Genetically Encoded Biosensors Based on Fluorescent Proteins

Visualization of soluble tau oligomers in TauP301L-BiFC transgenic mice demonstrates the progression of tauopathy

(2020) Seulgi Shin et al.   PROGRESS IN NEUROBIOLOGY

Illuminating NAD+ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor

(2020) Yejun Zou et al.   DEVELOPMENTAL CELL

Monitoring alpha‐synuclein oligomerization and aggregation using bimolecular fluorescence complementation assays: what you see is not always what you get

(2020) Bryan Frey et al.   JOURNAL OF NEUROCHEMISTRY

Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator

(2020) Or A. Shemesh et al.   NEURON

Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

(2020) Nami Kitajima et al.   eLife

An expanded palette of dopamine sensors for multiplex imaging in vivo

(2020) Tommaso Patriarchi et al.   NATURE METHODS

Next-generation GRAB sensors for monitoring dopaminergic activity in vivo

(2020) Fangmiao Sun et al.   NATURE METHODS

Visualization of Autophagy Progression by a Red–Green–Blue Autophagy Sensor

(2020) Heejung Kim et al.   ACS Sensors

Intensiometric biosensors visualize the activity of multiple small GTPases in vivo

(2019) Jihoon Kim et al.   Nature Communications

pH-Lemon, a fluorescent protein-based pH reporter for acidic compartments

(2019) Sandra Burgstaller et al.   ACS Sensors

A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP

(2019) Mark A. Lobas et al.   Nature Communications

A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection of Norepinephrine

(2019) Jiesi Feng et al.   NEURON

FPbase: a community-editable fluorescent protein database

(2019) Talley J. Lambert   NATURE METHODS

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook

(2019) Matthew G. Romei et al.   Annual Review of Biophysics

Understanding the Fluorescence Change in Red Genetically Encoded Calcium Ion Indicators

(2019) Rosana S. Molina et al.   BIOPHYSICAL JOURNAL

High-performance calcium sensors for imaging activity in neuronal populations and microcompartments

(2019) Hod Dana et al.   NATURE METHODS

A genetically encoded fluorescent sensor for in vivo imaging of GABA

(2019) Jonathan S. Marvin et al.   NATURE METHODS

Biophysical basis underlying dynamic Lck activation visualized by ZapLck FRET biosensor

(2019) Rongxue Wan et al.   Science Advances

Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification

(2019) Alexander I. Kostyuk et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

α-Synuclein pathology in Parkinson’s disease and related α-synucleinopathies

(2019) Michael X. Henderson et al.   NEUROSCIENCE LETTERS

Pathogen‐induced pH changes regulate the growth‐defense balance in plants

(2019) Christopher Kesten et al.   EMBO JOURNAL

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice

(2019) Vincent Villette et al.   CELL

The Molecular Basis of G Protein–Coupled Receptor Activation

(2018) William I. Weis et al.   Annual Review of Biochemistry

Vinculin Force-Sensitive Dynamics at Focal Adhesions Enable Effective Directed Cell Migration

(2018) Katheryn E. Rothenberg et al.   BIOPHYSICAL JOURNAL

A Genetically Encoded Fluorescent Sensor Enables Rapid and Specific Detection of Dopamine in Flies, Fish, and Mice

(2018) Fangmiao Sun et al.   CELL

A genetically encoded fluorescent acetylcholine indicator for in vitro and in vivo studies

(2018) Miao Jing et al.   NATURE BIOTECHNOLOGY

Interregional synaptic maps among engram cells underlie memory formation

(2018) Jun-Hyeok Choi et al.   SCIENCE

Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

(2018) Tommaso Patriarchi et al.   SCIENCE

A high-avidity biosensor reveals plasma membrane PI(3,4)P2 is predominantly a class I PI3K signaling product

(2018) Brady D. Goulden et al.   JOURNAL OF CELL BIOLOGY

Stability, affinity, and chromatic variants of the glutamate sensor iGluSnFR

(2018) Jonathan S. Marvin et al.   NATURE METHODS

Improved methods for marking active neuron populations

(2018) Benjamien Moeyaert et al.   Nature Communications

Multiparameter imaging of calcium and abscisic acid and high-resolution quantitative calcium measurements using R-GECO1-mTurquoise in Arabidopsis

(2017) Rainer Waadt et al.   NEW PHYTOLOGIST

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

(2017) Erik A. Rodriguez et al.   TRENDS IN BIOCHEMICAL SCIENCES

Optical control of membrane tethering and interorganellar communication at nanoscales

(2017) Lian He et al.   Chemical Science

Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

(2017) Magalie Martineau et al.   Nature Communications

Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2

(2017) Marieke Mastop et al.   Scientific Reports

Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators

(2017) Simon Chamberland et al.   eLife

Red fluorescent protein-based cAMP indicator applicable to optogenetics and in vivo imaging

(2017) Kazuki Harada et al.   Scientific Reports

A Bi-fluorescence complementation system to detect associations between the Endoplasmic reticulum and mitochondria

(2017) Mark Harmon et al.   Scientific Reports

A Guide to Fluorescent Protein FRET Pairs

(2016) Bryce Bajar et al.   SENSORS

Protein Regulation in Signal Transduction

(2016) Michael J. Lee et al.   Cold Spring Harbor Perspectives in Biology

Versatile protein tagging in cells with split fluorescent protein

(2016) Daichi Kamiyama et al.   Nature Communications

RefSOFI for Mapping Nanoscale Organization of Protein-Protein Interactions in Living Cells

(2016) Fabian Hertel et al.   Cell Reports

Sensitive red protein calcium indicators for imaging neural activity

(2016) Hod Dana et al.   eLife

Natural Photoreceptors as a Source of Fluorescent Proteins, Biosensors, and Optogenetic Tools

(2015) Daria M. Shcherbakova et al.   Annual Review of Biochemistry

Quantitative analysis of autophagic flux by confocal pH-imaging of autophagic intermediates

(2015) Giuseppe Maulucci et al.   Autophagy

Detection and manipulation of phosphoinositides

(2015) Olof Idevall-Hagren et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Phosphoinositides in endocytosis

(2015) York Posor et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Understanding FRET as a Research Tool for Cellular Studies

(2015) Dilip Shrestha et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

(2015) Yidan Ding et al.   NATURE METHODS

Labeling of active neural circuits in vivo with designed calcium integrators

(2015) B. F. Fosque et al.   SCIENCE

Dynamic labelling of neural connections in multiple colours by trans-synaptic fluorescence complementation

(2015) Lindsey J. Macpherson et al.   Nature Communications

Comparison of genetically encoded calcium indicators for monitoring action potentials in mammalian brain by two-photon excitation fluorescence microscopy

(2015) Borbala Podor et al.   Neurophotonics

Chromophore chemistry of fluorescent proteins controlled by light

(2014) Daria M Shcherbakova et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis

(2014) Yi Shen et al.   JOURNAL OF CELL BIOLOGY

High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor

(2014) François St-Pierre et al.   NATURE NEUROSCIENCE

A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy

(2014) Isei Tanida et al.   PLoS One

Fluorescent proteins for live-cell imaging with super-resolution

(2013) Karin Nienhaus et al.   CHEMICAL SOCIETY REVIEWS

Conformational biosensors reveal GPCR signalling from endosomes

(2013) Roshanak Irannejad et al.   NATURE

Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation

(2013) Tamas Balla   PHYSIOLOGICAL REVIEWS

Bimolecular Fluorescence Complementation; Lighting-Up Tau-Tau Interaction in Living Cells

(2013) HyeJin Tak et al.   PLoS One

Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics

(2013) Jasper Akerboom et al.   Frontiers in Molecular Neuroscience

Fluorescence resonance energy transfer (FRET)-based biosensors: visualizing cellular dynamics and bioenergetics

(2012) Sohila Zadran et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Monitoring autophagic flux by an improved tandem fluorescent-tagged LC3 (mTagRFP-mWasabi-LC3) reveals that high-dose rapamycin impairs autophagic flux in cancer cells

(2012) Cuihong Zhou et al.   Autophagy

A Fluorogenic Red Fluorescent Protein Heterodimer

(2012) Spencer C. Alford et al.   CHEMISTRY & BIOLOGY

Live imaging of intra- and extracellular pH in plants using pHusion, a novel genetically encoded biosensor

(2012) Sisse Krag Gjetting et al.   JOURNAL OF EXPERIMENTAL BOTANY

An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging

(2012) Daria M. Shcherbakova et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Tandem fluorescent protein timers for in vivo analysis of protein dynamics

(2012) Anton Khmelinskii et al.   NATURE BIOTECHNOLOGY

pHTomato, a red, genetically encoded indicator that enables multiplex interrogation of synaptic activity

(2012) Yulong Li et al.   NATURE NEUROSCIENCE

Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%

(2012) Joachim Goedhart et al.   Nature Communications

Dimerization-Dependent Green and Yellow Fluorescent Proteins

(2012) Spencer C. Alford et al.   ACS Synthetic Biology

Development of Probes for Cellular Functions Using Fluorescent Proteins and Fluorescence Resonance Energy Transfer

(2011) Atsushi Miyawaki   Annual Review of Biochemistry

Stable expression of FRET biosensors: A new light in cancer research

(2011) Kazuhiro Aoki et al.   CANCER SCIENCE

mGRASP enables mapping mammalian synaptic connectivity with light microscopy

(2011) Jinhyun Kim et al.   NATURE METHODS

An Expanded Palette of Genetically Encoded Ca2+ Indicators

(2011) Y. Zhao et al.   SCIENCE

Detection of focal adhesion kinase activation at membrane microdomains by fluorescence resonance energy transfer

(2011) Jihye Seong et al.   Nature Communications

Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

(2010) Carsten Grashoff et al.   NATURE

Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues

(2010) Dmitriy M. Chudakov et al.   PHYSIOLOGICAL REVIEWS

A novel far-red bimolecular fluorescence complementation system that allows for efficient visualization of protein interactions under physiological conditions

(2009) Jun Chu et al.   BIOSENSORS & BIOELECTRONICS

Age-dependent Preferential Dense-Core Vesicle Exocytosis in Neuroendocrine Cells Revealed by Newly Developed Monomeric Fluorescent Timer Protein

(2009) Takashi Tsuboi et al.   MOLECULAR BIOLOGY OF THE CELL

Monomeric fluorescent timers that change color from blue to red report on cellular trafficking

(2009) Fedor V Subach et al.   Nature Chemical Biology

A bright and photostable photoconvertible fluorescent protein

(2009) Sean A McKinney et al.   NATURE METHODS

Bimolecular Fluorescence Complementation (BiFC) Analysis as a Probe of Protein Interactions in Living Cells

(2008) Tom K. Kerppola   Annual Review of Biophysics

Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells

(2008) Jin-Yu Fan et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Intracellular signaling dynamics during apoptosis execution in the presence or absence of X-linked-inhibitor-of-apoptosis-protein

(2008) Carla L. O'Connor et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

Real Time Analysis of Tumor Necrosis Factor-related Apoptosis-inducing Ligand/Cycloheximide-induced Caspase Activities during Apoptosis Initiation

(2008) Christian T. Hellwig et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Visualization of ternary complexes in living cells by using a BiFC-based FRET assay

(2008) Y John Shyu et al.   Nature Protocols

GFP Reconstitution Across Synaptic Partners (GRASP) Defines Cell Contacts and Synapses in Living Nervous Systems

(2008) Evan H. Feinberg et al.   NEURON

Visualization of AP-1 NF- B ternary complexes in living cells by using a BiFC-based FRET

(2008) Y. J. Shyu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA