Astrocytes and microglia in neurodegenerative diseases: Lessons from human in vitro models

Human iPSC‐derived microglia: A growing toolset to study the brain's innate immune cells

(2020) Jonathan Hasselmann et al.   GLIA

Early Fate Defines Microglia and Non-parenchymal Brain Macrophage Development

(2020) Sebastian G. Utz et al.   CELL

Microglia alterations in neurodegenerative diseases and their modeling with human induced pluripotent stem cell and other platforms

(2020) Angélica María Sabogal-Guáqueta et al.   PROGRESS IN NEUROBIOLOGY

Human iPSC-derived mature microglia retain their identity and functionally integrate in the chimeric mouse brain

(2020) Ranjie Xu et al.   Nature Communications

LRRK2 Is Recruited to Phagosomes and Co-recruits RAB8 and RAB10 in Human Pluripotent Stem Cell-Derived Macrophages

(2020) Heyne Lee et al.   Stem Cell Reports

Regionally encoded functional heterogeneity of astrocytes in health and disease: A perspective

(2020) Benjamin E. Clarke et al.   GLIA

CD49f Is a Novel Marker of Functional and Reactive Human iPSC-Derived Astrocytes

(2020) Lilianne Barbar et al.   NEURON

Human stem cell-derived astrocytes exhibit region-specific heterogeneity in their secretory profiles

(2020) Benjamin E Clarke et al.   BRAIN

Human Induced Pluripotent Stem Cell-Derived Neural Cells from Alzheimer’s Disease Patients Exhibited Different Susceptibility to Oxidative Stress

(2020) Lin Zhang et al.   STEM CELLS AND DEVELOPMENT

Gene expression and functional deficits underlie TREM2-knockout microglia responses in human models of Alzheimer’s disease

(2020) Amanda McQuade et al.   Nature Communications

Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells

(2020) Vasiliki Panagiotakopoulou et al.   Nature Communications

The microglial component of amyotrophic lateral sclerosis

(2020) Benjamin E Clarke et al.   BRAIN

Generation of pure monocultures of human microglia-like cells from induced pluripotent stem cells

(2020) Poulomi Banerjee et al.   Stem Cell Research

TREM2 Alzheimer’s variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages

(2020) Hazel Hall-Roberts et al.   Alzheimers Research & Therapy

Patient-Specific iPSC-Derived Astrocytes Contribute to Non-Cell-Autonomous Neurodegeneration in Parkinson's Disease

(2019) Angelique di Domenico et al.   Stem Cell Reports

Micro-RNAs secreted through astrocyte-derived extracellular vesicles cause neuronal network degeneration in C9orf72 ALS

(2019) André Varcianna et al.   EBioMedicine

Concise Review: Modeling Neurodegenerative Diseases with Human Pluripotent Stem Cell-Derived Microglia

(2019) Walther Haenseler et al.   STEM CELLS

NFIA is a gliogenic switch enabling rapid derivation of functional human astrocytes from pluripotent stem cells

(2019) Jason Tchieu et al.   NATURE BIOTECHNOLOGY

Astrocyte adenosine deaminase loss increases motor neuron toxicity in amyotrophic lateral sclerosis

(2019) Scott P Allen et al.   BRAIN

Regionally specified human pluripotent stem cell-derived astrocytes exhibit different molecular signatures and functional properties

(2019) Robert A. Bradley et al.   DEVELOPMENT

Delineating Astrocytic Cytokine Responses in a Human Stem Cell Model of Neural Trauma

(2019) Eric Peter Thelin et al.   JOURNAL OF NEUROTRAUMA

RNA sequencing reveals MMP2 and TGFB1 downregulation in LRRK2 G2019S Parkinson's iPSC-derived astrocytes

(2019) Heather D.E. Booth et al.   NEUROBIOLOGY OF DISEASE

Huntington’s Disease Patient-Derived Astrocytes Display Electrophysiological Impairments and Reduced Neuronal Support

(2019) Veronica J. Garcia et al.   Frontiers in Neuroscience

Stem-cell-derived human microglia transplanted in mouse brain to study human disease

(2019) Renzo Mancuso et al.   NATURE NEUROSCIENCE

Human iPSC-derived microglia assume a primary microglia-like state after transplantation into the neonatal mouse brain

(2019) Devon S. Svoboda et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Role of Human‐Induced Pluripotent Stem Cell‐Derived Spinal Cord Astrocytes in the Functional Maturation of Motor Neurons in a Multielectrode Array System

(2019) Arens Taga et al.   Stem Cells Translational Medicine

PSEN1ΔE9, APPswe, and APOE4 Confer Disparate Phenotypes in Human iPSC-Derived Microglia

(2019) Henna Konttinen et al.   Stem Cell Reports

A locked immunometabolic switch underlies TREM2 R47H loss of function in human iPSC‐derived microglia

(2019) Thomas M. Piers et al.   FASEB JOURNAL

Mutant C9orf72 human iPSC‐derived astrocytes cause non‐cell autonomous motor neuron pathophysiology

(2019) Chen Zhao et al.   GLIA

Convergence between Microglia and Peripheral Macrophages Phenotype during Development and Neuroinflammation

(2019) Francesca Grassivaro et al.   JOURNAL OF NEUROSCIENCE

A characterization of Gaucher iPS-derived astrocytes: Potential implications for Parkinson's disease

(2019) Elma Aflaki et al.   NEUROBIOLOGY OF DISEASE

Human iPSC-derived astrocytes from ALS patients with mutated C9ORF72 show increased oxidative stress and neurotoxicity

(2019) Anastasya Birger et al.   EBioMedicine

LRRK2 is a negative regulator of Mycobacterium tuberculosis phagosome maturation in macrophages

(2018) Anetta Härtlova et al.   EMBO JOURNAL

A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer’s disease

(2018) Joseph Park et al.   NATURE NEUROSCIENCE

Modelling microglial function with induced pluripotent stem cells: an update

(2018) Jennifer M. Pocock et al.   NATURE REVIEWS NEUROSCIENCE

Kir4.1-Dependent Astrocyte-Fast Motor Neuron Interactions Are Required for Peak Strength

(2018) Kevin W. Kelley et al.   NEURON

A Combination of Ontogeny and CNS Environment Establishes Microglial Identity

(2018) F. Chris Bennett et al.   NEURON

APOE4 Causes Widespread Molecular and Cellular Alterations Associated with Alzheimer’s Disease Phenotypes in Human iPSC-Derived Brain Cell Types

(2018) Yuan-Ta Lin et al.   NEURON

Interleukin 4 modulates microglia homeostasis and attenuates the early slowly progressive phase of amyotrophic lateral sclerosis

(2018) Chiara Rossi et al.   Cell Death & Disease

Functional Studies of Missense TREM2 Mutations in Human Stem Cell-Derived Microglia

(2018) Philip W. Brownjohn et al.   Stem Cell Reports

Microglia in neurodegeneration

(2018) Suzanne Hickman et al.   NATURE NEUROSCIENCE

Microglial signatures and their role in health and disease

(2018) Oleg Butovsky et al.   NATURE REVIEWS NEUROSCIENCE

Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis

(2018) Pablo Garcia-Reitboeck et al.   Cell Reports

Fast Generation of Functional Subtype Astrocytes from Human Pluripotent Stem Cells

(2018) Xiang Li et al.   Stem Cell Reports

Harnessing cellular aging in human stem cell models of amyotrophic lateral sclerosis

(2018) Oliver J. Ziff et al.   AGING CELL

Human stem cell–derived monocytes and microglia-like cells reveal impaired amyloid plaque clearance upon heterozygous or homozygous loss of TREM2

(2018) Christel Claes et al.   Alzheimers & Dementia

Pathological Changes in Alzheimer’s Disease Analyzed Using Induced Pluripotent Stem Cell-Derived Human Microglia-Like Cells

(2018) Mei Xu et al.   JOURNAL OF ALZHEIMERS DISEASE

Development and validation of a simplified method to generate human microglia from pluripotent stem cells

(2018) Amanda McQuade et al.   Molecular Neurodegeneration

Modelling Sporadic Alzheimer’s Disease Using Induced Pluripotent Stem Cells

(2018) Helen A. Rowland et al.   NEUROCHEMICAL RESEARCH

Human Induced Pluripotent Stem Cell-Derived Astrocytes Are Differentially Activated by Multiple Sclerosis-Associated Cytokines

(2018) Sylvain Perriot et al.   Stem Cell Reports

APOE ε4/ε4 diminishes neurotrophic function of human iPSC-derived astrocytes

(2017) Jing Zhao et al.   HUMAN MOLECULAR GENETICS

Reactive Astrocytes: Production, Function, and Therapeutic Potential

(2017) Shane A. Liddelow et al.   IMMUNITY

Neurotoxic reactive astrocytes are induced by activated microglia

(2017) Shane A. Liddelow et al.   NATURE

Microglia emerge as central players in brain disease

(2017) Michael W Salter et al.   NATURE MEDICINE

Transcriptomic analysis of purified human cortical microglia reveals age-associated changes

(2017) Thais F Galatro et al.   NATURE NEUROSCIENCE

Differentiation of human and murine induced pluripotent stem cells to microglia-like cells

(2017) Hetal Pandya et al.   NATURE NEUROSCIENCE

A new fate mapping system reveals context-dependent random or clonal expansion of microglia

(2017) Tuan Leng Tay et al.   NATURE NEUROSCIENCE

In vitro α-synuclein neurotoxicity and spreading among neurons and astrocytes using Lewy body extracts from Parkinson disease brains

(2017) Fabio Cavaliere et al.   NEUROBIOLOGY OF DISEASE

iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases

(2017) Edsel M. Abud et al.   NEURON

Human Astrocyte Maturation Captured in 3D Cerebral Cortical Spheroids Derived from Pluripotent Stem Cells

(2017) Steven A. Sloan et al.   NEURON

An environment-dependent transcriptional network specifies human microglia identity

(2017) David Gosselin et al.   SCIENCE

A human microglia-like cellular model for assessing the effects of neurodegenerative disease gene variants

(2017) Katie J. Ryan et al.   Science Translational Medicine

The Role of Astrocyte Dysfunction in Parkinson’s Disease Pathogenesis

(2017) Heather D.E. Booth et al.   TRENDS IN NEUROSCIENCES

A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models

(2017) Giulia E. Tyzack et al.   Nature Communications

Progressive Motor Neuron Pathology and the Role of Astrocytes in a Human Stem Cell Model of VCP-Related ALS

(2017) Claire E. Hall et al.   Cell Reports

Major Shifts in Glial Regional Identity Are a Transcriptional Hallmark of Human Brain Aging

(2017) Lilach Soreq et al.   Cell Reports

Microglial Activation in the Pathogenesis of Huntington’s Disease

(2017) Hui-Ming Yang et al.   Frontiers in Aging Neuroscience

Amyotrophic lateral sclerosis patient iPSC-derived astrocytes impair autophagy via non-cell autonomous mechanisms

(2017) Martin Madill et al.   Molecular Brain

PSEN1 Mutant iPSC-Derived Model Reveals Severe Astrocyte Pathology in Alzheimer's Disease

(2017) Minna Oksanen et al.   Stem Cell Reports

Systematic Three-Dimensional Coculture Rapidly Recapitulates Interactions between Human Neurons and Astrocytes

(2017) Robert Krencik et al.   Stem Cell Reports

Sporadic ALS Astrocytes Induce Neuronal Degeneration In Vivo

(2017) Kun Qian et al.   Stem Cell Reports

Human Induced Pluripotent Stem Cell-Derived Macrophages Share Ontogeny with MYB -Independent Tissue-Resident Macrophages

(2017) Julian Buchrieser et al.   Stem Cell Reports

Directed Differentiation of Human Pluripotent Stem Cells to Microglia

(2017) Panagiotis Douvaras et al.   Stem Cell Reports

A Highly Efficient Human Pluripotent Stem Cell Microglia Model Displays a Neuronal-Co-culture-Specific Expression Profile and Inflammatory Response

(2017) Walther Haenseler et al.   Stem Cell Reports

Amyotrophic lateral sclerosis

(2017) Orla Hardiman et al.   Nature Reviews Disease Primers

Excess α-synuclein compromises phagocytosis in iPSC-derived macrophages

(2017) Walther Haenseler et al.   Scientific Reports

An updated review of Parkinson's disease genetics and clinicopathological correlations

(2016) M. Ferreira et al.   ACTA NEUROLOGICA SCANDINAVICA

The amyloid cascade hypothesis: are we poised for success or failure?

(2016) Eric Karran et al.   JOURNAL OF NEUROCHEMISTRY

Dysfunctional Calcium and Glutamate Signaling in Striatal Astrocytes from Huntington's Disease Model Mice

(2016) R. Jiang et al.   JOURNAL OF NEUROSCIENCE

Astrocyte scar formation aids central nervous system axon regeneration

(2016) Mark A. Anderson et al.   NATURE

Efficient derivation of microglia-like cells from human pluripotent stem cells

(2016) Julien Muffat et al.   NATURE MEDICINE

A polarizing question: do M1 and M2 microglia exist?

(2016) Richard M Ransohoff   NATURE NEUROSCIENCE

Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse

(2016) Ye Zhang et al.   NEURON

Human glia can both induce and rescue aspects of disease phenotype in Huntington disease

(2016) Abdellatif Benraiss et al.   Nature Communications

Precision Modulation of Neurodegenerative Disease-Related Gene Expression in Human iPSC-Derived Neurons

(2016) Sabrina Mahalia Heman-Ackah et al.   Scientific Reports

Directly Reprogrammed Human Neurons Retain Aging-Associated Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic Defects

(2015) Jerome Mertens et al.   Cell Stem Cell

Human glial progenitor engraftment and gene expression is independent of the ALS environment

(2015) Amanda M. Haidet-Phillips et al.   EXPERIMENTAL NEUROLOGY

Genetic Cell Ablation Reveals Clusters of Local Self-Renewing Microglia in the Mammalian Central Nervous System

(2015) Julia Bruttger et al.   IMMUNITY

Establishment and characterization of a new conditionally immortalized human astrocyte cell line

(2015) Tomomi Furihata et al.   JOURNAL OF NEUROCHEMISTRY

Differentiation of reactive-like astrocytes cultured on nanofibrillar and comparative culture surfaces

(2015) Volkan Müjdat Tiryaki et al.   Nanomedicine

Prion-like transmission of neuronal huntingtin aggregates to phagocytic glia in the Drosophila brain

(2015) Margaret M. P. Pearce et al.   Nature Communications

Controlling the Regional Identity of hPSC-Derived Neurons to Uncover Neuronal Subtype Specificity of Neurological Disease Phenotypes

(2015) Kent Imaizumi et al.   Stem Cell Reports

Huntington disease

(2015) Gillian P. Bates et al.   Nature Reviews Disease Primers

Mechanisms of astrocyte development and their contributions to neurodevelopmental disorders

(2014) Steven A Sloan et al.   CURRENT OPINION IN NEUROBIOLOGY

Glial cells in amyotrophic lateral sclerosis

(2014) T. Philips et al.   EXPERIMENTAL NEUROLOGY

LPS and IL-1 differentially activate mouse and human astrocytes: Role of CD14

(2014) Leonid Tarassishin et al.   GLIA

Astrocytic TGF-  Signaling Limits Inflammation and Reduces Neuronal Damage during Central Nervous System Toxoplasma Infection

(2014) E. Cekanaviciute et al.   JOURNAL OF IMMUNOLOGY

Age-Dependent, Non-Cell-Autonomous Deposition of Amyloid from Synthesis of  -Amyloid by Cells Other Than Excitatory Neurons

(2014) K. Veeraraghavalu et al.   JOURNAL OF NEUROSCIENCE

Tissue-resident macrophages originate from yolk-sac-derived erythro-myeloid progenitors

(2014) Elisa Gomez Perdiguero et al.   NATURE

Astrocyte Kir4.1 ion channel deficits contribute to neuronal dysfunction in Huntington's disease model mice

(2014) Xiaoping Tong et al.   NATURE NEUROSCIENCE

Gaucher iPSC-Derived Macrophages Produce Elevated Levels of Inflammatory Mediators and Serve as a New Platform for Therapeutic Development

(2014) Leelamma M. Panicker et al.   STEM CELLS

Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease

(2014) Masahiro Ohgidani et al.   Scientific Reports

Forebrain Engraftment by Human Glial Progenitor Cells Enhances Synaptic Plasticity and Learning in Adult Mice

(2013) Xiaoning Han et al.   Cell Stem Cell

Modeling Alzheimer’s Disease with iPSCs Reveals Stress Phenotypes Associated with Intracellular Aβ and Differential Drug Responsiveness

(2013) Takayuki Kondo et al.   Cell Stem Cell

Innate Immunity in Alzheimer’s Disease: A Complex Affair

(2013) Marie-Victoire Guillot-Sestier et al.   CNS & Neurological Disorders-Drug Targets

Bioactive 3D cell culture system minimizes cellular stress and maintains thein vivo-like morphological complexity of astroglial cells

(2013) Till B. Puschmann et al.   GLIA

A critical role of astrocyte-mediated nuclear factor-κB-dependent inflammation in Huntington's disease

(2013) Han-Yun Hsiao et al.   HUMAN MOLECULAR GENETICS

Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent pathways

(2013) Katrin Kierdorf et al.   NATURE NEUROSCIENCE

Identification of a unique TGF-β–dependent molecular and functional signature in microglia

(2013) Oleg Butovsky et al.   NATURE NEUROSCIENCE

Apolipoprotein E and Alzheimer disease: risk, mechanisms and therapy

(2013) Chia-Chen Liu et al.   Nature Reviews Neurology

Physiological Roles of Aquaporin-4 in Brain

(2013) Erlend A. Nagelhus et al.   PHYSIOLOGICAL REVIEWS

Direct conversion of patient fibroblasts demonstrates non-cell autonomous toxicity of astrocytes to motor neurons in familial and sporadic ALS

(2013) K. Meyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Astrocyte pathology and the absence of non-cell autonomy in an induced pluripotent stem cell model of TDP-43 proteinopathy

(2013) A. Serio et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Multicenter Study of Glucocerebrosidase Mutations in Dementia With Lewy Bodies

(2013) Michael A. Nalls et al.   JAMA Neurology

Regulated temporal-spatial astrocyte precursor cell proliferation involves BRAF signalling in mammalian spinal cord

(2012) A.-C. Tien et al.   DEVELOPMENT

Disease-specific phenotypes in dopamine neurons from human iPS-based models of genetic and sporadic Parkinson's disease

(2012) Adriana Sánchez-Danés et al.   EMBO Molecular Medicine

Investigating the utility of human embryonic stem cell-derived neurons to model ageing and neurodegenerative disease using whole-genome gene expression and splicing analysis

(2012) Rickie Patani et al.   JOURNAL OF NEUROCHEMISTRY

LRRK2 Inhibition Attenuates Microglial Inflammatory Responses

(2012) M. S. Moehle et al.   JOURNAL OF NEUROSCIENCE

A novel in vitro human microglia model: Characterization of human monocyte-derived microglia

(2012) Samar Etemad et al.   JOURNAL OF NEUROSCIENCE METHODS

Local generation of glia is a major astrocyte source in postnatal cortex

(2012) Woo-Ping Ge et al.   NATURE

Sox9 and NFIA Coordinate a Transcriptional Regulatory Cascade during the Initiation of Gliogenesis

(2012) Peng Kang et al.   NEURON

Induced pluripotent stem cell model recapitulates pathologic hallmarks of Gaucher disease

(2012) L. M. Panicker et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Lineage of Myeloid Cells Independent of Myb and Hematopoietic Stem Cells

(2012) C. Schulz et al.   SCIENCE

Regional Astrocyte Allocation Regulates CNS Synaptogenesis and Repair

(2012) H.-H. Tsai et al.   SCIENCE

Induced Pluripotent Stem Cell Models of Progranulin-Deficient Frontotemporal Dementia Uncover Specific Reversible Neuronal Defects

(2012) Sandra Almeida et al.   Cell Reports

Astrocytes generated from patient induced pluripotent stem cells recapitulate features of Huntington’s disease patient cells

(2012) Tarja A Juopperi et al.   Molecular Brain

Kynurenine 3-Monooxygenase Inhibition in Blood Ameliorates Neurodegeneration

(2011) Daniel Zwilling et al.   CELL

Human embryonic stem cell derived astrocytes mediate non-cell-autonomous neuroprotection through endogenous and drug-induced mechanisms

(2011) K Gupta et al.   CELL DEATH AND DIFFERENTIATION

LRRK2 Mutant iPSC-Derived DA Neurons Demonstrate Increased Susceptibility to Oxidative Stress

(2011) Ha Nam Nguyen et al.   Cell Stem Cell

Specification of transplantable astroglial subtypes from human pluripotent stem cells

(2011) Robert Krencik et al.   NATURE BIOTECHNOLOGY

Development of a Method for the Purification and Culture of Rodent Astrocytes

(2011) Lynette C. Foo et al.   NEURON

Mitochondria: The Next (Neurode)Generation

(2011) Eric A. Schon et al.   NEURON

Direct Transfer of α-Synuclein from Neuron to Astroglia Causes Inflammatory Responses in Synucleinopathies

(2010) He-Jin Lee et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages

(2010) F. Ginhoux et al.   SCIENCE

Astrocytic expression of Parkinson's disease-related A53T α-synuclein causes neurodegeneration in mice

(2010) Xing-Long Gu et al.   Molecular Brain

Astrocytes: biology and pathology

(2009) Michael V. Sofroniew et al.   ACTA NEUROPATHOLOGICA

Macrophage colony stimulating factor (M-CSF) exacerbates ALS disease in a mouse model through altered responses of microglia expressing mutant superoxide dismutase

(2009) Genevieve Gowing et al.   EXPERIMENTAL NEUROLOGY

A versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis

(2009) Emma East et al.   Journal of Tissue Engineering and Regenerative Medicine

Multicenter Analysis of Glucocerebrosidase Mutations in Parkinson's Disease

(2009) E. Sidransky et al.   NEW ENGLAND JOURNAL OF MEDICINE

Expression of mutant huntingtin in mouse brain astrocytes causes age-dependent neurological symptoms

(2009) Jennifer Bradford et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Microglial Dysfunction and Defective  -Amyloid Clearance Pathways in Aging Alzheimer's Disease Mice

(2008) S. E. Hickman et al.   JOURNAL OF NEUROSCIENCE

Astrocytes as determinants of disease progression in inherited amyotrophic lateral sclerosis

(2008) Koji Yamanaka et al.   NATURE NEUROSCIENCE