HDX-MS and MD Simulations Provide Evidence for Stabilization of the IgG1—FcγRIa (CD64a) Immune Complex Through Intermolecular Glycoprotein Bonds

Antibodies to watch in 2021

(2021) Hélène Kaplon et al.   mAbs

Dataset from HDX-MS Studies of IgG1 Glycoforms and Their Interactions with the FcγRIa (CD64) Receptor

(2021) Kyle W. Anderson et al.   JOURNAL OF RESEARCH OF THE NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY

Atomic Model Structure of the NIST Monoclonal Antibody (NISTmAb) Reference Material

(2021) Christina Bergonzo et al.   JOURNAL OF RESEARCH OF THE NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY

Reconciling Simulated Ensembles of Apomyoglobin with Experimental Hydrogen/Deuterium Exchange Data Using Bayesian Inference and Multiensemble Markov State Models

(2020) Hongbin Wan et al.   Journal of Chemical Theory and Computation

Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761

(2020) Kate Groves et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Glycosylation of Fcγ receptors influences their interaction with various IgG1 glycoforms

(2020) Florian Cambay et al.   MOLECULAR IMMUNOLOGY

A protocol for preparing explicitly solvated systems for stable molecular dynamics simulations

(2020) Daniel R. Roe et al.   JOURNAL OF CHEMICAL PHYSICS

SARS-CoV-2 Neutralizing Antibody LY-CoV555 in Outpatients with Covid-19

(2020) Peter Chen et al.   NEW ENGLAND JOURNAL OF MEDICINE

Interlaboratory Comparison of Hydrogen–Deuterium Exchange Mass Spectrometry Measurements of the Fab Fragment of NISTmAb

(2019) Jeffrey W. Hudgens et al.   ANALYTICAL CHEMISTRY

Reliable identification of significant differences in differential HX-MS measurements using a hybrid significance testing approach

(2019) Tyler S Hageman et al.   ANALYTICAL CHEMISTRY

A structural variant approach for establishing a detection limit in differential HX-MS measurements

(2019) Tyler S Hageman et al.   ANALYTICAL CHEMISTRY

Effects of terminal galactose residues in mannose α1-6 arm of Fc-glycan on the effector functions of therapeutic monoclonal antibodies

(2019) Michihiko Aoyama et al.   mAbs

Osteoporosis: Current and emerging therapies targeted to immunological checkpoints

(2019) Massimo De Martinis et al.   CURRENT MEDICINAL CHEMISTRY

Recommendations for performing, interpreting and reporting hydrogen deuterium exchange mass spectrometry (HDX-MS) experiments

(2019) Glenn R. Masson et al.   NATURE METHODS

Single-Molecule 3D Images of “Hole-Hole” IgG1 Homodimers by Individual-Particle Electron Tomography

(2019) Dongsheng Lei et al.   Scientific Reports

Impact of Fc N-glycan sialylation on IgG structure

(2019) Zhongqi Zhang et al.   mAbs

Effects of sialic acid linkage on antibody-fragment crystallizable receptor binding and antibody dependent cytotoxicity depend on levels of fucosylation/bisecting

(2019) Marco Thomann et al.   Bioanalysis

Monoclonal antibodies for the prevention of migraine

(2019) Bianca Raffaelli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Integrating hydrogen–deuterium exchange mass spectrometry with molecular dynamics simulations to probe lipid-modulated conformational changes in membrane proteins

(2019) Chloe Martens et al.   Nature Protocols

The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III

(2019) Rina Yogo et al.   Scientific Reports

Secukinumab may be a valid treatment option in patients with CNS demyelination and concurrent ankylosing spondylitis: Report of two clinical cases

(2019) Antonio Cortese et al.   Multiple Sclerosis and Related Disorders

Impact of Glycosylation on the Local Backbone Flexibility of Well-Defined IgG1-Fc Glycoforms Using Hydrogen Exchange-Mass Spectrometry

(2018) Apurva S. More et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

SWISS-MODEL: homology modelling of protein structures and complexes

(2018) Andrew Waterhouse et al.   NUCLEIC ACIDS RESEARCH

Progress and Challenges in the Design and Clinical Development of Antibodies for Cancer Therapy

(2018) Juan C. Almagro et al.   Frontiers in Immunology

Comment on Houde, D.; Berkowitz, S. A.; Engen, J. R., The Utility of Hydrogen/Deuterium Exchange Mass Spectrometry in Biopharmaceutical Comparability Studies. J. Pharm. Sci. 2011, 100, 2071-2086.

(2018) David D. Weis   JOURNAL OF PHARMACEUTICAL SCIENCES

Influence of N-glycosylation on effector functions and thermal stability of glycoengineered IgG1 monoclonal antibody with homogeneous glycoforms

(2018) Ryuta Wada et al.   mAbs

A Two-pronged Binding Mechanism of IgG to the Neonatal Fc Receptor Controls Complex Stability and IgG Serum Half-life

(2017) Pernille Foged Jensen et al.   MOLECULAR & CELLULAR PROTEOMICS

Identification of Fc Gamma Receptor Glycoforms That Produce Differential Binding Kinetics for Rituximab

(2017) Jerrard M. Hayes et al.   MOLECULAR & CELLULAR PROTEOMICS

Solid-Phase Enzymatic Remodeling Produces High Yields of Single Glycoform Antibodies

(2017) Venkata S. Tayi et al.   Biotechnology Journal

Effect of Fc-Glycan Structure on the Conformational Stability of IgG Revealed by Hydrogen/Deuterium Exchange and Limited Proteolysis

(2016) Jing Fang et al.   BIOCHEMISTRY

Structural mechanism of high affinity FcγRI recognition of immunoglobulin G

(2015) Jinghua Lu et al.   IMMUNOLOGICAL REVIEWS

ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB

(2015) James A. Maier et al.   Journal of Chemical Theory and Computation

Hydrogen-Deuterium Exchange Mass Spectrometry as an Emerging Analytical Tool for Stabilization and Formulation Development of Therapeutic Monoclonal Antibodies

(2015) Ranajoy Majumdar et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Structure of FcγRI in complex with Fc reveals the importance of glycan recognition for high-affinity IgG binding

(2015) Jinghua Lu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for binding of human IgG1 to its high-affinity human receptor FcγRI

(2015) Masato Kiyoshi et al.   Nature Communications

Structural insights into the interaction of human IgG1 with FcγRI: no direct role of glycans in binding

(2015) Vaheh Oganesyan et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Multi-Angle Effector Function Analysis of Human Monoclonal IgG Glycovariants

(2015) Tetyana Dashivets et al.   PLoS One

Effects of Protein–Ligand Interactions on Hydrogen/Deuterium Exchange Kinetics: Canonical and Noncanonical Scenarios

(2014) Modupeola A. Sowole et al.   ANALYTICAL CHEMISTRY

Fc Gamma Receptor Glycosylation Modulates the Binding of IgG Glycoforms: A Requirement for Stable Antibody Interactions

(2014) Jerrard M. Hayes et al.   JOURNAL OF PROTEOME RESEARCH

Investigating the Interaction between the Neonatal Fc Receptor and Monoclonal Antibody Variants by Hydrogen/Deuterium Exchange Mass Spectrometry

(2014) Pernille Foged Jensen et al.   MOLECULAR & CELLULAR PROTEOMICS

The high-affinity human IgG receptor Fc RI (CD64) promotes IgG-mediated inflammation, anaphylaxis, and antitumor immunotherapy

(2013) D. A. Mancardi et al.   BLOOD

Current evaluation of alemtuzumab in multiple sclerosis

(2013) Patricia Katherine Coyle   EXPERT OPINION ON BIOLOGICAL THERAPY

PTRAJ and CPPTRAJ: Software for Processing and Analysis of Molecular Dynamics Trajectory Data

(2013) Daniel R. Roe et al.   Journal of Chemical Theory and Computation

Routine Microsecond Molecular Dynamics Simulations with AMBER on GPUs. 2. Explicit Solvent Particle Mesh Ewald

(2013) Romelia Salomon-Ferrer et al.   Journal of Chemical Theory and Computation

N-Linked Glycan Structures of the Human Fcγ Receptors Produced in NS0 Cells

(2013) Eoin F. J. Cosgrave et al.   JOURNAL OF PROTEOME RESEARCH

Consequences of glycan truncation on Fc structural integrity

(2013) Patrick M Buck et al.   mAbs

The binding of soluble recombinant human Fcγ receptor I for human immunoglobulin G is conferred by its first and second extracellular domains

(2013) Yoshiharu Asaoka et al.   MOLECULAR IMMUNOLOGY

Chemical and Structural Analysis of an Antibody Folding Intermediate Trapped during Glycan Biosynthesis

(2012) Thomas A. Bowden et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

HDX Workbench: Software for the Analysis of H/D Exchange MS Data

(2012) Bruce D. Pascal et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Increased serum clearance of oligomannose species present on a human IgG1 molecule

(2012) Leslie Alessandri et al.   mAbs

Platform Dependencies in Bottom-up Hydrogen/Deuterium Exchange Mass Spectrometry

(2012) Kyle M. Burns et al.   MOLECULAR & CELLULAR PROTEOMICS

High-mannose glycans on the Fc region of therapeutic IgG antibodies increase serum clearance in humans

(2011) A. M. Goetze et al.   GLYCOBIOLOGY

Crystal Structure of Fcγ Receptor I and Its Implication in High Affinity γ-Immunoglobulin Binding

(2011) Jinghua Lu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

NMR analysis demonstrates immunoglobulin G N-glycans are accessible and dynamic

(2011) Adam W Barb et al.   Nature Chemical Biology

Therapeutic antibodies in ophthalmology

(2010) Charlotte Magdelaine-Beuzelin et al.   mAbs

Post-translational Modifications Differentially Affect IgG1 Conformation and Receptor Binding

(2010) Damian Houde et al.   MOLECULAR & CELLULAR PROTEOMICS

Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

(2009) Damian Houde et al.   ANALYTICAL CHEMISTRY

Branch-Specific Sialylation of IgG-Fc Glycans by ST6Gal-I

(2009) Adam W. Barb et al.   BIOCHEMISTRY

Terminal sugars of Fc glycans influence antibody effector functions of IgGs

(2008) T Shantha Raju   CURRENT OPINION IN IMMUNOLOGY

Determination of Alkali and Halide Monovalent Ion Parameters for Use in Explicitly Solvated Biomolecular Simulations

(2008) In Suk Joung et al.   JOURNAL OF PHYSICAL CHEMISTRY B