Bacterial CRISPR screens for gene function

Comprehensive Genome-wide Perturbations via CRISPR Adaptation Reveal Complex Genetics of Antibiotic Sensitivity

(2020) Wenyan Jiang et al.   CELL

Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs

(2020) Marco Jost et al.   NATURE BIOTECHNOLOGY

A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus

(2020) Mathilde Guzzo et al.   mBio

CRISPR Tools To Control Gene Expression in Bacteria

(2020) Antoine Vigouroux et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria

(2020) Alicia Calvo-Villamañán et al.   NUCLEIC ACIDS RESEARCH

Effective CRISPRa-mediated control of gene expression in bacteria must overcome strict target site requirements

(2020) Jason Fontana et al.   Nature Communications

Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

(2020) Lun Yao et al.   Nature Communications

Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function

(2020) Robert C. Shields et al.   PLoS Pathogens

A Dual-Mechanism Antibiotic Kills Gram-Negative Bacteria and Avoids Drug Resistance

(2020) James K. Martin et al.   CELL

Programmable CRISPR‐Cas transcriptional activation in bacteria

(2020) Hsing‐I Ho et al.   Molecular Systems Biology

CRISPR–Cas9, CRISPRi and CRISPR-BEST-mediated genetic manipulation in streptomycetes

(2020) Yaojun Tong et al.   Nature Protocols

A High-efficacy CRISPRi System for Gene Function Discovery in Zymomonas mobilis

(2020) Amy B. Banta et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm Genetic Requirements in Enterococcus faecalis

(2020) Irina Afonina et al.   mBio

High-throughput mapping of the phage resistance landscape in E. coli

(2020) Vivek K. Mutalik et al.   PLOS BIOLOGY

Mismatch-CRISPRi Reveals the Co-varying Expression-Fitness Relationships of Essential Genes in Escherichia coli and Bacillus subtilis

(2020) John S. Hawkins et al.   Cell Systems

CRISPR Interference for Rapid Knockdown of Essential Cell Cycle Genes in Lactobacillus plantarum

(2019) Ine Storaker Myrbråten et al.   mSphere

Genome-Scale CRISPRa Screen Identifies Novel Factors for Cellular Reprogramming

(2019) Jian Yang et al.   Stem Cell Reports

A xylose-inducible expression system and a CRISPRi-plasmid for targeted knock-down of gene expression in Clostridioides difficile

(2019) Ute Müh et al.   JOURNAL OF BACTERIOLOGY

Functional genomics of the rapidly replicating bacterium Vibrio natriegens by CRISPRi

(2019) Henry H. Lee et al.   Nature Microbiology

Analysis of lipoprotein transport depletion in Vibrio cholerae using CRISPRi

(2019) Florence Caro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Versatile transcription control based on reversible dCas9 binding

(2019) Julia R Widom et al.   RNA

Modulating pathogenesis with Mobile-CRISPRi

(2019) Jiuxin Qu et al.   JOURNAL OF BACTERIOLOGY

Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST

(2019) Yaojun Tong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Broad-Host-Range CRISPRi Toolkit for Silencing Gene Expression in Burkholderia

(2019) Andrew M. Hogan et al.   ACS Synthetic Biology

Time-resolved imaging-based CRISPRi screening

(2019) Daniel Camsund et al.   NATURE METHODS

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

A Robust CRISPR Interference Gene Repression System in Pseudomonas

(2018) Sue Zanne Tan et al.   JOURNAL OF BACTERIOLOGY

Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

(2018) Jun Sun et al.   Microbial Cell Factories

CRISPR/dCas9-mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus

(2018) Ran Peng et al.   Microbial Cell Factories

Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

(2018) Antoine Vigouroux et al.   Molecular Systems Biology

Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference

(2018) Yusuke Sato’o et al.   PLoS One

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

(2018) Lun Cui et al.   Nature Communications

Single plasmid systems for inducible dual protein expression and for CRISPR-Cas9/CRISPRi gene regulation in lactic acid bacterium Lactococcus lactis

(2018) Aleš Berlec et al.   Scientific Reports

High-Level dCas9 Expression Induces Abnormal Cell Morphology in Escherichia coli

(2018) Suhyung Cho et al.   ACS Synthetic Biology

Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

(2018) Jason Fontana et al.   Biotechnology Journal

Mutant phenotypes for thousands of bacterial genes of unknown function

(2018) Morgan N. Price et al.   NATURE

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

(2018) François Rousset et al.   PLoS Genetics

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi

(2018) Jason M. Peters et al.   Nature Microbiology

Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent

(2017) Marco Jost et al.   MOLECULAR CELL

High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae

(2017) Xue Liu et al.   Molecular Systems Biology

High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding

(2017) Evan A. Boyle et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform

(2017) Jeremy M. Rock et al.   Nature Microbiology

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

A global genetic interaction network maps a wiring diagram of cellular function

(2016) M. Costanzo et al.   SCIENCE

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

Bacterial CRISPR: accomplishments and prospects

(2015) Jason M Peters et al.   CURRENT OPINION IN MICROBIOLOGY

Programming a Human Commensal Bacterium, Bacteroides thetaiotaomicron, to Sense and Respond to Stimuli in the Murine Gut Microbiota

(2015) Mark Mimee et al.   Cell Systems

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE