A guide to assessing cellular senescence in vitro and in vivo

A proteomic atlas of senescence-associated secretomes for aging biomarker development

(2020) Nathan Basisty et al.   PLOS BIOLOGY

Survey of senescent cell markers with age in human tissues

(2020) M. Laura Idda et al.   Aging-US

Functional heterogeneity in senescence

(2020) Kristina Kirschner et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

The Bright and Dark Side of Extracellular Vesicles in the Senescence-Associated Secretory Phenotype

(2020) Ryan Wallis et al.   MECHANISMS OF AGEING AND DEVELOPMENT

Defined p16High Senescent Cell Types Are Indispensable for Mouse Healthspan

(2020) Laurent Grosse et al.   Cell Metabolism

Senolytic CAR T cells reverse senescence-associated pathologies

(2020) Corina Amor et al.   NATURE

Senescence, Necrosis, and Apoptosis Govern Circulating Cell-free DNA Release Kinetics

(2020) Ariana Rostami et al.   Cell Reports

The senescence-associated secretome as an indicator of age and medical risk

(2020) Marissa J. Schafer et al.   JCI Insight

Photoacoustic nanoprobe for β-galactosidase activity detection and imaging in vivo

(2019) Qin Zeng et al.   Journal of Innovative Optical Health Sciences

Real-time imaging of senescence in tumors with DNA damage

(2019) Ying Wang et al.   Scientific Reports

Autofluorescence is a Reliable in vitro Marker of Cellular Senescence in Human Mesenchymal Stromal Cells

(2019) Alessandro Bertolo et al.   Scientific Reports

Cells exhibiting strong p16INK4a promoter activation in vivo display features of senescence

(2019) Jie-Yu Liu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Length‐independent telomere damage drives post‐mitotic cardiomyocyte senescence

(2019) Rhys Anderson et al.   EMBO JOURNAL

Targeted Elimination of Senescent Beta Cells Prevents Type 1 Diabetes

(2019) Peter J. Thompson et al.   Cell Metabolism

Excessive Cell Growth Causes Cytoplasm Dilution And Contributes to Senescence

(2019) Gabriel E. Neurohr et al.   CELL

Extranuclear DNA accumulates in aged cells and contributes to senescence and inflammation

(2019) Yuk Yuen Lan et al.   AGING CELL

Identification of stable senescence-associated reference genes

(2019) Alejandra Hernandez-Segura et al.   AGING CELL

Raman fingerprints as promising markers of cellular senescence and aging

(2019) Lisa Liendl et al.   GeroScience

Replicative and radiation-induced aging: a comparison of gene expression profiles

(2019) Alexander M. Aliper et al.   Aging-US

Exosomes as Emerging Pro-Tumorigenic Mediators of the Senescence-Associated Secretory Phenotype

(2019) Rekha Jakhar et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Senolytic therapy alleviates Aβ-associated oligodendrocyte progenitor cell senescence and cognitive deficits in an Alzheimer’s disease model

(2019) Peisu Zhang et al.   NATURE NEUROSCIENCE

Rapid Detection of Senescent Mesenchymal Stromal Cells by a Fluorescent Probe

(2019) Joshur Ang et al.   Biotechnology Journal

Acceleration of β Cell Aging Determines Diabetes and Senolysis Improves Disease Outcomes

(2019) Cristina Aguayo-Mazzucato et al.   Cell Metabolism

Regulation of Survival Networks in Senescent Cells: From Mechanisms to Interventions

(2019) Abel Soto-Gamez et al.   JOURNAL OF MOLECULAR BIOLOGY

Transcriptome signature of cellular senescence

(2019) Gabriel Casella et al.   NUCLEIC ACIDS RESEARCH

Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3

(2019) Michela Borghesan et al.   Cell Reports

Mitochondrial Homeostasis and Cellular Senescence

(2019) Panagiotis V.S. Vasileiou et al.   Cells

Cellular Senescence: Defining a Path Forward

(2019) Vassilis Gorgoulis et al.   CELL

Targeting senescent cells in translational medicine

(2019) Marta Paez‐Ribes et al.   EMBO Molecular Medicine

A dynamical systems model for the measurement of cellular senescence

(2019) Daniel Galvis et al.   Journal of the Royal Society Interface

Typhoid toxin exhausts the RPA response to DNA replication stress driving senescence and Salmonella infection

(2019) Angela E. M. Ibler et al.   Nature Communications

Identification of reference genes for RT-qPCR data normalisation in aging studies

(2019) Lourdes González-Bermúdez et al.   Scientific Reports

Diversity of the Senescence Phenotype of Cancer Cells Treated with Chemotherapeutic Agents

(2019) Agnieszka Bojko et al.   Cells

Astrocyte senescence may drive alterations in GFAPα, CDKN2A p14ARF, and TAU3 transcript expression and contribute to cognitive decline

(2019) Jed J. Lye et al.   GeroScience

Cellular senescence and chronological age in various human tissues: A systematic review and meta‐analysis

(2019) Camilla S. L. Tuttle et al.   AGING CELL

Increased Iron Deposition on Brain Quantitative Susceptibility Mapping Correlates with Decreased Cognitive Function in Alzheimer’s Disease

(2018) Lei Du et al.   ACS Chemical Neuroscience

Development of highly sensitive fluorescent probes for the detection of β-galactosidase activity – application to the real-time monitoring of senescence in live cells

(2018) Mauro Safir Filho et al.   ANALYST

Paracrine roles of cellular senescence in promoting tumourigenesis

(2018) Jose Mario Gonzalez-Meljem et al.   BRITISH JOURNAL OF CANCER

Selective elimination of senescent cells by mitochondrial targeting is regulated by ANT2

(2018) Sona Hubackova et al.   CELL DEATH AND DIFFERENTIATION

A versatile drug delivery system targeting senescent cells

(2018) Daniel Muñoz‐Espín et al.   EMBO Molecular Medicine

SCAMP4 enhances the senescent cell secretome

(2018) Kyoung Mi Kim et al.   GENES & DEVELOPMENT

Downregulation of cytoplasmic DNases is implicated in cytoplasmic DNA accumulation and SASP in senescent cells

(2018) Akiko Takahashi et al.   Nature Communications

Iron accumulation in senescent cells is coupled with impaired ferritinophagy and inhibition of ferroptosis

(2018) Shashank Masaldan et al.   Redox Biology

Qualitative and quantitative alterations in intracellular and membrane glycoproteins maintain the balance between cellular senescence and human aging

(2018) Yoko Itakura et al.   Aging-US

Clearance of senescent glial cells prevents tau-dependent pathology and cognitive decline

(2018) Tyler J. Bussian et al.   NATURE

Pan-senescence transcriptome analysis identified RRAD as a marker and negative regulator of cellular senescence

(2018) Zhao Wei et al.   FREE RADICAL BIOLOGY AND MEDICINE

Targeted Mitochondrial COQ10 Delivery Attenuates Antiretroviral-Drug-Induced Senescence of Neural Progenitor Cells

(2018) Martina Velichkovska et al.   MOLECULAR PHARMACEUTICS

The dynamic nature of senescence in cancer

(2018) Soyoung Lee et al.   NATURE CELL BIOLOGY

Raman and infrared spectroscopy reveal that proliferating and quiescent human fibroblast cells age by biochemically similar but not identical processes

(2018) Katharina Eberhardt et al.   PLoS One

Identification of senescent cell surface targetable protein DPP4

(2017) Kyoung Mi Kim et al.   GENES & DEVELOPMENT

An OFF–ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo

(2017) Beatriz Lozano-Torres et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

CD9 monoclonal antibody-conjugated PEGylated liposomes for targeted delivery of rapamycin in the treatment of cellular senescence

(2017) Hanh Thuy Nguyen et al.   NANOTECHNOLOGY

Cytoplasmic chromatin triggers inflammation in senescence and cancer

(2017) Zhixun Dou et al.   NATURE

Local clearance of senescent cells attenuates the development of post-traumatic osteoarthritis and creates a pro-regenerative environment

(2017) Ok Hee Jeon et al.   NATURE MEDICINE

Protein Posttranslational Modifications: Roles in Aging and Age-Related Disease

(2017) Ana L. Santos et al.   Oxidative Medicine and Cellular Longevity

Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody

(2017) David Frescas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

cGAS Conducts Micronuclei DNA Surveillance

(2017) Carina C. de Oliveira Mann et al.   TRENDS IN CELL BIOLOGY

Cellular senescence mediates fibrotic pulmonary disease

(2017) Marissa J. Schafer et al.   Nature Communications

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells

(2017) Akiko Takahashi et al.   Nature Communications

Loss of lamin B1 is a biomarker to quantify cellular senescence in photoaged skin

(2017) Audrey Shimei Wang et al.   Scientific Reports

Decoy TRAIL receptor CD264: a cell surface marker of cellular aging for human bone marrow-derived mesenchymal stem cells

(2017) Sean D. Madsen et al.   Stem Cell Research & Therapy

Telomeres and Cell Senescence - Size Matters Not

(2017) Stella Victorelli et al.   EBioMedicine

Robust, universal biomarker assay to detect senescent cells in biological specimens

(2016) Konstantinos Evangelou et al.   AGING CELL

Detection of LacZ -Positive Cells in Living Tissue with Single-Cell Resolution

(2016) Tomohiro Doura et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A scalable label-free approach to separate human pluripotent cells from differentiated derivatives

(2016) N. A. Willoughby et al.   Biomicrofluidics

Conserved and novel functions of programmed cellular senescence during vertebrate development

(2016) Hongorzul Davaapil et al.   DEVELOPMENT

Mitochondria and senescence: new actors for an old play

(2016) Nicolás Herranz et al.   EMBO JOURNAL

Mitochondria are required for pro‐ageing features of the senescent phenotype

(2016) Clara Correia‐Melo et al.   EMBO JOURNAL

Real-Time Tracking and In Vivo Visualization of β-Galactosidase Activity in Colorectal Tumor with a Ratiometric Near-Infrared Fluorescent Probe

(2016) Kaizhi Gu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

NOTCH1 mediates a switch between two distinct secretomes during senescence

(2016) Matthew Hoare et al.   NATURE CELL BIOLOGY

Senescent intimal foam cells are deleterious at all stages of atherosclerosis

(2016) B. G. Childs et al.   SCIENCE

Directed elimination of senescent cells by inhibition of BCL-W and BCL-XL

(2016) Reut Yosef et al.   Nature Communications

N- and O-glycan cell surface protein modifications associated with cellular senescence and human aging

(2016) Yoko Itakura et al.   Cell and Bioscience

The Cell-Cycle Arrest and Apoptotic Functions of p53 in Tumor Initiation and Progression

(2016) Jiandong Chen   Cold Spring Harbor Perspectives in Medicine

Specific lipofuscin staining as a novel biomarker to detect replicative and stress-induced senescence. A method applicable in cryo-preserved and archival tissues

(2016) EA Georgakopoulou et al.   Aging-US

Vascular Smooth Muscle Cell Senescence Promotes Atherosclerosis and Features of Plaque Vulnerability

(2015) Martin Bennett et al.   CIRCULATION

Cellular senescence in aging and age-related disease: from mechanisms to therapy

(2015) Bennett G Childs et al.   NATURE MEDICINE

Comparative Meta-Analysis of Transcriptomics Data during Cellular Senescence andIn VivoTissue Ageing

(2015) Konstantinos Voutetakis et al.   Oxidative Medicine and Cellular Longevity

Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo

(2015) Daisuke Asanuma et al.   Nature Communications

Mitotic Stress Is an Integral Part of the Oncogene-Induced Senescence Program that Promotes Multinucleation and Cell Cycle Arrest

(2015) Dina Dikovskaya et al.   Cell Reports

Ratiometric Two-Photon Fluorescent Probe for Quantitative Detection of β-Galactosidase Activity in Senescent Cells

(2014) Hyo Won Lee et al.   ANALYTICAL CHEMISTRY

An Essential Role for Senescent Cells in Optimal Wound Healing through Secretion of PDGF-AA

(2014) Marco Demaria et al.   DEVELOPMENTAL CELL

Cellular senescence: from physiology to pathology

(2014) Daniel Muñoz-Espín et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Characterization of novel markers of senescence and their prognostic potential in cancer

(2014) M Althubiti et al.   Cell Death & Disease

Notch3 Functions as a Tumor Suppressor by Controlling Cellular Senescence

(2013) H. Cui et al.   CANCER RESEARCH

Monitoring Tumorigenesis and Senescence In Vivo with a p16INK4a-Luciferase Model

(2013) Christin E. Burd et al.   CELL

Programmed Cell Senescence during Mammalian Embryonic Development

(2013) Daniel Muñoz-Espín et al.   CELL

Senescence Is a Developmental Mechanism that Contributes to Embryonic Growth and Patterning

(2013) Mekayla Storer et al.   CELL

α-Fucosidase as a novel convenient biomarker for cellular senescence

(2013) Dominic Hildebrand et al.   CELL CYCLE

Resveratrol alters the lipid composition, metabolism and peroxide level in senescent rat hepatocytes

(2013) Albena Momchilova et al.   CHEMICO-BIOLOGICAL INTERACTIONS

A senescent cell bystander effect: senescence-induced senescence

(2012) Glyn Nelson et al.   AGING CELL

Dual 19F/1H MR Gene Reporter Molecules for in Vivo Detection of β-Galactosidase

(2012) Jian-Xin Yu et al.   BIOCONJUGATE CHEMISTRY

The profile of lysosomal exoglycosidases in replicative and stress-induced senescence in early passage human fibroblasts

(2012) Małgorzata Knaś et al.   FOLIA HISTOCHEMICA ET CYTOBIOLOGICA

Lamin B1 loss is a senescence-associated biomarker

(2012) Adam Freund et al.   MOLECULAR BIOLOGY OF THE CELL

Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation

(2012) Marzia Fumagalli et al.   NATURE CELL BIOLOGY

Pseudo-DNA damage response in senescent cells

(2011) Tatyana V. Pospelova et al.   CELL CYCLE

Senescence surveillance of pre-malignant hepatocytes limits liver cancer development

(2011) Tae-Won Kang et al.   NATURE

Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders

(2011) Darren J. Baker et al.   NATURE

Interplay between oncogene-induced DNA damage response and heterochromatin in senescence and cancer

(2011) Raffaella Di Micco et al.   NATURE CELL BIOLOGY

The Senescence-Associated Secretory Phenotype: The Dark Side of Tumor Suppression

(2010) Jean-Philippe Coppé et al.   Annual Review of Pathology-Mechanisms of Disease

Connecting autophagy to senescence in pathophysiology

(2010) Andrew RJ Young et al.   CURRENT OPINION IN CELL BIOLOGY

The essence of senescence

(2010) T. Kuilman et al.   GENES & DEVELOPMENT

DNA-SCARS: distinct nuclear structures that sustain damage-induced senescence growth arrest and inflammatory cytokine secretion

(2010) F. Rodier et al.   JOURNAL OF CELL SCIENCE

S-Gal®, A novel 1H MRI reporter for β-galactosidase

(2010) Weina Cui et al.   MAGNETIC RESONANCE IN MEDICINE

The matricellular protein CCN1 induces fibroblast senescence and restricts fibrosis in cutaneous wound healing

(2010) Joon-Il Jun et al.   NATURE CELL BIOLOGY

Protocols to detect senescence-associated beta-galactosidase (SA-βgal) activity, a biomarker of senescent cells in culture and in vivo

(2009) Florence Debacq-Chainiaux et al.   Nature Protocols

Targeting lipophilic cations to mitochondria

(2008) Michael P. Murphy   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Senescence of Activated Stellate Cells Limits Liver Fibrosis

(2008) Valery Krizhanovsky et al.   CELL

Oncogene activation in melanocytes links reactive oxygen to multinucleated phenotype and senescence

(2008) C Leikam et al.   ONCOGENE