SELEX: Critical factors and optimization strategies for successful aptamer selection

Aptamers against live targets: Is in vivo SELEX finally coming to edge?

(2020) Mayte Sola et al.   Molecular Therapy-Nucleic Acids

Novel Strategies to Optimize the Amplification of Single-Stranded DNA

(2020) Atef Nehdi et al.   Frontiers in Bioengineering and Biotechnology

Sensing with Nanopores and Aptamers: A Way Forward

(2020) Lucile Reynaud et al.   SENSORS

A Detailed Protein-SELEX Protocol Allowing Visual Assessments of Individual Steps for a High Success Rate

(2019) Tao Wang et al.   Human Gene Therapy Methods

Aptamers as quality control tool for production, storage and biosimilarity of the anti-CD20 biopharmaceutical rituximab

(2019) Sabrina Wildner et al.   Scientific Reports

Next-level riboswitch development—implementation of Capture-SELEX facilitates identification of a new synthetic riboswitch

(2019) Adrien Boussebayle et al.   NUCLEIC ACIDS RESEARCH

Click-Particle Display for Base-Modified Aptamer Discovery

(2019) Chelsea K.L. Gordon et al.   ACS Chemical Biology

Quantitative characterization of oligomeric state of G-quadruplex antithrombin aptamers by size exclusion HPLC

(2019) Rugiya R. Alieva et al.   MENDELEEV COMMUNICATIONS

Structure and Function of Multimeric G-Quadruplexes

(2019) Sofia Kolesnikova et al.   MOLECULES

Inside the Black Box: What Makes SELEX Better?

(2019) Natalia Komarova et al.   MOLECULES

Aptamer chemistry

(2018) Pascal Röthlisberger et al.   ADVANCED DRUG DELIVERY REVIEWS

Hairpin-Contained i-Motif Based Fluorescent Ratiometric Probe for High-Resolution and Sensitive Response of Small pH Variations

(2018) Wenjie Ma et al.   ANALYTICAL CHEMISTRY

Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics

(2018) James B. McGivney et al.   ANALYTICAL CHEMISTRY

Thermodynamic study of aptamers binding to their target proteins

(2018) Taiichi Sakamoto et al.   BIOCHIMIE

Selection of aptamers for AMACR detection from DNA libraries with different primers

(2018) Deng-Kai Yang et al.   RSC Advances

SELEX methods on the road to protein targeting with nucleic acid aptamers

(2018) Payam Bayat et al.   BIOCHIMIE

Ultra-pH-responsive split i-motif based aptamer anchoring strategy for specific activatable imaging of acidic tumor microenvironment

(2018) Yanli Lei et al.   CHEMICAL COMMUNICATIONS

Magnetic Separation-Based Multiple SELEX for Effectively Selecting Aptamers against Saxitoxin, Domoic Acid, and Tetrodotoxin

(2018) Huajie Gu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Three decades of nucleic acid aptamer technologies: Lessons learned, progress and opportunities on aptamer development

(2018) Tao Wang et al.   BIOTECHNOLOGY ADVANCES

Post-SELEX optimization of aptamers

(2016) Shunxiang Gao et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Kinetic and Thermodynamic Analyses of Interaction between a High-Affinity RNA Aptamer and Its Target Protein

(2016) Ryo Amano et al.   BIOCHEMISTRY

Gonyautoxin 1/4 aptamers with high-affinity and high-specificity: From efficient selection to aptasensor application

(2016) Shunxiang Gao et al.   BIOSENSORS & BIOELECTRONICS

Embracing proteins: structural themes in aptamer–protein complexes

(2016) Amy D Gelinas et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

3D Cell-SELEX: Development of RNA aptamers as molecular probes for PC-3 tumor cell line

(2016) Aline G. Souza et al.   EXPERIMENTAL CELL RESEARCH

Hemin/G-quadruplex structure and activity alteration induced by magnesium cations

(2016) J. Kosman et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Improving the odds: Influence of starting pools on in vitro selection outcomes

(2016) Kelsey Pobanz et al.   METHODS

744. Optimizing Conditions for Aptamer Folding Using a High-Throughput Aptamer Fluorescence Binding and Internalization (AFBI) Assay

(2016) David D. Dickey et al.   MOLECULAR THERAPY

Methods for Improving Aptamer Binding Affinity

(2016) Hijiri Hasegawa et al.   MOLECULES

Three decades of nanopore sequencing

(2016) David Deamer et al.   NATURE BIOTECHNOLOGY

Structural basis for specific inhibition of Autotaxin by a DNA aptamer

(2016) Kazuki Kato et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Recent advances in universal TA cloning methods for use in function studies

(2016) Shuo Yao et al.   PROTEIN ENGINEERING DESIGN & SELECTION

In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies

(2016) Farhana Lipi et al.   RNA Biology

Structural basis for specific inhibition of Autotaxin by a DNA aptamer

(2016) Kazuki Kato et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Small-Molecule Binding Aptamers: Selection Strategies, Characterization, and Applications

(2016) Annamaria Ruscito et al.   Frontiers in Chemistry

Comparison of the methods for generating single-stranded DNA in SELEX

(2015) Chao Liang et al.   ANALYST

Universal CG cloning of polymerase chain reaction products

(2015) Julian Stevenson et al.   ANALYTICAL BIOCHEMISTRY

Indirect purification method provides high yield and quality ssDNA sublibrary for potential aptamer selection

(2015) Yinze Zhang et al.   ANALYTICAL BIOCHEMISTRY

A combinatorial systematic evolution of ligands by exponential enrichment method for selection of aptamer against protein targets

(2015) Bhairab Mondal et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Current approaches in SELEX: An update to aptamer selection technology

(2015) Mariia Darmostuk et al.   BIOTECHNOLOGY ADVANCES

Hi-Fi SELEX: A high-fidelity digital-PCR based therapeutic aptamer discovery platform

(2015) Eric Ouellet et al.   BIOTECHNOLOGY AND BIOENGINEERING

Analysis of In Vitro Aptamer Selection Parameters

(2015) Maureen McKeague et al.   JOURNAL OF MOLECULAR EVOLUTION

Evolution of Functional Six-Nucleotide DNA

(2015) Liqin Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Highlight of Recent Advances in Aptamer Technology and Its Application

(2015) Hongguang Sun et al.   MOLECULES

Large scale analysis of the mutational landscape in HT-SELEX improves aptamer discovery

(2015) Jan Hoinka et al.   NUCLEIC ACIDS RESEARCH

Single-Round Patterned DNA Library Microarray Aptamer Lead Identification

(2015) Jennifer A. Martin et al.   Journal of Analytical Methods in Chemistry

How Much DNA Is Lost? Measuring DNA Loss of Short-Tandem-Repeat Length Fragments Targeted by the PowerPlex 16® System Using the Qiagen MinElute Purification Kit

(2015) Brian M. Kemp et al.   HUMAN BIOLOGY

Absolute Quantification of Cell-Bound DNA Aptamers During SELEX

(2015) Meltem Avci-Adali et al.   Nucleic Acid Therapeutics

High-throughput binding characterization of RNA aptamer selections using a microplate-based multiplex microcolumn device

(2014) Kylan Szeto et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Quantitative Detection of Potassium Ions and Adenosine Triphosphate via a Nanochannel-Based Electrochemical Platform Coupled with G-Quadruplex Aptamers

(2014) Jiachao Yu et al.   ANALYTICAL CHEMISTRY

Particle Display: A Quantitative Screening Method for Generating High-Affinity Aptamers

(2014) Jinpeng Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Aptamers and SELEX in Chemistry & Biology

(2014) Michael Famulok et al.   CHEMISTRY & BIOLOGY

Library preparation methods for next-generation sequencing: Tone down the bias

(2014) Erwin L. van Dijk et al.   EXPERIMENTAL CELL RESEARCH

Optimization of SELEX: Comparison of different methods for monitoring the progress of in vitro selection of aptamers

(2014) Nina Mencin et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

By-Product Formation in Repetitive PCR Amplification of DNA Libraries during SELEX

(2014) Fabian Tolle et al.   PLoS One

Detection and Characterization of Cancer Cells and Pathogenic Bacteria Using Aptamer-Based Nano-Conjugates

(2014) Vinayakumar Gedi et al.   SENSORS

Suppression of APOBEC3-mediated restriction of HIV-1 by Vif

(2014) Yuqing Feng et al.   Frontiers in Microbiology

New Technologies Provide Quantum Changes in the Scale, Speed, and Success of SELEX Methods and Aptamer Characterization

(2014) Abdullah Ozer et al.   Molecular Therapy-Nucleic Acids

Probing high-affinity 11-mer DNA aptamer against Lup an 1 (β-conglutin)

(2013) P. Nadal et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Aptamer biosensor for label-free impedance spectroscopy detection of potassium ion based on DNA G-quadruplex conformation

(2013) Zhengbo Chen et al.   BIOSENSORS & BIOELECTRONICS

In silico maturation of binding-specificity of DNA aptamers againstProteus mirabilis

(2013) Nasa Savory et al.   BIOTECHNOLOGY AND BIOENGINEERING

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

(2013) Michiko Kimoto et al.   NATURE BIOTECHNOLOGY

Visualizing the quadruplex

(2013) Adam Siddiqui-Jain et al.   Nature Chemistry

Sol-Gel SELEX Circumventing Chemical Conjugation of Low Molecular Weight Metabolites Discovers Aptamers Selective to Xanthine

(2013) Hyunjung Bae et al.   Nucleic Acid Therapeutics

Comparison of different methods for generation of single-stranded DNA for SELEX processes

(2012) M. Svobodová et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Aptamer selection by high-throughput sequencing and informatic analysis

(2012) Shawn Hoon et al.   BIOTECHNIQUES

Theoretical consideration of selective enrichment in in vitro selection: Optimal concentration of target molecules

(2012) Takuyo Aita et al.   MATHEMATICAL BIOSCIENCES

Darwinian evolution of an alternative genetic system provides support for TNA as an RNA progenitor

(2012) Hanyang Yu et al.   Nature Chemistry

Selection is more intelligent than design: improving the affinity of a bivalent ligand through directed evolution

(2012) Kareem M. Ahmad et al.   NUCLEIC ACIDS RESEARCH

Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics

(2012) Regina Stoltenburg et al.   Journal of Analytical Methods in Chemistry

Aptamers can Discriminate Alkaline Proteins with High Specificity

(2011) Hanyang Yu et al.   CHEMBIOCHEM

Immobilization-free screening of aptamers assisted by graphene oxide

(2011) Jee-Woong Park et al.   CHEMICAL COMMUNICATIONS

Studies of the binding mechanism between aptamers and thrombin by circular dichroism, surface plasmon resonance and isothermal titration calorimetry

(2011) Po-Hsun Lin et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Comparison of Solution and Crystal Structures of PreQ1Riboswitch Reveals Calcium-Induced Changes in Conformation and Dynamics

(2011) Qi Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

(2011) Keke Shao et al.   PLoS One

Probing the SELEX Process with Next-Generation Sequencing

(2011) Tatjana Schütze et al.   PLoS One

Enhanced Functional Potential of Nucleic Acid Aptamer Libraries Patterned to Increase Secondary Structure

(2010) Karen M. Ruff et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Screening and Improvement of an Anti-VEGF DNA Aptamer

(2010) Yoshihiko Nonaka et al.   MOLECULES

Development of DNA aptamers using Cell-SELEX

(2010) Kwame Sefah et al.   Nature Protocols

Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures

(2010) Günter Mayer et al.   Nature Protocols

Conformational plasticity of RNA for target recognition as revealed by the 2.15 Å crystal structure of a human IgG–aptamer complex

(2010) Yusuke Nomura et al.   NUCLEIC ACIDS RESEARCH

In vitro selection of structure-switching, self-reporting aptamers

(2010) S. S. Oh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Computational approaches toward the design of pools for the in vitro selection of complex aptamers

(2010) X. Luo et al.   RNA

Aptamers Generated from Cell-SELEX for Molecular Medicine: A Chemical Biology Approach

(2009) Xiaohong Fang et al.   ACCOUNTS OF CHEMICAL RESEARCH

Generation of Highly Specific Aptamers via Micromagnetic Selection

(2009) Jiangrong Qian et al.   ANALYTICAL CHEMISTRY

Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains

(2009) Markus Wieland et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Effective isolation of RNA aptamer through suppression of PCR bias

(2009) Shoutaro Tsuji et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Nucleic acid contamination of glycogen used in nucleic acid precipitation and assessment of linear polyacrylamide as an alternative co-precipitant

(2009) Andrea K. Bartram et al.   BIOTECHNIQUES

Selection and elution of aptamers using nanoporous sol-gel arrays with integrated microheaters

(2009) Seung-min Park et al.   LAB ON A CHIP

Locked nucleic acid nucleoside triphosphates and polymerases: on the way towards evolution of LNA aptamers

(2009) Rakesh N. Veedu et al.   Molecular BioSystems

Upgrading SELEX Technology by Using Lambda Exonuclease Digestion for Single-Stranded DNA Generation

(2009) Meltem Avci-Adali et al.   MOLECULES

Combining use of a panel of ssDNA aptamers in the detection of Staphylococcus aureus

(2009) Xiaoxiao Cao et al.   NUCLEIC ACIDS RESEARCH

Aptamers selected against the unglycosylated EGFRvIII ectodomain and delivered intracellularly reduce membrane-bound EGFRvIII and induce apoptosis

(2008) Yingmiao Liu et al.   BIOLOGICAL CHEMISTRY

Development of DNA aptamers for cytochemical detection of acetylcholine

(2008) John G. Bruno et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL

Determination of Ochratoxin A with a DNA Aptamer

(2008) Jorge A. Cruz-Aguado et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Bioinformatic Analysis of the Contribution of Primer Sequences to Aptamer Structures

(2008) Matthew C. Cowperthwaite et al.   JOURNAL OF MOLECULAR EVOLUTION

Anticoagulant characteristics of HD1-22, a bivalent aptamer that specifically inhibits thrombin and prothrombinase

(2008) J. MÜLLER et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Single-molecule detection of folding and unfolding of the G-quadruplex aptamer in a nanopore nanocavity

(2008) Ji Wook Shim et al.   NUCLEIC ACIDS RESEARCH