Synthetic biology: applying biological circuits beyond novel therapies

Synthetic Biology—Toward Therapeutic Solutions

(2016) Viktor Haellman et al.   JOURNAL OF MOLECULAR BIOLOGY

The Expanded Genetic Alphabet

(2015) Denis A. Malyshev et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Human whole-blood culture system for ex vivo characterization of designer-cell function

(2015) Lina Schukur et al.   BIOTECHNOLOGY AND BIOENGINEERING

Current State of Circulating MicroRNAs as Cancer Biomarkers

(2015) Y. He et al.   CLINICAL CHEMISTRY

Novel theranostic agents for next-generation personalized medicine: small molecules, nanoparticles, and engineered mammalian cells

(2015) Ryosuke Kojima et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

MicroRNA regulation of allergic inflammation and asthma

(2015) Heather H Pua et al.   CURRENT OPINION IN IMMUNOLOGY

CAR therapy: the CD19 paradigm

(2015) Michel Sadelain   JOURNAL OF CLINICAL INVESTIGATION

A synthetic cGMP-sensitive gene switch providing Viagra ® -controlled gene expression in mammalian cells and mice

(2015) Taeuk Kim et al.   METABOLIC ENGINEERING

Expanding the Biologist’s Toolkit with CRISPR-Cas9

(2015) Samuel H. Sternberg et al.   MOLECULAR CELL

Combinatorial gene regulation by modulation of relative pulse timing

(2015) Yihan Lin et al.   NATURE

Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

(2015) Liliana Wroblewska et al.   NATURE BIOTECHNOLOGY

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers

(2015) Isaac B Hilton et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

DNA sense-and-respond protein modules for mammalian cells

(2015) Shimyn Slomovic et al.   NATURE METHODS

Cosmetics-triggered percutaneous remote control of transgene expression in mice

(2015) Hui Wang et al.   NUCLEIC ACIDS RESEARCH

Remote control of therapeutic T cells through a small molecule-gated chimeric receptor

(2015) C.-Y. Wu et al.   SCIENCE

Synthetic biology in cell-based cancer immunotherapy

(2015) Deboki Chakravarti et al.   TRENDS IN BIOTECHNOLOGY

Cell encapsulation: technical and clinical advances

(2015) Gorka Orive et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish

(2015) Hannes M. Beyer et al.   ACS Synthetic Biology

Tetracyclines Disturb Mitochondrial Function across Eukaryotic Models: A Call for Caution in Biomedical Research

(2015) Norman Moullan et al.   Cell Reports

Precision cancer mouse models through genome editing with CRISPR-Cas9

(2015) Haiwei Mou et al.   Genome Medicine

Engineering dynamic cell cycle control with synthetic small molecule-responsive RNA devices

(2015) Kathy Y. Wei et al.   Journal of Biological Engineering

Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation

(2015) Diego Balboa et al.   Stem Cell Reports

CellNet: Network Biology Applied to Stem Cell Engineering

(2014) Patrick Cahan et al.   CELL

Recent applications of synthetic biology tools for yeast metabolic engineering

(2014) Michael K. Jensen et al.   FEMS YEAST RESEARCH

Engineered UV-A light-responsive gene expression system for measuring sun cream efficacy in mammalian cell culture

(2014) Markus Wieland et al.   JOURNAL OF BIOTECHNOLOGY

Construction of a yeast chromosome

(2014) Daniel G. Gibson et al.   NATURE

Early lineage restriction in temporally distinct populations of Mesp1 progenitors during mammalian heart development

(2014) Fabienne Lescroart et al.   NATURE CELL BIOLOGY

Digital switching in a biosensor circuit via programmable timing of gene availability

(2014) Nicolas Lapique et al.   Nature Chemical Biology

A quantitative framework for the forward design of synthetic miRNA circuits

(2014) Ryan J Bloom et al.   NATURE METHODS

A general design strategy for protein-responsive riboswitches in mammalian cells

(2014) Simon Ausländer et al.   NATURE METHODS

A brief history of synthetic biology

(2014) D. Ewen Cameron et al.   NATURE REVIEWS MICROBIOLOGY

Antagonistic control of a dual-input mammalian gene switch by food additives

(2014) Mingqi Xie et al.   NUCLEIC ACIDS RESEARCH

Engineering allostery

(2014) Srivatsan Raman et al.   TRENDS IN GENETICS

Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells

(2014) Dominik Niopek et al.   Nature Communications

Mind-controlled transgene expression by a wireless-powered optogenetic designer cell implant

(2014) Marc Folcher et al.   Nature Communications

A designer cell-based histamine-specific human allergy profiler

(2014) David Ausländer et al.   Nature Communications

Orthogonal Optogenetic Triple-Gene Control in Mammalian Cells

(2014) Konrad Müller et al.   ACS Synthetic Biology

An overview of the diverse roles of G-protein coupled receptors (GPCRs) in the pathophysiology of various human diseases

(2013) Boon Chin Heng et al.   BIOTECHNOLOGY ADVANCES

A light-triggered protein secretion system

(2013) Daniel Chen et al.   JOURNAL OF CELL BIOLOGY

Protein Scaffold-Activated Protein Trans-Splicing in Mammalian Cells

(2013) Daniel F. Selgrade et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells

(2013) Konrad Müller et al.   NUCLEIC ACIDS RESEARCH

Multi-chromatic control of mammalian gene expression and signaling

(2013) Konrad Müller et al.   NUCLEIC ACIDS RESEARCH

Reward-based hypertension control by a synthetic brain-dopamine interface

(2013) K. Rossger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A closed-loop synthetic gene circuit for the treatment of diet-induced obesity in mice

(2013) Katrin Rössger et al.   Nature Communications

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

Dynamics and Memory of Heterochromatin in Living Cells

(2012) Nathaniel A. Hathaway et al.   CELL

A Synthetic Biology Framework for Programming Eukaryotic Transcription Functions

(2012) Ahmad S. Khalil et al.   CELL

Synthetic memory circuits for tracking human cell fate

(2012) D. R. Burrill et al.   GENES & DEVELOPMENT

A novel reporter system for bacterial and mammalian cells based on the non-ribosomal peptide indigoidine

(2012) Marius Müller et al.   METABOLIC ENGINEERING

Engineering of ribozyme-based riboswitches for mammalian cells

(2012) Markus Wieland et al.   METHODS

Synthetic two-way communication between mammalian cells

(2012) William Bacchus et al.   NATURE BIOTECHNOLOGY

TULIPs: tunable, light-controlled interacting protein tags for cell biology

(2012) Devin Strickland et al.   NATURE METHODS

Three-dimensionally designed protein-responsive RNA devices for cell signaling regulation

(2012) Shunnichi Kashida et al.   NUCLEIC ACIDS RESEARCH

Distance and Helical Phase Dependence of Synergistic Transcription Activation in cis-Regulatory Module

(2012) Qilai Huang et al.   PLoS One

Pharmaceutically controlled designer circuit for the treatment of the metabolic syndrome

(2012) H. Ye et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Radio-Wave Heating of Iron Oxide Nanoparticles Can Regulate Plasma Glucose in Mice

(2012) S. A. Stanley et al.   SCIENCE

Optical Control of Protein Activity by Fluorescent Protein Domains

(2012) X. X. Zhou et al.   SCIENCE

Supporting conditional mouse mutagenesis with a comprehensive cre characterization resource

(2012) Caleb S. Heffner et al.   Nature Communications

Intersectional Cre Driver Lines Generated Using Split-Intein Mediated Split-Cre Reconstitution

(2012) Ping Wang et al.   Scientific Reports

The use of light for engineered control and reprogramming of cellular functions

(2011) William Bacchus et al.   CURRENT OPINION IN BIOTECHNOLOGY

Small-molecule displacement of a cryptic degron causes conditional protein degradation

(2011) Kimberly M Bonger et al.   Nature Chemical Biology

Inducible Apoptosis as a Safety Switch for Adoptive Cell Therapy

(2011) Antonio Di Stasi et al.   NEW ENGLAND JOURNAL OF MEDICINE

The food additive vanillic acid controls transgene expression in mammalian cells and mice

(2011) Marc Gitzinger et al.   NUCLEIC ACIDS RESEARCH

A Synthetic Optogenetic Transcription Device Enhances Blood-Glucose Homeostasis in Mice

(2011) H. Ye et al.   SCIENCE

Multi-Input RNAi-Based Logic Circuit for Identification of Specific Cancer Cells

(2011) Z. Xie et al.   SCIENCE

Estradiol-Treated Female Mice as Surrogate Hosts for Neisseria gonorrhoeae Genital Tract Infections

(2011) Ann E. Jerse et al.   Frontiers in Microbiology

A high-throughput screening system for G-protein-coupled receptors using β-lactamase enzyme complementation technology

(2010) Chuan-ke Zhao et al.   ACTA PHARMACOLOGICA SINICA

Rewiring Cells: Synthetic Biology as a Tool to Interrogate the Organizational Principles of Living Systems

(2010) Caleb J. Bashor et al.   Annual Review of Biophysics

Intestinal Crypt Homeostasis Results from Neutral Competition between Symmetrically Dividing Lgr5 Stem Cells

(2010) Hugo J. Snippert et al.   CELL

Photomodulation of Protein Trans-Splicing Through Backbone Photocaging of the DnaE Split Intein

(2010) Luis Berrade et al.   CHEMBIOCHEM

A designer network coordinating bovine artificial insemination by ovulation-triggered release of implanted sperms

(2010) Christian Kemmer et al.   JOURNAL OF CONTROLLED RELEASE

A tunable dual-promoter integrator for targeting of cancer cells

(2010) Lior Nissim et al.   Molecular Systems Biology

Self-sufficient control of urate homeostasis in mice by a synthetic circuit

(2010) Christian Kemmer et al.   NATURE BIOTECHNOLOGY

Optogenetic control of heart muscle in vitro and in vivo

(2010) Tobias Bruegmann et al.   NATURE METHODS

Rapid blue-light–mediated induction of protein interactions in living cells

(2010) Matthew J Kennedy et al.   NATURE METHODS

Rationally designed logic integration of regulatory signals in mammalian cells

(2010) Madeleine Leisner et al.   Nature Nanotechnology

Directed molecular evolution of DREADDs: a generic approach to creating next-generation RASSLs

(2010) Shuyun Dong et al.   Nature Protocols

An engineered mammalian band-pass network

(2010) D. Greber et al.   NUCLEIC ACIDS RESEARCH

A synthetic low-frequency mammalian oscillator

(2010) Marcel Tigges et al.   NUCLEIC ACIDS RESEARCH

Design of small molecule-responsive microRNAs based on structural requirements for Drosha processing

(2010) Chase L. Beisel et al.   NUCLEIC ACIDS RESEARCH

Genetic control of mammalian T-cell proliferation with synthetic RNA regulatory systems

(2010) Y. Y. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic design of strong promoters

(2010) M. R. Schlabach et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming Cellular Behavior with RNA Controllers Responsive to Endogenous Proteins

(2010) S. J. Culler et al.   SCIENCE

In vivo delivery of a microRNA-regulated transgene induces antigen-specific regulatory T cells and promotes immunologic tolerance

(2009) A. Annoni et al.   BLOOD

Temporally precise in vivo control of intracellular signalling

(2009) Raag D. Airan et al.   NATURE

A genetically encoded photoactivatable Rac controls the motility of living cells

(2009) Yi I. Wu et al.   NATURE

A tunable synthetic mammalian oscillator

(2009) Marcel Tigges et al.   NATURE

Induction of protein-protein interactions in live cells using light

(2009) Masayuki Yazawa et al.   NATURE BIOTECHNOLOGY

Split-Cre Complementation Indicates Coincident Activity of Different Genes In Vivo

(2009) Johannes Hirrlinger et al.   PLoS One

Intron length increases oscillatory periods of gene expression in animal cells

(2008) I. A. Swinburne et al.   GENES & DEVELOPMENT

Engineered lentiviral vector targeting astrocytesIn vivo

(2008) Angélique Colin et al.   GLIA

Chemical control of protein stability and function in living mice

(2008) Laura A Banaszynski et al.   NATURE MEDICINE

A synthetic mammalian gene circuit reveals antituberculosis compounds

(2008) W. Weber et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Higher-Order Cellular Information Processing with Synthetic RNA Devices

(2008) M. N. Win et al.   SCIENCE