Article
Biochemistry & Molecular Biology
Min Gyu Lee, Hwamok Oh, Jong Woo Park, Jueng Soo You, Jeung-Whan Han
Summary: Nuclear S6K1 functions in the regulation of Wnt target gene expression and has an impact on cell proliferation and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Jingjing Wang, Xiaoqiang Zheng, Xiao Fu, Aimin Jiang, Yu Yao, Wangxiao He
Summary: This article introduces a new method for the treatment of metastatic melanoma by using a bifunctional supramolecular peptide HBBplus@CA, which simultaneously inhibits both the Wnt/β-catenin and MAPK/ERK signaling pathways. It effectively suppresses melanoma cell proliferation, migration, and invasion, and inhibits lung metastasis and prolongs survival in a mouse model.
Article
Biochemistry & Molecular Biology
Irina Giralt, Gabriel Gallo-Oller, Natalia Navarro, Patricia Zarzosa, Guillem Pons, Ainara Magdaleno, Miguel F. Segura, Constantino Sabado, Raquel Hladun, Diego Arango, Jose Sanchez de Toledo, Lucas Moreno, Soledad Gallego, Josep Roma
Summary: The study demonstrates the therapeutic potential of DKK-1 pharmaceutical inhibition in rhabdomyosarcoma, resulting in beta-catenin activation and modulation of focal adhesion kinase, with positive effects on myogenic marker expression and a reduction in proliferation and invasion. In addition, the chemical inhibitor WAY-262611 was able to impair tumor cell survival in vivo, suggesting DKK-1 as a potential molecular target for novel therapeutic strategies in RMS patients, especially those with high DKK-1 expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Y. Taank, N. Agnihotri
Summary: Aberrant Wnt/beta-catenin signaling plays a central role in the carcinogenesis of colorectal cancer. While targeting this pathway holds potential for treatment, current approaches have not been successful in clinical practice, prompting the need for new strategies. Recent studies have identified beta-catenin regulators that show promise in modulating the pathway and influencing the development of CRC and other solid tumors. Further research on the detailed mechanisms of these regulators may lead to improved treatment approaches.
CLINICAL & TRANSLATIONAL ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuangyi Cai, Thomas Hu, Mythreye Venkatesan, Mayar Allam, Frank Schneider, Suresh S. Ramalingam, Shi-Yong Sun, Ahmet F. Coskun
Summary: Protein-protein interaction networks are altered in multi-gene dysregulations in many disorders. Image-based protein multiplexing sheds light on signaling pathways to dissect cell-to-cell heterogeneity, previously masked by the bulk assays. RapMIF is a rapid multiplexed immunofluorescence method that can measure the spatial protein maps of up to 25 proteins, providing combinatorial signaling states and enabling precision therapy design.
Article
Dentistry, Oral Surgery & Medicine
Zeliha Guney, Sivge Kurgan, Canan Onder, Mahmure Ayse Tayman, Oemer Gunhan, Alpdogan Kantarci, Muhittin Abdulkadir Serdar, Meral Gunhan
Summary: This study aimed to evaluate the activity of the Wnt/beta-catenin signaling pathway in gingival samples obtained from patients with periodontitis. The results showed that periodontal inflammation disrupts the Wnt/beta-catenin signaling pathway, leading to tissue inflammation and changes in morphology. This finding may shed light on the pathogenesis of periodontal disease.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jovana Bisevac, Kirankumar Katta, Goran Petrovski, Morten Carstens Moe, Agate Noer
Summary: This study investigated the impact of Wnt/β-catenin signaling activation on human limbal epithelial stem cells (hLESCs) using the GSK-3 inhibitor LY2090314. The results showed that activation of the Wnt/β-catenin signaling pathway led to downregulation of stemness genes and upregulation of differentiation genes, as well as reduced cell proliferation and stem cell maintenance capability.
Article
Multidisciplinary Sciences
Mary Jo Cantoria, Elaheh Alizadeh, Janani Ravi, Reeba P. Varghese, Nawat Bunnag, Kelvin W. Pond, Arminja N. Kettenbach, Yashi Ahmed, Andrew L. Paek, John J. Tyson, Konstantin Doubrovinski, Ethan Lee, Curtis A. Thorne
Summary: In this study, it was found that the cellular response to Wnt ligands is proportional to their concentration. Positive feedback between the scaffold protein Axin and the kinase GSK3 resulted in bistable behavior of the destruction complex. Experimental evidence showed that the cellular concentration of beta-catenin exhibited an all-or-none response with sustained memory.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Integrative & Complementary Medicine
Jian-Qiang Wang, Yu Dong, Zi-Meng Feng, Mei-Ling Fan, Jia-Yu Yang, Jun-Nan Hu, En-Bo Cai, Hong-Yan Zhu, Wei Li, Zi Wang
Summary: This study conducted a series of experiments in vivo and in vitro to investigate the protective effects and possible mechanisms of ginsenoside Re (G-Re) against chemotherapy-induced intestinal damage. The results showed that G-Re improved oxidative damage, cytotoxicity, and apoptosis caused by cisplatin, and inhibited the activation of Wnt/β-catenin signaling. Therefore, G-Re may be a potential candidate drug for the treatment of intestinal injury.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Review
Cell Biology
Ian James Bell, Matthew Sheldon Horn, Terence John Van Raay
Summary: The non-canonical Wnt signaling pathways have different roles in cancer and development, involving calcium and Vangl2. Cells tend to choose non-canonical Wnt signaling over canonical Wnt signaling when presented with a choice, but this occurs rarely in vivo.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Abhishek Sinha, Vinson B. Fan, Aravinda-Bharathi Ramakrishnan, Nicole Engelhardt, Jennifer Kennell, Ken M. Cadigan
Summary: SOX9 promotes turnover of β-catenin independently of the destruction complex and proteasome in mammalian cell culture. This requires the activation of MAML2, which acts as a coactivator for SOX9 in antagonizing the Wnt/β-catenin pathway. This newly identified pathway provides a parallel mechanism for restricting Wnt/β-catenin signaling.
Article
Chemistry, Multidisciplinary
Daniel Hugenbusch, Marc Lehr, Jan-Simon von Glasenapp, Anna J. McConnell, Rainer Herges
Summary: We report the photo-switching properties of two regioisomeric diazocine ligands. The self-assembly of these ligands with Co-II ions was studied, and reversible transformation between different ligand configurations and Co-II complexes was achieved through light-controlled switching.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Biochemistry & Molecular Biology
Nives Pecina-Slaus, Sara Anicic, Anja Bukovac, Anja Kafka
Summary: In the search for improved antitumor therapies, inhibition of the Wnt signaling pathway has emerged as a promising target due to its altered functioning in human tumors. The pathway's frequent mutations and epigenetic changes in tumors have promoted the investigation of Wnt signaling inhibitors. Studies have shown that knocking down specific components of the pathway inhibits tumor growth. This review discusses the inhibitors of the canonical Wnt pathway, evaluates their effectiveness as therapeutics, and examines their potential side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Yang Zhou, Jiang Xu, Haichang Luo, Xiangjing Meng, Ming Chen, Di Zhu
Summary: Abnormal activation of the Wnt/beta-catenin signaling pathway is closely related to tumorigenesis and immune surveillance, leading to increased resistance to immunotherapy.
Review
Cell Biology
Teresa T. Bonello, Mark Peifer
JOURNAL OF CELL BIOLOGY
(2019)
Editorial Material
Cell Biology
Kia Z. Perez-Vale, Mark Peifer
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Andrew J. Spracklen, Emma M. Thornton-Kolbe, Alison N. Bonner, Alexandru Florea, Peter J. Compton, Rodrigo Fernandez-Gonzalez, Mark Peifer
MOLECULAR BIOLOGY OF THE CELL
(2019)
Article
Developmental Biology
Teresa T. Bonello, Wangsun Choi, Mark Peifer
Article
Biochemistry & Molecular Biology
Mario Aguilar-Aragon, Teresa T. Bonello, Graham P. Bell, Georgina C. Fletcher, Barry J. Thompson
Review
Developmental Biology
Kia Z. Perez-Vale, Mark Peifer
Article
Cell Biology
Edward M. Rogers, S. Colby Allred, Mark Peifer
Summary: Research on the long intrinsically-disordered region (IDR) of Abelson (Abl) revealed its essential role in the viability of fruit fly embryos and adults, as well as its importance in cell shape changes, embryonic morphogenesis, and the regulation of protein stability.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Cell Biology
Kia Z. Perez-Vale, Kristi D. Yow, Ruth Johnson, Amy E. Byrnes, Tara M. Finegan, Kevin C. Slep, Mark Peifer
Summary: The study reveals the critical role of Canoe protein in mediating the linkage between junctions and the actomyosin cytoskeleton, primarily through its RA domains binding to Rap1. Unexpectedly, PDZ and FAB domains are not essential for the function, with FAB domain stabilizing junctions under elevated force. The findings support a model where junctional robustness is derived from a large protein network formed through multivalent interactions.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Hui-Chia Yu-Kemp, Rachel A. Szymanski, Daniel B. Cortes, Nicole C. Gadda, Madeline L. Lillich, Amy S. Maddox, Mark Peifer
Summary: Epithelial cells assemble specialized actomyosin structures at E-Cadherin-based cell-cell junctions, and the force exerted drives cell shape change during morphogenesis. The mechanisms behind this structure formation are unclear, but research has shown that besides individual actin assembly pathways, micron-scale supramolecular myosin arrays also play a role in bundling actin at mature junctions, aiding in cell shape change.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Teresa T. Bonello, Danfeng Cai, Georgina C. Fletcher, Kyler Wiengartner, Victoria Pengilly, Kimberly S. Lange, Zhe Liu, Jennifer Lippincott-Schwartz, Jennifer M. Kavran, Barry J. Thompson
Summary: The Hippo pathway activates the Hpo kinase through the formation of biomolecular condensates, which are regulated by various factors. This mechanism is also found in human cells.
Article
Developmental Biology
Teresa Bonello, Mario Aguilar-Aragon, Alexander Tournier, Barry J. Thompson, Joseph P. Campanale
Summary: Adherens junctions play a crucial role in maintaining apical-basal polarization in epithelial cells by providing a diffusion barrier that restricts the spread of polarity determinants along the membrane for precise domain size control. Movement of adherens junctions in response to mechanical forces enables spontaneous adjustment of apical versus basolateral domain size as an emergent property of the polarising system.
CELLS & DEVELOPMENT
(2021)
Article
Developmental Biology
Teresa T. Bonello, Kia Z. Perez-Vale, Kaelyn D. Sumigray, Mark Peifer