Neutralization of SARS‐CoV‐2 by highly potent, hyperthermostable, and mutation‐tolerant nanobodies

Prospective mapping of viral mutations that escape antibodies used to treat COVID-19

(2021) Tyler N. Starr et al.   SCIENCE

Structure-guided multivalent nanobodies block SARS-CoV-2 infection and suppress mutational escape

(2021) Paul-Albert Koenig et al.   SCIENCE

Mechanism of SARS-CoV-2 polymerase stalling by remdesivir

(2021) Goran Kokic et al.   Nature Communications

No higher infectivity but immune escape of SARS-CoV-2 501Y.V2 variants

(2021) Qianqian Li et al.   CELL

Extremely potent human monoclonal antibodies from COVID-19 convalescent patients

(2021) Emanuele Andreano et al.   CELL

Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced humoral immunity

(2021) Wilfredo F. Garcia-Beltran et al.   CELL

Evidence of escape of SARS-CoV-2 variant B.1.351 from natural and vaccine induced sera

(2021) Daming Zhou et al.   CELL

SARS-CoV-2 501Y.V2 escapes neutralization by South African COVID-19 donor plasma

(2021) Constantinos Kurt Wibmer et al.   NATURE MEDICINE

SARS-CoV-2 variants B.1.351 and P.1 escape from neutralizing antibodies

(2021) Markus Hoffmann et al.   CELL

SARS-CoV-2 evolution in an immunocompromised host reveals shared neutralization escape mechanisms

(2021) Sarah A. Clark et al.   CELL

Nanobody cocktails potently neutralize SARS-CoV-2 D614G N501Y variant and protect mice

(2021) Phillip Pymm et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nanobodies from camelid mice and llamas neutralize SARS-CoV-2 variants

(2021) Jianliang Xu et al.   NATURE

The folate antagonist methotrexate diminishes replication of the coronavirus SARS-CoV-2 and enhances the antiviral efficacy of remdesivir in cell culture models

(2021) Kim M. Stegmann et al.   VIRUS RESEARCH

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

(2020) Victor M Corman et al.   Eurosurveillance

A pneumonia outbreak associated with a new coronavirus of probable bat origin

(2020) Peng Zhou et al.   NATURE

Structural basis for the recognition of the SARS-CoV-2 by full-length human ACE2

(2020) Renhong Yan et al.   SCIENCE

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Structural and Functional Basis of SARS-CoV-2 Entry by Using Human ACE2

(2020) Qihui Wang et al.   CELL

Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies

(2020) Daniel Wrapp et al.   CELL

Structure of the SARS-CoV-2 spike receptor-binding domain bound to the ACE2 receptor

(2020) Jun Lan et al.   NATURE

Remdesivir for the Treatment of Covid-19 — Preliminary Report

(2020) John H. Beigel et al.   NEW ENGLAND JOURNAL OF MEDICINE

Neutralizing nanobodies bind SARS-CoV-2 spike RBD and block interaction with ACE2

(2020) Jiangdong Huo et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2

(2020) Kui K. Chan et al.   SCIENCE

Structure-based design of prefusion-stabilized SARS-CoV-2 spikes

(2020) Ching-Lin Hsieh et al.   SCIENCE

Effect of Remdesivir vs Standard Care on Clinical Status at 11 Days in Patients With Moderate COVID-19

(2020) Christoph D. Spinner et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Exploring cellular biochemistry with nanobodies

(2020) Ross W Cheloha et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

COVID-19 antibodies on trial

(2020) Laura DeFrancesco   NATURE BIOTECHNOLOGY

Convalescent plasma treatment of severe COVID-19: a propensity score–matched control study

(2020) Sean T. H. Liu et al.   NATURE MEDICINE

An alpaca nanobody neutralizes SARS-CoV-2 by blocking receptor interaction

(2020) Leo Hanke et al.   Nature Communications

Development of humanized tri-specific nanobodies with potent neutralization for SARS-CoV-2

(2020) Jianbo Dong et al.   Scientific Reports

Cryo-EM Structures of SARS-CoV-2 Spike without and with ACE2 Reveal a pH-Dependent Switch to Mediate Endosomal Positioning of Receptor-Binding Domains

(2020) Tongqing Zhou et al.   Cell Host & Microbe

SARS-CoV-2 Neutralizing Antibody LY-CoV555 in Outpatients with Covid-19

(2020) Peter Chen et al.   NEW ENGLAND JOURNAL OF MEDICINE

High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme

(2020) Thomas J. Esparza et al.   Scientific Reports

Picomolar SARS-CoV-2 Neutralization Using Multi-Arm PEG Nanobody Constructs

(2020) Ainhoa Moliner-Morro et al.   Biomolecules

Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix

(2019) Dorothee Liebschner et al.   Acta Crystallographica Section D-Structural Biology

Real-time cryo-electron microscopy data preprocessing with Warp

(2019) Dimitry Tegunov et al.   NATURE METHODS

Exploiting Nanobodies’ Singular Traits

(2018) Jessica R. Ingram et al.   Annual Review of Immunology

New tools for automated high-resolution cryo-EM structure determination in RELION-3

(2018) Jasenko Zivanov et al.   eLife

cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination

(2017) Ali Punjani et al.   NATURE METHODS

MolProbity: More and better reference data for improved all-atom structure validation

(2017) Christopher J. Williams et al.   PROTEIN SCIENCE

Intramolecular trimerization, a novel strategy for making multispecific antibodies with controlled orientation of the antigen binding domains

(2016) Ana Alvarez-Cienfuegos et al.   Scientific Reports

Developability Assessment During the Selection of Novel Therapeutic Antibodies

(2015) Alexander Jarasch et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation

(2015) Tino Pleiner et al.   eLife

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Dendroscope 3: An Interactive Tool for Rooted Phylogenetic Trees and Networks

(2012) Daniel H. Huson et al.   SYSTEMATIC BIOLOGY

Data processing and analysis with theautoPROCtoolbox

(2011) Clemens Vonrhein et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega

(2011) F. Sievers et al.   Molecular Systems Biology

Engineered Single-Domain Antibodies with High Protease Resistance and Thermal Stability

(2011) Greg Hussack et al.   PLoS One

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability

(2010) Deborah S. Goldberg et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Crystal structure of the human collagen XV trimerization domain: A potent trimerizing unit common to multiplexin collagens

(2010) Jacqueline A. Wirz et al.   MATRIX BIOLOGY

A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation

(2010) Dorothy Beckett et al.   PROTEIN SCIENCE

Crystal Structure of Human Collagen XVIII Trimerization Domain: A Novel Collagen Trimerization Fold

(2009) Sergei P. Boudko et al.   JOURNAL OF MOLECULAR BIOLOGY

In Vivo Tumor Targeting and Imaging with Engineered Trivalent Antibody Fragments Containing Collagen-Derived Sequences

(2009) Ángel M. Cuesta et al.   PLoS One

Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet

(2008) Yasmina Abdiche et al.   ANALYTICAL BIOCHEMISTRY