mRNA to respiratory cells

(2013) Oliwia Andries et al.   JOURNAL OF CONTROLLED RELEASE

Cystic fibrosis transmembrane conductance regulator-mRNA delivery: a novel alternative for cystic fibrosis gene therapy

(2013) Nadine Bangel-Ruland et al.   JOURNAL OF GENE MEDICINE

Modified mRNA directs the fate of heart progenitor cells and induces vascular regeneration after myocardial infarction

(2013) Lior Zangi et al.   NATURE BIOTECHNOLOGY

Sequential introduction of reprogramming factors reveals a time-sensitive requirement for individual factors and a sequential EMT–MET mechanism for optimal reprogramming

(2013) Xiaopeng Liu et al.   NATURE CELL BIOLOGY

Tumorigenicity as a clinical hurdle for pluripotent stem cell therapies

(2013) Andrew S Lee et al.   NATURE MEDICINE

Comparison of the molecular profiles of human embryonic and induced pluripotent stem cells of isogenic origin

(2013) Barbara S. Mallon et al.   Stem Cell Research

RNA-Based Tools for Nuclear Reprogramming and Lineage-Conversion: Towards Clinical Applications

(2013) Juan A. Bernal   Journal of Cardiovascular Translational Research

The Basic Principles of Chimeric Antigen Receptor Design

(2013) Michel Sadelain et al.   Cancer Discovery

Dendritic cell based vaccines for HIV infection

(2013) Felipe García et al.   Human Vaccines & Immunotherapeutics

Mesothelin-Specific Chimeric Antigen Receptor mRNA-Engineered T Cells Induce Antitumor Activity in Solid Malignancies

(2013) G. L. Beatty et al.   Cancer Immunology Research

Design of an Optimized Wilms’ Tumor 1 (WT1) mRNA Construct for Enhanced WT1 Expression and Improved Immunogenicity In Vitro and In Vivo

(2013) Daphné Benteyn et al.   Molecular Therapy-Nucleic Acids

The cytotoxic and immunogenic hurdles associated with non-viral mRNA-mediated reprogramming of human fibroblasts

(2012) Katharina Drews et al.   BIOMATERIALS

Assessing iPSC reprogramming methods for their suitability in translational medicine

(2012) Mahendra S. Rao et al.   JOURNAL OF CELLULAR BIOCHEMISTRY

Embryonic stem cell trials for macular degeneration: a preliminary report

(2012) Steven D Schwartz et al.   LANCET

Increased Erythropoiesis in Mice Injected With Submicrogram Quantities of Pseudouridine-containing mRNA Encoding Erythropoietin

(2012) Katalin Karikó et al.   MOLECULAR THERAPY

Systemic Delivery of Modified mRNA Encoding Herpes Simplex Virus 1 Thymidine Kinase for Targeted Cancer Gene Therapy

(2012) Yuhua Wang et al.   MOLECULAR THERAPY

In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes

(2012) Li Qian et al.   NATURE

Protective efficacy of in vitro synthesized, specific mRNA vaccines against influenza A virus infection

(2012) Benjamin Petsch et al.   NATURE BIOTECHNOLOGY

Lineage conversion methodologies meet the reprogramming toolbox

(2012) Ignacio Sancho-Martinez et al.   NATURE CELL BIOLOGY

Developing mRNA-vaccine technologies

(2012) Thomas Schlake et al.   RNA Biology

Feeder-Free Derivation of Human Induced Pluripotent Stem Cells with Messenger RNA

(2012) Luigi Warren et al.   Scientific Reports

Treatment of Advanced Leukemia in Mice with mRNA Engineered T Cells

(2011) David M. Barrett et al.   HUMAN GENE THERAPY

Immune Signaling by RIG-I-like Receptors

(2011) Yueh-Ming Loo et al.   IMMUNITY

Immunogenicity of induced pluripotent stem cells

(2011) Tongbiao Zhao et al.   NATURE

Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors

(2011) Sayaka Sekiya et al.   NATURE

Expression of therapeutic proteins after delivery of chemically modified mRNA in mice

(2011) Michael S D Kormann et al.   NATURE BIOTECHNOLOGY

Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5

(2011) Roland Züst et al.   NATURE IMMUNOLOGY

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

(2011) Katalin Karikó et al.   NUCLEIC ACIDS RESEARCH

Protein expression from exogenous mRNA: Uptake by receptor-mediated endocytosis and trafficking via the lysosomal pathway

(2011) Christina Lorenz et al.   RNA Biology

Reprogramming of human fibroblasts to pluripotent stem cells using mRNA of four transcription factors

(2010) Eduard Yakubov et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Particle size and activation threshold: a new dimension of danger signaling

(2010) L. Rettig et al.   BLOOD

Intranasal vaccination with messenger RNA as a new approach in gene therapy: Use against tuberculosis

(2010) Julio CC Lorenzi et al.   BMC BIOTECHNOLOGY

Generation of Induced Pluripotent Stem Cells from Human Terminally Differentiated Circulating T Cells

(2010) Tomohisa Seki et al.   Cell Stem Cell

Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA

(2010) Luigi Warren et al.   Cell Stem Cell

Direct conversion of human fibroblasts to multilineage blood progenitors

(2010) Eva Szabo et al.   NATURE

Direct conversion of fibroblasts to functional neurons by defined factors

(2010) Thomas Vierbuchen et al.   NATURE

Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins

(2010) Matthew Angel et al.   PLoS One

Activation of Pluripotency Genes in Human Fibroblast Cells by a Novel mRNA Based Approach

(2010) Jordan R. Plews et al.   PLoS One

Recognition of 5′ Triphosphate by RIG-I Helicase Requires Short Blunt Double-Stranded RNA as Contained in Panhandle of Negative-Strand Virus

(2009) Martin Schlee et al.   IMMUNITY

Activation of MDA5 Requires Higher-Order RNA Structures Generated during Virus Infection

(2009) A. Pichlmair et al.   JOURNAL OF VIROLOGY

Virus-free induction of pluripotency and subsequent excision of reprogramming factors

(2009) Keisuke Kaji et al.   NATURE

piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells

(2009) Knut Woltjen et al.   NATURE

Activation of innate immune antiviral responses by Nod2

(2009) Ahmed Sabbah et al.   NATURE IMMUNOLOGY

5'-triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I

(2009) A. Schmidt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences

(2009) J. Yu et al.   SCIENCE

Use of the viral 2A peptide for bicistronic expression in transgenic mice

(2008) Georgios Trichas et al.   BMC BIOLOGY

Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid–inducible gene-I and melanoma differentiation–associated gene 5

(2008) Hiroki Kato et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY

In vivo reprogramming of adult pancreatic exocrine cells to β-cells

(2008) Qiao Zhou et al.   NATURE

Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2

(2008) Danwei Huangfu et al.   NATURE BIOTECHNOLOGY

Multifunctional deadenylase complexes diversify mRNA control

(2008) Aaron C. Goldstrohm et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Nucleoside modifications modulate activation of the protein kinase PKR in an RNA structure-specific manner

(2008) S. R. Nallagatla et al.   RNA

Induced Pluripotent Stem Cells Generated Without Viral Integration

(2008) M. Stadtfeld et al.   SCIENCE