Honing the Double-Edged Sword: Improving Human iPSC-Microglia Models

Human iPSC‐derived microglia: A growing toolset to study the brain's innate immune cells

(2020) Jonathan Hasselmann et al.   GLIA

Lipid-droplet-accumulating microglia represent a dysfunctional and proinflammatory state in the aging brain

(2020) Julia Marschallinger et al.   NATURE NEUROSCIENCE

A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation

(2020) Noah Goshi et al.   Journal of Neuroinflammation

Deciphering human macrophage development at single-cell resolution

(2020) Zhilei Bian et al.   NATURE

Microglia alterations in neurodegenerative diseases and their modeling with human induced pluripotent stem cell and other platforms

(2020) Angélica María Sabogal-Guáqueta et al.   PROGRESS IN NEUROBIOLOGY

Human iPSC-derived mature microglia retain their identity and functionally integrate in the chimeric mouse brain

(2020) Ranjie Xu et al.   Nature Communications

Lipid and Lipoprotein Metabolism in Microglia

(2020) Bailey A. Loving et al.   Frontiers in Physiology

A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture

(2020) Alexandra Grubman et al.   Stem Cell Reports

Large-Scale Production of Human iPSC-Derived Macrophages for Drug Screening

(2020) Simon Gutbier et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Alzheimer’s-associated PLCγ2 is a signaling node required for both TREM2 function and the inflammatory response in human microglia

(2020) Benjamin J. Andreone et al.   NATURE NEUROSCIENCE

Core Concept: How synaptic pruning shapes neural wiring during development and, possibly, in disease

(2020) Jill Sakai   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Investigating Microglia in Health and Disease: Challenges and Opportunities

(2020) Veronique E. Miron et al.   TRENDS IN IMMUNOLOGY

Emerging Developments in Human Induced Pluripotent Stem Cell-Derived Microglia: Implications for Modelling Psychiatric Disorders With a Neurodevelopmental Origin

(2020) Bjørn Hanger et al.   Frontiers in Psychiatry

Effects of extracellular matrix viscoelasticity on cellular behaviour

(2020) Ovijit Chaudhuri et al.   NATURE

Microglia and Astrocytes in Disease: Dynamic Duo or Partners in Crime?

(2020) Shane A. Liddelow et al.   TRENDS IN IMMUNOLOGY

Concise Review: Modeling Neurodegenerative Diseases with Human Pluripotent Stem Cell-Derived Microglia

(2019) Walther Haenseler et al.   STEM CELLS

Human Interleukin-34 facilitates microglia-like cell differentiation and persistent HIV-1 infection in humanized mice

(2019) Saumi Mathews et al.   Molecular Neurodegeneration

Development of a Chimeric Model to Study and Manipulate Human Microglia In Vivo

(2019) Jonathan Hasselmann et al.   NEURON

High glucose concentrations mask cellular phenotypes in a stem cell model of tuberous sclerosis complex

(2019) Paula Rocktäschel et al.   EPILEPSY & BEHAVIOR

Stem-cell-derived human microglia transplanted in mouse brain to study human disease

(2019) Renzo Mancuso et al.   NATURE NEUROSCIENCE

Three-dimensional (3D) brain microphysiological system for organophosphates and neurochemical agent toxicity screening

(2019) Lumei Liu et al.   PLoS One

Human iPSC-derived microglia assume a primary microglia-like state after transplantation into the neonatal mouse brain

(2019) Devon S. Svoboda et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A human co‐culture cell model incorporating microglia supports glioblastoma growth and migration, and confers resistance to cytotoxics

(2019) Diana M. Leite et al.   FASEB JOURNAL

Generating microglia from human pluripotent stem cells: novel in vitro models for the study of neurodegeneration

(2019) Anna M. Speicher et al.   Molecular Neurodegeneration

The influence of environment and origin on brain resident macrophages and implications for therapy

(2019) Mariko L. Bennett et al.   NATURE NEUROSCIENCE

Development of an In Vitro 3D Brain Tissue Model Mimicking In Vivo-Like Pro-inflammatory and Pro-oxidative Responses

(2018) Hyung Joon Cho et al.   ANNALS OF BIOMEDICAL ENGINEERING

A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer’s disease

(2018) Joseph Park et al.   NATURE NEUROSCIENCE

APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types

(2018) Yuan-Ta Lin et al.   NEURON

A transcriptomic atlas of aged human microglia

(2018) Marta Olah et al.   Nature Communications

Mechanical properties of the in vivo adolescent human brain

(2018) Grace McIlvain et al.   Developmental Cognitive Neuroscience

Functional Studies of Missense TREM2 Mutations in Human Stem Cell-Derived Microglia

(2018) Philip W. Brownjohn et al.   Stem Cell Reports

Corticosterone Preexposure Increases NF-κB Translocation and Sensitizes IL-1β Responses in BV2 Microglia-Like Cells

(2018) JiaJun Liu et al.   Frontiers in Immunology

Animal models of neurodegenerative diseases

(2018) Ted M. Dawson et al.   NATURE NEUROSCIENCE

Bidirectional Microglia–Neuron Communication in Health and Disease

(2018) Zsuzsanna Szepesi et al.   Frontiers in Cellular Neuroscience

Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis

(2018) Pablo Garcia-Reitboeck et al.   Cell Reports

The Kaleidoscope of Microglial Phenotypes

(2018) Marissa L. Dubbelaar et al.   Frontiers in Immunology

Human stem cell–derived monocytes and microglia-like cells reveal impaired amyloid plaque clearance upon heterozygous or homozygous loss of TREM2

(2018) Christel Claes et al.   Alzheimers & Dementia

Astrocytes and microglia: Models and tools

(2018) Kevin A. Guttenplan et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Development and validation of a simplified method to generate human microglia from pluripotent stem cells

(2018) Amanda McQuade et al.   Molecular Neurodegeneration

Microglia innately develop within cerebral organoids

(2018) Paul R. Ormel et al.   Nature Communications

Divergent immune responses to synthetic and biological scaffolds

(2018) Kaitlyn Sadtler et al.   BIOMATERIALS

Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function

(2017) Kazuyuki Takata et al.   IMMUNITY

Reactive Astrocytes: Production, Function, and Therapeutic Potential

(2017) Shane A. Liddelow et al.   IMMUNITY

Neurotoxic reactive astrocytes are induced by activated microglia

(2017) Shane A. Liddelow et al.   NATURE

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells

(2017) Hetal Pandya et al.   NATURE NEUROSCIENCE

Diverse Requirements for Microglial Survival, Specification, and Function Revealed by Defined-Medium Cultures

(2017) Christopher J. Bohlen et al.   NEURON

An environment-dependent transcriptional network specifies human microglia identity

(2017) David Gosselin et al.   SCIENCE

Coupled Proliferation and Apoptosis Maintain the Rapid Turnover of Microglia in the Adult Brain

(2017) Katharine Askew et al.   Cell Reports

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation

(2017) Kyle M. Koss et al.   Jove-Journal of Visualized Experiments

Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB -Independent Tissue-Resident Macrophages

(2017) Julian Buchrieser et al.   Stem Cell Reports

Directed Differentiation of Human Pluripotent Stem Cells to Microglia

(2017) Panagiotis Douvaras et al.   Stem Cell Reports

A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory Response

(2017) Walther Haenseler et al.   Stem Cell Reports

Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold

(2016) Ziyun Jiang et al.   ACS Applied Materials & Interfaces

Translation of Human-Induced Pluripotent Stem Cells

(2016) Nazish Sayed et al.   JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY

Efficient derivation of microglia-like cells from human pluripotent stem cells

(2016) Julien Muffat et al.   NATURE MEDICINE

Microglia development follows a stepwise program to regulate brain homeostasis

(2016) O. Matcovitch-Natan et al.   SCIENCE

Neuronal medium that supports basic synaptic functions and activity of human neurons in vitro

(2015) Cedric Bardy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Human pluripotent stem cell-derived neural constructs for predicting neural toxicity

(2015) Michael P. Schwartz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Hyaluronic Acid-Based 3D Culture Model for In Vitro Testing of Electrode Biocompatibility

(2014) Andrea F. Jeffery et al.   BIOMACROMOLECULES

Anti-inflammatory effects of three-dimensional graphene foams cultured with microglial cells

(2014) Qin Song et al.   BIOMATERIALS

A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia

(2014) Randy Tatt Haw et al.   Journal of Neuroinflammation

The role of the surface on microglia function: implications for central nervous system tissue engineering

(2014) L. R. Pires et al.   Journal of the Royal Society Interface

Plasticity of primary microglia on micropatterned geometries and spontaneous long-distance migration in microfluidic channels

(2013) Susanna Amadio et al.   BMC NEUROSCIENCE

Identification of a unique TGF-β–dependent molecular and functional signature in microglia

(2013) Oleg Butovsky et al.   NATURE NEUROSCIENCE

LPS-Induced Microglial Secretion of TNFα Increases Activity-Dependent Neuronal Apoptosis in the Neonatal Cerebral Cortex

(2012) Birgit Nimmervoll et al.   CEREBRAL CORTEX

TGF-β signaling through SMAD2/3 induces the quiescent microglial phenotype within the CNS environment

(2012) Shai Abutbul et al.   GLIA

Complex invasion pattern of the cerebral cortex bymicroglial cells during development of the mouse embryo

(2012) Nina Swinnen et al.   GLIA

Self-assembling peptide hydrogels promote microglial cells proliferation and NGF production

(2012) Laura Chronopoulou et al.   Soft Matter

Fabrication and optimization of alginate hydrogel constructs for use in 3D neural cell culture

(2011) J P Frampton et al.   Biomedical Materials

Synaptic Pruning by Microglia Is Necessary for Normal Brain Development

(2011) R. C. Paolicelli et al.   SCIENCE

Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages

(2010) F. Ginhoux et al.   SCIENCE

Marked differences in cholesterol synthesis between neurons and glial cells from postnatal rats

(2009) Katja Nieweg et al.   JOURNAL OF NEUROCHEMISTRY

Physiological glucose is critical for optimized neuronal viability and AMPK responsiveness in vitro

(2007) Amy M. Kleman et al.   JOURNAL OF NEUROSCIENCE METHODS