Regulated Expression of sgRNAs Tunes CRISPRi in E. coli

Enhanced solvent production by metabolic engineering of a twin-clostridial consortium

(2017) Zhiqiang Wen et al.   METABOLIC ENGINEERING

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit

(2017) Apoorv Gupta et al.   NATURE BIOTECHNOLOGY

Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

(2017) Weixin Tang et al.   Nature Communications

Rational design of inducible CRISPR guide RNAs for de novo assembly of transcriptional programs

(2017) Quentin R. V. Ferry et al.   Nature Communications

Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity

(2017) Eirik Adim Moreb et al.   ACS Synthetic Biology

Combining CRISPR and CRISPRi Systems for Metabolic Engineering of E. coli and 1,4-BDO Biosynthesis

(2017) Meng-Ying Wu et al.   ACS Synthetic Biology

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

CRISPR interference as a titratable, trans -acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp . strain PCC 7002

(2016) Gina C. Gordon et al.   METABOLIC ENGINEERING

CRISPR interference-guided balancing of a biosynthetic mevalonate pathway increases terpenoid production

(2016) Seong Keun Kim et al.   METABOLIC ENGINEERING

Directing cellular information flow via CRISPR signal conductors

(2016) Yuchen Liu et al.   NATURE METHODS

Kinetics of the CRISPR-Cas9 effector complex assembly and the role of 3′-terminal segment of guide RNA

(2016) Vladimir Mekler et al.   NUCLEIC ACIDS RESEARCH

Internal guide RNA interactions interfere with Cas9-mediated cleavage

(2016) Summer B. Thyme et al.   Nature Communications

Corynebacterium glutamicum Metabolic Engineering with CRISPR Interference (CRISPRi)

(2016) Sara Cleto et al.   ACS Synthetic Biology

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: Controllable P(3HB- co -4HB) biosynthesis

(2015) Li Lv et al.   METABOLIC ENGINEERING

Expanding the Biologist’s Toolkit with CRISPR-Cas9

(2015) Samuel H. Sternberg et al.   MOLECULAR CELL

Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation

(2015) Antonia A. Dominguez et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Automated physics-based design of synthetic riboswitches from diverse RNA aptamers

(2015) Amin Espah Borujeni et al.   NUCLEIC ACIDS RESEARCH

Enhancing flavonoid production by systematically tuning the central metabolic pathways based on a CRISPR interference system in Escherichia coli

(2015) Junjun Wu et al.   Scientific Reports

Multiple Gene Repression in Cyanobacteria Using CRISPRi

(2015) Lun Yao et al.   ACS Synthetic Biology

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Tunable protein degradation in bacteria

(2014) D Ewen Cameron et al.   NATURE BIOTECHNOLOGY

Designing RNA-Based Genetic Control Systems for Efficient Production from Engineered Metabolic Pathways

(2014) Jason T. Stevens et al.   ACS Synthetic Biology

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Knock-in/Knock-out (KIKO) vectors for rapid integration of large DNA sequences, including whole metabolic pathways, onto the Escherichia coli chromosome at well-characterised loci

(2013) Suriana Sabri et al.   Microbial Cell Factories

Engineering dynamic pathway regulation using stress-response promoters

(2013) Robert H Dahl et al.   NATURE BIOTECHNOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids

(2012) Fuzhong Zhang et al.   NATURE BIOTECHNOLOGY

Model-Driven Engineering of RNA Devices to Quantitatively Program Gene Expression

(2011) J. M. Carothers et al.   SCIENCE

BglBrick vectors and datasheets: A synthetic biology platform for gene expression

(2011) Taek Lee et al.   Journal of Biological Engineering

Engineering Static and Dynamic Control of Synthetic Pathways

(2010) William J. Holtz et al.   CELL

Chemical synthesis using synthetic biology

(2009) James M Carothers et al.   CURRENT OPINION IN BIOTECHNOLOGY