Methods for Improving Aptamer Binding Affinity

Systematic selection of modified aptamer pairs for diagnostic sandwich assays

(2016) Urs A. Ochsner et al.   BIOTECHNIQUES

Improvement of the VEGF binding ability of DNA aptamers through in silico maturation and multimerization strategy

(2015) Takahiro Fukaya et al.   JOURNAL OF BIOTECHNOLOGY

A Systematic Study of the Features Critical for Designing a High Avidity Multivalent Aptamer

(2015) Xiaoching Zhao et al.   Nucleic Acid Therapeutics

Array-based Discovery of Aptamer Pairs

(2014) Minseon Cho et al.   ANALYTICAL CHEMISTRY

Structural basis of the non-coding RNA RsmZ acting as a protein sponge

(2014) Olivier Duss et al.   NATURE

Comprehensive analysis of RNA-protein interactions by high-throughput sequencing–RNA affinity profiling

(2014) Jacob M Tome et al.   NATURE METHODS

An ultra-sensitive detection of a whole virus using dual aptamers developed by immobilization-free screening

(2013) Jee-Woong Park et al.   BIOSENSORS & BIOELECTRONICS

Simultaneous improvement of specificity and affinity of aptamers againstStreptococcus mutansby in silico maturation for biosensor development

(2013) Nasa Savory et al.   BIOTECHNOLOGY AND BIOENGINEERING

In silico maturation of binding-specificity of DNA aptamers againstProteus mirabilis

(2013) Nasa Savory et al.   BIOTECHNOLOGY AND BIOENGINEERING

Discovery of Widespread GTP-Binding Motifs in Genomic DNA and RNA

(2013) Edward A. Curtis et al.   CHEMISTRY & BIOLOGY

Generation of high-affinity DNA aptamers using an expanded genetic alphabet

(2013) Michiko Kimoto et al.   NATURE BIOTECHNOLOGY

Functional-Group Specific Aptamers Indirectly Recognizing Compounds with Alkyl Amino Group

(2012) Hongcheng Mei et al.   ANALYTICAL CHEMISTRY

Selection Strategy to Generate Aptamer Pairs that Bind to Distinct Sites on Protein Targets

(2012) Qiang Gong et al.   ANALYTICAL CHEMISTRY

Multivalency as a Chemical Organization and Action Principle

(2012) Carlo Fasting et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

G-quadruplex DNA aptamers for zeatin recognizing

(2012) Cui Qi et al.   BIOSENSORS & BIOELECTRONICS

Electrochemical Detection of Vascular Endothelial Growth Factor by an Aptamer-Based Bound/Free Separation System

(2012) Koichi ABE et al.   ELECTROCHEMISTRY

Selection is more intelligent than design: improving the affinity of a bivalent ligand through directed evolution

(2012) Kareem M. Ahmad et al.   NUCLEIC ACIDS RESEARCH

Probing High Affinity Sequences of DNA Aptamer against VEGF165

(2012) Harleen Kaur et al.   PLoS One

Unique motifs and hydrophobic interactions shape the binding of modified DNA ligands to protein targets

(2012) D. R. Davies et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Towards a rational spacer design for bivalent inhibition of estrogen receptor

(2011) Alexander Bujotzek et al.   JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN

Aptamer-Based Multiplexed Proteomic Technology for Biomarker Discovery

(2011) Larry Gold et al.   PLoS One

Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

(2011) Keke Shao et al.   PLoS One

A streamlined protocol for emulsion polymerase chain reaction and subsequent purification

(2010) Tatjana Schütze et al.   ANALYTICAL BIOCHEMISTRY

Dual-aptamer-based delivery vehicle of doxorubicin to both PSMA (+) and PSMA (−) prostate cancers

(2010) Kyoungin Min et al.   BIOMATERIALS

Conservative secondary structure motif of streptavidin-binding aptamers generated by different laboratories

(2010) Tao Bing et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Selection of DNA aptamer against prostate specific antigen using a genetic algorithm and application to sensing

(2010) Nasa Savory et al.   BIOSENSORS & BIOELECTRONICS

Bioimaging of Nucleolin Aptamer-Containing 5-(N-benzylcarboxyamide)-2′-deoxyuridine More Capable of Specific Binding to Targets in Cancer Cells

(2010) Kyue Yim Lee et al.   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Expanding the Chemistry of DNA for in Vitro Selection

(2010) Jonathan D. Vaught et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Screening and Improvement of an Anti-VEGF DNA Aptamer

(2010) Yoshihiko Nonaka et al.   MOLECULES

A multivalent DNA aptamer specific for the B-cell receptor on human lymphoma and leukemia

(2010) Prabodhika R. Mallikaratchy et al.   NUCLEIC ACIDS RESEARCH

Aptamer evolution for array-based diagnostics

(2009) Mark Platt et al.   ANALYTICAL BIOCHEMISTRY

Aptamers as a model for functional evaluation of LNA and 2′-amino LNA

(2009) Frank J. Hernandez et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

An Aptamer-Based Bound/Free Separation System for Protein Detection

(2009) Mieko Fukasawa et al.   ELECTROANALYSIS

Analysis of a complete DNA-protein affinity landscape

(2009) W. Rowe et al.   Journal of the Royal Society Interface

Bivalent Ligands with Long Nanometer-Scale Flexible Linkers†

(2008) Ling Tian et al.   BIOCHEMISTRY

Selection of DNA aptamers against insulin and construction of an aptameric enzyme subunit for insulin sensing

(2008) Wataru Yoshida et al.   BIOSENSORS & BIOELECTRONICS

Selection of DNA aptamers against VEGF165 using a protein competitor and the aptamer blotting method

(2008) Hijiri Hasegawa et al.   BIOTECHNOLOGY LETTERS

Array-based evolution of DNA aptamers allows modelling of an explicit sequence-fitness landscape

(2008) Christopher G. Knight et al.   NUCLEIC ACIDS RESEARCH

Molecular assembly for high-performance bivalent nucleic acid inhibitor

(2008) Y. Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Improvement of Aptamer Affinity by Dimerization

(2008) Hijiri Hasegawa et al.   SENSORS