Cryo-EM structure determination of small proteins by nanobody-binding scaffolds (Legobodies)

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM

(2021) Tomasz Uchański et al.   NATURE METHODS

Structure and mechanism of the ER-based glucosyltransferase ALG6

(2020) Joël S. Bloch et al.   NATURE

SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor

(2020) Markus Hoffmann et al.   CELL

Structure of human Frizzled5 by fiducial-assisted cryo-EM supports a heterodimeric mechanism of canonical Wnt signaling

(2020) Naotaka Tsutsumi et al.   eLife

Slaying SARS-CoV-2 One (Single-domain) Antibody at a Time

(2020) Timothy F. Czajka et al.   TRENDS IN MICROBIOLOGY

High-resolution structure determination of sub-100 kDa complexes using conventional cryo-EM

(2019) Mark A. Herzik et al.   Nature Communications

Structural basis for pH-dependent retrieval of ER proteins from the Golgi by the KDEL receptor

(2019) Philipp Bräuer et al.   SCIENCE

Fusion of DARPin to Aldolase Enables Visualization of Small Protein by Cryo-EM

(2019) Qing Yao et al.   STRUCTURE

A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold

(2019) Yuxi Liu et al.   Nature Communications

Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc

(2019) Yongchan Lee et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

(2019) Xiao Fan et al.   Nature Communications

Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix

(2019) Dorothee Liebschner et al.   Acta Crystallographica Section D-Structural Biology

Structure and drug resistance of the Plasmodium falciparum transporter PfCRT

(2019) Jonathan Kim et al.   NATURE

The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications

(2019) Hansjörg Götzke et al.   Nature Communications

LDAF1 and Seipin Form a Lipid Droplet Assembly Complex

(2019) Jeeyun Chung et al.   DEVELOPMENTAL CELL

High-yield monolayer graphene grids for near-atomic resolution cryoelectron microscopy

(2019) Yimo Han et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Yeast surface display platform for rapid discovery of conformationally selective nanobodies

(2018) Conor McMahon et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Synthetic single domain antibodies for the conformational trapping of membrane proteins

(2018) Iwan Zimmermann et al.   eLife

Structure of the post-translational protein translocation machinery of the ER membrane

(2018) Xudong Wu et al.   NATURE

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Addressing preferred specimen orientation in single-particle cryo-EM through tilting

(2017) Yong Zi Tan et al.   NATURE METHODS

Construction of novel repeat proteins with rigid and predictable structures using a shared helix method

(2017) Suk-Jun Youn et al.   Scientific Reports

Crystal structures of MBP fusion proteins

(2016) David S. Waugh   PROTEIN SCIENCE

Engineered high-affinity nanobodies recognizing staphylococcal Protein A and suitable for native isolation of protein complexes

(2015) Peter C. Fridy et al.   ANALYTICAL BIOCHEMISTRY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

How cryo-EM is revolutionizing structural biology

(2015) Xiao-chen Bai et al.   TRENDS IN BIOCHEMICAL SCIENCES

A Structurally Distinct Human Mycoplasma Protein that Generically Blocks Antigen-Antibody Union

(2014) R. K. Grover et al.   SCIENCE

Fabs Enable Single Particle cryoEM Studies of Small Proteins

(2012) Shenping Wu et al.   STRUCTURE