Quantitative live-cell imaging and computational modeling shed new light on endogenous WNT/CTNNB1 signaling dynamics

Wnt regulation: exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex

(2020) Kristina N. Schaefer et al.   MOLECULAR BIOLOGY OF THE CELL

Limited dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction

(2020) Wei Kan et al.   eLife

Measuring ligand-cell surface receptor affinities with axial line-scanning fluorescence correlation spectroscopy

(2020) Antonia Franziska Eckert et al.   eLife

β-catenin-mediated Wnt signal transduction proceeds through an endocytosis-independent mechanism

(2020) Ellen Youngsoo Rim et al.   MOLECULAR BIOLOGY OF THE CELL

Single-molecule dynamics of Dishevelled at the plasma membrane and Wnt pathway activation

(2020) Wenzhe Ma et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nuclear Regulation of Wnt/β-Catenin Signaling: It’s a Complex Situation

(2020) Christin C. Anthony et al.   Genes

Walking the tight wire between cell adhesion and WNT signalling: a balancing act for β-catenin

(2020) Tanne van der Wal et al.   Open Biology

WNT Activates the AAK1 Kinase to Promote Clathrin-Mediated Endocytosis of LRP6 and Establish a Negative Feedback Loop

(2019) Megan J. Agajanian et al.   Cell Reports

Synergy with TGFβ ligands switches WNT pathway dynamics from transient to sustained during human pluripotent cell differentiation

(2019) Joseph Massey et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Wnt/Beta-Catenin Signaling Regulation and a Role for Biomolecular Condensates

(2019) Kristina N. Schaefer et al.   DEVELOPMENTAL CELL

Destruction complex dynamics: Wnt/β-catenin signaling alters Axin-GSK3β interactions in vivo

(2019) Daniel B. Lybrand et al.   DEVELOPMENT

Absolute quantification of cohesin, CTCF and their regulators in human cells

(2019) Johann Holzmann et al.   eLife

Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids

(2019) Sapna Chhabra et al.   PLOS BIOLOGY

Multiprotein complexes governing Wnt signal transduction

(2018) Melissa Gammons et al.   CURRENT OPINION IN CELL BIOLOGY

Wnt signalling: conquering complexity

(2018) Katrin E. Wiese et al.   DEVELOPMENT

Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals

(2018) Kan Yaguchi et al.   JOURNAL OF CELL BIOLOGY

Supramolecular assembly of the beta-catenin destruction complex and the effect of Wnt signaling on its localization, molecular size, and activity in vivo

(2018) Kristina N. Schaefer et al.   PLoS Genetics

TCF/LEF dependent and independent transcriptional regulation of Wnt/β‐catenin target genes

(2018) Nikolaos Doumpas et al.   EMBO JOURNAL

Mutations in Bcl9 and Pygo genes cause congenital heart defects by tissue-specific perturbation of Wnt/β-catenin signaling

(2018) Claudio Cantù et al.   GENES & DEVELOPMENT

Determination of Rate-Limiting Factor for Formation of Beta-Catenin Destruction Complexes Using Absolute Protein Quantification

(2017) Masashi Kitazawa et al.   JOURNAL OF PROTEOME RESEARCH

Reconstituting regulation of the canonical Wnt pathway by engineering a minimal β-catenin destruction machine

(2017) Mira I. Pronobis et al.   MOLECULAR BIOLOGY OF THE CELL

Probing the canonicity of the Wnt/Wingless signaling pathway

(2017) Alexandra Franz et al.   PLoS Genetics

Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/BCL9

(2017) Laurens M van Tienen et al.   eLife

Generating Cellular Diversity and Spatial Form: Wnt Signaling and the Evolution of Multicellular Animals

(2016) Kyle M. Loh et al.   DEVELOPMENTAL CELL

mScarlet: a bright monomeric red fluorescent protein for cellular imaging

(2016) Daphne S Bindels et al.   NATURE METHODS

Wnt signaling in cancer

(2016) T Zhan et al.   ONCOGENE

Construction and Experimental Validation of a Petri Net Model of Wnt/β-Catenin Signaling

(2016) Annika Jacobsen et al.   PLoS One

Quantifying β-catenin subcellular dynamics and cyclin D1 mRNA transcription during Wnt signaling in single living cells

(2016) Pinhas Kafri et al.   eLife

Comparative genetic screens in human cells reveal new regulatory mechanisms in WNT signaling

(2016) Andres M Lebensohn et al.   eLife

Structure, Dynamics, and Functionality of Tankyrase Inhibitor-Induced Degradasomes

(2015) T. E. Thorvaldsen et al.   MOLECULAR CANCER RESEARCH

An ancient Pygo-dependent Wnt enhanceosome integrated by Chip/LDB-SSDP

(2015) Marc Fiedler et al.   eLife

A novel GSK3-regulated APC:Axin interaction regulates Wnt signaling by driving a catalytic cycle of efficient βcatenin destruction

(2015) Mira I Pronobis et al.   eLife

Analysis of Wnt signaling β-catenin spatial dynamics in HEK293T cells

(2014) Chin Tan et al.   BMC Systems Biology

Wnt-induced transcriptional activation is exclusively mediated by TCF/LEF

(2014) Jurian Schuijers et al.   EMBO JOURNAL

Recent applications of fluorescence correlation spectroscopy in live systems

(2014) Radek Macháň et al.   FEBS LETTERS

Testing Models of the APC Tumor Suppressor/β-Catenin Interaction Reshapes Our View of the Destruction Complex in Wnt Signaling

(2014) Robert J. Yamulla et al.   GENETICS

Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line

(2014) Patrick Essletzbichler et al.   GENOME RESEARCH

Nodal·Gdf1 Heterodimers with Bound Prodomains Enable Serum-independent Nodal Signaling and Endoderm Differentiation

(2014) Christophe Fuerer et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

In vivo analysis of formation and endocytosis of the Wnt/ -Catenin signaling complex in zebrafish embryos

(2014) A. I. H. Hagemann et al.   JOURNAL OF CELL SCIENCE

Comparative assessment of fluorescent transgene methods for quantitative imaging in human cells

(2014) Robert Mahen et al.   MOLECULAR BIOLOGY OF THE CELL

Self-association of the APC tumor suppressor is required for the assembly, stability, and activity of the Wnt signaling destruction complex

(2014) Ezgi Kunttas-Tatli et al.   MOLECULAR BIOLOGY OF THE CELL

Quantifying intracellular dynamics using fluorescence fluctuation spectroscopy

(2014) Mark A. Hink   PROTOPLASMA

Quantitative live-cell imaging reveals spatio-temporal dynamics and cytoplasmic assembly of the 26S proteasome

(2014) Chan-Gi Pack et al.   Nature Communications

Wnt signalling induces accumulation of phosphorylated  -catenin in two distinct cytosolic complexes

(2014) J. P. Gerlach et al.   Open Biology

Structural basis of GSK-3 inhibition by N-terminal phosphorylation and by the Wnt receptor LRP6

(2014) Jennifer L Stamos et al.   eLife

Quantitative Analysis of Self-Association and Mobility of Annexin A4 at the Plasma Membrane

(2013) Kevin C. Crosby et al.   BIOPHYSICAL JOURNAL

Analysing the impact of nucleo-cytoplasmic shuttling of β-catenin and its antagonists APC, Axin and GSK3 on Wnt/β-catenin signalling

(2013) Yvonne Schmitz et al.   CELLULAR SIGNALLING

The transience of transient overexpression

(2013) Toby J Gibson et al.   NATURE METHODS

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

Probing transcription factor diffusion dynamics in the living mammalian embryo with photoactivatable fluorescence correlation spectroscopy

(2013) Gurpreet Kaur et al.   Nature Communications

DNA-dependent Oct4–Sox2 interaction and diffusion properties characteristic of the pluripotent cell state revealed by fluorescence spectroscopy

(2012) Chen Sok Lam et al.   BIOCHEMICAL JOURNAL

Wnt Signaling through Inhibition of β-Catenin Degradation in an Intact Axin1 Complex

(2012) Vivian S.W. Li et al.   CELL

The many faces and functions of β-catenin

(2012) Tomas Valenta et al.   EMBO JOURNAL

Reversible Modification of Adenomatous Polyposis Coli (APC) with K63-linked Polyubiquitin Regulates the Assembly and Activity of the β-Catenin Destruction Complex

(2012) Hoanh Tran et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Wnt Signalling Pathway Parameters for Mammalian Cells

(2012) Chin Wee Tan et al.   PLoS One

Maternal Wnt/β-Catenin Signaling Coactivates Transcription through NF-κB Binding Sites during Xenopus Axis Formation

(2012) Neil J. Armstrong et al.   PLoS One

Kinetic Responses of  -Catenin Specify the Sites of Wnt Control

(2012) A. R. Hernandez et al.   SCIENCE

Celebrating 30 Years of Wnt Signaling

(2012) F. Verkaar et al.   Science Signaling

The Evolution of the Wnt Pathway

(2012) T. W. Holstein   Cold Spring Harbor Perspectives in Biology

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

Fluorescence Correlation Spectroscopy: Past, Present, Future

(2011) Elliot L. Elson   BIOPHYSICAL JOURNAL

The Ubiquitin-Specific Protease USP34 Regulates Axin Stability and Wnt/ -Catenin Signaling

(2011) T. T. H. Lui et al.   MOLECULAR AND CELLULAR BIOLOGY

Deconstructing the ßcatenin destruction complex: mechanistic roles for the tumor suppressor APC in regulating Wnt signaling

(2011) David M. Roberts et al.   MOLECULAR BIOLOGY OF THE CELL

Ebola virus entry requires the cholesterol transporter Niemann–Pick C1

(2011) Jan E. Carette et al.   NATURE

Dishevelled interacts with the DIX domain polymerization interface of Axin to interfere with its function in down-regulating β-catenin

(2011) Marc Fiedler et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulation of β-Catenin Nuclear Dynamics by GSK-3β Involves a LEF-1 Positive Feedback Loop

(2011) Cara Jamieson et al.   TRAFFIC

The Adenomatous polyposis coli tumour suppressor is essential for Axin complex assembly and function and opposes Axin's interaction with Dishevelled

(2011) C. Mendoza-Topaz et al.   Open Biology

Wnt Signaling Requires Sequestration of Glycogen Synthase Kinase 3 inside Multivesicular Endosomes

(2010) Vincent F. Taelman et al.   CELL

β-Catenin Phosphorylated at Serine 45 Is Spatially Uncoupled from β-Catenin Phosphorylated in the GSK3 Domain: Implications for Signaling

(2010) Meghan T. Maher et al.   PLoS One

Towards an integrated view of Wnt signaling in development

(2009) R. van Amerongen et al.   DEVELOPMENT

Interactions between SOX factors and Wnt/β-catenin signaling in development and disease

(2009) Jay D. Kormish et al.   DEVELOPMENTAL DYNAMICS

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Mapping eGFP Oligomer Mobility in Living Cell Nuclei

(2009) Nicolas Dross et al.   PLoS One

Rac1 Activation Controls Nuclear Localization of β-catenin during Canonical Wnt Signaling

(2008) Ximei Wu et al.   CELL

O-GlcNAc-glycosylation of β-catenin regulates its nuclear localization and transcriptional activity

(2008) Ria Sayat et al.   EXPERIMENTAL CELL RESEARCH

Functional definition of the mutation cluster region of adenomatous polyposis coli in colorectal tumours

(2008) E. M. Kohler et al.   HUMAN MOLECULAR GENETICS

Plasma membrane recruitment of dephosphorylated  -catenin upon activation of the Wnt pathway

(2008) J. Hendriksen et al.   JOURNAL OF CELL SCIENCE

Analyzing real-time PCR data by the comparative CT method

(2008) Thomas D Schmittgen et al.   Nature Protocols

Recruitment of adenomatous polyposis coli and β-catenin to axin-puncta

(2008) M C Faux et al.   ONCOGENE

Precise Measurement of Diffusion Coefficients using Scanning Fluorescence Correlation Spectroscopy

(2007) Zdeněk Petrášek et al.   BIOPHYSICAL JOURNAL

Mapping the Number of Molecules and Brightness in the Laser Scanning Microscope

(2007) Michelle A. Digman et al.   BIOPHYSICAL JOURNAL