Article
Materials Science, Characterization & Testing
Guiya Deng, Xianchao Jiang, Zihao Song, Chengming Li, Yuepeng Guan, Shangwei Guo, Yaqin Huang
Summary: Collagen from chicken skin is used in food to improve health benefits, but current methods for identifying chicken-derived components lack accuracy. A sensitive method based on LC-MS was developed using two marker peptides to detect chicken-derived components in collagen-containing foods. The method has a very low detection limit and can authenticate chicken skin-derived components in processed meat.
Article
Engineering, Biomedical
Stylianos O. Sarrigiannidis, Oana Dobre, Alexandre Rodrigo Navarro, Matthew J. Dalby, Cristina Gonzalez-Garcia, Manuel Salmeron-Sanchez
Summary: Researchers have developed a recombinant dual affinity protein fragment that can immobilize bone morphogenic protein 2 (BMP-2) in collagen sponges, addressing the low affinity issue between BMP-2 and collagen. This technology enables osteogenesis with ultra-low doses of BMP-2 and enhances the biological activity of collagen, providing a potential pathway for clinical translation without complex chemistries or manufacturing changes.
MATERIALS TODAY BIO
(2023)
Article
Immunology
Lukas Pekar, Daniel Klewinghaus, Paul Arras, Stefania C. Carrara, Julia Harwardt, Simon Krah, Desislava Yanakieva, Lars Toleikis, Vaughn V. Smider, Harald Kolmar, Stefan Zielonka
Summary: In this study, EGFR-specific ultralong CDR-H3 antibodies were generated from cattle immunization and yeast surface display, demonstrating potent antibody-dependent cell-mediated cytotoxicity against EGFR-overexpressing tumor cells. The antibodies showed epitope targeting of specific domains of EGFR and retained main properties of parental antibodies when engineered as Knobbodies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Chemical
M. J. B. Matos, F. Trovao, J. Goncalves, U. Rothbauer, M. G. Freire, A. M. J. B. Barbosa, A. S. Pina, A. C. A. Roque
Summary: Antibody technologies are a successful case in the biopharmaceutical industry, with researchers developing affinity adsorbents inspired by natural and engineered antibody-binding ligands to capture full-length antibodies and fragments in a single-step recovery process.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Aline Bauer, Sven Klassa, Anja Herbst, Cristina Maccioni, William Abhamon, Noria Segueni, Yulia Kaluzhny, Morgan Campbell Hunter, Cornelia Halin
Summary: Activated leukocyte cell adhesion molecule (ALCAM) plays a role in various immune-mediated disorders. Researchers have developed stability- and affinity-improved anti-ALCAM antibody fragments that can block ALCAM interactions. These fragments have potential for topical application in diseases such as asthma.
Article
Biology
Maria Geis, Boris Nowotny, Marc-Dominic Bohn, Dina Kouhestani, Hermann Einsele, Thomas Bumm, Gernot Stuhler
Summary: Geis et al. investigate the potential application of hemibodies, a pair of complementary antibody fragments that redirect T cells against cancer-defining antigen combinations, such as CD38 and SLAMF7, to target multiple myeloma. Their study provides evidence that hemibodies can be developed for effective immunotherapy against multiple myeloma.
COMMUNICATIONS BIOLOGY
(2021)
Article
Engineering, Biomedical
Hidenori Tani, Eiji Kobayashi, Shinomi Yagi, Keisuke Tanaka, Kotaro Kameda-Haga, Shinsuke Shibata, Nobuko Moritoki, Kaworu Takatsuna, Taijun Moriwaki, Otoya Sekine, Tomohiko C. Umei, Yuika Morita, Yusuke Soma, Yoshikazu Kishino, Hideaki Kanazawa, Jun Fujita, Shunji Hattori, Shugo Tohyama
Summary: This study found that heart-derived collagen is more effective in heart tissue engineering and can lead to more mature engineered heart tissue.
Article
Ecology
Mengdi Yang, Pu-Ting Dong, Lujia Cen, Wenyuan Shi, Xuesong He, Jiahe Li
Summary: Host mucosal barriers possess defense molecules including antimicrobial peptides and immunoglobulins to maintain host-microbe homeostasis. We have recently discovered small RNAs (sRNAs) that mediate interactions between human oral keratinocytes and Fusobacterium nucleatum, a pathobiont implicated in extra-oral diseases. These sRNAs, which are tRNA-derived (tsRNAs), exhibit gene regulatory functions and can inhibit the growth of F. nucleatum without any delivery vehicle in nanomolar concentrations.
Article
Microbiology
Linda De Vooght, Karin De Ridder, Shahid A. Hussain, Benoit Stijlemans, Patrick A. De Baetselier, Guy Caljon, Jan A. van den Abbeele
Summary: This experimental study successfully genetically modified the gut symbiont of tsetse flies to interfere with the development of trypanosomes, providing a promising strategy for controlling vector-borne diseases. The study also highlights the potential use of this symbiont-based delivery system as a laboratory tool for studying the molecular determinants of vector-pathogen associations.
Article
Engineering, Biomedical
Kaveh Roshanbinfar, Maria Kolesnik-Gray, Miriam Angeloni, Stefan Schruefer, Maren Fiedler, Dirk W. Schubert, Fulvia Ferrazzi, Vojislav Krstic, Felix B. Engel
Summary: Cardiac tissue engineering is a promising strategy to prevent heart failure, but several issues remain unsolved. This study develops a biohybrid hydrogel that enhances the beating properties of engineered cardiac tissues and allows for concurrent drug release.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Immunology
Bruce D. Wines, Liriye Kurtovic, Halina M. Trist, Sandra Esparon, Ester Lopez, Klasina Chappin, Li-Jin Chan, Francesca L. Mordant, Wen Shi Lee, Nicholas A. Gherardin, Sheila K. Patel, Gemma E. Hartley, Phillip Pymm, James P. Cooney, James G. Beeson, Dale Godfrey, Louise M. Burrell, Menno C. van Zelm, Adam K. Wheatley, Amy W. Chung, Wai-Hong Tham, Kanta Subbarao, Stephen J. Kent, P. Mark Hogarth
Summary: Joining a function-enhanced Fc-portion of human IgG to the SARS-CoV-2 entry receptor ACE2 produces an antiviral decoy with strain transcending virus neutralizing activity. Fc-modification was utilized to optimize SARS-CoV-2 neutralization and Fc-effector functions of ACE2-Fc decoy proteins. Different Fc-modifications had distinct effects on neutralization and effector functions.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Johanna Simon, Michael Fichter, Gabor Kuhn, Maximilian Brueckner, Cinja Kappel, Jenny Schunke, Tanja Klaus, Stephan Grabbe, Katharina Landfester, Volker Mailaender
Summary: The major challenge in nanocarrier-based anti-cancer vaccination approaches is the targeted delivery of antigens and immunostimulatory agents to specific cells, such as dendritic cells, to induce strong anti-tumor responses. This study presents a surface functionalization strategy to attach antibodies onto nanocarriers, enabling specific targeting and avoiding non-specific uptake. The development of antibody-functionalized nanocarriers for dendritic cell-based vaccination in cancer immunotherapy is greatly facilitated by this novel conjugation technique.
Article
Chemistry, Multidisciplinary
Eva Avilla-Royo, Ladina Vonzun, Frauke Seehusen, Queralt Vallmajo-Martin, Flurina Famos, Lukas Moser, Katharina Gegenschatz-Schmid, Lisa Amanda Krattiger, Nele Struebing, Miriam Weisskopf, Ueli Moehrlen, Nicole Ochsenbein-Koelble, Martin Ehrbar
Summary: This study introduces TG-PEG hydrogels loaded with PDGF-BB for promoting fetal membrane healing. In vitro experiments showed that these hydrogels can stimulate the migration and proliferation of amniotic cells. In vivo, the hydrogels effectively seal fetal membrane and uterus defects and recruit host cells for early healing.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Medicinal
Ioanna Lapi, Ourania Kolliniati, Tone Aspevik, Eleftherios E. Deiktakis, Konstantinos Axarlis, Maria G. Daskalaki, Eirini Dermitzaki, Maria Tzardi, Sotirios C. Kampranis, Zouhir El Marsni, Katerina C. Kousoulaki, Christos Tsatsanis, Maria Venihaki
Summary: Dietary supplementation with marine collagen-enriched supplements promoted tissue repair in a model of cutaneous wound healing, accelerating the healing process. The collagen-containing supplements increased the expression of chemokines for immune cell recruitment and elevated expression of the angiogenic factor VEGF-A, leading to enhanced collagen deposition in the damaged tissues.
Article
Biotechnology & Applied Microbiology
Wenzhi Tan, Mai Thi-Quynh Duong, Chaohui Zuo, Yeshan Qin, Ying Zhang, Yanxia Guo, Yeongjin Hong, Jin Hai Zheng, Jung-Joon Min
Summary: Pancreatic cancer is resistant to conventional therapies due to abundant stromal cells and poor immune cell infiltration. This study used engineered Salmonella typhimurium expressing ClyA to target cancer stromal cells and cancer cells, effectively treating pancreatic cancer in mice. The bacteria accumulated in tumor tissues and secreted ClyA into the tumor microenvironment, leading to inhibition of cancer growth and increased immune cell infiltration. This approach provides a novel strategy for treating pancreatic cancer by combining anticancer and antistromal therapy.
Review
Medicine, Research & Experimental
Antonio Tapia-Galisteo, Marta Compte, Luis alvarez-Vallina, Laura Sanz
Summary: Multispecific antibodies have the potential to overcome current limitations and have important implications in cancer therapy.
Article
Biochemistry & Molecular Biology
Ainara Cano, Mercedes Vazquez-Chantada, Javier Conde-Vancells, Aintzane Gonzalez-Lahera, David Mosen-Ansorena, Francisco J. J. Blanco, Karine Clement, Judith Aron-Wisnewsky, Albert Tran, Philippe Gual, Carmelo Garcia-Monzon, Joan Caballeria, Azucena Castro, Maria Luz Martinez-Chantar, Jose M. Mato, Huiping Zhu, Richard H. H. Finnell, Ana M. M. Aransay
Summary: Low serum folate levels are associated with metabolic associated fatty liver disease (MAFLD). The role of the folate transporter gene (SLC19A1) in lipid accumulation during the onset of MAFLD was assessed through genomic, transcriptomic, and metabolomic techniques. SNPs rs1051266 and rs3788200 were significantly associated with fatty liver development, and the lack of functional SLC19A1 affected gene regulation and lipid metabolism, leading to lipid droplet accumulation in hepatocytes.
Article
Medicine, Research & Experimental
Ana Antunes, Luis Alvarez-Vallina, Federico Bertoglio, Nicolas Bouquin, Stephanie Cornen, Francis Duffieux, Pierre Ferre, Raphaelle Gillet, Christian Jorgensen, Mark B. Leick, Bernard Maillere, Helene Negre, Mireia Pelegrin, Nicolas Poirier, Dietmar Reusch, Bruno Robert, Guy Serre, Alain Vicari, Martin Villalba, Christoph Volpers, Gavin Vuddamalay, Herve Watier, Thierry Wurch, Lennart Zabeau, Stefan Zielonka, Baolin Zhang, Alain Beck, Pierre Martineau
Summary: The annual Antibody Industrial Symposium brought together scientists from academia and industry to discuss new advancements in antibody engineering and cell-based therapies. The symposium also addressed production and intellectual property protection issues.
Article
Multidisciplinary Sciences
Jingyi Zhao, Vincent DiGiacomo, Mariola Ferreras-Gutierrez, Shiva Dastjerdi, Alain Ibanez de Opakua, Jong-Chan Park, Alex Luebbers, Qingyan Chen, Aaron Beeler, Francisco J. Blanco, Mikel Garcia-Marcos
Summary: IGGi-11 selectively inhibits noncanonical activation of heterotrimeric G-protein signaling, blocking tumor cell signaling and inhibiting metastatic cancer cell invasion without interfering with canonical GPCR signaling mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Oncology
Laura Rubio-Perez, Rodrigo Lazaro-Gorines, Seandean L. Harwood, Marta Compte, Rocio Navarro, Antonio Tapia-Galisteo, Jaume Bonet, Belen Blanco, Simon Lykkemark, Angel Ramirez-Fernandez, Mariola Ferreras-Gutierrez, Carmen Dominguez-Alonso, Laura Diez-Alonso, Alejandro Segura-Tudela, Oana Hangiu, Ainhoa Erce-Llamazares, Francisco J. Blanco, Cruz Santos, Jose L. Rodriguez-Peralto, Laura Sanz, Luis Alvarez-Vallina
Summary: In this study, a PD-L1/EGFR symmetric bispecific antibody was developed, which could simultaneously inhibit EGFR-mediated proliferation, effectively block PD-1/PD-L1 interaction, and induce strong antigen-specific antibody-dependent cellular cytotoxicity activity in vitro. Potent therapeutic efficacies of this antibody were observed in two different humanized mouse models.
Review
Oncology
Antonio Tapia-Galisteo, Luis alvarez-Vallina, Laura Sanz
Summary: Immune cell engagers are modified antibodies that have one arm binding a tumor-associated antigen and another arm binding an activating receptor in immune effector cells. These engagers have the potential to revolutionize the treatment of hematological malignancies and are more potent than conventional monoclonal antibodies. The field is growing rapidly, with multiple formats and targets currently in clinical trials, and trispecific antibodies show even more promise by targeting additional tumor-associated antigens or costimulatory receptors.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Rodrigo Lazaro-Gorines, Patricia Perez, Ignacio Heras-Murillo, Irene Adan-Barrientos, Guillermo Albericio, David Astorgano, Sara Flores, Joanna Luczkowiak, Nuria Labiod, Seandean L. Harwood, Alejandro Segura-Tudela, Laura Rubio-Perez, Yudhi Nugraha, Xiaoran Shang, Yuxing Li, Carlos Alfonso, Kaylin A. Adipietro, Dinendra L. Abeyawardhane, Rocio Navarro, Marta Compte, Wenbo Yu, Alexander D. Mackerell, Laura Sanz, David J. Weber, Francisco J. Blanco, Mariano Esteban, Edwin Pozharski, Raquel Godoy-Ruiz, Ines G. Munoz, Rafael Delgado, David Sancho, Juan Garcia-Arriaza, Luis Alvarez-Vallina
Summary: In this study, a broadly neutralizing antibody (nAb) TNT was generated, which showed high-avidity neutralizing interaction by simultaneously binding to all six RBD epitopes. By fusing an anti-DNGR-1 scFv to TNT, a bispecific trimerbody TN(T)DNGR-1 was generated, which targeted neutralized virions to cDC1s and promoted T cell cross-priming. Therapeutic administration of TN(T)DNGR-1 protected mice from lethal SARS-CoV-2 infection and enhanced virus-specific humoral and CD8(+) T cell responses.
Article
Biochemistry & Molecular Biology
Javier Narbona, Luisa Hernandez-Baraza, Ruben G. Gordo, Laura Sanz, Javier Lacadena
Summary: Immunotoxins are chimeric molecules that combine the specificity of an antibody-based targeting domain and the cytotoxic potency of a toxin. This study designed and characterized several immunotoxin constructs using nanobodies as targeting domains against EGFR. The results confirm the therapeutic potential of a-sarcin-based nanoITXs.
Article
Clinical Neurology
Jorge Cabrera-Montes, Daniel T. Aguirre, Jesus Vinas-Lopez, Laura Lorente-Herraiz, Lucia Recio-Poveda, Virginia Albinana, Julian Perez-Perez, Luisa M. Botella, Angel M. Cuesta
Summary: This case report illustrates the importance of deeper genetic analyses in inherited rare diseases, by presenting the case of a VHL patient with severe manifestations. The analysis revealed pathogenic mutations in CHEK2 and the loss of a VHL allele, as well as additional mutations in BRAF1 and PTPN11 in the tumor sample. The study concludes that the severity of VHL in this patient is attributed to both VHL and CHEK2 mutations.
ACTA NEUROCHIRURGICA
(2023)
