3D variability analysis: Resolving continuous flexibility and discrete heterogeneity from single particle cryo-EM

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Cryo-electron Microscopy Structure of the Acinetobacter baumannii 70S Ribosome and Implications for New Antibiotic Development

(2020) Christopher E. Morgan et al.   mBio

A processive rotary mechanism couples substrate unfolding and proteolysis in the ClpXP degradation machinery

(2020) Zev A Ripstein et al.   eLife

Propagation of Conformational Coordinates Across Angular Space in Mapping the Continuum of States from Cryo-EM Data by Manifold Embedding

(2020) Suvrajit Maji et al.   Journal of Chemical Information and Modeling

Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction

(2020) Ali Punjani et al.   NATURE METHODS

Structure of a Signaling Cannabinoid Receptor 1-G Protein Complex

(2019) Kaavya Krishna Kumar et al.   CELL

Challenges and opportunities in cryo-EM single-particle analysis

(2019) Dmitry Lyumkis   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Basis of Nav1.7 Inhibition by a Gating-Modifier Spider Toxin

(2019) Hui Xu et al.   CELL

Cryo-EM reconstruction of continuous heterogeneity by Laplacian spectral volumes

(2019) Amit Moscovich et al.   INVERSE PROBLEMS

Structural basis for the docking of mTORC1 on the lysosomal surface

(2019) Kacper B. Rogala et al.   SCIENCE

Cryo-EM reveals an asymmetry in a novel single-ring viral chaperonin

(2019) Tatiana B. Stanishneva-Konovalova et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo–electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B

(2019) Ana S. Ramírez et al.   SCIENCE

Structure and dynamics of GPCR signaling complexes

(2018) Daniel Hilger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural Variability from Noisy Tomographic Projections

(2018) Joakim Andén et al.   SIAM Journal on Imaging Sciences

Structure and dynamics of GPCR signaling complexes

(2018) Daniel Hilger et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

(2018) Takanori Nakane et al.   eLife

cisTEM, user-friendly software for single-particle image processing

(2018) Timothy Grant et al.   eLife

emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging

(2018) Benjamin A. Himes et al.   NATURE METHODS

Building Proteins in a Day: Efficient 3D Molecular Structure Estimation with Electron Cryomicroscopy

(2017) Ali Punjani et al.   IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE

Structures of transcription pre-initiation complex with TFIIH and Mediator

(2017) S. Schilbach et al.   NATURE

Structure of a pre-catalytic spliceosome

(2017) Clemens Plaschka et al.   NATURE

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

Modular Assembly of the Bacterial Large Ribosomal Subunit

(2016) Joseph H. Davis et al.   CELL

Crystal Structure of the Human Cannabinoid Receptor CB 1

(2016) Tian Hua et al.   CELL

High resolution single particle refinement in EMAN2.1

(2016) James M. Bell et al.   METHODS

Continuous changes in structure mapped by manifold embedding of single-particle data in cryo-EM

(2016) Joachim Frank et al.   METHODS

Directly reconstructing principal components of heterogeneous particles from cryo-EM images

(2015) Hemant D. Tagare et al.   JOURNAL OF STRUCTURAL BIOLOGY

2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

(2015) Melody G Campbell et al.   eLife

Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

(2014) Wilson Wong et al.   eLife

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

A Bayesian View on Cryo-EM Structure Determination

(2011) Sjors H.W. Scheres   JOURNAL OF MOLECULAR BIOLOGY

Clustering and variance maps for cryo-electron tomography using wedge-masked differences

(2011) John M. Heumann et al.   JOURNAL OF STRUCTURAL BIOLOGY

Identifying Conformational States of Macromolecules by Eigen-Analysis of Resampled Cryo-EM Images

(2011) Pawel A. Penczek et al.   STRUCTURE