Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases

The Soybean Lipoxygenase–Substrate Complex: Correlation between the Properties of Tunneling-Ready States and ENDOR-Detected Structures of Ground States

(2020) Adam R. Offenbacher et al.   BIOCHEMISTRY

Biosynthesis of the Maresin Intermediate, 13S,14S-Epoxy-DHA, by Human 15-Lipoxygenase and 12-Lipoxygenase and Its Regulation through Negative Allosteric Modulators

(2020) Cody Freedman et al.   BIOCHEMISTRY

15-Lipoxygenase-1 biosynthesis of 7S,14S-diHDHA implicates 15-Lipoxygenase-2 in biosynthesis of resolvin D5

(2020) Steven C Perry et al.   JOURNAL OF LIPID RESEARCH

Structural and mechanistic insights into 5-lipoxygenase inhibition by natural products

(2020) Nathaniel C. Gilbert et al.   Nature Chemical Biology

Biophysical Characterization of a Disabled Double Mutant of Soybean Lipoxygenase: The “Undoing” of Precise Substrate Positioning Relative to Metal Cofactor and an Identified Dynamical Network

(2019) Shenshen Hu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Targeting biosynthetic networks of the proinflammatory and proresolving lipid metabolome

(2019) Markus Werner et al.   FASEB JOURNAL

Bioinformatic Mapping of Radical S-Adenosylmethionine-Dependent Ribosomally Synthesized and Post-Translationally Modified Peptides Identifies New Cα, Cβ, and Cγ-Linked Thioether-Containing Peptides

(2019) Graham A. Hudson et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Kinetic Characterization of the C–H Activation Step for the Lipoxygenase from the Pathogenic Fungus Magnaporthe oryzae: Impact of N-Linked Glycosylation

(2019) Anastasiia Kostenko et al.   BIOCHEMISTRY

Oxygenation reactions catalyzed by the F557V mutant of soybean lipoxygenase-1: Evidence for two orientations of substrate binding

(2019) Dillon Hershelman et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Comparative kinetic isotope effects on first- and second-order rate constants of soybean lipoxygenase variants uncover a substrate-binding network

(2019) Shenshen Hu et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Detecting and Characterizing the Kinetic Activation of Thermal Networks in Proteins: Thermal Transfer from a Distal, Solvent-Exposed Loop to the Active Site in Soybean Lipoxygenase

(2019) Jan Paulo T. Zaragoza et al.   JOURNAL OF PHYSICAL CHEMISTRY B

H/D exchange mass spectrometry and statistical coupling analysis reveal a role for allostery in a ferredoxin-dependent bifurcating transhydrogenase catalytic cycle

(2018) Luke Berry et al.   BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS

Calmodulin-induced Conformational Control and Allostery Underlying Neuronal Nitric Oxide Synthase Activation

(2018) Quinlin M. Hanson et al.   JOURNAL OF MOLECULAR BIOLOGY

Fundamental Insights into Proton-Coupled Electron Transfer in Soybean Lipoxygenase from Quantum Mechanical/Molecular Mechanical Free Energy Simulations

(2018) Pengfei Li et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Maresins: Specialized Proresolving Lipid Mediators and Their Potential Role in Inflammatory-Related Diseases

(2018) Shi Tang et al.   MEDIATORS OF INFLAMMATION

Understanding Biological Hydrogen Transfer Through the Lens of Temperature Dependent Kinetic Isotope Effects

(2018) Judith P. Klinman et al.   ACCOUNTS OF CHEMICAL RESEARCH

Probing the Electrostatic and Steric Requirements for Substrate Binding in Human Platelet-Type 12-Lipoxygenase

(2018) Ansari Mukhtar Aleem et al.   BIOCHEMISTRY

Mechanistic elucidation of the mycofactocin-biosynthetic radicalS-adenosylmethionine protein, MftC

(2017) Bulat Khaliullin et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Hydrogen–deuterium exchange reveals long-range dynamical allostery in soybean lipoxygenase

(2017) Adam R. Offenbacher et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The expansive role of oxylipins on platelet biology

(2017) Jennifer Yeung et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Tunneling Kinetics and Nonadiabatic Proton-Coupled Electron Transfer in Proteins: The Effect of Electric Fields and Anharmonic Donor–Acceptor Interactions

(2017) Bridget Salna et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Origins of Enzyme Catalysis: Experimental Findings for C–H Activation, New Models, and Their Relevance to Prevailing Theoretical Constructs

(2017) Judith P. Klinman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

13C ENDOR Spectroscopy of Lipoxygenase–Substrate Complexes Reveals the Structural Basis for C–H Activation by Tunneling

(2017) Masaki Horitani et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Enhanced Rigidification within a Double Mutant of Soybean Lipoxygenase Provides Experimental Support for Vibronically Nonadiabatic Proton-Coupled Electron Transfer Models

(2017) Shenshen Hu et al.   ACS Catalysis

Hydrogen–Deuterium Exchange of Lipoxygenase Uncovers a Relationship between Distal, Solvent Exposed Protein Motions and the Thermal Activation Barrier for Catalytic Proton-Coupled Electron Tunneling

(2017) Adam R. Offenbacher et al.   ACS Central Science

Design of a novel thiophene inhibitor of 15-lipoxygenase-1 with both anti-inflammatory and neuroprotective properties

(2016) Nikolaos Eleftheriadis et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Proton-coupled electron transfer reactions: analytical rate constants and case study of kinetic isotope effects in lipoxygenase

(2016) Alexander V. Soudackov et al.   FARADAY DISCUSSIONS

Control of the Position of Oxygen Delivery in Soybean Lipoxygenase-1 by Amino Acid Side Chains within a Gas Migration Channel

(2016) Lara Collazo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Hydrogen/Deuterium Exchange Kinetics Demonstrate Long Range Allosteric Effects of Thumb Site 2 Inhibitors of Hepatitis C Viral RNA-dependent RNA Polymerase

(2016) Daniel Deredge et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Membrane-dependent Activities of Human 15-LOX-2 and Its Murine Counterpart

(2016) Gunes Bender et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structure of Manganese Lipoxygenase of the Rice Blast FungusMagnaporthe oryzae

(2016) Anneli Wennman et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Demonstration That the RadicalS-Adenosylmethionine (SAM) Enzyme PqqE Catalyzesde NovoCarbon-Carbon Cross-linking within a Peptide Substrate PqqA in the Presence of the Peptide Chaperone PqqD

(2016) Ian Barr et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Beyond ferryl-mediated hydroxylation: 40 years of the rebound mechanism and C–H activation

(2016) Xiongyi Huang et al.   JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY

Crystal structure of linoleate 13R-manganese lipoxygenase in complex with an adhesion protein

(2016) Yang Chen et al.   JOURNAL OF LIPID RESEARCH

Kinetics of Bis-Allylic Hydroperoxide Synthesis in the Iron-Containing Lipoxygenase 2 from Cyanothece and the Effects of Manganese Substitution

(2016) Julia Newie et al.   LIPIDS

Proton-coupled electron transfer reactions: analytical rate constants and case study of kinetic isotope effects in lipoxygenase

(2016) Alexander V. Soudackov et al.   FARADAY DISCUSSIONS

Expression and characterization of manganese lipoxygenase of the rice blast fungus reveals prominent sequential lipoxygenation of α-linolenic acid

(2015) Anneli Wennman et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Identification of the Substrate Access Portal of 5-Lipoxygenase

(2015) Sunayana Mitra et al.   BIOCHEMISTRY

A conserved π–cation and an electrostatic bridge are essential for 11R-lipoxygenase catalysis and structural stability

(2015) Priit Eek et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Mammalian lipoxygenases and their biological relevance

(2015) Hartmut Kuhn et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

3-Substituted pyrazoles and 4-substituted triazoles as inhibitors of human 15-lipoxygenase-1

(2015) Benjamin Pelcman et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Identification of 6-benzyloxysalicylates as a novel class of inhibitors of 15-lipoxygenase-1

(2015) Nikolaos Eleftheriadis et al.   EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY

Maresin 1 Biosynthesis and Proresolving Anti-infective Functions with Human-Localized Aggressive Periodontitis Leukocytes

(2015) Chin-Wei Wang et al.   INFECTION AND IMMUNITY

The RadicalS-Adenosyl-l-methionine Enzyme QhpD Catalyzes Sequential Formation of Intra-protein Sulfur-to-Methylene Carbon Thioether Bonds

(2015) Tadashi Nakai et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure and biosynthesis of a macrocyclic peptide containing an unprecedented lysine-to-tryptophan crosslink

(2015) Kelsey R. Schramma et al.   Nature Chemistry

The structural basis for specificity in lipoxygenase catalysis

(2015) Marcia E. Newcomer et al.   PROTEIN SCIENCE

Connecting Lipoxygenase Function to Structure by Electron Paramagnetic Resonance

(2014) Betty J. Gaffney   ACCOUNTS OF CHEMICAL RESEARCH

A mutation interfering with 5-lipoxygenase domain interaction leads to increased enzyme activity

(2014) Marija Rakonjac Ryge et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Fluctuations of an Exposed π-Helix Involved in Lipoxygenase Substrate Recognition

(2014) Miles D. Bradshaw et al.   BIOCHEMISTRY

Hydrogen Tunneling in a Prokaryotic Lipoxygenase

(2014) Cody A. Marcus Carr et al.   BIOCHEMISTRY

The Structure of Human 15-Lipoxygenase-2 with a Substrate Mimic

(2014) Matthew J. Kobe et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Mechanistic Diversity of RadicalS-Adenosylmethionine (SAM)-dependent Methylation

(2014) Matthew R. Bauerle et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Crystal Structure of a Lipoxygenase in Complex with Substrate

(2014) David B. Neau et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Potent and Selective Inhibitors of Human Reticulocyte 12/15-Lipoxygenase as Anti-Stroke Therapies

(2014) Ganesha Rai et al.   JOURNAL OF MEDICINAL CHEMISTRY

Synthesis and Structure–Activity Relationship Studies of 4-((2-Hydroxy-3-methoxybenzyl)amino)benzenesulfonamide Derivatives as Potent and Selective Inhibitors of 12-Lipoxygenase

(2014) Diane K. Luci et al.   JOURNAL OF MEDICINAL CHEMISTRY

Importance of Protein Dynamics during Enzymatic C–H Bond Cleavage Catalysis

(2013) Judith P. Klinman   BIOCHEMISTRY

Kinetic and Structural Investigations into the Allosteric and pH Effect on the Substrate Specificity of Human Epithelial 15-Lipoxygenase-2

(2013) Netra Joshi et al.   BIOCHEMISTRY

Locating a Lipid at the Portal to the Lipoxygenase Active Site

(2012) Betty J. Gaffney et al.   BIOPHYSICAL JOURNAL

Structure of a Calcium-dependent 11R-Lipoxygenase Suggests a Mechanism for Ca2+Regulation

(2012) Priit Eek et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

The N-terminal β-barrel domain of mammalian lipoxygenases including mouse 5-lipoxygenase is not essential for catalytic activity and membrane binding but exhibits regulatory functions

(2011) Matthias Walther et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Pyrazole-based sulfonamide and sulfamides as potent inhibitors of mammalian 15-lipoxygenase

(2011) Khehyong Ngu et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Resolvins and Protectins in Inflammation Resolution

(2011) Charles N. Serhan et al.   CHEMICAL REVIEWS

The Structure of Human 5-Lipoxygenase

(2011) N. C. Gilbert et al.   SCIENCE

Molecular enzymology of lipoxygenases

(2010) Igor Ivanov et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

A novel role for iron in modulating the activity and membrane-binding ability of a trimmed soybean lipoxygenase-1

(2010) Enrico Dainese et al.   FASEB JOURNAL

Identification of novel families and classification of the C2 domain superfamily elucidate the origin and evolution of membrane targeting activities in eukaryotes

(2010) Dapeng Zhang et al.   GENE

Discovery of Potent and Selective Inhibitors of Human Reticulocyte 15-Lipoxygenase-1

(2010) Ganesha Rai et al.   JOURNAL OF MEDICINAL CHEMISTRY

A new model for the origin of kinetic hydrogen isotope effects

(2010) Judith P. Klinman   JOURNAL OF PHYSICAL ORGANIC CHEMISTRY

Kinetic and Structural Investigations of the Allosteric Site in Human Epithelial 15-Lipoxygenase-2

(2009) Aaron T. Wecksler et al.   BIOCHEMISTRY

Substrate specificity effects of lipoxygenase products and inhibitors on soybean lipoxygenase-1

(2009) Aaron T. Wecksler et al.   BIOORGANIC & MEDICINAL CHEMISTRY

Frontiers in enzymatic C–H-bond activation

(2009) J Martin Bollinger et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Lessons from nature: unraveling biological CH bond activation

(2009) Kari L Stone et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Substrate Specificity Changes for Human Reticulocyte and Epithelial 15-Lipoxygenases Reveal Allosteric Product Regulation†

(2008) Aaron T. Wecksler et al.   BIOCHEMISTRY

Enzyme structure and dynamics affect hydrogen tunneling: The impact of a remote side chain (I553) in soybean lipoxygenase-1

(2008) M. P. Meyer et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA