Antibody glycoengineering strategies in mammalian cells

Arabinosylation of recombinant human immunoglobulin-based protein therapeutics

(2017) Patrick Hossler et al.   mAbs

Modulating IgG effector function by Fc glycan engineering

(2017) Tiezheng Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Immunoregulatory Roles of Antibody Glycosylation

(2017) Madeleine F. Jennewein et al.   TRENDS IN IMMUNOLOGY

Integrated Genome and Protein Editing Swaps α -2,6 Sialylation for α -2,3 Sialic Acid on Recombinant Antibodies from CHO

(2017) Cheng-yu Chung et al.   Biotechnology Journal

Fc-Galactosylation of Human Immunoglobulin Gamma Isotypes Improves C1q Binding and Enhances Complement-Dependent Cytotoxicity

(2017) Benjamin Peschke et al.   Frontiers in Immunology

Glycoengineering of pertuzumab and its impact on the pharmacokinetic/pharmacodynamic properties

(2017) Cheng Luo et al.   Scientific Reports

Enhanced sialylation of a human chimeric IgG1 variant produced in human and rodent cell lines

(2016) Yusuke Mimura et al.   JOURNAL OF IMMUNOLOGICAL METHODS

The immunoglobulin G1 N-glycan composition affects binding to each low affinity Fc γ receptor

(2016) Ganesh P. Subedi et al.   mAbs

Fc-galactosylation modulates antibody-dependent cellular cytotoxicity of therapeutic antibodies

(2016) Marco Thomann et al.   MOLECULAR IMMUNOLOGY

Low glucose depletes glycan precursors, reduces site occupancy and galactosylation of a monoclonal antibody in CHO cell culture

(2015) Carina Villacrés et al.   Biotechnology Journal

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

Chinese hamster ovary (CHO) host cell engineering to increase sialylation of recombinant therapeutic proteins by modulating sialyltransferase expression

(2015) Nan Lin et al.   BIOTECHNOLOGY PROGRESS

Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibody

(2015) Tao Sun et al.   ENGINEERING IN LIFE SCIENCES

Fc glycans of therapeutic antibodies as critical quality attributes

(2015) Dietmar Reusch et al.   GLYCOBIOLOGY

Metabolic markers associated with high mannose glycan levels of therapeutic recombinant monoclonal antibodies

(2015) Sohye Kang et al.   JOURNAL OF BIOTECHNOLOGY

Sialylation of IgG Fc domain impairs complement-dependent cytotoxicity

(2015) Isaak Quast et al.   JOURNAL OF CLINICAL INVESTIGATION

The Current Status and Prospects of Antibody Engineering for Therapeutic Use: Focus on Glycoengineering Technology

(2015) Rinpei Niwa et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Antibody Glycosylation and Its Impact on the Pharmacokinetics and Pharmacodynamics of Monoclonal Antibodies and Fc-Fusion Proteins

(2015) Liming Liu   JOURNAL OF PHARMACEUTICAL SCIENCES

Production of α2,6-sialylated IgG1 in CHO cells

(2015) Céline Raymond et al.   mAbs

Engineered CHO cells for production of diverse, homogeneous glycoproteins

(2015) Zhang Yang et al.   NATURE BIOTECHNOLOGY

Controlled tetra-Fc sialylation of IVIg results in a drug candidate with consistent enhanced anti-inflammatory activity

(2015) Nathaniel Washburn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A common glycan structure on immunoglobulin G for enhancement of effector functions

(2015) Chin-Wei Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Structural Role of Antibody N-Glycosylation in Receptor Interactions

(2015) Ganesh P. Subedi et al.   STRUCTURE

Site-specific integration in CHO cells mediated by CRISPR/Cas9 and homology-directed DNA repair pathway

(2015) Jae Seong Lee et al.   Scientific Reports

Low glucose depletes glycan precursors, reduces site occupancy and galactosylation of a monoclonal antibody in CHO cell culture

(2015) Carina Villacrés et al.   Biotechnology Journal

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment

(2015) Lise Marie Grav et al.   Biotechnology Journal

In Vitro Glycoengineering of IgG1 and Its Effect on Fc Receptor Binding and ADCC Activity

(2015) Marco Thomann et al.   PLoS One

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool

(2014) Carlotta Ronda et al.   BIOTECHNOLOGY AND BIOENGINEERING

Recent advances in the understanding of biological implications and modulation methodologies of monoclonal antibody N-linked high mannose glycans

(2014) Helen H. Shi et al.   BIOTECHNOLOGY AND BIOENGINEERING

Broad requirement for terminal sialic acid residues and FcγRIIB for the preventive and therapeutic activity of intravenous immunoglobulins in vivo

(2014) Inessa Schwab et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Immunoglobulin G (IgG) Fab Glycosylation Analysis Using a New Mass Spectrometric High-throughput Profiling Method Reveals Pregnancy-associated Changes

(2014) Albert Bondt et al.   MOLECULAR & CELLULAR PROTEOMICS

GlycoDelete engineering of mammalian cells simplifies N-glycosylation of recombinant proteins

(2014) Leander Meuris et al.   NATURE BIOTECHNOLOGY

Simply better glycoproteins

(2014) Bernd Lepenies et al.   NATURE BIOTECHNOLOGY

IgG Subclasses and Allotypes: From Structure to Effector Functions

(2014) Gestur Vidarsson et al.   Frontiers in Immunology

Engineering Chinese Hamster Ovary (CHO) cells for producing recombinant proteins with simple glycoforms by zinc-finger nuclease (ZFN)—mediated gene knockout of mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (Mgat1)

(2013) Natalie R. Sealover et al.   JOURNAL OF BIOTECHNOLOGY

The availability of glucose to CHO cells affects the intracellular lipid-linked oligosaccharide distribution, site occupancy and the N-glycosylation profile of a monoclonal antibody

(2013) Bo Liu et al.   JOURNAL OF BIOTECHNOLOGY

Engineering Hydrophobic Protein–Carbohydrate Interactions to Fine-Tune Monoclonal Antibodies

(2013) Xiaojie Yu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Glycoproteomic Analysis of Antibodies

(2013) Gerhild Zauner et al.   MOLECULAR & CELLULAR PROTEOMICS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Characterization of Therapeutic Antibodies and Related Products

(2012) Alain Beck et al.   ANALYTICAL CHEMISTRY

Engineering novel Lec1 glycosylation mutants in CHO-DUKX cells: Molecular insights and effector modulation of N-acetylglucosaminyltransferase I

(2012) Xiaotian Zhong et al.   BIOTECHNOLOGY AND BIOENGINEERING

In vivo cleavage of transgene donors promotes nuclease-mediated targeted integration

(2012) Sandra Cristea et al.   BIOTECHNOLOGY AND BIOENGINEERING

Monoclonal antibody therapeutics: history and future

(2012) Nicholas APS Buss et al.   CURRENT OPINION IN PHARMACOLOGY

IVIg-mediated amelioration of ITP in mice is dependent on sialic acid and SIGNR1

(2012) Inessa Schwab et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

Galactosylation variations in marketed therapeutic antibodies

(2012) T. Shantha Raju et al.   mAbs

Production, characterization and pharmacokinetic properties of antibodies with N-linked Mannose-5 glycans

(2012) Marcella Yu et al.   mAbs

Analysis and Functional Consequences of Increased Fab-Sialylation of Intravenous Immunoglobulin (IVIG) after Lectin Fractionation

(2012) Fabian Käsermann et al.   PLoS One

The role of sialic acid as a modulator of the anti-inflammatory activity of IgG

(2012) Sybille Böhm et al.   Seminars in Immunopathology

Enhancement of sialylation on humanized IgG-like bispecific antibody by overexpression of α2,6-sialyltransferase derived from Chinese hamster ovary cells

(2011) Masayoshi Onitsuka et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose

(2011) Michael J. Gramer et al.   BIOTECHNOLOGY AND BIOENGINEERING

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

Highly efficient deletion of FUT8 in CHO cell lines using zinc-finger nucleases yields cells that produce completely nonfucosylated antibodies

(2010) Laetitia Malphettes et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase

(2010) H. H. von Horsten et al.   GLYCOBIOLOGY

Analytical and Functional Aspects of Antibody Sialylation

(2010) Johannes Stadlmann et al.   JOURNAL OF CLINICAL IMMUNOLOGY

A Novel Role for the IgG Fc Glycan: The Anti-inflammatory Activity of Sialylated IgG Fcs

(2010) Robert M. Anthony et al.   JOURNAL OF CLINICAL IMMUNOLOGY

Production of therapeutic antibodies with controlled fucosylation

(2010) Naoko Yamane-Ohnuki et al.   mAbs

Engineering host cell lines to reduce terminal sialylation of secreted antibodies

(2010) Michael F. Naso et al.   mAbs

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Glycosylation as a strategy to improve antibody-based therapeutics

(2009) Roy Jefferis   NATURE REVIEWS DRUG DISCOVERY

A close look at human IgG sialylation and subclass distribution after lectin fractionation

(2009) Johannes Stadlmann et al.   PROTEOMICS

Antibody fucosylation differentially impacts cytotoxicity mediated by NK and PMN effector cells

(2008) M. Peipp et al.   BLOOD

Terminal sugars of Fc glycans influence antibody effector functions of IgGs

(2008) T Shantha Raju   CURRENT OPINION IN IMMUNOLOGY

Decrease in antithrombin III fucosylation by expressing GDP-fucose transporter siRNA in Chinese hamster ovary cells

(2008) Takeshi Omasa et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Establishment of HIV-1 resistance in CD4+ T cells by genome editing using zinc-finger nucleases

(2008) Elena E Perez et al.   NATURE BIOTECHNOLOGY

Recapitulation of IVIG Anti-Inflammatory Activity with a Recombinant IgG Fc

(2008) R. M. Anthony et al.   SCIENCE