Linkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler

Proteolysis-Targeting Chimeras as Therapeutics and Tools for Biological Discovery

(2020) George M. Burslem et al.   CELL

Harnessing the Power of Proteolysis for Targeted Protein Inactivation

(2020) Rati Verma et al.   MOLECULAR CELL

NEDD8 nucleates a multivalent cullin–RING–UBE2D ubiquitin ligation assembly

(2020) Kheewoong Baek et al.   NATURE

Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation

(2020) Emma Branigan et al.   Nature Communications

Diverse fate of ubiquitin chain moieties: The proximal is degraded with the target, and the distal protects the proximal from removal and recycles

(2019) Hao Sun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A conserved asparagine in a ubiquitin‐conjugating enzyme positions the substrate for nucleophilic attack

(2019) Walker M. Jones et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Insights into ubiquitin chain architecture using Ub-clipping

(2019) Kirby N. Swatek et al.   NATURE

Structural and Functional Analysis of Ubiquitin-based Inhibitors That Target the Backsides of E2 Enzymes

(2019) Pankaj Garg et al.   JOURNAL OF MOLECULAR BIOLOGY

Robust cullin-RING ligase function is established by a multiplicity of poly-ubiquitylation pathways

(2019) Spencer Hill et al.   eLife

Activity-based E3 ligase profiling uncovers an E3 ligase with esterification activity

(2018) Kuan-Chuan Pao et al.   NATURE

UBE2G1 governs the destruction of cereblon neomorphic substrates

(2018) Gang Lu et al.   eLife

Defining the human C2H2 zinc finger degrome targeted by thalidomide analogs through CRBN

(2018) Quinlan L. Sievers et al.   SCIENCE

Molecular Basis for K63-Linked Ubiquitination Processes in Double-Strand DNA Break Repair: A Focus on Kinetics and Dynamics

(2017) Brian L. Lee et al.   JOURNAL OF MOLECULAR BIOLOGY

An Interaction Landscape of Ubiquitin Signaling

(2017) Xiaofei Zhang et al.   MOLECULAR CELL

The Ubiquitin Code in the Ubiquitin-Proteasome System and Autophagy

(2017) Yong Tae Kwon et al.   TRENDS IN BIOCHEMICAL SCIENCES

Lysine Possesses the Optimal Chain Length for Histone Lysine Methyltransferase Catalysis

(2017) Abbas H. K. Al Temimi et al.   Scientific Reports

Two Distinct Types of E3 Ligases Work in Unison to Regulate Substrate Ubiquitylation

(2016) Daniel C. Scott et al.   CELL

Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C

(2016) Nicholas G. Brown et al.   CELL

Synthetic Uncleavable Ubiquitinated Proteins Dissect Proteasome Deubiquitination and Degradation, and Highlight Distinctive Fate of Tetraubiquitin

(2016) Sumeet K. Singh et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanism of Lysine 48 Selectivity during Polyubiquitin Chain Formation by the Ube2R1/2 Ubiquitin-Conjugating Enzyme

(2016) Spencer Hill et al.   MOLECULAR AND CELLULAR BIOLOGY

The increasing complexity of the ubiquitin code

(2016) Richard Yau et al.   NATURE CELL BIOLOGY

Structural insights into the catalysis and regulation of E3 ubiquitin ligases

(2016) Lori Buetow et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes

(2016) Florian Weissmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains

(2015) Emma Branigan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains

(2015) Emma Branigan et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of a RING E3 Trapped in Action Reveals Ligation Mechanism for the Ubiquitin-like Protein NEDD8

(2014) Daniel C. Scott et al.   CELL

Differential Ubiquitin Binding by the Acidic Loops of Ube2g1 and Ube2r1 Enzymes Distinguishes Their Lys-48-ubiquitylation Activities

(2014) Yun-Seok Choi et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Stochastic Gate Dynamics Regulate the Catalytic Activity of Ubiquitination Enzymes

(2014) Manoj K. Rout et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Mechanism of Polyubiquitination by Human Anaphase-Promoting Complex: RING Repurposing for Ubiquitin Chain Assembly

(2014) Nicholas G. Brown et al.   MOLECULAR CELL

Intrinsic disorder drives N-terminal ubiquitination by Ube2w

(2014) Vinayak Vittal et al.   Nature Chemical Biology

Minimal, encapsulated proteomic-sample processing applied to copy-number estimation in eukaryotic cells

(2014) Nils A Kulak et al.   NATURE METHODS

Lysine-targeting specificity in ubiquitin and ubiquitin-like modification pathways

(2014) Francesca Mattiroli et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Multimodal Mechanism of Action for the Cdc34 Acidic Loop

(2013) Amy Ziemba et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural basis for ligase-specific conjugation of linear ubiquitin chains by HOIP

(2013) Benjamin Stieglitz et al.   NATURE

A conserved asparagine has a structural role in ubiquitin-conjugating enzymes

(2013) Christopher E Berndsen et al.   Nature Chemical Biology

Mechanisms and function of substrate recruitment by F-box proteins

(2013) Jeffrey R. Skaar et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming

(2013) Elena Maspero et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Lenalidomide Causes Selective Degradation of IKZF1 and IKZF3 in Multiple Myeloma Cells

(2013) J. Kronke et al.   SCIENCE

The Myeloma Drug Lenalidomide Promotes the Cereblon-Dependent Destruction of Ikaros Proteins

(2013) G. Lu et al.   SCIENCE

Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3

(2013) Hari B Kamadurai et al.   eLife

The Ubiquitin Code

(2012) David Komander et al.   Annual Review of Biochemistry

The E3 ligase HOIP specifies linear ubiquitin chain assembly through its RING-IBR-RING domain and the unique LDD extension

(2012) Judith J Smit et al.   EMBO JOURNAL

The Mechanism of Linkage-Specific Ubiquitin Chain Elongation by a Single-Subunit E2

(2011) Katherine E. Wickliffe et al.   CELL

UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids

(2011) Dawn M. Wenzel et al.   NATURE

Mechanism of ubiquitylation by dimeric RING ligase RNF4

(2011) Anna Plechanovová et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Semisynthetic Src SH2 Domains Demonstrate Altered Phosphopeptide Specificity Induced by Incorporation of Unnatural Lysine Derivatives

(2010) Satpal Virdee et al.   CHEMISTRY & BIOLOGY

Lys11-linked ubiquitin chains adopt compact conformations and are preferentially hydrolyzed by the deubiquitinase Cezanne

(2010) Anja Bremm et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Polyubiquitination by HECT E3s and the Determinants of Chain Type Specificity

(2009) H. C. Kim et al.   MOLECULAR AND CELLULAR BIOLOGY

Ubiquitin-binding domains — from structures to functions

(2009) Ivan Dikic et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural Insights into NEDD8 Activation of Cullin-RING Ligases: Conformational Control of Conjugation

(2008) David M. Duda et al.   CELL