Targeted protein degradation using intracellular antibodies and its application to neurodegenerative disease

Target-induced clustering activates Trim-Away of pathogens and proteins

(2021) Jingwei Zeng et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Effect of reductions in amyloid levels on cognitive change in randomized trials: instrumental variable meta-analysis

(2021) Sarah F Ackley et al.   BMJ-British Medical Journal

Tau antibody isotype induces differential effects following passive immunisation of tau transgenic mice

(2021) Rinie Bajracharya et al.   Acta Neuropathologica Communications

α-Synuclein antisense oligonucleotides as a disease-modifying therapy for Parkinson’s disease

(2021) Tracy A. Cole et al.   JCI Insight

Persistent repression of tau in the brain using engineered zinc finger protein transcription factors

(2021) Susanne Wegmann et al.   Science Advances

Administration of AAV-Alpha Synuclein NAC Antibody Improves Locomotor Behavior in Rats Overexpressing Alpha Synuclein

(2021) Yun-Hsiang Chen et al.   Genes

Direct targeting of oncogenic RAS mutants with a tumor-specific cytosol-penetrating antibody inhibits RAS mutant–driven tumor growth

(2020) Seung-Min Shin et al.   Science Advances

Gene therapy for neurodegenerative disorders: advances, insights and prospects

(2020) Wei Chen et al.   Acta Pharmaceutica Sinica B

Antibody RING-Mediated Destruction of Endogenous Proteins

(2020) Adel F.M. Ibrahim et al.   MOLECULAR CELL

Cyclophilin A Prevents HIV-1 Restriction in Lymphocytes by Blocking Human TRIM5α Binding to the Viral Core

(2020) Anastasia Selyutina et al.   Cell Reports

The physiological roles of tau and Aβ: implications for Alzheimer’s disease pathology and therapeutics

(2020) Sarah A. Kent et al.   ACTA NEUROPATHOLOGICA

Anti-tau scFvs targeted to the cytoplasm or secretory pathway variably modify pathology and neurodegenerative phenotypes

(2020) Marshall S. Goodwin et al.   MOLECULAR THERAPY

Initiation of a conserved trophectoderm program in human, cow and mouse embryos

(2020) Claudia Gerri et al.   NATURE

X-Linked Huwe1 Is Essential for Oocyte Maturation and Preimplantation Embryo Development

(2020) Alaa A. Eisa et al.   iScience

Viral nucleoprotein antibodies activate TRIM21 and induce T cell immunity

(2020) Sarah L Caddy et al.   EMBO JOURNAL

Cell-Permeant Bioadaptors for Cytosolic Delivery of Native Antibodies: A “Mix-and-Go” Approach

(2020) Shubo Du et al.   ACS Central Science

Trim-Away mediated knock down uncovers a new function for Lbh during gastrulation of Xenopus laevis.

(2020) Emma Weir et al.   DEVELOPMENTAL BIOLOGY

SNAP23 is required for constitutive and regulated exocytosis in mouse oocytes†

(2019) Lisa M Mehlmann et al.   BIOLOGY OF REPRODUCTION

Adenovirus Entry: From Infection to Immunity

(2019) Urs F. Greber et al.   Annual Review of Virology

The Role of Antibodies and Their Receptors in Protection Against Ordered Protein Assembly in Neurodegeneration

(2019) Taxiarchis Katsinelos et al.   Frontiers in Immunology

Loss of CENPF leads to developmental failure in mouse embryos

(2019) Cheng-Jie Zhou et al.   CELL CYCLE

Prion-like spreading of Alzheimer’s disease within the brain’s connectome

(2019) Sveva Fornari et al.   Journal of the Royal Society Interface

Targeting tauopathy with engineered tau-degrading intrabodies

(2019) Gilbert Gallardo et al.   Molecular Neurodegeneration

Cytosolic delivery of inhibitory antibodies with cationic lipids

(2019) Hejia Henry Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cyclophilin A protects HIV-1 from restriction by human TRIM5α

(2019) Kyusik Kim et al.   Nature Microbiology

Size and affinity kinetics of nanobodies influence targeting and penetration of solid tumours

(2019) Pieterjan Debie et al.   JOURNAL OF CONTROLLED RELEASE

Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model

(2019) Lien Nguyen et al.   NEURON

Intracellular delivery of therapeutic antibodies into specific cells using antibody-peptide fusions

(2019) Julie Gaston et al.   Scientific Reports

Antibody glycoengineering strategies in mammalian cells

(2018) Qiong Wang et al.   BIOTECHNOLOGY AND BIOENGINEERING

AAV5-miHTT Gene Therapy Demonstrates Broad Distribution and Strong Human Mutant Huntingtin Lowering in a Huntington’s Disease Minipig Model

(2018) Melvin M. Evers et al.   MOLECULAR THERAPY

The Neurotropic Properties of AAV-PHP.B Are Limited to C57BL/6J Mice

(2018) Juliette Hordeaux et al.   MOLECULAR THERAPY

Tau-targeting therapies for Alzheimer disease

(2018) Erin E. Congdon et al.   Nature Reviews Neurology

Intravenous administration of the adeno-associated virus-PHP.B capsid fails to upregulate transduction efficiency in the marmoset brain

(2018) Yasunori Matsuzaki et al.   NEUROSCIENCE LETTERS

The structural basis of nanobody unfolding reversibility and thermoresistance

(2018) Patrick Kunz et al.   Scientific Reports

A cell-penetrating whole molecule antibody targeting intracellular HBx suppresses hepatitis B virus via TRIM21-dependent pathway

(2018) Jun-Fang Zhang et al.   Theranostics

Intracellular antibody signalling is regulated by phosphorylation of the Fc receptor TRIM21

(2018) Claire Dickson et al.   eLife

Protein misfolding, aggregation, and conformational strains in neurodegenerative diseases

(2018) Claudio Soto et al.   NATURE NEUROSCIENCE

Acute and rapid degradation of endogenous proteins by Trim-Away

(2018) Dean Clift et al.   Nature Protocols

Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems

(2017) Ken Y Chan et al.   NATURE NEUROSCIENCE

Cytosolic Fc receptor TRIM21 inhibits seeded tau aggregation

(2017) William A. McEwan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Tau reduction prevents neuronal loss and reverses pathological tau deposition and seeding in mice with tauopathy

(2017) Sarah L. DeVos et al.   Science Translational Medicine

Antibody targeting intracellular oncogenic Ras mutants exerts anti-tumour effects after systemic administration

(2017) Seung-Min Shin et al.   Nature Communications

Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism

(2016) Wenchun Fan et al.   ANTIVIRAL RESEARCH

Endosomal acidic pH-induced conformational changes of a cytosol-penetrating antibody mediate endosomal escape

(2016) Ji-Sun Kim et al.   JOURNAL OF CONTROLLED RELEASE

Safety and durability of effect of contralateral-eye administration of AAV2 gene therapy in patients with childhood-onset blindness caused by RPE65 mutations: a follow-on phase 1 trial

(2016) Jean Bennett et al.   LANCET

The antibody aducanumab reduces Aβ plaques in Alzheimer’s disease

(2016) Jeff Sevigny et al.   NATURE

Defects in lysosomal maturation facilitate the activation of innate sensors in systemic lupus erythematosus

(2016) Andrew J. Monteith et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Antibody-Mediated Targeting of Tau In Vivo Does Not Require Effector Function and Microglial Engagement

(2016) Seung-Hye Lee et al.   Cell Reports

An affinity-directed protein missile system for targeted proteolysis

(2016) Luke J. Fulcher et al.   Open Biology

NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies

(2016) Sandrine Moutel et al.   eLife

Antibody against early driver of neurodegeneration cis P-tau blocks brain injury and tauopathy

(2015) Asami Kondo et al.   NATURE

Sequential ubiquitination and deubiquitination enzymes synchronize the dual sensor and effector functions of TRIM21

(2015) Adam J. Fletcher et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microglial internalization and degradation of pathological tau is enhanced by an anti-tau monoclonal antibody

(2015) Wenjie Luo et al.   Scientific Reports

TRIM21 Promotes cGAS and RIG-I Sensing of Viral Genomes during Infection by Antibody-Opsonized Virus

(2015) Ruth E. Watkinson et al.   PLoS Pathogens

Anti-Yo Antibody Uptake and Interaction with Its Intracellular Target Antigen Causes Purkinje Cell Death in Rat Cerebellar Slice Cultures: A Possible Mechanism for Paraneoplastic Cerebellar Degeneration in Humans with Gynecological or Breast Cancers

(2015) John E. Greenlee et al.   PLoS One

Cell-Penetrating Peptides: Design, Synthesis, and Applications

(2014) Dana Maria Copolovici et al.   ACS Nano

Neuronal uptake of tau/pS422 antibody and reduced progression of tau pathology in a mouse model of Alzheimer‘s disease

(2014) Ludovic Collin et al.   BRAIN

Neuronal uptake of anti-Hu antibody, but not anti-Ri antibody, leads to cell death in brain slice cultures

(2014) John E Greenlee et al.   Journal of Neuroinflammation

A general strategy for generating intact, full-length IgG antibodies that penetrate into the cytosol of living cells

(2014) Dong-Ki Choi et al.   mAbs

Nanobody-based products as research and diagnostic tools

(2014) Thomas De Meyer et al.   TRENDS IN BIOTECHNOLOGY

An Evolutionary Screen Highlights Canonical and Noncanonical Candidate Antiviral Genes within the Primate TRIM Gene Family

(2013) Ray Malfavon-Borja et al.   Genome Biology and Evolution

Antibody Uptake into Neurons Occurs Primarily via Clathrin-dependent Fcγ Receptor Endocytosis and Is a Prerequisite for Acute Tau Protein Clearance

(2013) Erin E. Congdon et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Two Novel Tau Antibodies Targeting the 396/404 Region Are Primarily Taken Up by Neurons and Reduce Tau Protein Pathology

(2013) Jiaping Gu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21

(2013) William A McEwan et al.   NATURE IMMUNOLOGY

Intracellular antibody receptor TRIM21 prevents fatal viral infection

(2013) M. Vaysburd et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulation of Virus Neutralization and the Persistent Fraction by TRIM21

(2012) W. A. McEwan et al.   JOURNAL OF VIROLOGY

Fusion to a highly charged proteasomal retargeting sequence increases soluble cytoplasmic expression and efficacy of diverse anti-synuclein intrabodies

(2012) Shubhada N. Joshi et al.   mAbs

Passive Immunization with Anti-Tau Antibodies in Two Transgenic Models

(2011) Xiyun Chai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fluorescent fusion protein knockout mediated by anti-GFP nanobody

(2011) Emmanuel Caussinus et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Bifunctional Anti-Huntingtin Proteasome-Directed Intrabodies Mediate Efficient Degradation of Mutant Huntingtin Exon 1 Protein Fragments

(2011) David C. Butler et al.   PLoS One

Single Domain Intracellular Antibodies from Diverse Libraries

(2010) Tomoyuki Tanaka et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Antibodies mediate intracellular immunity through tripartite motif-containing 21 (TRIM21)

(2010) D. L. Mallery et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The propagation of prion-like protein inclusions in neurodegenerative diseases

(2010) Michel Goedert et al.   TRENDS IN NEUROSCIENCES

A multi-Fc-species system for recombinant antibody production

(2009) Sandrine Moutel et al.   BMC BIOTECHNOLOGY

Five siRNAs Targeting Three SNPs May Provide Therapy for Three-Quarters of Huntington's Disease Patients

(2009) Edith L. Pfister et al.   CURRENT BIOLOGY

Cerebellar Purkinje cells incorporate immunoglobulins and immunotoxins in vitro: implications for human neurological disease and immunotherapeutics

(2009) Kenneth E Hill et al.   Journal of Neuroinflammation

Intrabody Gene Therapy Ameliorates Motor, Cognitive, and Neuropathological Symptoms in Multiple Mouse Models of Huntington's Disease

(2009) A. L. Southwell et al.   JOURNAL OF NEUROSCIENCE

DARPins: A new generation of protein therapeutics

(2008) Michael T. Stumpp et al.   DRUG DISCOVERY TODAY

TRIM21 is an IgG receptor that is structurally, thermodynamically, and kinetically conserved

(2008) A. H. Keeble et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Independent evolution of an antiviral TRIMCyp in rhesus macaques

(2008) S. J. Wilson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single treatment with RNAi against prion protein rescues early neuronal dysfunction and prolongs survival in mice with prion disease

(2008) M. D. White et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

An scFv Intrabody against the Nonamyloid Component of α-Synuclein Reduces Intracellular Aggregation and Toxicity

(2007) Sandra M. Lynch et al.   JOURNAL OF MOLECULAR BIOLOGY