Control strategies to manage trade-offs during microbial production

Heterogeneity coordinates bacterial multi-gene expression in single cells

(2020) Yichao Han et al.   PLoS Computational Biology

Metabolite Sequestration Enables Rapid Recovery from Fatty Acid Depletion in Escherichia coli

(2020) Christopher J. Hartline et al.   mBio

Bacterial metabolic heterogeneity: origins and applications in engineering and infectious disease

(2020) Trent D Evans et al.   CURRENT OPINION IN BIOTECHNOLOGY

Coupling metabolic addiction with negative autoregulation to improve strain stability and pathway yield

(2020) Yongkun Lv et al.   METABOLIC ENGINEERING

Biosensor-assisted high performing cell selection using an E. coli toxin/antitoxin system

(2019) Xiaonan Wang et al.   BIOCHEMICAL ENGINEERING JOURNAL

Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

(2019) Xiaozhou Luo et al.   NATURE

Biosynthesis, regulation, and engineering of microbially produced branched biofuels

(2019) Wenqin Bai et al.   Biotechnology for Biofuels

Coupling feedback genetic circuits with growth phenotype for dynamic population control and intelligent bioproduction

(2019) Yongkun Lv et al.   METABOLIC ENGINEERING

A universal biomolecular integral feedback controller for robust perfect adaptation

(2019) Stephanie K. Aoki et al.   NATURE

Overcoming genetic heterogeneity in industrial fermentations

(2019) Peter Rugbjerg et al.   NATURE BIOTECHNOLOGY

Displaced by Deceivers: Prevention of Biosensor Cross-Talk Is Pivotal for Successful Biosensor-Based High-Throughput Screening Campaigns

(2019) Lion Konstantin Flachbart et al.   ACS Synthetic Biology

De novo phenol bioproduction from glucose using biosensor‐assisted microbial coculture engineering

(2019) Xiaoyun Guo et al.   BIOTECHNOLOGY AND BIOENGINEERING

Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production

(2019) Jian Wang et al.   METABOLIC ENGINEERING

Programmable biomolecular switches for rewiring flux in Escherichia coli

(2019) Cong Gao et al.   Nature Communications

Seeded Chain-Growth Polymerization of Proteins in Living Bacterial Cells

(2019) Christopher Hyde Bowen et al.   ACS Synthetic Biology

Steps towards ‘drop-in’ biofuels: focusing on metabolic pathways

(2018) Wen Jiang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Production of chemicals using dynamic control of metabolic fluxes

(2018) Peng Xu   CURRENT OPINION IN BIOTECHNOLOGY

Dynamic metabolic control: towards precision engineering of metabolism

(2018) Di Liu et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Optogenetic regulation of engineered cellular metabolism for microbial chemical production

(2018) Evan M. Zhao et al.   NATURE

Burden-driven feedback control of gene expression

(2018) Francesca Ceroni et al.   NATURE METHODS

Metabolic division of labor in microbial systems

(2018) Ryan Tsoi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Layered dynamic regulation for improving metabolic pathway productivity inEscherichia coli

(2018) Stephanie J. Doong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synthetic addiction extends the productive life time of engineeredEscherichia colipopulations

(2018) Peter Rugbjerg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Dynamic allocation of orthogonal ribosomes facilitates uncoupling of co-expressed genes

(2018) Alexander P. S. Darlington et al.   Nature Communications

An Orthogonal and pH-Tunable Sensor-Selector for Muconic Acid Biosynthesis in Yeast

(2018) Tim Snoek et al.   ACS Synthetic Biology

Metabolic Feedback Circuits Provide Rapid Control of Metabolite Dynamics

(2018) Di Liu et al.   ACS Synthetic Biology

Recombinant Spidroins Fully Replicate Primary Mechanical Properties of Natural Spider Silk

(2018) Christopher H. Bowen et al.   BIOMACROMOLECULES

High production of 4-hydroxyisoleucine in Corynebacterium glutamicum by multistep metabolic engineering

(2018) Chenglin Zhang et al.   METABOLIC ENGINEERING

Metabolic engineering in the host Yarrowia lipolytica

(2018) Ahmad M. Abdel-Mawgoud et al.   METABOLIC ENGINEERING

Controlling and exploiting cell-to-cell variation in metabolic engineering

(2018) Tiebin Wang et al.   CURRENT OPINION IN BIOTECHNOLOGY

Recent advancements in fungal-derived fuel and chemical production and commercialization

(2018) Holly Ekas et al.   CURRENT OPINION IN BIOTECHNOLOGY

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit

(2017) Apoorv Gupta et al.   NATURE BIOTECHNOLOGY

Growth-coupled overproduction is feasible for almost all metabolites in five major production organisms

(2017) Axel von Kamp et al.   Nature Communications

Fundamental Design Principles for Transcription-Factor-Based Metabolite Biosensors

(2017) Ahmad A. Mannan et al.   ACS Synthetic Biology

Engineering Microbial Metabolite Dynamics and Heterogeneity

(2017) Alexander C. Schmitz et al.   Biotechnology Journal

Model-based metabolic engineering enables high yield itaconic acid production by Escherichia coli

(2016) Björn-Johannes Harder et al.   METABOLIC ENGINEERING

Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis

(2016) Yi Xiao et al.   Nature Chemical Biology

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis inEscherichia coli

(2016) Bradley Walters Biggs et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Metabolic Burden: Cornerstones in Synthetic Biology and Metabolic Engineering Applications

(2016) Gang Wu et al.   TRENDS IN BIOTECHNOLOGY

Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth

(2016) Andreas Milias-Argeitis et al.   Nature Communications

Antithetic Integral Feedback Ensures Robust Perfect Adaptation in Noisy Biomolecular Networks

(2016) Corentin Briat et al.   Cell Systems

Isocost Lines Describe the Cellular Economy of Genetic Circuits

(2015) Andras Gyorgy et al.   BIOPHYSICAL JOURNAL

Engineering metabolism through dynamic control

(2015) Naveen Venayak et al.   CURRENT OPINION IN BIOTECHNOLOGY

Quorum-sensing linked RNA interference for dynamic metabolic pathway control in Saccharomyces cerevisiae

(2015) T.C. Williams et al.   METABOLIC ENGINEERING

Self-induced metabolic state switching by a tunable cell density sensor for microbial isopropanol production

(2015) Yuki Soma et al.   METABOLIC ENGINEERING

On the feasibility of growth-coupled product synthesis in microbial strains

(2015) Steffen Klamt et al.   METABOLIC ENGINEERING

Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae

(2015) Wenping Xie et al.   METABOLIC ENGINEERING

Applications and advances of metabolite biosensors for metabolic engineering

(2015) Di Liu et al.   METABOLIC ENGINEERING

Overflow metabolism in Escherichia coli results from efficient proteome allocation

(2015) Markus Basan et al.   NATURE

Quantifying cellular capacity identifies gene expression designs with reduced burden

(2015) Francesca Ceroni et al.   NATURE METHODS

Complete biosynthesis of opioids in yeast

(2015) S. Galanie et al.   SCIENCE

Improving fatty acids production by engineering dynamic pathway regulation and metabolic control

(2014) P. Xu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Evolution-guided optimization of biosynthetic pathways

(2014) Srivatsan Raman et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Engineering dynamic pathway regulation using stress-response promoters

(2013) Robert H Dahl et al.   NATURE BIOTECHNOLOGY

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

Negative Feedback Regulation of Fatty Acid Production Based on a Malonyl-CoA Sensor–Actuator

(2013) Di Liu et al.   ACS Synthetic Biology

Dynamic control of gene expression in Saccharomyces cerevisiae engineered for the production of plant sesquitepene α-santalene in a fed-batch mode

(2012) Gionata Scalcinati et al.   METABOLIC ENGINEERING

Microbial engineering for the production of advanced biofuels

(2012) Pamela P. Peralta-Yahya et al.   NATURE

Metabolic engineering of Escherichia coli for direct production of 1,4-butanediol

(2011) Harry Yim et al.   Nature Chemical Biology

Biosensors and their applications in microbial metabolic engineering

(2011) Fuzhong Zhang et al.   TRENDS IN MICROBIOLOGY

Interdependence of Cell Growth and Gene Expression: Origins and Consequences

(2010) M. Scott et al.   SCIENCE

Model-driven evaluation of the production potential for growth-coupled products of Escherichia coli

(2009) Adam M. Feist et al.   METABOLIC ENGINEERING