Pooled CRISPRi screening of the cyanobacterium Synechocystis sp PCC 6803 for enhanced industrial phenotypes

On the use of oxygenic photosynthesis for the sustainable production of commodity chemicals

(2019) Adam A. Pérez et al.   PHYSIOLOGIA PLANTARUM

Quantitative insights into the cyanobacterial cell economy

(2019) Tomáš Zavřel et al.   eLife

Economic feasibility and long-term sustainability criteria on the path to enable a transition from fossil fuels to biofuels

(2019) Giorgio Perin et al.   CURRENT OPINION IN BIOTECHNOLOGY

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium

(2019) Inés C. Cañadas et al.   ACS Synthetic Biology

Redirecting photosynthetic electron flux in the cyanobacterium Synechocystis sp. PCC 6803 by the deletion of flavodiiron protein Flv3

(2019) Kati Thiel et al.   Microbial Cell Factories

Proteome Mapping of a Cyanobacterium Reveals Distinct Compartment Organization and Cell-Dispersed Metabolism

(2019) Laura L. Baers et al.   PLANT PHYSIOLOGY

Elucidation of photoautotrophic carbon flux topology in Synechocystis PCC 6803 using genome-scale carbon mapping models

(2018) Saratram Gopalakrishnan et al.   METABOLIC ENGINEERING

Light-optimized growth of cyanobacterial cultures: Growth phases and productivity of biomass and secreted molecules in light-limited batch growth

(2018) Ryan L. Clark et al.   METABOLIC ENGINEERING

Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX

(2018) Danuta Kaczmarzyk et al.   METABOLIC ENGINEERING

Genome-wide fitness assessment during diurnal growth reveals an expanded role of the cyanobacterial circadian clock protein KaiA

(2018) David G. Welkie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Adjustments to Photosystem Stoichiometry and Electron Transfer Proteins Are Key to the Remarkably Fast Growth of the Cyanobacterium Synechococcus elongatus UTEX 2973

(2018) Justin Ungerer et al.   mBio

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

(2018) Tianmin Wang et al.   Nature Communications

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

(2018) Lun Cui et al.   Nature Communications

Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria

(2018) Kiyan Shabestary et al.   ACS Synthetic Biology

Mutant phenotypes for thousands of bacterial genes of unknown function

(2018) Morgan N. Price et al.   NATURE

Genome-scale fluxome of Synechococcus elongatus UTEX 2973 using transient 13C-labeling data

(2018) John I. Hendry et al.   PLANT PHYSIOLOGY

Comparative genomics reveals the molecular determinants of rapid growth of the cyanobacterium Synechococcus elongatus UTEX 2973

(2018) Justin Ungerer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Selective Enrichment of Slow-Growing Bacteria in a Metabolism-Wide CRISPRi Library with a TIMER Protein

(2018) Dominik Beuter et al.   ACS Synthetic Biology

Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins

(2018) Michael Jahn et al.   Cell Reports

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

(2018) François Rousset et al.   PLoS Genetics

Ribosome Profiling of Synechocystis Reveals Altered Ribosome Allocation at Carbon Starvation

(2018) Jan Karlsen et al.   mSystems

Microfluidics for cell factory and bioprocess development

(2018) Sara M Bjork et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic Flux Analysis of the Synechocystis sp. PCC 6803 ΔnrtABCD Mutant Reveals a Mechanism for Metabolic Adaptation to Nitrogen-Limited Conditions

(2017) Tsubasa Nakajima et al.   PLANT AND CELL PHYSIOLOGY

Acclimation to High CO 2 Requires the ω Subunit of the RNA Polymerase in Synechocystis

(2017) Juha Kurkela et al.   PLANT PHYSIOLOGY

When Do Two-Stage Processes Outperform One-Stage Processes?

(2017) Steffen Klamt et al.   Biotechnology Journal

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

Awakening of a Dormant Cyanobacterium from Nitrogen Chlorosis Reveals a Genetically Determined Program

(2016) Alexander Klotz et al.   CURRENT BIOLOGY

Cyanobacterial metabolic engineering for biofuel and chemical production

(2016) Neal J Oliver et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Can cyanobacteria serve as a model of plant photorespiration? – a comparative meta-analysis of metabolite profiles

(2016) Isabel Orf et al.   JOURNAL OF EXPERIMENTAL BOTANY

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

The Non-Coding RNA Ncr0700/PmgR1 is Required for Photomixotrophic Growth and the Regulation of Glycogen Accumulation in the CyanobacteriumSynechocystissp. PCC 6803

(2016) Alice J. de Porcellinis et al.   PLANT AND CELL PHYSIOLOGY

The Entner–Doudoroff pathway is an overlooked glycolytic route in cyanobacteria and plants

(2016) Xi Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Enhanced limonene production in cyanobacteria reveals photosynthesis limitations

(2016) Xin Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Photonfluxostat: A method for light-limited batch cultivation of cyanobacteria at different, yet constant, growth rates

(2016) Wei Du et al.   Algal Research-Biomass Biofuels and Bioproducts

Large-scale bioprocess competitiveness: the potential of dynamic metabolic control in two-stage fermentations

(2016) Jonathan M Burg et al.   Current Opinion in Chemical Engineering

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria

(2015) Petter Hammar et al.   Biotechnology for Biofuels

Circadian Rhythms in Cyanobacteria

(2015) Susan E. Cohen et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Identification of a Cyanobacterial RND-Type Efflux System Involved in Export of Free Fatty Acids

(2015) Akihiro Kato et al.   PLANT AND CELL PHYSIOLOGY

The essential gene set of a photosynthetic organism

(2015) Benjamin E. Rubin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

Phosphoketolase pathway contributes to carbon metabolism in cyanobacteria

(2015) Wei Xiong et al.   Nature Plants

Identification of OmpR-Family Response Regulators Interacting with Thioredoxin in the Cyanobacterium Synechocystis sp. PCC 6803

(2015) Taro Kadowaki et al.   PLoS One

Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803

(2014) S Angermayr et al.   Biotechnology for Biofuels

Comparative Analysis of the Primary Transcriptome of Synechocystis sp. PCC 6803

(2014) Matthias Kopf et al.   DNA RESEARCH

Cyanobacterial biofuels: new insights and strain design strategies revealed by computational modeling

(2014) Philipp Erdrich et al.   Microbial Cell Factories

The omega subunit of the RNA polymerase core directs transcription efficiency in cyanobacteria

(2014) Liisa Gunnelius et al.   NUCLEIC ACIDS RESEARCH

The ω Subunit of RNA Polymerase Is Essential for Thermal Acclimation of the Cyanobacterium Synechocystis Sp. PCC 6803

(2014) Liisa Gunnelius et al.   PLoS One

Electron transport and light-harvesting switches in cyanobacteria

(2014) Conrad W. Mullineaux   Frontiers in Plant Science

Wide-dynamic-range promoters engineered for cyanobacteria

(2013) Hsin-Ho Huang et al.   Journal of Biological Engineering

A novel counter-selection method for markerless genetic modification inSynechocystissp. PCC 6803

(2012) Yi Ern Cheah et al.   BIOTECHNOLOGY PROGRESS

Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803

(2012) Fernando Guerrero et al.   PLoS One

Genome-wide analysis of yeast stress survival and tolerance acquisition to analyze the central trade-off between growth rate and cellular robustness

(2011) Anna Zakrzewska et al.   MOLECULAR BIOLOGY OF THE CELL

Cyanobacterial NDH-1 complexes: Novel insights and remaining puzzles

(2010) Natalia Battchikova et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

High-throughput injection with microfluidics using picoinjectors

(2010) A. R. Abate et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

(2009) Jean-Christophe Baret et al.   LAB ON A CHIP