mRNA vaccines manufacturing: Challenges and bottlenecks

The challenge and prospect of mRNA therapeutics landscape

(2020) Yuhua Weng et al.   BIOTECHNOLOGY ADVANCES

Flexible Manufacturing: The Future State of Drug Product Development and Commercialization in the Pharmaceutical Industry

(2020) Yash Kapoor et al.   Journal of Pharmaceutical Innovation

Vaccine Hesitancy: A Growing Concern

(2020) Jonathan Kennedy   PEDIATRIC DRUGS

Mannosylated Poly(ethylene imine) Copolymers Enhance saRNA Uptake and Expression in Human Skin Explants

(2020) Anna K. Blakney et al.   BIOMACROMOLECULES

The COVID-19 vaccine development landscape

(2020) Tung Thanh Le et al.   NATURE REVIEWS DRUG DISCOVERY

An RNA vaccine drives immunity in checkpoint-inhibitor-treated melanoma

(2020) Ugur Sahin et al.   NATURE

An mRNA Vaccine against SARS-CoV-2 — Preliminary Report

(2020) Lisa A. Jackson et al.   NEW ENGLAND JOURNAL OF MEDICINE

Timeline: Vaccines

(2020) Rino Rappuoli   CELL

Safety and Immunogenicity of SARS-CoV-2 mRNA-1273 Vaccine in Older Adults

(2020) Evan J. Anderson et al.   NEW ENGLAND JOURNAL OF MEDICINE

Durability of Responses after SARS-CoV-2 mRNA-1273 Vaccination

(2020) Alicia T. Widge et al.   NEW ENGLAND JOURNAL OF MEDICINE

Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine

(2020) Fernando P. Polack et al.   NEW ENGLAND JOURNAL OF MEDICINE

Resources, Production Scales and Time Required for Producing RNA Vaccines for the Global Pandemic Demand

(2020) Zoltán Kis et al.   Vaccines

Turning the corner on therapeutic cancer vaccines

(2019) Robert E. Hollingsworth et al.   npj Vaccines

Intradermal delivery of modified mRNA encoding VEGF-A in patients with type 2 diabetes

(2019) Li-Ming Gan et al.   Nature Communications

Process mapping of vaccines: Understanding the limitations in current response to emerging epidemic threats

(2019) Georgina Drury et al.   VACCINE

Adoptive Transfer of NKG2D CAR mRNA-Engineered Natural Killer Cells in Colorectal Cancer Patients

(2019) Lin Xiao et al.   MOLECULAR THERAPY

mRNA as a Transformative Technology for Vaccine Development to Control Infectious Diseases

(2019) Giulietta Maruggi et al.   MOLECULAR THERAPY

Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery

(2019) Piotr S. Kowalski et al.   MOLECULAR THERAPY

A phase I/IIa study of the mRNA-based cancer immunotherapy CV9201 in patients with stage IIIB/IV non-small cell lung cancer

(2019) Martin Sebastian et al.   CANCER IMMUNOLOGY IMMUNOTHERAPY

Design of in vitro Transcribed mRNA Vectors for Research and Therapy

(2019) Marina Tusup et al.   CHIMIA

A phase I multicenter study to assess the safety, tolerability, and immunogenicity of mRNA-4157 alone in patients with resected solid tumors and in combination with pembrolizumab in patients with unresectable solid tumors.

(2019) Howard A. Burris et al.   JOURNAL OF CLINICAL ONCOLOGY

Immunogenicity and tolerability of personalized mRNA vaccine mRNA-4650 encoding defined neoantigens expressed by the autologous cancer.

(2019) Gal Cafri et al.   JOURNAL OF CLINICAL ONCOLOGY

mRNA vaccines against H10N8 and H7N9 influenza viruses of pandemic potential are immunogenic and well tolerated in healthy adults in phase 1 randomized clinical trials

(2019) Robert A. Feldman et al.   VACCINE

A Facile Method for the Removal of dsRNA Contaminant from In Vitro-Transcribed mRNA

(2019) Markus Baiersdörfer et al.   Molecular Therapy-Nucleic Acids

Emerging Technologies for Low‐Cost, Rapid Vaccine Manufacture

(2019) Zoltán Kis et al.   Biotechnology Journal

A trans-amplifying RNA vaccine strategy for induction of potent protective immunity

(2019) Tim Beissert et al.   MOLECULAR THERAPY

Advances in RNA Vaccines for Preventive Indications: A Case Study of A Vaccine Against Rabies

(2019) Armbruster et al.   Vaccines

Establishing Preferred Product Characterization for the Evaluation of RNA Vaccine Antigens

(2019) Cristina Poveda et al.   Vaccines

Tailoring mRNA Vaccine to Balance Innate/Adaptive Immune Response

(2019) Sergio Linares-Fernández et al.   TRENDS IN MOLECULAR MEDICINE

A guide to large-scale RNA sample preparation

(2018) Lorenzo Baronti et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Emergence of synthetic mRNA: In vitro synthesis of mRNA and its applications in regenerative medicine

(2018) Hyokyoung Kwon et al.   BIOMATERIALS

Development of Anti-Human Mesothelin-Targeted Chimeric Antigen Receptor Messenger RNA–Transfected Peripheral Blood Lymphocytes for Ovarian Cancer Therapy

(2018) Chien-Fu Hung et al.   HUMAN GENE THERAPY

Self-Amplifying RNA Vaccines Give Equivalent Protection against Influenza to mRNA Vaccines but at Much Lower Doses

(2018) Annette B. Vogel et al.   MOLECULAR THERAPY

mRNA vaccines — a new era in vaccinology

(2018) Norbert Pardi et al.   NATURE REVIEWS DRUG DISCOVERY

The broad socioeconomic benefits of vaccination

(2018) David E. Bloom et al.   Science Translational Medicine

mRNA polyplexes with post-conjugated GALA peptides efficiently target, transfect and activate antigen presenting cells

(2018) Bo Lou et al.   BIOCONJUGATE CHEMISTRY

mRNA transfection by a Xentry-protamine cell-penetrating peptide is enhanced by TLR antagonist E6446

(2018) Glenn D. Bell et al.   PLoS One

The Current Scientific and Regulatory Landscape in Advancing Integrated Continuous Biopharmaceutical Manufacturing

(2018) Adam C. Fisher et al.   TRENDS IN BIOTECHNOLOGY

New Vaccine Technologies to Combat Outbreak Situations

(2018) Susanne Rauch et al.   Frontiers in Immunology

Phase I clinical trial of an intranodally administered mRNA based therapeutic vaccine against HIV-1 infection

(2018) Lorna Leal et al.   AIDS

Multimodal Chromatography for Purification of Biotherapeutics – A Review

(2018) Vivek Halan et al.   CURRENT PROTEIN & PEPTIDE SCIENCE

Antimicrobial resistance and the role of vaccines

(2018) David E. Bloom et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Modified VEGF-A mRNA induces sustained multifaceted microvascular response and accelerates diabetic wound healing

(2018) Naidi Sun et al.   Scientific Reports

Poly(lactic acid) nanoparticles and cell-penetrating peptide potentiate mRNA-based vaccine expression in dendritic cells triggering their activation

(2018) Anne-Line Coolen et al.   BIOMATERIALS

Safety and immunogenicity of a mRNA rabies vaccine in healthy adults: an open-label, non-randomised, prospective, first-in-human phase 1 clinical trial

(2017) Martin Alberer et al.   LANCET

Preclinical and Clinical Demonstration of Immunogenicity by mRNA Vaccines against H10N8 and H7N9 Influenza Viruses

(2017) Kapil Bahl et al.   MOLECULAR THERAPY

Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination

(2017) Norbert Pardi et al.   NATURE

Arginine-Rich Peptide-Based mRNA Nanocomplexes Efficiently Instigate Cytotoxic T Cell Immunity Dependent on the Amphipathic Organization of the Peptide

(2017) Vimal K. Udhayakumar et al.   Advanced Healthcare Materials

Conscious coupling: The challenges and opportunities of cascading enzymatic microreactors

(2017) Pia Gruber et al.   Biotechnology Journal

Formation and purification of tailored liposomes for drug delivery using a module-based micro continuous-flow system

(2017) Nikolay Dimov et al.   Scientific Reports

Immunization of HIV-1-Infected Persons With Autologous Dendritic Cells Transfected With mRNA Encoding HIV-1 Gag and Nef

(2016) Rajesh T. Gandhi et al.   JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES

Enhanced intranasal delivery of mRNA vaccine by overcoming the nasal epithelial barrier via intra- and paracellular pathways

(2016) Man Li et al.   JOURNAL OF CONTROLLED RELEASE

Systemic RNA delivery to dendritic cells exploits antiviral defence for cancer immunotherapy

(2016) Lena M. Kranz et al.   NATURE

mRNA capping: biological functions and applications

(2016) Anand Ramanathan et al.   NUCLEIC ACIDS RESEARCH

A general method for rapid and cost-efficient large-scale production of 5′ capped RNA

(2016) Anna-Lisa Fuchs et al.   RNA

From the RNA world to the clinic

(2016) B. A. Sullenger et al.   SCIENCE

Concise Review: Application of In Vitro Transcribed Messenger RNA for Cellular Engineering and Reprogramming: Progress and Challenges

(2016) Heidrun Steinle et al.   STEM CELLS

Immune response and long-term clinical outcome in advanced melanoma patients vaccinated with tumor-mRNA-transfected dendritic cells

(2016) Jon Amund Kyte et al.   OncoImmunology

Induction of HIV-1 gag specific immune responses by cationic micelles mediated delivery of gag mRNA

(2015) Mengnan Zhao et al.   DRUG DELIVERY

The messenger's great message for vaccination

(2015) Steve Pascolo   Expert Review of Vaccines

The business impact of an integrated continuous biomanufacturing platform for recombinant protein production

(2015) Jason Walther et al.   JOURNAL OF BIOTECHNOLOGY

Messenger RNA-based therapeutics for the treatment of apoptosis-associated diseases

(2015) Akitsugu Matsui et al.   Scientific Reports

Self-adjuvanted mRNA vaccination in advanced prostate cancer patients: a first-in-man phase I/IIa study

(2015) Hubert Kübler et al.   Journal for ImmunoTherapy of Cancer

mRNA-based therapeutics — developing a new class of drugs

(2014) Ugur Sahin et al.   NATURE REVIEWS DRUG DISCOVERY

History of vaccination

(2014) S. Plotkin   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA: The new revolution in nucleic acid vaccines

(2013) Andrew J. Geall et al.   SEMINARS IN IMMUNOLOGY

Challenges and advances towards the rational design of mRNA vaccines

(2013) Charlotte Pollard et al.   TRENDS IN MOLECULAR MEDICINE

A novel, disruptive vaccination technology

(2013) Karl-Josef Kallen et al.   Human Vaccines & Immunotherapeutics

Mesothelin-Specific Chimeric Antigen Receptor mRNA-Engineered T Cells Induce Antitumor Activity in Solid Malignancies

(2013) G. L. Beatty et al.   Cancer Immunology Research

The current challenges for vaccine development

(2012) P. Oyston et al.   JOURNAL OF MEDICAL MICROBIOLOGY

Protective efficacy of in vitro synthesized, specific mRNA vaccines against influenza A virus infection

(2012) Benjamin Petsch et al.   NATURE BIOTECHNOLOGY

Developing mRNA-vaccine technologies

(2012) Thomas Schlake et al.   RNA Biology

A phase I/IIa immunotherapy trial of HIV-1-infected patients with Tat, Rev and Nef expressing dendritic cells followed by treatment interruption

(2011) Sabine D. Allard et al.   CLINICAL IMMUNOLOGY

Selective uptake of naked vaccine RNA by dendritic cells is driven by macropinocytosis and abrogated upon DC maturation

(2011) M Diken et al.   GENE THERAPY

The development of vaccines: how the past led to the future

(2011) Stanley A. Plotkin et al.   NATURE REVIEWS MICROBIOLOGY

Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

(2011) Katalin Karikó et al.   NUCLEIC ACIDS RESEARCH

Intranodal Vaccination with Naked Antigen-Encoding RNA Elicits Potent Prophylactic and Therapeutic Antitumoral Immunity

(2010) S. Kreiter et al.   CANCER RESEARCH

Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

(2010) L. E. Easton et al.   RNA

Direct Injection of Protamine-protected mRNA: Results of a Phase 1/2 Vaccination Trial in Metastatic Melanoma Patients

(2009) Benjamin Weide et al.   JOURNAL OF IMMUNOTHERAPY

Vaccination greatly reduces disease, disability, death and inequity worldwide

(2008) FE Andre et al.   BULLETIN OF THE WORLD HEALTH ORGANIZATION

Results of the First Phase I/II Clinical Vaccination Trial With Direct Injection of mRNA

(2008) Benjamin Weide et al.   JOURNAL OF IMMUNOTHERAPY