Review
Biochemistry & Molecular Biology
Vera S. Ovechkina, Suren M. Zakian, Sergey P. Medvedev, Kamila R. Valetdinova
Summary: Genetically encoded fluorescent biosensors allow visualization of biological processes in real time, providing benefits such as monitoring drug action and screening at the single-cell resolution. Research aims to improve biomedical applications by exploring their potential and addressing challenges.
Review
Developmental Biology
Akinobu Nakamura, Yuhei Goto, Yohei Kondo, Kazuhiro Aoki
Summary: This review provides an overview of the use of genetically encoded fluorescent biosensors for visualizing ERK activity in live cells, as well as their applications in developmental studies of model organisms. It discusses the current understanding of how ERK dynamics are encoded and decoded for cell fate decision-making.
Article
Biochemistry & Molecular Biology
Salma Saeed Khan, Yi Shen, Muhammad Qaiser Fatmi, Robert E. Campbell, Habib Bokhari
Summary: Genetically encoded biosensors based on engineered fluorescent proteins play a key role in monitoring specific ions and molecules in biological systems, with the potential to detect highly toxic arsenic ions. The development of prototype arsenic biosensors using a bacterial transcription factor scaffold opens up possibilities for improved performance in detecting As3+ in various biological and environmental systems.
Review
Chemistry, Analytical
Cong Quang Vu, Satoshi Arai
Summary: Genetically encoded fluorescence lifetime biosensors provide a powerful tool for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic cellular processes. This review gives an overview of the principles, applications, and advancements in quantitative imaging with genetically encoded fluorescence lifetime biosensors using fluorescence lifetime imaging microscopy (go-FLIM), highlighting the distinct advantages of fluorescence lifetime-based measurements.
Article
Chemistry, Analytical
Ze Fan, Cai-Xia Dou, Li-Juan Tang, Fenglin Wang, Jian-Hui Jiang
Summary: Genetically encoded sensors with a modular design have been developed for ratiometric and multiplexed imaging of small molecules in live cells. The sensors use aptazyme as a recognition module and light-up RNA aptamer as a signal reporter. The design allows for quantitative imaging and detection of small molecules, and the versatility of the approach is demonstrated by replacing the aptazymes for imaging different molecules.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Namdoo Kim, Seunghan Shin, Se Won Bae
Summary: cAMP serves a crucial role in signal transduction pathways, with research focusing on its dynamics leading to insights for drug development and disease treatment. To enable real-time non-invasive imaging, genetically-encoded sensors based on fluorescent proteins and luciferases have been developed as powerful tools.
Review
Biochemistry & Molecular Biology
David Stellon, Jana Talbot, Alex W. W. Hewitt, Anna E. E. King, Anthony L. L. Cook
Summary: Neurodegenerative diseases lead to progressive loss of neuronal structure and function, causing cell death and irreversible brain atrophy. While the mechanisms of neurodegeneration are still unknown, genetically encodable fluorescent biosensors (GEFBs) offer a unique way to study disease mechanisms and identify new therapies when combined with induced pluripotent stem cells (iPSCs).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Rainer Waadt, Joerg Kudla, Hannes Kollist
Summary: Understanding plant physiology at the organismic scale involves analyzing biological processes down to the cellular and molecular levels. Fluorescent protein (FP) technology allows for direct monitoring of various cellular dynamics in plants, and multiplexing techniques enable simultaneous recording of multiple biological processes.
Review
Biotechnology & Applied Microbiology
Jian Zhang, Qingxiao Pang, Qi Wang, Qingsheng Qi, Qian Wang
Summary: Genetically encoded biosensors have a wide range of detectable signals and potential applications, with continuous improvement in performance and functions. Strategies for tuning biosensors and expanding input signals have been summarized. Analysis of different output signals and their practical requirements in biotechnology, biomedical applications, and environmental safety provides insight for improving design and optimization of biosensors in various application scenarios.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Xuanyu Wang, Kaili Zhu, Dongdong Chen, Juan Wang, Xiaofei Wang, An Xu, Lijun Wu, Luzhi Li, Shaopeng Chen
Summary: This study successfully established a genetically encoded biosensor in vitro for arsenic detection using ArsR and GFP genes. An evolved ArsR mutant, ep3, was found to respond to arsenic and regulate GFP expression. A standard procedure for in vitro arsenic detection was established based on detailed experimental analysis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Maryna Loewe, Sebastian Haensch, Eymen Hachani, Lutz Schmitt, Stefanie Weidtkamp-Peters, Alexej Kedrov
Summary: Biochemical processes in crowded cellular environments, such as membranes, are affected by macromolecular crowding. Researchers developed a fluorescence-based sensor to quantify macromolecular crowding at membrane interfaces, and validated its dynamics in Escherichia coli inner membrane vesicles.
Review
Cell Biology
Donnell White, Qinglin Yang
Summary: ATP is the primary molecule for storing and transferring energy in cells, produced by mitochondria and cytosolic glycolysis. Mitochondria produce most cellular ATP under normal oxygen conditions, while cytosolic glycolysis plays a major role in proliferating cells or hypoxic conditions. Under pathological conditions, ATP demand increases, affecting the function and communication of mitochondria and cytosolic glycolysis.
Article
Chemistry, Multidisciplinary
Arunika Ekanayake, Lena Sobze, Payam Kelich, Jihea Youk, Nicholas J. Bennett, Raja Mukherjee, Atul Bhardwaj, Frank Wuest, Lela Vukovic, Ratmir Derda
Summary: This study presents a novel late-stage approach to convert peptide libraries into diverse macrocyclic libraries, encoding amino acid sequences and pharmacophores through DNA, significantly enhancing the value of genetically encoded phage libraries in molecular discoveries.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Kamila R. Valetdinova, Tuyana B. Malankhanova, Suren M. Zakian, Sergey P. Medvedev
Summary: The development of cell models of human diseases using iPSCs has driven personalized regenerative medicine, and combining biosensor technology with iPSCs can optimize drug screening and research.
Review
Chemistry, Multidisciplinary
Minji Wang, Yifan Da, Yang Tian
Summary: Genetically encoded fluorescent sensors are powerful tools for visualizing physiological processes in living cells and animals. This study reviews the history and structural basis of fluorescent proteins and discusses the design of genetically encoded biosensors. Several major types of genetically encoded biosensors currently used are also briefly reviewed, providing insight for future fluorescent biosensor design.
CHEMICAL SOCIETY REVIEWS
(2023)
Editorial Material
Cell Biology
Gerald R. V. Hammond
JOURNAL OF CELL BIOLOGY
(2018)
Article
Cell Biology
Gerald R. Hammond, Yang Hong
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY
(2018)
Article
Cell Biology
Mira Sohn, Marek Korzeniowski, James P. Zewe, Rachel C. Wills, Gerald R. V. Hammond, Jana Humpolickova, Lukas Vrzal, Dominika Chalupska, Vaclav Veverka, Gregory D. Fairn, Evzen Boura, Tamas Balla
JOURNAL OF CELL BIOLOGY
(2018)
Article
Multidisciplinary Sciences
Krishna Chintaluri, Brady D. Goulden, Camilyn Celmenza, Golam Saffi, Emily Miraglia, Gerald R. Hammond, Roberto J. Botelho
Correction
Multidisciplinary Sciences
Krishna Chintaluri, Brady D. Goulden, Camilyn Clemenza, Golam Saffi, Emily Miraglia, Gerald R. V. Hammond, Roberto J. Botelho
Article
Cell Biology
Brady D. Goulden, Jonathan Pacheco, Allyson Dull, James P. Zewe, Alexander Deiters, Gerald R. Hammond
JOURNAL OF CELL BIOLOGY
(2019)
Editorial Material
Cell Biology
Gerald R. V. Hammond, Jonathan Pacheco
TRENDS IN CELL BIOLOGY
(2019)
Article
Cell Biology
James P. Zewe, April M. Miller, Sahana Sangappa, Rachel C. Wills, Brady D. Goulden, Gerald R. Hammond
JOURNAL OF CELL BIOLOGY
(2020)
Review
Cell Biology
Gerald R. V. Hammond, John E. Burke
CURRENT OPINION IN CELL BIOLOGY
(2020)
Article
Cell Biology
Wei Dong, Juan Lu, Xuejing Zhang, Yan Wu, Kaela Lettieri, Gerald R. Hammond, Yang Hong
JOURNAL OF CELL BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Dennis Vettkoetter, Martin Schneider, Brady D. Goulden, Holger Dill, Jana Liewald, Sandra Zeiler, Julia Guldan, Yilmaz Arda Ates, Shigeki Watanabe, Alexander Gottschalk
Summary: This study utilizes the light-evoked homo-oligomerization of CRY2 to silence synaptic transmission by clustering synaptic vesicles, providing a tool for acute silencing of specific neurons. The optoSynC tool demonstrates fast activation and reversibility, inhibiting exocytosis for hours at low light intensities without affecting ion currents or synaptic proteins. It has the potential for manipulating different synaptic vesicle pools and their transfer.
NATURE COMMUNICATIONS
(2022)