Understanding FRET as a Research Tool for Cellular Studies

Human T cells use CD1 and MR1 to recognize lipids and small molecules

(2014) Emilie Layre et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Maximum likelihood estimation of FRET efficiency and its implications for distortions in pixelwise calculation of FRET in microscopy

(2014) Peter Nagy et al.   CYTOMETRY PART A

Regulation of CD1a Surface Expression and Antigen Presentation by Invariant Chain and Lipid Rafts

(2014) I. Sloma et al.   JOURNAL OF IMMUNOLOGY

Nanobodies: Natural Single-Domain Antibodies

(2013) Serge Muyldermans   Annual Review of Biochemistry

The Fluid—Mosaic Model of Membrane Structure: Still relevant to understanding the structure, function and dynamics of biological membranes after more than 40years

(2013) Garth L. Nicolson   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

CD1d favors MHC neighborhood, GM1 ganglioside proximity and low detergent sensitive membrane regions on the surface of B lymphocytes

(2013) Dilip Shrestha et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Intensity Correlation-Based Calibration of FRET

(2013) László Bene et al.   BIOPHYSICAL JOURNAL

Inhibition of cytoplasmic streaming by cytochalasin D is superior to paraformaldehyde fixation for measuring FRET between fluorescent protein-tagged Golgi components

(2013) Christian Peter Poulsen et al.   CYTOMETRY PART A

TripleFRET measurements in flow cytometry

(2013) Ákos Fábián et al.   CYTOMETRY PART A

High throughput FRET analysis of protein-protein interactions by slide-based imaging laser scanning cytometry

(2013) Nikoletta Szalóki et al.   CYTOMETRY PART A

Technical Advance: Actin CytoFRET, a novel FRET flow cytometry method for detection of actin dynamics in resting and activated T cell

(2013) Frédéric Larbret et al.   JOURNAL OF LEUKOCYTE BIOLOGY

Fluorescent Proteins: Shine on, You Crazy Diamond

(2013) Peter Dedecker et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Estimating the distance separating fluorescent protein FRET pairs

(2013) Steven S. Vogel et al.   METHODS

Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt

(2013) Joshua A Broussard et al.   Nature Protocols

Molecular interactions of ErbB1 (EGFR) and integrin- 1 in astrocytoma frozen sections predict clinical outcome and correlate with Akt-mediated in vitro radioresistance

(2013) M. Petras et al.   NEURO-ONCOLOGY

The ErbB/HER family of protein-tyrosine kinases and cancer

(2013) Robert Roskoski   PHARMACOLOGICAL RESEARCH

Forming a Complex with MHC Class I Molecules Interferes with Mouse CD1d Functional Expression

(2013) Renukaradhya J. Gourapura et al.   PLoS One

Severe osmotic compression triggers a slowdown of intracellular signaling, which can be explained by molecular crowding

(2013) A. Miermont et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Paths to Förster’s resonance energy transfer (FRET) theory

(2013) B.R. Masters   European Physical Journal H

Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells

(2013) Sara M. Müller et al.   Frontiers in Plant Science

Single-molecule FRET of protein structure and dynamics - a primer

(2013) Benjamin Schuler   JOURNAL OF NANOBIOTECHNOLOGY

Comparative study of the three different fluorophore antibody conjugation strategies

(2012) Dilip Shrestha et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Quantitative Intensity-Based FRET Approaches—A Comparative Snapshot

(2012) André Zeug et al.   BIOPHYSICAL JOURNAL

Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors

(2012) Badri P. Maliwal et al.   JOURNAL OF BIOMEDICAL OPTICS

Dynamics within tetraspanin pairs affect MHC class II expression

(2012) T. van den Hoorn et al.   JOURNAL OF CELL SCIENCE

Synthetic antibodies: Concepts, potential and practical considerations

(2012) S. Miersch et al.   METHODS

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

(2012) Hellen C. Ishikawa-Ankerhold et al.   MOLECULES

Immunolabeling artifacts and the need for live-cell imaging

(2012) Ulrike Schnell et al.   NATURE METHODS

Improving FRET dynamic range with bright green and red fluorescent proteins

(2012) Amy J Lam et al.   NATURE METHODS

Segregation of molecules at cell division reveals native protein localization

(2012) Dirk Landgraf et al.   NATURE METHODS

Assessing the Tendency of Fluorescent Proteins to Oligomerize Under Physiologic Conditions

(2012) Lindsey M. Costantini et al.   TRAFFIC

Excessive interleukin-15 transpresentation endows NKG2D+CD4+ T cells with innate-like capacity to lyse vascular endothelium in granulomatosis with polyangiitis (Wegener's)

(2011) Mathilde de Menthon et al.   ARTHRITIS AND RHEUMATISM

Alternative affinity tools: more attractive than antibodies?

(2011) Vincent J. B. Ruigrok et al.   BIOCHEMICAL JOURNAL

Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics

(2011) Bin Wu et al.   CURRENT OPINION IN CELL BIOLOGY

Crohn's disease alters MHC-rafts in CD4+ T-cells

(2011) László Damjanovich et al.   CYTOMETRY PART A

Quantification of protein interaction in living cells by two-photon spectral imaging with fluorescent protein fluorescence resonance energy transfer pair devoid of acceptor bleed-through

(2011) Jiho Kim et al.   CYTOMETRY PART A

T-cell synapse formation depends on antigen recognition but not CD3 interaction: Studies with TCR:ζ, a candidate transgene for TCR gene therapy

(2011) János Roszik et al.   EUROPEAN JOURNAL OF IMMUNOLOGY

SpRET: Highly Sensitive and Reliable Spectral Measurement of Absolute FRET Efficiency

(2011) Shiri Levy et al.   MICROSCOPY AND MICROANALYSIS

Imaging proteins inside cells with fluorescent tags

(2011) Georgeta Crivat et al.   TRENDS IN BIOTECHNOLOGY

Coclustering of ErbB1 and ErbB2 Revealed by FRET-Sensitized Acceptor Bleaching

(2010) Ágnes Szabó et al.   BIOPHYSICAL JOURNAL

Fluorescence Lifetime Measurements and Biological Imaging

(2010) Mikhail Y. Berezin et al.   CHEMICAL REVIEWS

Red Fluorescent Protein with Reversibly Photoswitchable Absorbance for Photochromic FRET

(2010) Fedor V. Subach et al.   CHEMISTRY & BIOLOGY

HomoFRET Fluorescence Anisotropy Imaging as a Tool to Study Molecular Self-Assembly in Live Cells

(2010) Fiona T. S. Chan et al.   CHEMPHYSCHEM

Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications

(2010) J. Löfblom et al.   FEBS LETTERS

Signalling complexes and clusters: functional advantages and methodological hurdles

(2010) M. Cebecauer et al.   JOURNAL OF CELL SCIENCE

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Evaluation of intensity-based ratiometric FRET in image cytometry-Approaches and a software solution

(2009) János Roszik et al.   CYTOMETRY PART A

Anomalous Surplus Energy Transfer Observed with Multiple FRET Acceptors

(2009) Srinagesh V. Koushik et al.   PLoS One

Quantitative Characterization of the Large-Scale Association of ErbB1 and ErbB2 by Flow Cytometric Homo-FRET Measurements

(2008) Ágnes Szabó et al.   BIOPHYSICAL JOURNAL

AccPbFRET: An ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images

(2008) János Roszik et al.   BMC BIOINFORMATICS

An Engineered Protein Tag for Multiprotein Labeling in Living Cells

(2008) Arnaud Gautier et al.   CHEMISTRY & BIOLOGY

High throughput FRET screening of the plasma membrane based on TIRFM

(2008) Christian Paar et al.   CYTOMETRY PART A

A biophysical approach to IL-2 and IL-15 receptor function: Localization, conformation and interactions

(2008) Andrea Bodnár et al.   IMMUNOLOGY LETTERS

All EGF(ErbB) receptors have preformed homo- and heterodimeric structures in living cells

(2008) R.-H. Tao et al.   JOURNAL OF CELL SCIENCE

Novel lambda FRET spectral confocal microscopy imaging method

(2008) Diego Megías et al.   MICROSCOPY RESEARCH AND TECHNIQUE

Conformation of the c-Fos/c-Jun Complex In Vivo: A Combined FRET, FCCS, and MD-Modeling Study

(2007) György Vámosi et al.   BIOPHYSICAL JOURNAL

Two-sided fluorescence resonance energy transfer for assessing molecular interactions of up to three distinct species in confocal microscopy

(2007) Zsolt Fazekas et al.   CYTOMETRY PART A