Overcoming genetic heterogeneity in industrial fermentations

Highly sensitive detection of mutations in CHO cell recombinant DNA using multi-parallel single molecule real-time DNA sequencing

(2018) Joseph F. Cartwright et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of chemicals using dynamic control of metabolic fluxes

(2018) Peng Xu   CURRENT OPINION IN BIOTECHNOLOGY

Burden-driven feedback control of gene expression

(2018) Francesca Ceroni et al.   NATURE METHODS

Enhancing the accuracy of next-generation sequencing for detecting rare and subclonal mutations

(2018) Jesse J. Salk et al.   NATURE REVIEWS GENETICS

Synthetic addiction extends the productive life time of engineeredEscherichia colipopulations

(2018) Peter Rugbjerg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Diverse genetic error modes constrain large-scale bio-based production

(2018) Peter Rugbjerg et al.   Nature Communications

An Expanded Heterologous GAL Promoter Collection for Diauxie-Inducible Expression in Saccharomyces cerevisiae

(2018) Bingyin Peng et al.   ACS Synthetic Biology

Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis

(2018) Tao Yu et al.   CELL

Directed evolution of Escherichia coli with lower-than-natural plasmid mutation rates

(2018) Daniel E Deatherage et al.   NUCLEIC ACIDS RESEARCH

Expression vector-derived heterogeneity in a therapeutic IgG4 monoclonal antibody

(2018) Douglas S. Rehder et al.   mAbs

Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution

(2017) Hemanshu Mundhada et al.   METABOLIC ENGINEERING

Gene Architectures that Minimize Cost of Gene Expression

(2017) Idan Frumkin et al.   MOLECULAR CELL

Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit

(2017) Apoorv Gupta et al.   NATURE BIOTECHNOLOGY

Design of a synthetic yeast genome

(2017) Sarah M. Richardson et al.   SCIENCE

Mammalian Systems Biotechnology Reveals Global Cellular Adaptations in a Recombinant CHO Cell Line

(2017) Faraaz Noor Khan Yusufi et al.   Cell Systems

Engineering Cellular Metabolism

(2016) Jens Nielsen et al.   CELL

Development of a commercial scale process for production of 1,4-butanediol from sugar

(2016) Anthony Burgard et al.   CURRENT OPINION IN BIOTECHNOLOGY

Developing fermentative terpenoid production for commercial usage

(2016) Michael D Leavell et al.   CURRENT OPINION IN BIOTECHNOLOGY

Modular co-culture engineering, a new approach for metabolic engineering

(2016) Haoran Zhang et al.   METABOLIC ENGINEERING

Evolved plasmid-host interactions reduce plasmid interference cost

(2016) Hirokazu Yano et al.   MOLECULAR MICROBIOLOGY

Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis

(2016) Yi Xiao et al.   Nature Chemical Biology

Functional mining of transporters using synthetic selections

(2016) Hans J Genee et al.   Nature Chemical Biology

Genetic circuit design automation

(2016) A. A. K. Nielsen et al.   SCIENCE

Metabolic Burden: Cornerstones in Synthetic Biology and Metabolic Engineering Applications

(2016) Gang Wu et al.   TRENDS IN BIOTECHNOLOGY

The Cost of Protein Production

(2016) Moshe Kafri et al.   Cell Reports

Sequencing the CHO DXB11 genome reveals regional variations in genomic stability and haploidy

(2015) Christian Kaas et al.   BMC GENOMICS

Engineering metabolism through dynamic control

(2015) Naveen Venayak et al.   CURRENT OPINION IN BIOTECHNOLOGY

Systems strategies for developing industrial microbial strains

(2015) Sang Yup Lee et al.   NATURE BIOTECHNOLOGY

Engineering an allosteric transcription factor to respond to new ligands

(2015) Noah D Taylor et al.   NATURE METHODS

Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction

(2015) Soon-Kyeong Kwon et al.   Scientific Reports

De-convoluting the Genetic Adaptations of E. coli C41(DE3) in Real Time Reveals How Alleviating Protein Production Stress Improves Yields

(2015) Susan Schlegel et al.   Cell Reports

Detecting rare structural variation in evolving microbial populations from new sequence junctions using breseq

(2015) Daniel E. Deatherage et al.   Frontiers in Genetics

Transcription-Associated Mutagenesis

(2014) Sue Jinks-Robertson et al.   Annual Review of Genetics

Amino acid misincorporation in recombinant biopharmaceutical products

(2014) Robert P Harris et al.   CURRENT OPINION IN BIOTECHNOLOGY

Polycistronic expression of a β-carotene biosynthetic pathway in Saccharomyces cerevisiae coupled to β-ionone production

(2014) Jules Beekwilder et al.   JOURNAL OF BIOTECHNOLOGY

Use of pantothenate as a metabolic switch increases the genetic stability of farnesene producing Saccharomyces cerevisiae

(2014) Celeste M. Sandoval et al.   METABOLIC ENGINEERING

Engineering reduced evolutionary potential for synthetic biology

(2014) Brian A. Renda et al.   Molecular BioSystems

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Mutational landscape of yeast mutator strains

(2014) Alexandre Serero et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multivariate PAT solutions for biopharmaceutical cultivation: current progress and limitations

(2014) Sarah M. Mercier et al.   TRENDS IN BIOTECHNOLOGY

G/U and Certain Wobble Position Mismatches as Possible Main Causes of Amino Acid Misincorporations

(2013) Zhongqi Zhang et al.   BIOCHEMISTRY

Long-term adaptation ofSaccharomyces cerevisiaeto the burden of recombinant insulin production

(2013) Ali Kazemi Seresht et al.   BIOTECHNOLOGY AND BIOENGINEERING

From the first drop to the first truckload: commercialization of microbial processes for renewable chemicals

(2013) Stephen Van Dien   CURRENT OPINION IN BIOTECHNOLOGY

Overcoming the metabolic burden of protein secretion in Schizosaccharomyces pombe – A quantitative approach using 13C-based metabolic flux analysis

(2013) Tobias Klein et al.   METABOLIC ENGINEERING

Engineering dynamic pathway regulation using stress-response promoters

(2013) Robert H Dahl et al.   NATURE BIOTECHNOLOGY

Metabolite damage and its repair or pre-emption

(2013) Carole L Linster et al.   Nature Chemical Biology

Determination of Chinese hamster ovary cell line stability and recombinant antibody expression during long-term culture

(2012) Laura A Bailey et al.   BIOTECHNOLOGY AND BIOENGINEERING

Characterization of plasmid burden and copy number inSaccharomyces cerevisiaefor optimization of metabolic engineering applications

(2012) Ashty S. Karim et al.   FEMS YEAST RESEARCH

Metabolic responses to recombinant bioprocesses in Escherichia coli

(2012) Sónia Carneiro et al.   JOURNAL OF BIOTECHNOLOGY

Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform

(2012) Michael Dalgaard Mikkelsen et al.   METABOLIC ENGINEERING

Visualizing evolution in real time to determine the molecular mechanisms of n-butanol tolerance in Escherichia coli

(2012) Luis H. Reyes et al.   METABOLIC ENGINEERING

Low-mutation-rate, reduced-genome Escherichia coli: An improved host for faithful maintenance of engineered genetic constructs

(2012) Balint Csorgo et al.   Microbial Cell Factories

Low Level Sequence Variant Analysis of Recombinant Proteins: An Optimized Approach

(2012) Anne Zeck et al.   PLoS One

Identification and treatment of heme depletion attributed to overexpression of a lineage of evolved P450 monooxygenases

(2012) J. K. Michener et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rate and molecular spectrum of spontaneous mutations in the bacterium Escherichia coli as determined by whole-genome sequencing

(2012) H. Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CHO cells in biotechnology for production of recombinant proteins: current state and further potential

(2011) Jee Yon Kim et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Early prediction of instability of chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins

(2011) Haimanti Dorai et al.   BIOTECHNOLOGY AND BIOENGINEERING

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

(2011) Xun Xu et al.   NATURE BIOTECHNOLOGY

Sequence-specific error profile of Illumina sequencers

(2011) Kensuke Nakamura et al.   NUCLEIC ACIDS RESEARCH

Metabolic engineering of Saccharomyces cerevisiae for the biotechnological production of succinic acid

(2010) Andreas M. Raab et al.   METABOLIC ENGINEERING

Misfolded proteins impose a dosage-dependent fitness cost and trigger a cytosolic unfolded protein response in yeast

(2010) K. A. Geiler-Samerotte et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber

(2010) X.-X. Xia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plasmid addiction systems: perspectives and applications in biotechnology

(2010) Jens Kroll et al.   Microbial Biotechnology

De Novo Biosynthesis of Vanillin in Fission Yeast (Schizosaccharomyces pombe) and Baker's Yeast (Saccharomyces cerevisiae)

(2009) E. H. Hansen et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Application of Functional Genomics to Pathway Optimization for Increased Isoprenoid Production

(2008) L. Kizer et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Induction of multiple pleiotropic drug resistance genes in yeast engineered to produce an increased level of anti-malarial drug precursor, artemisinic acid

(2008) Dae-Kyun Ro et al.   BMC BIOTECHNOLOGY

The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse

(2008) T. Durfee et al.   JOURNAL OF BACTERIOLOGY

Potential for metabolic engineering of resveratrol biosynthesis

(2008) Coralie Halls et al.   TRENDS IN BIOTECHNOLOGY