TSC1 binding to lysosomal PIPs is required for TSC complex translocation and mTORC1 regulation

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling

(2021) Mirja Tamara Prentzell et al.   CELL

Structural insights into TSC complex assembly and GAP activity on Rheb

(2021) Huirong Yang et al.   Nature Communications

Netrin-1 promotes naive pluripotency through Neo1 and Unc5b co-regulation of Wnt and MAPK signalling

(2020) Aurélia Huyghe et al.   NATURE CELL BIOLOGY

Structure of the TSC2 GAP Domain: Mechanistic Insight into Catalysis and Pathogenic Mutations

(2020) Patrick Hansmann et al.   STRUCTURE

Architecture of the Tuberous Sclerosis Protein Complex

(2020) Kailash Ramlaul et al.   JOURNAL OF MOLECULAR BIOLOGY

Comparison of the functional and structural characteristics of rare TSC2 variants with clinical and genetic findings

(2019) Luiz G. Dufner Almeida et al.   HUMAN MUTATION

Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence

(2018) Christopher H. Choy et al.   JOURNAL OF CELL SCIENCE

Genetically encoded lipid biosensors

(2018) Rachel C. Wills et al.   MOLECULAR BIOLOGY OF THE CELL

Apilimod, a candidate anticancer therapeutic, arrests not only PtdIns(3,5)P2 but also PtdIns5P synthesis by PIKfyve and induces bafilomycin A1-reversible aberrant endomembrane dilation

(2018) Diego Sbrissa et al.   PLoS One

The Proto-oncogene c-Kit Inhibits Tumor Growth by Behaving as a Dependence Receptor

(2018) Hong Wang et al.   MOLECULAR CELL

ATR/Chk1 signaling induces autophagy through sumoylated RhoB-mediated lysosomal translocation of TSC2 after DNA damage

(2018) Mingdong Liu et al.   Nature Communications

PtdIns3P controls mTORC1 signaling through lysosomal positioning

(2017) Zhi Hong et al.   JOURNAL OF CELL BIOLOGY

Mechanisms of mTORC1 activation by RHEB and inhibition by PRAS40

(2017) Haijuan Yang et al.   NATURE

CCBuilder 2.0: Powerful and accessible coiled-coil modeling

(2017) Christopher W. Wood et al.   PROTEIN SCIENCE

mTORC1 activity repression by late endosomal phosphatidylinositol 3,4-bisphosphate

(2017) Andrea L. Marat et al.   SCIENCE

ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions

(2017) D. Franke et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Structure of the Tuberous Sclerosis Complex 2 (TSC2) N Terminus Provides Insight into Complex Assembly and Tuberous Sclerosis Pathogenesis

(2016) Reinhard Zech et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Basis of the Interaction between Tuberous Sclerosis Complex 1 (TSC1) and Tre2-Bub2-Cdc16 Domain Family Member 7 (TBC1D7)

(2016) Jiayue Qin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of the TBC1D7–TSC1 complex reveals that TBC1D7 stabilizes dimerization of the TSC1 C-terminal coiled coil region

(2016) Zhongchao Gai et al.   Journal of Molecular Cell Biology

Lysosomal recruitment of TSC2 is a universal response to cellular stress

(2016) Constantinos Demetriades et al.   Nature Communications

Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity

(2016) Bernadette Carroll et al.   eLife

Tuberous sclerosis complex

(2016) Elizabeth P. Henske et al.   Nature Reviews Disease Primers

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines

(2015) Eric Kowarz et al.   Biotechnology Journal

The Phyre2 web portal for protein modeling, prediction and analysis

(2015) Lawrence A Kelley et al.   Nature Protocols

Differential regulation of mTORC1 by leucine and glutamine

(2015) J. L. Jewell et al.   SCIENCE

Regulation of mTORC1 by PI3K signaling

(2015) Christian C. Dibble et al.   TRENDS IN CELL BIOLOGY

Automated Pipeline for Purification, Biophysical and X-Ray Analysis of Biomacromolecular Solutions

(2015) Melissa A. Graewert et al.   Scientific Reports

Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines

(2015) Eric Kowarz et al.   Biotechnology Journal

Spatial Control of the TSC Complex Integrates Insulin and Nutrient Regulation of mTORC1 at the Lysosome

(2014) Suchithra Menon et al.   CELL

Regulation of TORC1 in Response to Amino Acid Starvation via Lysosomal Recruitment of TSC2

(2014) Constantinos Demetriades et al.   CELL

Ral and Rheb GTPase Activating Proteins Integrate mTOR and GTPase Signaling in Aging, Autophagy, and Tumor Cell Invasion

(2014) Timothy D. Martin et al.   MOLECULAR CELL

Making new contacts: the mTOR network in metabolism and signalling crosstalk

(2014) Mitsugu Shimobayashi et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Deciphering key features in protein structures with the new ENDscript server

(2014) Xavier Robert et al.   NUCLEIC ACIDS RESEARCH

Identification of Regions Critical for the Integrity of the TSC1-TSC2-TBC1D7 Complex

(2014) Arthur Jorge Santiago Lima et al.   PLoS One

PIKfyve, a Class III PI Kinase, Is the Target of the Small Molecular IL-12/IL-23 Inhibitor Apilimod and a Player in Toll-like Receptor Signaling

(2013) Xinming Cai et al.   CHEMISTRY & BIOLOGY

Crystal structure of the yeast TSC1 core domain and implications for tuberous sclerosis pathological mutations

(2013) Wei Sun et al.   Nature Communications

Towards automated crystallographic structure refinement withphenix.refine

(2012) Pavel V. Afonine et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

The TSC1-TSC2 complex consists of multiple TSC1 and TSC2 subunits

(2012) Marianne Hoogeveen-Westerveld et al.   BMC BIOCHEMISTRY

Functional Assessment ofTSC2 Variants Identified in Individuals with Tuberous Sclerosis Complex

(2012) Marianne Hoogeveen-Westerveld et al.   HUMAN MUTATION

XDSAPP: a graphical user interface for the convenient processing of diffraction data usingXDS

(2012) Michael Krug et al.   JOURNAL OF APPLIED CRYSTALLOGRAPHY

Facilities for macromolecular crystallography at the Helmholtz-Zentrum Berlin

(2012) Uwe Mueller et al.   JOURNAL OF SYNCHROTRON RADIATION

TBC1D7 Is a Third Subunit of the TSC1-TSC2 Complex Upstream of mTORC1

(2012) Christian C. Dibble et al.   MOLECULAR CELL

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

A practical guide to evaluating colocalization in biological microscopy

(2011) Kenneth W. Dunn et al.   AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY

Functional assessment of variants in the TSC1 and TSC2 genes identified in individuals with Tuberous Sclerosis Complex

(2011) Marianne Hoogeveen-Westerveld et al.   HUMAN MUTATION

Functional assessment of TSC1 missense variants identified in individuals with tuberous sclerosis complex

(2011) Marianne Hoogeveen-Westerveld et al.   HUMAN MUTATION

XDS

(2010) Wolfgang Kabsch   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Features and development ofCoot

(2010) P. Emsley et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

PHENIX: a comprehensive Python-based system for macromolecular structure solution

(2010) Paul D. Adams et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Decision-making in structure solution using Bayesian estimates of map quality: thePHENIX AutoSolwizard

(2009) Thomas C. Terwilliger et al.   ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY

Characterization of the Intrinsic and TSC2-GAP-Regulated GTPase Activity of Rheb by Real-Time NMR

(2009) C. B. Marshall et al.   Science Signaling

The Rap–RapGAP complex: GTP hydrolysis without catalytic glutamine and arginine residues

(2008) Andrea Scrima et al.   EMBO JOURNAL