Building consensus on definition and nomenclature of hepatic, pancreatic, and biliary organoids

The Organoid Cell Atlas

(2021) Christoph Bock et al.   NATURE BIOTECHNOLOGY

Cholangiocyte organoids can repair bile ducts after transplantation in the human liver

(2021) Fotios Sampaziotis et al.   SCIENCE

Human Bile Contains Cholangiocyte Organoid-Initiating Cells Which Expand as Functional Cholangiocytes in Non-canonical Wnt Stimulating Conditions

(2021) Floris J. M. Roos et al.   Frontiers in Cell and Developmental Biology

Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids

(2020) Nikitas Georgakopoulos et al.   BMC DEVELOPMENTAL BIOLOGY

Organoid models of gastrointestinal cancers in basic and translational research

(2020) Harry Cheuk Hay Lau et al.   Nature Reviews Gastroenterology & Hepatology

Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors

(2020) Daisong Wang et al.   CELL

Regional differences in human biliary tissues and corresponding in vitro derived organoids

(2020) Casey A Rimland et al.   HEPATOLOGY

A Chemically Defined Hydrogel for Human Liver Organoid Culture

(2020) Shicheng Ye et al.   ADVANCED FUNCTIONAL MATERIALS

Current concepts in tumour-derived organoids

(2020) Ross J. Porter et al.   BRITISH JOURNAL OF CANCER

Human Pluripotent Stem Cell-Derived Organoids as Models of Liver Disease

(2020) Muhammad Nadzim Bin Ramli et al.   GASTROENTEROLOGY

Cholangiocarcinoma 2020: the next horizon in mechanisms and management

(2020) Jesus M. Banales et al.   Nature Reviews Gastroenterology & Hepatology

Human organoids: model systems for human biology and medicine

(2020) Jihoon Kim et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Mechano-modulatory synthetic niches for liver organoid derivation

(2020) Giovanni Sorrentino et al.   Nature Communications

High-Fidelity Drug Induced Liver Injury Screen Using Human PSC-derived Organoids

(2020) Tadahiro Shinozawa et al.   GASTROENTEROLOGY

Homeostatic mini-intestines through scaffold-guided organoid morphogenesis

(2020) Mikhail Nikolaev et al.   NATURE

Adaptable haemodynamic endothelial cells for organogenesis and tumorigenesis

(2020) Brisa Palikuqi et al.   NATURE

Immune-evasive human islet-like organoids ameliorate diabetes

(2020) Eiji Yoshihara et al.   NATURE

A Human Organoid Model of Aggressive Hepatoblastoma for Disease Modeling and Drug Testing

(2020) James A. Saltsman et al.   Cancers

Establishment of human fetal hepatocyte organoids and CRISPR–Cas9-based gene knockin and knockout in organoid cultures from human liver

(2020) Delilah Hendriks et al.   Nature Protocols

Human extrahepatic and intrahepatic cholangiocyte organoids show region-specific differentiation potential and model cystic fibrosis-related bile duct disease

(2020) Monique M. A. Verstegen et al.   Scientific Reports

Generation of hepato-biliary organoids from human induced pluripotent stem cells

(2019) Fenfang Wu et al.   JOURNAL OF HEPATOLOGY

Reactive cholangiocytes differentiate into proliferative hepatocytes with efficient DNA repair in mice with chronic liver injury

(2019) Rita Manco et al.   JOURNAL OF HEPATOLOGY

Kidney micro-organoids in suspension culture as a scalable source of human pluripotent stem cell-derived kidney cells

(2019) Santhosh V. Kumar et al.   DEVELOPMENT

Targeted Disruption of HLA Genes via CRISPR-Cas9 Generates iPSCs with Enhanced Immune Compatibility

(2019) Huaigeng Xu et al.   Cell Stem Cell

YAP, but Not RSPO-LGR4/5, Signaling in Biliary Epithelial Cells Promotes a Ductular Reaction in Response to Liver Injury

(2019) Lara Planas-Paz et al.   Cell Stem Cell

Lgr5+ stem/progenitor cells reside at the apex of a heterogeneous embryonic hepatoblast pool

(2019) Nicole Prior et al.   DEVELOPMENT

Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue

(2019) Olivia C. Tysoe et al.   Nature Protocols

Establishment of Patient-Derived Organoids and Drug Screening for Biliary Tract Carcinoma

(2019) Yoshimasa Saito et al.   Cell Reports

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids

(2019) Rie Ouchi et al.   Cell Metabolism

Liver organoids: from basic research to therapeutic applications

(2019) Nicole Prior et al.   GUT

Generation of expandable human pluripotent stem cell-derived hepatocyte-like liver organoids

(2019) Seon Ju Mun et al.   JOURNAL OF HEPATOLOGY

A human liver cell atlas reveals heterogeneity and epithelial progenitors

(2019) Nadim Aizarani et al.   NATURE

Modelling human hepato-biliary-pancreatic organogenesis from the foregut–midgut boundary

(2019) Hiroyuki Koike et al.   NATURE

Human ESC-derived expandable hepatic organoids enable therapeutic liver repopulation and pathophysiological modeling of alcoholic liver injury

(2019) Shuyong Wang et al.   CELL RESEARCH

Large‐scale Production of LGR5‐positive Bipotential Human Liver Stem Cells

(2019) Kerstin Schneeberger et al.   HEPATOLOGY

Epigenetic remodelling licences adult cholangiocytes for organoid formation and liver regeneration

(2019) Luigi Aloia et al.   NATURE CELL BIOLOGY

Robust, Long-Term Culture of Endoderm-Derived Hepatic Organoids for Disease Modeling

(2019) Soheil Akbari et al.   Stem Cell Reports

AXIN2+ Pericentral Hepatocytes Have Limited Contributions to Liver Homeostasis and Regeneration

(2019) Tianliang Sun et al.   Cell Stem Cell

Extracellular matrix hydrogel derived from decellularized tissues enables endodermal organoid culture

(2019) Giovanni Giuseppe Giobbe et al.   Nature Communications

Mutual Zonated Interactions of Wnt and Hh Signaling Are Orchestrating the Metabolism of the Adult Liver in Mice and Human

(2019) Erik Kolbe et al.   Cell Reports

Human Pancreatic Tumor Organoids Reveal Loss of Stem Cell Niche Factor Dependence during Disease Progression

(2018) Takashi Seino et al.   Cell Stem Cell

Chronic Liver Injury Induces Conversion of Biliary Epithelial Cells into Hepatocytes

(2018) Xing Deng et al.   Cell Stem Cell

MIR21 Drives Resistance to Heat Shock Protein 90 Inhibition in Cholangiocarcinoma

(2018) Andrea Lampis et al.   GASTROENTEROLOGY

Liver and pancreas: do similar embryonic development and tissue organization lead to similar mechanisms of tumorigenesis?

(2018) Elsa Ghurburrun et al.   GENE EXPRESSION

Self-assembled liver organoids recapitulate hepatobiliary organogenesis in vitro

(2018) Dipen Vyas et al.   HEPATOLOGY

Development of the liver: Insights into organ and tissue morphogenesis

(2018) Elke A. Ober et al.   JOURNAL OF HEPATOLOGY

Hepatocellular carcinoma

(2018) Alejandro Forner et al.   LANCET

Recent Advances in Lgr5 + Stem Cell Research

(2018) Carly Leung et al.   TRENDS IN CELL BIOLOGY

Use of bioreactors for culturing human retinal organoids improves photoreceptor yields

(2018) Patrick Ovando-Roche et al.   Stem Cell Research & Therapy

Expansion of Adult Human Pancreatic Tissue Yields Organoids Harboring Progenitor Cells with Endocrine Differentiation Potential

(2018) Cindy J.M. Loomans et al.   Stem Cell Reports

A Simple Bioreactor-Based Method to Generate Kidney Organoids from Pluripotent Stem Cells

(2018) Aneta Przepiorski et al.   Stem Cell Reports

Growth of Epithelial Organoids in a Defined Hydrogel

(2018) Nicolas Broguiere et al.   ADVANCED MATERIALS

De novo formation of the biliary system by TGFβ-mediated hepatocyte transdifferentiation

(2018) Johanna R. Schaub et al.   NATURE

Organoid Models of Human Liver Cancers Derived from Tumor Needle Biopsies

(2018) Sandro Nuciforo et al.   Cell Reports

Long-Term Expansion of Functional Mouse and Human Hepatocytes as 3D Organoids

(2018) Huili Hu et al.   CELL

Inflammatory Cytokine TNFα Promotes the Long-Term Expansion of Primary Hepatocytes in 3D Culture

(2018) Weng Chuan Peng et al.   CELL

In Vitro Expansion of Primary Human Hepatocytes with Efficient Liver Repopulation Capacity

(2018) Kun Zhang et al.   Cell Stem Cell

Bile-derived organoids from patients with primary sclerosing cholangitis recapitulate their inflammatory immune profile

(2018) Carol J. Soroka et al.   HEPATOLOGY

Advances in multicellular spheroids formation

(2017) X. Cui et al.   Journal of the Royal Society Interface

Cholangiocytes act as facultative liver stem cells during impaired hepatocyte regeneration

(2017) Alexander Raven et al.   NATURE

Human primary liver cancer–derived organoid cultures for disease modeling and drug screening

(2017) Laura Broutier et al.   NATURE MEDICINE

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids

(2017) Fotios Sampaziotis et al.   NATURE MEDICINE

Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells

(2017) Takanori Takebe et al.   Cell Reports

Single-Cell Analysis Uncovers Clonal Acinar Cell Heterogeneity in the Adult Pancreas

(2016) Damian Wollny et al.   DEVELOPMENTAL CELL

R‐spondin 1 and noggin facilitate expansion of resident stem cells from non‐damaged gallbladders

(2016) Natalia Lugli et al.   EMBO REPORTS

Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling

(2016) Meike Hohwieler et al.   GUT

Organoids: A historical perspective of thinking in three dimensions

(2016) Marina Simian et al.   JOURNAL OF CELL BIOLOGY

Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation

(2016) Laura Broutier et al.   Nature Protocols

CRISPR-Cas9 for in vivo Gene Therapy: Promise and Hurdles

(2016) Wei-Jing Dai et al.   Molecular Therapy-Nucleic Acids

A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure

(2016) Maayan Baron et al.   Cell Systems

Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease

(2016) Catherine C. Bell et al.   Scientific Reports

Zonation of hepatic fatty acid metabolism — The diversity of its regulation and the benefit of modeling

(2015) J. Schleicher et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver

(2015) Meritxell Huch et al.   CELL

Prospective Derivation of a Living Organoid Biobank of Colorectal Cancer Patients

(2015) Marc van de Wetering et al.   CELL

Organoid Models of Human and Mouse Ductal Pancreatic Cancer

(2015) Sylvia F. Boj et al.   CELL

Vascularized and Complex Organ Buds from Diverse Tissues via Mesenchymal Cell-Driven Condensation

(2015) Takanori Takebe et al.   Cell Stem Cell

Modeling mouse and human development using organoid cultures

(2015) M. Huch et al.   DEVELOPMENT

Bioengineering Thymus Organoids to Restore Thymic Function and Induce Donor-Specific Immune Tolerance to Allografts

(2015) Yong Fan et al.   MOLECULAR THERAPY

Self-renewing diploid Axin2+ cells fuel homeostatic renewal of the liver

(2015) Bruce Wang et al.   NATURE

Directed differentiation of cholangiocytes from human pluripotent stem cells

(2015) Mina Ogawa et al.   NATURE BIOTECHNOLOGY

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation

(2015) Fotios Sampaziotis et al.   NATURE BIOTECHNOLOGY

Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

(2015) Ling Huang et al.   NATURE MEDICINE

Ex Vivo Expansion and Differentiation of Human and Mouse Fetal Pancreatic Progenitors Are Modulated by Epidermal Growth Factor

(2015) Paola Bonfanti et al.   STEM CELLS AND DEVELOPMENT

Pancreatic Adenocarcinoma

(2015) David P. Ryan et al.   NEW ENGLAND JOURNAL OF MEDICINE

Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells

(2014) Noushin Dianat et al.   HEPATOLOGY

Three-dimensional organotypic culture: experimental models of mammalian biology and disease

(2014) Eliah R. Shamir et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Organogenesis in a dish: Modeling development and disease using organoid technologies

(2014) M. A. Lancaster et al.   SCIENCE

The organoid-initiating cells in mouse pancreas and liver are phenotypically and functionally similar

(2014) Craig Dorrell et al.   Stem Cell Research

Combined Hepatocellular and Cholangiocarcinoma (Biphenotypic) Tumors: Imaging Features and Diagnostic Accuracy of Contrast-Enhanced CT and MRI

(2013) Kathryn J. Fowler et al.   AMERICAN JOURNAL OF ROENTGENOLOGY

Artificial three-dimensional niches deconstruct pancreas development in vitro

(2013) C. Greggio et al.   DEVELOPMENT

Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis

(2013) Meritxell Huch et al.   EMBO JOURNAL

In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration

(2013) Meritxell Huch et al.   NATURE

Vascularized and functional human liver from an iPSC-derived organ bud transplant

(2013) Takanori Takebe et al.   NATURE

Production of hepatocyte-like cells from human pluripotent stem cells

(2013) Nicholas R F Hannan et al.   Nature Protocols

Spheroid culture as a tool for creating 3D complex tissues

(2013) Eelco Fennema et al.   TRENDS IN BIOTECHNOLOGY

Adult mammalian stem cells: the role of Wnt, Lgr5 and R-spondins

(2012) Jurian Schuijers et al.   EMBO JOURNAL

Advances in the formation, use and understanding of multi-cellular spheroids

(2012) Toni-Marie Achilli et al.   EXPERT OPINION ON BIOLOGICAL THERAPY

Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein

(2012) Amy P Wong et al.   NATURE BIOTECHNOLOGY

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Rapid generation of mature hepatocyte-like cells from human induced pluripotent stem cells by an efficient three-step protocol

(2011) Yu-Fan Chen et al.   HEPATOLOGY

Targeted gene correction of α1-antitrypsin deficiency in induced pluripotent stem cells

(2011) Kosuke Yusa et al.   NATURE

Basement Membrane Matrix (BME) has Multiple Uses with Stem Cells

(2011) Irina Arnaoutova et al.   Stem Cell Reviews and Reports

Perspectives on Tissue Interactions in Development and Disease

(2010) D. Strand et al.   CURRENT MOLECULAR MEDICINE

Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro

(2010) Jason R. Spence et al.   NATURE

Matrigel: A complex protein mixture required for optimal growth of cell culture

(2010) Chris S. Hughes et al.   PROTEOMICS

Wnt Signaling, Lgr5, and Stem Cells in the Intestine and Skin

(2009) Andrea Haegebarth et al.   AMERICAN JOURNAL OF PATHOLOGY

Sox17 Regulates Organ Lineage Segregation of Ventral Foregut Progenitor Cells

(2009) Jason R. Spence et al.   DEVELOPMENTAL CELL

Ethical Issues in Stem Cell Research

(2009) Bernard Lo et al.   ENDOCRINE REVIEWS

Highly efficient generation of human hepatocyte-like cells from induced pluripotent stem cells

(2009) Karim Si-Tayeb et al.   HEPATOLOGY

Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche

(2009) Toshiro Sato et al.   NATURE

Sustained in vitro intestinal epithelial culture within a Wnt-dependent stem cell niche

(2009) Akifumi Ootani et al.   NATURE MEDICINE

Stepwise differentiation of pluripotent stem cells into retinal cells

(2009) Fumitaka Osakada et al.   Nature Protocols

The cAMP effectors Epac and protein kinase a (PKA) are involved in the hepatic cystogenesis of an animal model of autosomal recessive polycystic kidney disease (ARPKD)

(2008) Jesús M. Banales et al.   HEPATOLOGY

MicroRNA15a modulates expression of the cell-cycle regulator Cdc25A and affects hepatic cystogenesis in a rat model of polycystic kidney disease

(2008) Seung-Ok Lee et al.   JOURNAL OF CLINICAL INVESTIGATION