Fluorescent proteins for live-cell imaging with super-resolution

Fast and Efficient Molecule Detection in Localization-Based Super-Resolution Microscopy by Parallel Adaptive Histogram Equalization

(2013) Yiming Li et al.   ACS Nano

A Bilirubin-Inducible Fluorescent Protein from Eel Muscle

(2013) Akiko Kumagai et al.   CELL

Small fluorescent nanoparticles at the nano–bio interface

(2013) Li Shang et al.   Materials Today

A New Set of Reversibly Photoswitchable Fluorescent Proteins for Use in Transgenic Plants

(2013) Martina Lummer et al.   Molecular Plant

A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum

(2013) Nathan C Shaner et al.   NATURE METHODS

Stimulated emission depletion-based raster image correlation spectroscopy reveals biomolecular dynamics in live cells

(2013) Per Niklas Hedde et al.   Nature Communications

Photophysics of Fluorescent Probes for Single-Molecule Biophysics and Super-Resolution Imaging

(2012) Taekjip Ha et al.   Annual Review of Physical Chemistry

Mechanistic Insights into Reversible Photoactivation in Proteins of the GFP Family

(2012) Susan Gayda et al.   BIOPHYSICAL JOURNAL

Modern fluorescent proteins: from chromophore formation to novel intracellular applications

(2012) Olesya Stepanenko et al.   BIOTECHNIQUES

Superresolution microscopy for microbiology

(2012) Carla Coltharp et al.   CELLULAR MICROBIOLOGY

STED Microscopy and its Applications: New Insights into Cellular Processes on the Nanoscale

(2012) Tobias Müller et al.   CHEMPHYSCHEM

Visualizing Cell Structure and Function with Point-Localization Superresolution Imaging

(2012) Prabuddha Sengupta et al.   DEVELOPMENTAL CELL

Dual Color Photoactivation Localization Microscopy of Cardiomyopathy-associated Desmin Mutants

(2012) Andreas Brodehl et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Insight into the Common Mechanism of the Chromophore Formation in the Red Fluorescent Proteins: The Elusive Blue Intermediate Revealed

(2012) Ksenia B. Bravaya et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

An Orange Fluorescent Protein with a Large Stokes Shift for Single-Excitation Multicolor FCCS and FRET Imaging

(2012) Daria M. Shcherbakova et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

Rational design of true monomeric and bright photoactivatable fluorescent proteins

(2012) Mingshu Zhang et al.   NATURE METHODS

mMaple: A Photoconvertible Fluorescent Protein for Use in Multiple Imaging Modalities

(2012) Ann L. McEvoy et al.   PLoS One

A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications

(2012) H. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

rsEGFP2 enables fast RESOLFT nanoscopy of living cells

(2012) Tim Grotjohann et al.   eLife

Review of Super-Resolution Fluorescence Microscopy for Biology

(2011) Bonnie O. Leung et al.   APPLIED SPECTROSCOPY

Superresolution Imaging of Multiple Fluorescent Proteins with Highly Overlapping Emission Spectra in Living Cells

(2011) Mudalige S. Gunewardene et al.   BIOPHYSICAL JOURNAL

Two-Color STED Microscopy of Living Synapses Using A Single Laser-Beam Pair

(2011) Jan Tønnesen et al.   BIOPHYSICAL JOURNAL

PALM and STORM: Unlocking live-cell super-resolution

(2011) Ricardo Henriques et al.   BIOPOLYMERS

Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications

(2011) Virgile Adam et al.   CHEMISTRY & BIOLOGY

From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family

(2011) Jörg Wiedenmann et al.   Journal of Biophotonics

The Nature of Transient Dark States in a Photoactivatable Fluorescent Protein

(2011) Arijit Roy et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Low-Temperature Chromophore Isomerization Reveals the Photoswitching Mechanism of the Fluorescent Protein Padron

(2011) Aline Regis Faro et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultra-small fluorescent metal nanoclusters: Synthesis and biological applications

(2011) Li Shang et al.   Nano Today

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

(2011) Tim Grotjohann et al.   NATURE

A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching

(2011) Tanja Brakemann et al.   NATURE BIOTECHNOLOGY

A photoswitchable orange-to-far-red fluorescent protein, PSmOrange

(2011) Oksana M Subach et al.   NATURE METHODS

Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging

(2011) Graham T Dempsey et al.   NATURE METHODS

Bleaching/blinking assisted localization microscopy for superresolution imaging using standard fluorescent molecules

(2011) D. T. Burnette et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Nonlinear structured-illumination microscopy with a photoswitchable protein reveals cellular structures at 50-nm resolution

(2011) E. H. Rego et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A New Wave of Cellular Imaging

(2010) Derek Toomre et al.   Annual Review of Cell and Developmental Biology

Far-Red Fluorescent Protein Excitable with Red Lasers for Flow Cytometry and Superresolution STED Nanoscopy

(2010) Kateryna S. Morozova et al.   BIOPHYSICAL JOURNAL

Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells

(2010) Bo Huang et al.   CELL

Structural Characterization of Acylimine-Containing Blue and Red Chromophores in mTagBFP and TagRFP Fluorescent Proteins

(2010) Oksana M. Subach et al.   CHEMISTRY & BIOLOGY

Red Fluorescent Protein with Reversibly Photoswitchable Absorbance for Photochromic FRET

(2010) Fedor V. Subach et al.   CHEMISTRY & BIOLOGY

The “Wiggling and Jiggling of Atoms” Leading to Excited-State Proton Transfer in Green Fluorescent Protein

(2010) G. Ulrich Nienhaus   CHEMPHYSCHEM

Molecular Basis of the Light-driven Switching of the Photochromic Fluorescent Protein Padron

(2010) Tanja Brakemann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A guide to super-resolution fluorescence microscopy

(2010) Lothar Schermelleh et al.   JOURNAL OF CELL BIOLOGY

Fluorescent proteins at a glance

(2010) G.-J. Kremers et al.   JOURNAL OF CELL SCIENCE

A Monomeric Photoconvertible Fluorescent Protein for Imaging of Dynamic Protein Localization

(2010) Hiofan Hoi et al.   JOURNAL OF MOLECULAR BIOLOGY

Photoactivatable Fluorescent Protein mEos2 Displays Repeated Photoactivation after a Long-Lived Dark State in the Red Photoconverted Form

(2010) P. Annibale et al.   Journal of Physical Chemistry Letters

Protonic Gating of Excited-State Twisting and Charge Localization in GFP Chromophores: A Mechanistic Hypothesis for Reversible Photoswitching

(2010) Seth Olsen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bright Monomeric Photoactivatable Red Fluorescent Protein for Two-Color Super-Resolution sptPALM of Live Cells

(2010) Fedor V. Subach et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Engineering ESPT Pathways Based on Structural Analysis of LSSmKate Red Fluorescent Proteins with Large Stokes Shift

(2010) Kiryl D. Piatkevich et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Fast, single-molecule localization that achieves theoretically minimum uncertainty

(2010) Carlas S Smith et al.   NATURE METHODS

Bright cyan fluorescent protein variants identified by fluorescence lifetime screening

(2010) Joachim Goedhart et al.   NATURE METHODS

A photoactivatable marker protein for pulse-chase imaging with superresolution

(2010) Jochen Fuchs et al.   NATURE METHODS

Ultra-fast, high-precision image analysis for localization-based super resolution microscopy

(2010) Tingwei Quan et al.   OPTICS EXPRESS

Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

(2010) Thomas Dertinger et al.   OPTICS EXPRESS

Higher resolution in localization microscopy by slower switching of a photochromic protein

(2010) Hideaki Mizuno et al.   PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES

Fluorescent Proteins and Their Applications in Imaging Living Cells and Tissues

(2010) Dmitriy M. Chudakov et al.   PHYSIOLOGICAL REVIEWS

Monomeric red fluorescent proteins with a large Stokes shift

(2010) K. D. Piatkevich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Group IV Nanoparticles: Synthesis, Properties, and Biological Applications

(2010) Jiyang Fan et al.   Small

Super-Resolution Fluorescence Microscopy

(2009) Bo Huang et al.   Annual Review of Biochemistry

Far-red fluorescent tags for protein imaging in living tissues

(2009) Dmitry Shcherbo et al.   BIOCHEMICAL JOURNAL

Structural Basis of Enhanced Photoconversion Yield in Green Fluorescent Protein-like Protein Dendra2

(2009) Virgile Adam et al.   BIOCHEMISTRY

Amphioxus encodes the largest known family of green fluorescent proteins, which have diversified into distinct functional classes

(2009) Erin K Bomati et al.   BMC EVOLUTIONARY BIOLOGY

The photochemistry of fluorescent proteins: implications for their biological applications

(2009) Harriet E. Seward et al.   CHEMICAL SOCIETY REVIEWS

GFP: from jellyfish to the Nobel prize and beyond

(2009) Marc Zimmer   CHEMICAL SOCIETY REVIEWS

The fluorescent protein palette: tools for cellular imaging

(2009) Richard N. Day et al.   CHEMICAL SOCIETY REVIEWS

Autofluorescent Proteins with Excitation in the Optical Window for Intravital Imaging in Mammals

(2009) Michael Z. Lin et al.   CHEMISTRY & BIOLOGY

The E1 Mechanism in Photo-Induced β-Elimination Reactions for Green-to-Red Conversion of Fluorescent Proteins

(2009) Hidekazu Tsutsui et al.   CHEMISTRY & BIOLOGY

Super-resolution optical microscopy: multiple choices

(2009) Bo Huang   CURRENT OPINION IN CHEMICAL BIOLOGY

Recent Advances of Fluorescent Technologies for Drug Discovery and Development

(2009) Chiranjib Chakraborty et al.   CURRENT PHARMACEUTICAL DESIGN

Fluorescent proteins for live cell imaging: Opportunities, limitations, and challenges

(2009) Jörg Wiedenmann et al.   IUBMB LIFE

Structural Basis for Phototoxicity of the Genetically Encoded Photosensitizer KillerRed

(2009) Sergei Pletnev et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

A Theoretical Study on the Nature of On- and Off-States of Reversibly Photoswitching Fluorescent Protein Dronpa: Absorption, Emission, Protonation, and Raman

(2009) Xin Li et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Photoconversion of the Fluorescent Protein EosFP: A Hybrid Potential Simulation Study Reveals Intersystem Crossings

(2009) Mickaël Lelimousin et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Structure and Mechanism of the Photoactivatable Green Fluorescent Protein

(2009) J. Nathan Henderson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Single Amino Acid Replacement Makes Aequorea victoria Fluorescent Proteins Reversibly Photoswitchable

(2009) Ranieri Bizzarri et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Age-dependent Preferential Dense-Core Vesicle Exocytosis in Neuroendocrine Cells Revealed by Newly Developed Monomeric Fluorescent Timer Protein

(2009) Takashi Tsuboi et al.   MOLECULAR BIOLOGY OF THE CELL

Mapping GFP structure evolution during proton transfer with femtosecond Raman spectroscopy

(2009) Chong Fang et al.   NATURE

Monomeric fluorescent timers that change color from blue to red report on cellular trafficking

(2009) Fedor V Subach et al.   Nature Chemical Biology

An ultramarine fluorescent protein with increased photostability and pH insensitivity

(2009) Wataru Tomosugi et al.   NATURE METHODS

Online image analysis software for photoactivation localization microscopy

(2009) Per Niklas Hedde et al.   NATURE METHODS

A bright and photostable photoconvertible fluorescent protein

(2009) Sean A McKinney et al.   NATURE METHODS

Photoactivatable mCherry for high-resolution two-color fluorescence microscopy

(2009) Fedor V Subach et al.   NATURE METHODS

Engineered fluorescent proteins: innovations and applications

(2009) Michael W Davidson et al.   NATURE METHODS

Subdiffraction resolution in continuous samples

(2009) Rainer Heintzmann et al.   Nature Photonics

mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures

(2009) Simone Kredel et al.   PLoS One

Photoactivation mechanism of PAmCherry based on crystal structures of the protein in the dark and fluorescent states

(2009) Fedor V. Subach et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI)

(2009) T. Dertinger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome

(2009) X. Shu et al.   SCIENCE

Photoactivatable fluorescent proteins for diffraction-limited and super-resolution imaging

(2009) Jennifer Lippincott-Schwartz et al.   TRENDS IN CELL BIOLOGY

Green fluorescent protein based pH indicators for in vivo use: a review

(2008) Ranieri Bizzarri et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

The Green Fluorescent Protein: A Key Tool to Study Chemical Processes in Living Cells

(2008) G. Ulrich Nienhaus   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Fluorescence Proteins, Live-Cell Imaging, and Mechanobiology: Seeing Is Believing

(2008) Yingxiao Wang et al.   Annual Review of Biomedical Engineering

Generation of Monomeric Reversibly Switchable Red Fluorescent Proteins for Far-Field Fluorescence Nanoscopy

(2008) Andre C. Stiel et al.   BIOPHYSICAL JOURNAL

Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

(2008) Hui-wang Ai et al.   BMC BIOLOGY

Visualizing Spatiotemporal Dynamics of Multicellular Cell-Cycle Progression

(2008) Asako Sakaue-Sawano et al.   CELL

GFP-like Proteins Stably Accumulate in Lysosomes

(2008) Hiroyuki Katayama et al.   CELL STRUCTURE AND FUNCTION

Ultrafast electronic and vibrational dynamics of stabilized A state mutants of the green fluorescent protein (GFP): Snipping the proton wire

(2008) Deborah Stoner-Ma et al.   CHEMICAL PHYSICS

Optimized and Far-Red-Emitting Variants of Fluorescent Protein eqFP611

(2008) Simone Kredel et al.   CHEMISTRY & BIOLOGY

Conversion of Red Fluorescent Protein into a Bright Blue Probe

(2008) Oksana M. Subach et al.   CHEMISTRY & BIOLOGY

Cis−TransPhotoisomerization of Fluorescent-Protein Chromophores

(2008) Valerio Voliani et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Trans−Cis Isomerization is Responsible for the Red-Shifted Fluorescence in Variants of the Red Fluorescent Protein eqFP611

(2008) Karin Nienhaus et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy

(2008) Martin Andresen et al.   NATURE BIOTECHNOLOGY

Improving membrane voltage measurements using FRET with new fluorescent proteins

(2008) Hidekazu Tsutsui et al.   NATURE METHODS

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

(2008) Hui-wang Ai et al.   NATURE METHODS

Improving the photostability of bright monomeric orange and red fluorescent proteins

(2008) Nathan C Shaner et al.   NATURE METHODS

Microscopy and its focal switch

(2008) Stefan W Hell   NATURE METHODS

Laboratory Evolution of Fast-Folding Green Fluorescent Protein Using Secretory Pathway Quality Control

(2008) Adam C. Fisher et al.   PLoS One

mKikGR, a Monomeric Photoswitchable Fluorescent Protein

(2008) Satoshi Habuchi et al.   PLoS One

Live-cell imaging of dendritic spines by STED microscopy

(2008) U. V. Nagerl et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural characterization of IrisFP, an optical highlighter undergoing multiple photo-induced transformations

(2008) V. Adam et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Stimulated emission depletion (STED) nanoscopy of a fluorescent protein-labeled organelle inside a living cell

(2008) B. Hein et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

NOBEL PRIZE IN CHEMISTRY: Three Scientists Bask in Prize's Fluorescent Glow

(2008) R. F. Service   SCIENCE