Recent advances in universal TA cloning methods for use in function studies

Generation and characterization of quinolone-specific DNA aptamers suitable for water monitoring

(2016) C. Reinemann et al.   BIOSENSORS & BIOELECTRONICS

Recombinant cloning strategies for protein expression

(2016) Patrick HN Celie et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

In vitro selection of RNA aptamers against CA125 tumor marker in ovarian cancer and its study by optical biosensing

(2016) Ilaria Lamberti et al.   METHODS

A Novel COL4A4 Mutation Identified in a Chinese Family with Thin Basement Membrane Nephropathy

(2016) Yan Xu et al.   Scientific Reports

Universal CG cloning of polymerase chain reaction products

(2015) Julian Stevenson et al.   ANALYTICAL BIOCHEMISTRY

Molecular cloning and expression analysis of theWEE1andCCS52Agenes in European pear (Pyrus communisL.) and their possible roles in a giant fruit mutant

(2015) T. Hanada et al.   JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY

Detection of Mycobacterium tuberculosis in extrapulmonary biopsy samples using PCR targeting IS6110, rpoB, and nested-rpoB PCR Cloning

(2015) Hossein Meghdadi et al.   Frontiers in Microbiology

Partially Overlapping Primer-Based PCR for Genome Walking

(2015) Haixing Li et al.   PLoS One

Development of SSR Markers and Genetic Diversity in White Birch (Betula platyphylla)

(2015) Wei Hao et al.   PLoS One

Effects of Methylation Status of CpG Sites within the HPV16 Long Control Region on HPV16-Positive Head and Neck Cancer Cells

(2015) Chunlin Zhang et al.   PLoS One

pKILLIN: a versatile positive-selection cloning vector based on the toxicity of Killin in Escherichia coli

(2014) Zelong Ma et al.   GENE

Development and characterization of microsatellite markers from an enriched genomic library of Saccharina japonica

(2014) Jing Zhang et al.   JOURNAL OF APPLIED PHYCOLOGY

Construction of a lentiviral T/A vector for direct analysis of PCR-amplified promoters

(2014) Fu-xian Yu et al.   MOLECULAR BIOLOGY REPORTS

A Novel Binary T-Vector with the GFP Reporter Gene for Promoter Characterization

(2014) Shu-Ye Jiang et al.   PLoS One

A simple and fast system for cloning influenza A virus gene segments into pHW2000- and pCAGGS-based vectors

(2013) Volker Czudai-Matwich et al.   ARCHIVES OF VIROLOGY

Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

(2013) Wataru Fujii et al.   PLoS One

A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics

(2013) Chuntao Wang et al.   PLoS One

pPCV, a versatile vector for cloning PCR products

(2013) Christiane R Janner et al.   SpringerPlus

A cloning vector employing a versatile β-glucosidase as an indicator for recombinant clones

(2012) Dea-Eun Cheong et al.   ANALYTICAL BIOCHEMISTRY

A genome walking strategy for the identification of nucleotide sequences adjacent to known regions

(2012) Hailong Wang et al.   BIOTECHNOLOGY LETTERS

SLiCE: a novel bacterial cell extract-based DNA cloning method

(2012) Yongwei Zhang et al.   NUCLEIC ACIDS RESEARCH

Improved method for constructing plant amiRNA vectors with blue–white screening and MAGIC

(2011) Hong Yan et al.   BIOTECHNOLOGY LETTERS

FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method

(2011) Chaokun Li et al.   BMC BIOTECHNOLOGY

Optimized protocols and plasmids for in vivo cloning in yeast

(2011) Ana A. Kitazono   GENE

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids

(2010) Anton Bryksin et al.   BIOTECHNIQUES

One-step, zero-background ligation-independent cloning intron-containing hairpin RNA constructs for RNAi in plants

(2010) Guoyong Xu et al.   NEW PHYTOLOGIST

An advanced blue–white screening method for construction of shRNA expression vectors

(2009) Ju Yang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

(2009) Jiayuan Quan et al.   PLoS One

A novel vector for direct cloning PCR fragments by positive selection based on the lethal barnase

(2008) Leiming You et al.   MOLECULAR BIOLOGY REPORTS

Characterization of the Regulatory Region of the Dopa Decarboxylase Gene in Medaka: An in vivo Green Fluorescent Protein Reporter Assay Combined with a Simple TA-Cloning Method

(2008) Kazuhiro E. Fujimori   MOLECULAR BIOTECHNOLOGY