Emerging Lab-on-a-Chip Approaches for Liquid Biopsy in Lung Cancer: Status in CTCs and ctDNA Research and Clinical Validation

Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries

(2021) Hyuna Sung et al.   CA-A CANCER JOURNAL FOR CLINICIANS

A Review of Circulating Tumour Cell Enrichment Technologies

(2021) Amelia J. Rushton et al.   Cancers

Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer

(2020) Jessica Sierra et al.   SENSORS

Continuous Microfluidic Purification of DNA Using Magnetophoresis

(2020) Ying Xu et al.   Micromachines

DNA Methylation-Based Testing in Liquid Biopsies as Detection and Prognostic Biomarkers for the Four Major Cancer Types

(2020) Vera Constâncio et al.   Cells

Current and future applications of liquid biopsy in nonsmall cell lung cancer from early to advanced stages

(2020) Nicolas Guibert et al.   European Respiratory Review

Microfluidic chip for multiple detection of miRNA biomarkers in breast cancer based on three-segment hybridization

(2020) Yakun Gao et al.   AIP Advances

A Review on Microdevices for Isolating Circulating Tumor Cells

(2020) Kin Lei   Micromachines

Magnetically driven microfluidics for isolation of circulating tumor cells

(2020) Laan Luo et al.   Cancer Medicine

3D Printed Microfluidic Devices for Solid-Phase Extraction and On-Chip Fluorescent Labeling of Preterm Birth Risk Biomarkers

(2020) Anna V. Bickham et al.   ANALYTICAL CHEMISTRY

Clinical relevance of blood-based ctDNA analysis: mutation detection and beyond

(2020) Laura Keller et al.   BRITISH JOURNAL OF CANCER

Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation

(2019) Phil Oberacker et al.   PLOS BIOLOGY

Isolation of circulating tumor cells in non-small-cell-lung-cancer patients using a multi-flow microfluidic channel

(2019) Jian Zhou et al.   Microsystems & Nanoengineering

PD-L1 Expression in Circulating Tumor Cells Increases during Radio(chemo)therapy and Indicates Poor Prognosis in Non-small Cell Lung Cancer

(2019) Yang Wang et al.   Scientific Reports

Prognostic value of KRAS/TP53/PIK3CA in non‑small cell lung cancer

(2019) Jiayi Zhao et al.   Oncology Letters

Role of PD-L1 Expression in Non-Small Cell Lung Cancer and Their Prognostic Significance according to Clinicopathological Factors and Diagnostic Markers

(2019) Konrad Pawelczyk et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Technologies for circulating tumor cell separation from whole blood

(2019) Petra Bankó et al.   Journal of Hematology & Oncology

Emerging therapies for small cell lung cancer

(2019) Sen Yang et al.   Journal of Hematology & Oncology

Recent advances in microfluidic methods in cancer liquid biopsy

(2019) Florina S. Iliescu et al.   Biomicrofluidics

DEP-on-a-Chip: Dielectrophoresis Applied to Microfluidic Platforms

(2019) Haoqing Zhang et al.   Micromachines

Determination of PD-L1 Expression in Circulating Tumor Cells of NSCLC Patients and Correlation with Response to PD-1/PD-L1 Inhibitors

(2019) Melanie Janning et al.   Cancers

Rare epidermal growth factor receptor (EGFR) mutations in non-small cell lung cancer

(2019) Peter T Harrison et al.   SEMINARS IN CANCER BIOLOGY

Real‐world outcomes of NSCLC patients receiving tissue or circulating tumor DNA‐guided osimertinib treatment

(2019) Po‐Lan Su et al.   Cancer Medicine

Subtyping Lung Cancer Using DNA Methylation in Liquid Biopsies

(2019) Nunes et al.   Journal of Clinical Medicine

Liquid biopsy for lung cancers: an update on recent developments

(2019) Alberto E. Revelo et al.   Annals of Translational Medicine

Spiral microfluidic devices for cell separation and sorting in bioprocesses

(2019) N. Herrmann et al.   Biomicrofluidics

An Integrated Microfluidic Chip and Its Clinical Application for Circulating Tumor Cell Isolation and Single‐Cell Analysis

(2019) Mingxin Xu et al.   CYTOMETRY PART A

Microfluidic Technologies for cfDNA Isolation and Analysis

(2019) Xu et al.   Micromachines

Early detection of the major male cancer types in blood-based liquid biopsies using a DNA methylation panel

(2019) Vera Constâncio et al.   Clinical Epigenetics

Liquid Biopsy in Non-Small Cell Lung Cancer: Highlights and Challenges

(2019) Erika Rijavec et al.   Cancers

NanoVelcro rare-cell assays for detection and characterization of circulating tumor cells

(2018) Yu Jen Jan et al.   ADVANCED DRUG DELIVERY REVIEWS

Circulating tumor cells count as a predictor of survival in lung cancer

(2018) Konstantinos Syrigos et al.   CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY

Cell-Free Plasma DNA-Guided Treatment With Osimertinib in Patients With Advanced EGFR -Mutated NSCLC

(2018) Anna Buder et al.   Journal of Thoracic Oncology

Longitudinal Cell-Free DNA Analysis in Patients with Small Cell Lung Cancer Reveals Dynamic Insights into Treatment Efficacy and Disease Relapse

(2018) Karinna Almodovar et al.   Journal of Thoracic Oncology

Fully automated, on-site isolation of cfDNA from whole blood for cancer therapy monitoring

(2018) Chi-Ju Kim et al.   LAB ON A CHIP

Feasibility and Safety of Intrathoracic Biopsy and Repeat Biopsy for Evaluation of Programmed Cell Death Ligand–1 Expression for Immunotherapy in Non–Small Cell Lung Cancer

(2018) Emily B. Tsai et al.   RADIOLOGY

A liquid biopsy in primary lung cancer

(2018) Kazue Yoneda et al.   SURGERY TODAY

Complications of CT-guided transthoracic lung biopsy

(2018) David Lang et al.   WIENER KLINISCHE WOCHENSCHRIFT

Fabricating and Characterizing the Microfluidic Solid Phase Extraction Module Coupling with Integrated ESI Emitters

(2018) Hangbin Tang et al.   Micromachines

Liquid Biopsy in Lung Cancer: Clinical Applications of Circulating Biomarkers (CTCs and ctDNA)

(2018) Minji Lim et al.   Micromachines

The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy

(2018) Arutha Kulasinghe et al.   Micromachines

Simple and Low-Cost Sampling of Cell-Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA

(2018) Choong Eun Jin et al.   Advanced Science

Facile profiling of molecular heterogeneity by microfluidic digital melt

(2018) Christine M. O’Keefe et al.   Science Advances

The Prognostic Role of Circulating Tumor Cells (CTCs) in Lung Cancer

(2018) Joanna Kapeleris et al.   Frontiers in Oncology

Current and future perspectives of liquid biopsies in genomics-driven oncology

(2018) Ellen Heitzer et al.   NATURE REVIEWS GENETICS

Size- and deformability-based isolation of circulating tumor cells with microfluidic chips and their applications in clinical studies

(2018) Hongmei Chen et al.   AIP Advances

Circulating tumor cells as a predictive biomarker in patients with small cell lung cancer undergoing chemotherapy

(2017) Charu Aggarwal et al.   LUNG CANCER

Digital Microfluidics for Nucleic Acid Amplification

(2017) Beatriz Coelho et al.   SENSORS

An integrated microfluidic chip for one-step isolation of circulating tumor cells

(2017) Tae Yoon Lee et al.   SENSORS AND ACTUATORS B-CHEMICAL

Targeting ALK: Precision Medicine Takes on Drug Resistance

(2017) Jessica J. Lin et al.   Cancer Discovery

Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection

(2017) Mo-Yuan Shen et al.   Advanced Healthcare Materials

Hyaluronic acid-functionalized electrospun PLGA nanofibers embedded in a microfluidic chip for cancer cell capture and culture

(2017) Gangwei Xu et al.   Biomaterials Science

Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics

(2017) Nasir Ali et al.   Biomed Research International

Microfluidic Platform for Cell Isolation and Manipulation Based on Cell Properties

(2017) Caffiyar Yousuff et al.   Micromachines

Lung Cancer: Understanding Its Molecular Pathology and the 2015 WHO Classification

(2017) Kentaro Inamura   Frontiers in Oncology

Microfluidic Isolation of Circulating Tumor Cell Clusters by Size and Asymmetry

(2017) Sam H. Au et al.   Scientific Reports

Optofluidic device for the quantification of circulating tumor cells in breast cancer

(2017) Eric Pedrol et al.   Scientific Reports

Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data

(2017) Sean C. Taylor et al.   Scientific Reports

Small cell lung cancer: model of circulating tumor cell tumorospheres in chemoresistance

(2017) Lukas Klameth et al.   Scientific Reports

Clinical evaluation of a novel microfluidic device for epitope-independent enrichment of circulating tumour cells in patients with small cell lung cancer

(2016) Jakub Chudziak et al.   ANALYST

Future microfluidic and nanofluidic modular platforms for nucleic acid liquid biopsy in precision medicine

(2016) Ana Egatz-Gomez et al.   Biomicrofluidics

Detection of Therapeutically Targetable Driver and Resistance Mutations in Lung Cancer Patients by Next-Generation Sequencing of Cell-Free Circulating Tumor DNA

(2016) J. C. Thompson et al.   CLINICAL CANCER RESEARCH

Hybrid microfluidics combined with active and passive approaches for continuous cell separation

(2016) Sheng Yan et al.   ELECTROPHORESIS

Association Between Plasma Genotyping and Outcomes of Treatment With Osimertinib (AZD9291) in Advanced Non–Small-Cell Lung Cancer

(2016) Geoffrey R. Oxnard et al.   JOURNAL OF CLINICAL ONCOLOGY

ctDNA Determination of EGFR Mutation Status in European and Japanese Patients with Advanced NSCLC: The ASSESS Study

(2016) Martin Reck et al.   Journal of Thoracic Oncology

Dual-patterned immunofiltration (DIF) device for the rapid efficient negative selection of heterogeneous circulating tumor cells

(2016) Jiyoon Bu et al.   LAB ON A CHIP

Osimertinib for pretreated EGFR Thr790Met-positive advanced non-small-cell lung cancer (AURA2): a multicentre, open-label, single-arm, phase 2 study

(2016) Glenwood Goss et al.   LANCET ONCOLOGY

Metastasis as an evolutionary process

(2016) S. Turajlic et al.   SCIENCE

Affinity Versus Label-Free Isolation of Circulating Tumor Cells: Who Wins?

(2016) Vasudha Murlidhar et al.   Small

Nanoelectromechanical Chip (NELMEC) Combination of Nanoelectronics and Microfluidics to Diagnose Epithelial and Mesenchymal Circulating Tumor Cells from Leukocytes

(2016) Seied Ali Hosseini et al.   Small

Circulating cell-free DNA as a prognostic and predictive biomarker in non-small cell lung cancer

(2016) Bo Ai et al.   Oncotarget

Classification of large circulating tumor cells isolated with ultra-high throughput microfluidic Vortex technology

(2016) James Che et al.   Oncotarget

Plasma EGFR T790M ctDNA status is associated with clinical outcome in advanced NSCLC patients with acquired EGFR-TKI resistance

(2016) D. Zheng et al.   Scientific Reports

Label-Free Isolation and mRNA Detection of Circulating Tumor Cells from Patients with Metastatic Lung Cancer for Disease Diagnosis and Monitoring Therapeutic Efficacy

(2015) Jidong Wang et al.   ANALYTICAL CHEMISTRY

Simultaneous isolation and detection of circulating tumor cells with a microfluidic silicon-nanowire-array integrated with magnetic upconversion nanoprobes

(2015) Chao Wang et al.   BIOMATERIALS

Cell-free DNA levels in plasma of patients with non-small-cell lung cancer and inflammatory lung disease

(2015) A Szpechcinski et al.   BRITISH JOURNAL OF CANCER

Enumeration and Molecular Characterization of Tumor Cells in Lung Cancer Patients Using a Novel In Vivo Device for Capturing Circulating Tumor Cells

(2015) T. M. Gorges et al.   CLINICAL CANCER RESEARCH

Detection of T790M, the Acquired Resistance EGFR Mutation, by Tumor Biopsy versus Noninvasive Blood-Based Analyses

(2015) T. K. Sundaresan et al.   CLINICAL CANCER RESEARCH

Small Cell Lung Cancer: Will Recent Progress Lead to Improved Outcomes?

(2015) M. C. Pietanza et al.   CLINICAL CANCER RESEARCH

Clinical value of chip-based digital-PCR platform for the detection of circulating DNA in metastatic colorectal cancer

(2015) David Sefrioui et al.   DIGESTIVE AND LIVER DISEASE

Size and deformability based separation of circulating tumor cells from castrate resistant prostate cancer patients using resettable cell traps

(2015) Xi Qin et al.   LAB ON A CHIP

The biology of circulating tumor cells

(2015) K Pantel et al.   ONCOGENE

Acoustic separation of circulating tumor cells

(2015) Peng Li et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Benchtop Technologies for Circulating Tumor Cells Separation Based on Biophysical Properties

(2015) Wan Shi Low et al.   Biomed Research International

CD133+ Cancer Stem-like Cells in Small Cell Lung Cancer Are Highly Tumorigenic and Chemoresistant but Sensitive to a Novel Neuropeptide Antagonist

(2014) S. Sarvi et al.   CANCER RESEARCH

Microfluidic, marker-free isolation of circulating tumor cells from blood samples

(2014) Nezihi Murat Karabacak et al.   Nature Protocols

Molecular analysis of circulating tumour cells—biology and biomarkers

(2014) Matthew G. Krebs et al.   Nature Reviews Clinical Oncology

Clinical Validation of an Ultra High-Throughput Spiral Microfluidics for the Detection and Enrichment of Viable Circulating Tumor Cells

(2014) Bee Luan Khoo et al.   PLoS One

Detection of Circulating Tumor DNA in Early- and Late-Stage Human Malignancies

(2014) C. Bettegowda et al.   Science Translational Medicine

Blood-Based Analyses of Cancer: Circulating Tumor Cells and Circulating Tumor DNA

(2014) D. A. Haber et al.   Cancer Discovery

Isolation of Circulating Tumor Cells by Dielectrophoresis

(2014) Peter Gascoyne et al.   Cancers

A Multicenter Pilot Study Examining the Role of Circulating Tumor Cells as a Blood-Based Tumor Marker in Patients with Extensive Small-Cell Lung Cancer

(2014) Chao H. Huang et al.   Frontiers in Oncology

Highly selective biomechanical separation of cancer cells from leukocytes using microfluidic ratchets and hydrodynamic concentrator

(2013) Bill K. Lin et al.   Biomicrofluidics

Separation of tumor cells with dielectrophoresis-based microfluidic chip

(2013) Mohammed Alshareef et al.   Biomicrofluidics

Technologies for label-free separation of circulating tumor cells: from historical foundations to recent developments

(2013) Chao Jin et al.   LAB ON A CHIP

A Microfluidic Liquid Phase Nucleic Acid Purification Chip to Selectively Isolate DNA or RNA from Low Copy/Single Bacterial Cells in Minute Sample Volume Followed by Direct On-Chip Quantitative PCR Assay

(2012) Rui Zhang et al.   ANALYTICAL CHEMISTRY

Integrative genome analyses identify key somatic driver mutations of small-cell lung cancer

(2012) Martin Peifer et al.   NATURE GENETICS

Evaluation and Prognostic Significance of Circulating Tumor Cells in Patients With Non–Small-Cell Lung Cancer

(2011) Matthew G. Krebs et al.   JOURNAL OF CLINICAL ONCOLOGY

Analysis of Circulating Tumor Cells in Patients with Non-small Cell Lung Cancer Using Epithelial Marker-Dependent and -Independent Approaches

(2011) Matthew G. Krebs et al.   Journal of Thoracic Oncology

Continuous separation of breast cancer cells from blood samples using multi-orifice flow fractionation (MOFF) and dielectrophoresis (DEP)

(2011) Hui-Sung Moon et al.   LAB ON A CHIP

Circulating tumour cells, their role in metastasis and their clinical utility in lung cancer

(2011) John D. O’Flaherty et al.   LUNG CANCER

A magnetic bead-based DNA extraction and purification microfluidic device

(2011) Sayyed Mohamad Azimi et al.   Microfluidics and Nanofluidics

An integrated microfluidic device for DNA purification and PCR amplification of STR fragments

(2009) Joan M. Bienvenue et al.   Forensic Science International-Genetics

Systemic Spread Is an Early Step in Breast Cancer

(2008) Yves Hüsemann et al.   CANCER CELL

Detection of Mutations inEGFRin Circulating Lung-Cancer Cells

(2008) Shyamala Maheswaran et al.   NEW ENGLAND JOURNAL OF MEDICINE

Electrophoretic separations on microfluidic chips

(2007) Dapeng Wu et al.   JOURNAL OF CHROMATOGRAPHY A