Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

Vector and Cell Line Engineering Technologies Toward Recombinant Protein Expression in Mammalian Cell Lines

(2018) Seyedeh Hoda Jazayeri et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhanced production of anti-PD1 antibody in CHO cells through transient co-transfection with anti-apoptotic genes Bcl-xL and Mcl-1

(2018) Xinyu Zhang et al.   BIOPROCESS AND BIOSYSTEMS ENGINEERING

Inhibition of apoptosis using exosomes in Chinese hamster ovary cell culture

(2018) Seora Han et al.   BIOTECHNOLOGY AND BIOENGINEERING

Targeted knock-in of CreERT2 in zebrafish using CRISPR/Cas9

(2018) Gokul Kesavan et al.   CELL AND TISSUE RESEARCH

CRISPR-cas System as a Genome Engineering Platform: Applications in Biomedicine and Biotechnology

(2018) Atieh Hashemi   CURRENT GENE THERAPY

Monoclonal Antibodies for Emerging Infectious Diseases — Borrowing from History

(2018) Hilary D. Marston et al.   NEW ENGLAND JOURNAL OF MEDICINE

CRISPR-Cas Targeting of Host Genes as an Antiviral Strategy

(2018) Shuliang Chen et al.   Viruses-Basel

Applications of CRISPR genome editing technology in drug target identification and validation

(2017) Quinn Lu et al.   Expert Opinion on Drug Discovery

Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm

(2017) Lisa C. Shriver-Lake et al.   JOURNAL OF IMMUNOLOGICAL METHODS

Development of a pre-glycoengineered CHO-K1 host cell line for the expression of antibodies with enhanced Fc mediated effector function

(2017) Oliver Popp et al.   mAbs

Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa

(2017) Céline Morineau et al.   PLANT BIOTECHNOLOGY JOURNAL

Deletion of a target gene in Indica rice via CRISPR/Cas9

(2017) Ying Wang et al.   PLANT CELL REPORTS

Post-translational Modification of Caspases: The Other Side of Apoptosis Regulation

(2017) Alexey V. Zamaraev et al.   TRENDS IN CELL BIOLOGY

Monoclonal antibodies: A review of therapeutic applications and future prospects

(2017) Aliyu Mahmuda et al.   TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

Therapeutic genome engineering via CRISPR-Cas systems

(2017) Ana M. Moreno et al.   Wiley Interdisciplinary Reviews-Systems Biology and Medicine

Muscle-specific CRISPR/Cas9 dystrophin gene editing ameliorates pathophysiology in a mouse model for Duchenne muscular dystrophy

(2017) Niclas E. Bengtsson et al.   Nature Communications

Engineering cell sensing and responses using a GPCR-coupled CRISPR-Cas system

(2017) Nathan H. Kipniss et al.   Nature Communications

Advancements in Developing Strategies for Sterilizing and Functional HIV Cures

(2017) Wei Xu et al.   Biomed Research International

Over-expression of a Codon Optimized Yeast Cytosolic Pyruvate Carboxylase (PYC2) in CHO Cells for an Augmented Lactate Metabolism

(2017) Sanjeev K. Gupta et al.   Frontiers in Pharmacology

Mammalian Systems Biotechnology Reveals Global Cellular Adaptations in a Recombinant CHO Cell Line

(2017) Faraaz Noor Khan Yusufi et al.   Cell Systems

In situ activation of platelets with checkpoint inhibitors for post-surgical cancer immunotherapy

(2017) Chao Wang et al.   Nature Biomedical Engineering

Metabolic engineering of CHO cells for the development of a robust protein production platform

(2017) Sanjeev Kumar Gupta et al.   PLoS One

Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9

(2017) Risa Ueta et al.   Scientific Reports

An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells

(2017) Yifang Xie et al.   Scientific Reports

Synergistic Transcutaneous Immunotherapy Enhances Antitumor Immune Responses through Delivery of Checkpoint Inhibitors

(2016) Yanqi Ye et al.   ACS Nano

CRISPR/Cas9 for Human Genome Engineering and Disease Research

(2016) Xin Xiong et al.   Annual Review of Genomics and Human Genetics

Reduction of ammonia and lactate through the coupling of glutamine synthetase selection and downregulation of lactate dehydrogenase-A in CHO cells

(2016) Soo Min Noh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Advances in recombinant antibody manufacturing

(2016) Renate Kunert et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement

(2016) Evan Wells et al.   Biotechnology Journal

Gene editing for cell engineering: trends and applications

(2016) Sanjeev K. Gupta et al.   CRITICAL REVIEWS IN BIOTECHNOLOGY

Microbial platform technology for recombinant antibody fragment production: A review

(2016) Sanjeev Kumar Gupta et al.   CRITICAL REVIEWS IN MICROBIOLOGY

Deletion of the GAA repeats from the human frataxin gene using the CRISPR-Cas9 system in YG8R-derived cells and mouse models of Friedreich ataxia

(2016) D L Ouellet et al.   GENE THERAPY

Enhanced Cancer Immunotherapy by Microneedle Patch-Assisted Delivery of Anti-PD1 Antibody

(2016) Chao Wang et al.   NANO LETTERS

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Cornerstones of CRISPR–Cas in drug discovery and therapy

(2016) Christof Fellmann et al.   NATURE REVIEWS DRUG DISCOVERY

ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under field drought stress conditions

(2016) Jinrui Shi et al.   PLANT BIOTECHNOLOGY JOURNAL

Cellular engineering for therapeutic protein production: product quality, host modification, and process improvement

(2016) Evan Wells et al.   Biotechnology Journal

The art of CHO cell engineering: A comprehensive retrospect and future perspectives

(2015) Simon Fischer et al.   BIOTECHNOLOGY ADVANCES

miR-2861 as novel HDAC5 inhibitor in CHO cells enhances productivity while maintaining product quality

(2015) Simon Fischer et al.   BIOTECHNOLOGY AND BIOENGINEERING

Integrated cell and process engineering for improved transient production of a “difficult-to-express“ fusion protein by CHO cells

(2015) Yusuf B. Johari et al.   BIOTECHNOLOGY AND BIOENGINEERING

Designing cell lines for viral vaccine production: Where do we stand?

(2015) Yvonne Genzel   Biotechnology Journal

Chemical manipulation of the mTORC1 pathway in industrially relevant CHOK1 cells enhances production of therapeutic proteins

(2015) Nazanin Dadehbeigi et al.   Biotechnology Journal

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

(2015) Kah Fai Chan et al.   Biotechnology Journal

Harnessing the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated Cas9 system to disrupt the hepatitis B virus

(2015) S Zhen et al.   GENE THERAPY

CRISPR/Cas9-mediatedDax1knockout in the monkey recapitulates human AHC-HH

(2015) Yu Kang et al.   HUMAN MOLECULAR GENETICS

CRISPR/Cas9: a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases

(2015) Zhuchi Tu et al.   Molecular Neurodegeneration

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Use of the CRISPR/Cas9 system as an intracellular defense against HIV-1 infection in human cells

(2015) Hsin-Kai Liao et al.   Nature Communications

Designing cell lines for viral vaccine production: Where do we stand?

(2015) Yvonne Genzel   Biotechnology Journal

Chemical manipulation of the mTORC1 pathway in industrially relevant CHOK1 cells enhances production of therapeutic proteins

(2015) Nazanin Dadehbeigi et al.   Biotechnology Journal

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies

(2015) Kah Fai Chan et al.   Biotechnology Journal

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9

(2015) Hongmei Lisa Li et al.   Stem Cell Reports

Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9

(2015) Luqing Zhang et al.   PLoS One

Use of a small molecule cell cycle inhibitor to control cell growth and improve specific productivity and product quality of recombinant proteins in CHO cell cultures

(2014) Zhimei Du et al.   BIOTECHNOLOGY AND BIOENGINEERING

Overexpression of microRNAs enhances recombinant protein production in Chinese hamster ovary cells

(2014) Wan Ping Loh et al.   Biotechnology Journal

Enhanced transient recombinant protein production in CHO cells through the co-transfection of the product gene withBcl-xL

(2014) Matthew P. Zustiak et al.   Biotechnology Journal

A functional high-content miRNA screen identifies miR-30 family to boost recombinant protein production in CHO cells

(2014) Simon Fischer et al.   Biotechnology Journal

Conserved microRNA function as a basis for Chinese hamster ovary cell engineering

(2014) Paul S. Kelly et al.   BIOTECHNOLOGY LETTERS

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

(2014) Randall J. Platt et al.   CELL

Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells

(2014) Yuxuan Wu et al.   CELL RESEARCH

Engineering cells to improve protein expression

(2014) Su Xiao et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Stable overexpression of miR-17 enhances recombinant protein production of CHO cells

(2014) Vaibhav Jadhav et al.   JOURNAL OF BIOTECHNOLOGY

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

(2014) Alexandra E. Briner et al.   MOLECULAR CELL

CRISPR-mediated direct mutation of cancer genes in the mouse liver

(2014) Wen Xue et al.   NATURE

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection

(2014) W. Hu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Prevention of muscular dystrophy in mice by CRISPR/Cas9-mediated editing of germline DNA

(2014) C. Long et al.   SCIENCE

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

CHO microRNA engineering is growing up: Recent successes and future challenges

(2013) Vaibhav Jadhav et al.   BIOTECHNOLOGY ADVANCES

Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells

(2013) Leon P. Pybus et al.   BIOTECHNOLOGY AND BIOENGINEERING

CHO cell culture longevity and recombinant protein yield are enhanced by depletion of miR-7 activity via sponge decoy vectors

(2013) Noelia Sanchez et al.   Biotechnology Journal

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9

(2013) Yuxuan Wu et al.   Cell Stem Cell

TALEN-induced gene knock out inDrosophila

(2013) Takefumi Kondo et al.   DEVELOPMENT GROWTH & DIFFERENTIATION

Exploiting the metabolism of PYC expressing HEK293 cells in fed-batch cultures

(2013) Cédric Vallée et al.   JOURNAL OF BIOTECHNOLOGY

Stable inhibition of mmu-miR-466h-5p improves apoptosis resistance and protein production in CHO cells

(2013) Aliaksandr Druz et al.   METABOLIC ENGINEERING

Stable microRNA expression enhances therapeutic antibody productivity of Chinese hamster ovary cells

(2013) Michaela Strotbek et al.   METABOLIC ENGINEERING

A bacteriophage encodes its own CRISPR/Cas adaptive response to evade host innate immunity

(2013) Kimberley D. Seed et al.   NATURE

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

Structural basis for diverse N-glycan recognition by HIV-1–neutralizing V1–V2–directed antibody PG16

(2013) Marie Pancera et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

TALEN-Based Gene Disruption in the Dengue Vector Aedes aegypti

(2013) Azadeh Aryan et al.   PLoS One

Overexpression of CHOP alone and in combination with chaperones is effective in improving antibody production in mammalian cells

(2012) Daisuke Nishimiya et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

A screening method to assess biological effects of microRNA overexpression in Chinese hamster ovary cells

(2012) Vaibhav Jadhav et al.   BIOTECHNOLOGY AND BIOENGINEERING

Impact of miR-7 over-expression on the proteome of Chinese hamster ovary cells

(2012) Paula Meleady et al.   JOURNAL OF BIOTECHNOLOGY

Quantitative evaluation of fucose reducing effects in a humanized antibody on Fcγ receptor binding and antibody-dependent cell-mediated cytotoxicity activities

(2012) Shan Chung et al.   mAbs

High-efficiency TALEN-based gene editing produces disease-resistant rice

(2012) Ting Li et al.   NATURE BIOTECHNOLOGY

miRNAs – pathway engineering of CHO cell factories that avoids translational burdening

(2012) Matthias Hackl et al.   TRENDS IN BIOTECHNOLOGY

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Mammalian cell protein expression for biopharmaceutical production

(2011) Jianwei Zhu   BIOTECHNOLOGY ADVANCES

Genome Engineering With Zinc-Finger Nucleases

(2011) Dana Carroll   GENETICS

Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

(2011) Meixia Zhou et al.   JOURNAL OF BIOTECHNOLOGY

Enhanced glycoprotein production in HEK-293 cells expressing pyruvate carboxylase

(2011) Olivier Henry et al.   METABOLIC ENGINEERING

Targeted gene disruption in somatic zebrafish cells using engineered TALENs

(2011) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Primer: genome editing with engineered nucleases

(2011) Natalie de Souza   NATURE METHODS

Ectopic expression of human mTOR increases viability, robustness, cell size, proliferation, and antibody production of chinese hamster ovary cells

(2010) Imke A.J. Dreesen et al.   BIOTECHNOLOGY AND BIOENGINEERING

Targeting DNA Double-Strand Breaks with TAL Effector Nucleases

(2010) M. Christian et al.   GENETICS

Engineering CHO cell growth and recombinant protein productivity by overexpression of miR-7

(2010) N. Barron et al.   JOURNAL OF BIOTECHNOLOGY

Cell culture processes for monoclonal antibody production

(2010) Feng Li et al.   mAbs

Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)

(2010) Jeffry D Sander et al.   NATURE METHODS

Guidelines to cell engineering for monoclonal antibody production

(2009) A. Rita Costa et al.   EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

Overexpression of cold-inducible RNA-binding protein increases interferon-γ production in Chinese-hamster ovary cells

(2008) Hong Kiat Tan et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Assessment of cell engineering strategies for improved therapeutic protein production in CHO cells

(2008) Chaya Mohan et al.   Biotechnology Journal

Trends in Glycosylation, Glycoanalysis and Glycoengineering of Therapeutic Antibodies and Fc-Fusion Proteins

(2008) Alain Beck et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Effect of Increased Expression of Protein Disulfide Isomerase and Heavy Chain Binding Protein on Antibody Secretion in a Recombinant CHO Cell Line

(2005) Nicole Borth et al.   BIOTECHNOLOGY PROGRESS