Journal
RNA BIOLOGY
Volume 13, Issue 12, Pages 1232-1245Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2016.1236173
Keywords
Aptamers; chemical antibodies; chemically-modified aptamers; in vitro selection; modified nucleotides; nucleic acid ligands; SELEX
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Funding
- McCusker Charitable Foundation
- Western Australian Neuroscience Research Institute
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Nucleic acid aptamers are single-stranded DNA or RNA oligonucleotide sequences that bind to a specific target molecule with high affinity and specificity through their ability to adopt 3-dimensional structure in solution. Aptamers have huge potential as targeted therapeutics, diagnostics, delivery agents and as biosensors. However, aptamers composed of natural nucleotide monomers are quickly degraded in vivo and show poor pharmacodynamic properties. To overcome this, chemically-modified nucleic acid aptamers are developed by incorporating modified nucleotides after or during the selection process by Systematic Evolution of Ligands by EXponential enrichment (SELEX). This review will discuss the development of chemically-modified aptamers and provide the pros and cons, and new insights on in vitro aptamer selection strategies by using chemically-modified nucleic acid libraries.
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