Decoupling Growth and Protein Production in CHO Cells: A Targeted Approach

Light-mediated control of Gene expression in mammalian cells

(2020) Mayumi Yamada et al.   NEUROSCIENCE RESEARCH

Bacteriophage Inspired Growth-Decoupled Recombinant Protein Production in Escherichia coli

(2020) Patrick Stargardt et al.   ACS Synthetic Biology

Reduced Apoptosis in Chinese Hamster Ovary Cells via Optimized CRISPR Interference

(2019) Kai Xiong et al.   BIOTECHNOLOGY AND BIOENGINEERING

Limiting the metabolic burden of recombinant protein expression during selection yields pools with higher expression levels

(2019) E‐Ching Ong et al.   BIOTECHNOLOGY PROGRESS

Reducing recombinant protein expression during CHO pool selection enhances frequency of high-producing cells

(2019) Adeline Poulain et al.   JOURNAL OF BIOTECHNOLOGY

Model-based optimization of temperature and pH shift to increase volumetric productivity of a Chinese hamster ovary fed-batch process

(2019) Katrin Paul et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

How to Choose the Right Inducible Gene Expression System for Mammalian Studies?

(2019) Kallunki et al.   Cells

Awakening dormant glycosyltransferases in CHO cells with CRISPRa

(2019) Karen Julie la Cour Karottki et al.   BIOTECHNOLOGY AND BIOENGINEERING

Kinase-independent function of E-type cyclins in liver cancer

(2018) Yan Geng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

(2018) Arun K. Dangi et al.   Frontiers in Pharmacology

Systematic development of temperature shift strategies for Chinese hamster ovary cells based on short duration cultures and kinetic modeling

(2018) Jianlin Xu et al.   mAbs

Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells

(2018) Julia Baaske et al.   Scientific Reports

A Post-translational Metabolic Switch Enables Complete Decoupling of Bacterial Growth from Biopolymer Production in Engineered Escherichia coli

(2018) Gonzalo Durante-Rodríguez et al.   ACS Synthetic Biology

Purity by design: Reducing impurities in bioproduction by stimulus-controlled global translational downregulation of non-product proteins

(2018) Daniel Bojar et al.   METABOLIC ENGINEERING

Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch

(2017) Adeline Poulain et al.   JOURNAL OF BIOTECHNOLOGY

Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors

(2017) Dirk A. Kleinjan et al.   Nature Communications

Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion

(2017) Thomas Beuchert Kallehauge et al.   Scientific Reports

Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering

(2017) Tuhin Kumar Guha et al.   Computational and Structural Biotechnology Journal

Enhanced protein and biochemical production using CRISPRi-based growth switches

(2016) Songyuan Li et al.   METABOLIC ENGINEERING

Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors

(2016) Toyoaki Natsume et al.   Cell Reports

Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using Recombinant Human Tissue Plasminogen Activator Production Culture

(2016) Andrea Bedoya-López et al.   PLoS One

Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR

(2015) Michael Boettcher et al.   MOLECULAR CELL

A synthetic growth switch based on controlled expression of RNA polymerase

(2015) J. Izard et al.   Molecular Systems Biology

Highly efficient Cas9-mediated transcriptional programming

(2015) Alejandro Chavez et al.   NATURE METHODS

Effect of sodium butyrate on the assembly, charge variants, and galactosylation of antibody produced in recombinant Chinese hamster ovary cells

(2014) Jong Kwang Hong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

(2014) Zhimei Du et al.   BIOTECHNOLOGY AND BIOENGINEERING

It's time to regulate: Coping with product-induced nongenetic clonal instability in CHO cell lines via regulated protein expression

(2014) Shahram Misaghi et al.   BIOTECHNOLOGY PROGRESS

The   isoform of topoisomerase II is required for hypercompaction of mitotic chromosomes in human cells

(2014) C. J. Farr et al.   NUCLEIC ACIDS RESEARCH

ATR (ataxia telangiectasia mutated- and Rad3-related kinase) is activated by mild hypothermia in mammalian cells and subsequently activates p53

(2011) Anne Roobol et al.   BIOCHEMICAL JOURNAL

Strategies for the enhancement of recombinant protein production from mammalian cells by growth arrest

(2010) Kevin Sunley et al.   BIOTECHNOLOGY ADVANCES

The effects of culture conditions on the glycosylation of secreted human placental alkaline phosphatase produced in Chinese hamster ovary cells

(2008) Jong Hyun Nam et al.   BIOTECHNOLOGY AND BIOENGINEERING

Metabolic Rates, Growth Phase, and mRNA Levels Influence Cell-Specific Antibody Production Levels from In Vitro-Cultured Mammalian Cells at Sub-Physiological Temperatures

(2008) Rosalyn J. Marchant et al.   MOLECULAR BIOTECHNOLOGY