Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm Genetic Requirements in Enterococcus faecalis

A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus

(2020) Mathilde Guzzo et al.   mBio

The Enterococcus: a Model of Adaptability to Its Environment

(2019) Mónica García-Solache et al.   CLINICAL MICROBIOLOGY REVIEWS

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

(2019) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Conjugative delivery of CRISPR-Cas9 for the selective depletion of antibiotic-resistant enterococci

(2019) Marinelle Rodrigues et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

A Robust CRISPR Interference Gene Repression System in Pseudomonas

(2018) Sue Zanne Tan et al.   JOURNAL OF BACTERIOLOGY

Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

(2018) Antoine Vigouroux et al.   Molecular Systems Biology

An Attenuated CRISPR-Cas System in Enterococcus faecalis Permits DNA Acquisition

(2018) Karthik Hullahalli et al.   mBio

Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism

(2018) Damien Keogh et al.   mBio

Planktonic interference and biofilm alliance between aggregation substance and endocarditis and biofilm associated pili in Enterococcus faecalis

(2018) Irina Afonina et al.   JOURNAL OF BACTERIOLOGY

Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF

(2018) Jennifer L. Dale et al.   mSystems

Biofilm-associated infection by enterococci

(2018) Jun-Hong Ch’ng et al.   NATURE REVIEWS MICROBIOLOGY

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

(2018) François Rousset et al.   PLoS Genetics

The role of the CroR response regulator in resistance of Enterococcus faecalis to D-cycloserine is defined using an inducible receiver domain

(2017) Cécile Muller et al.   MOLECULAR MICROBIOLOGY

High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae

(2017) Xue Liu et al.   Molecular Systems Biology

Programmable transcriptional repression in mycobacteria using an orthogonal CRISPR interference platform

(2017) Jeremy M. Rock et al.   Nature Microbiology

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering

(2016) Addison V. Wright et al.   CELL

Functional Dissection of the CroRS Two-Component System Required for Resistance to Cell Wall Stressors in Enterococcus faecalis

(2016) Stephanie L. Kellogg et al.   JOURNAL OF BACTERIOLOGY

Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM

(2016) Alan S. L. Wong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CRISPR-Cas and Restriction-Modification Act Additively against Conjugative Antibiotic Resistance Plasmid Transfer in Enterococcus faecalis

(2016) Valerie J. Price et al.   mSphere

DNA library construction using Gibson Assembly®

(2015) Steven Thomas et al.   NATURE METHODS

Comparative Analysis of the Orphan CRISPR2 Locus in 242 Enterococcus faecalis Strains

(2015) Karthik Hullahalli et al.   PLoS One

The Enterococcus faecalis EbpA Pilus Protein: Attenuation of Expression, Biofilm Formation, and Adherence to Fibrinogen Start with the Rare Initiation Codon ATT

(2015) Maria Camila Montealegre et al.   mBio

Oxidative Stress Enhances Cephalosporin Resistance of Enterococcus faecalis through Activation of a Two-Component Signaling System

(2014) Dušanka Djorić et al.   ANTIMICROBIAL AGENTS AND CHEMOTHERAPY

DNA interrogation by the CRISPR RNA-guided endonuclease Cas9

(2014) Samuel H. Sternberg et al.   NATURE

Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases

(2014) Robert J Citorik et al.   NATURE BIOTECHNOLOGY

Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials

(2014) David Bikard et al.   NATURE BIOTECHNOLOGY

EbpA vaccine antibodies block binding of Enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice

(2014) A. L. Flores-Mireles et al.   Science Translational Medicine

Programmable Removal of Bacterial Strains by Use of Genome-Targeting CRISPR-Cas Systems

(2014) A. A. Gomaa et al.   mBio

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Pilin and Sortase Residues Critical for Endocarditis- and Biofilm-Associated Pilus Biogenesis in Enterococcus faecalis

(2013) H. V. Nielsen et al.   JOURNAL OF BACTERIOLOGY

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Contribution of Individual Ebp Pilus Subunits of Enterococcus faecalis OG1RF to Pilus Biogenesis, Biofilm Formation and Urinary Tract Infection

(2013) Jouko Sillanpää et al.   PLoS One

Development of a genomic site for gene integration and expression in Enterococcus faecalis

(2012) Sruti DebRoy et al.   JOURNAL OF MICROBIOLOGICAL METHODS

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

The Metal Ion-Dependent Adhesion Site Motif of the Enterococcus faecalis EbpA Pilin Mediates Pilus Function in Catheter-Associated Urinary Tract Infection

(2012) H. V. Nielsen et al.   mBio

Traffic Jam at the Bacterial Sec Translocase: Targeting the SecA Nanomotor by Small-Molecule Inhibitors

(2011) Kenneth Segers et al.   CHEMISTRY & BIOLOGY

Reciprocal Regulation of Cephalosporin Resistance in Enterococcus faecalis

(2011) C. J. Kristich et al.   mBio

Self versus non-self discrimination during CRISPR RNA-directed immunity

(2010) Luciano A. Marraffini et al.   NATURE

Mechanism for Sortase Localization and the Role of Sortase Localization in Efficient Pilus Assembly in Enterococcus faecalis

(2009) K. A. Kline et al.   JOURNAL OF BACTERIOLOGY

Development and Use of an Efficient System for Random mariner Transposon Mutagenesis To Identify Novel Genetic Determinants of Biofilm Formation in the Core Enterococcus faecalis Genome

(2008) C. J. Kristich et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

(2008) S. J. J. Brouns et al.   SCIENCE

Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF

(2008) Agathe Bourgogne et al.   GENOME BIOLOGY