Dynamic Environmental Control in Microfluidic Single‐Cell Cultivations: From Concepts to Applications

Mechanistic Origin of Cell-Size Control and Homeostasis in Bacteria

(2019) Fangwei Si et al.   CURRENT BIOLOGY

Autonomous microfluidic actuators for periodic sequential flow generation

(2019) Zhenglin Li et al.   Science Advances

Ultra-multiplexed analysis of single-cell dynamics reveals logic rules in differentiation

(2019) Ce Zhang et al.   Science Advances

Metabolic activity affects the response of single cells to a nutrient switch in structured populations

(2019) Alma Dal Co et al.   Journal of the Royal Society Interface

Reproduction of Large-Scale Bioreactor Conditions on Microfluidic Chips

(2019) Phuong Ho et al.   Microorganisms

Microfluidic single-cell analysis in biotechnology: from monitoring towards understanding

(2019) Christian Dusny et al.   CURRENT OPINION IN BIOTECHNOLOGY

Dynamic Generation of Concentration- and Temporal-Dependent Chemical Signals in an Integrated Microfluidic Device for Single-Cell Analysis

(2018) Alan M. Gonzalez-Suarez et al.   ANALYTICAL CHEMISTRY

Microbioreactor Systems for Accelerated Bioprocess Development

(2018) Johannes Hemmerich et al.   Biotechnology Journal

Quantitative measurements in single-cell analysis: towards scalability in microbial bioprocess development

(2018) Philipp Demling et al.   CURRENT OPINION IN BIOTECHNOLOGY

Visualization of tandem repeat mutagenesis in Bacillus subtilis

(2018) Miriam Dormeyer et al.   DNA REPAIR

Quantitative modelling of nutrient-limited growth of bacterial colonies in microfluidic cultivation

(2018) Raphael Hornung et al.   Journal of the Royal Society Interface

Real-time dynamics of mutagenesis reveal the chronology of DNA repair and damage tolerance responses in single cells

(2018) Stephan Uphoff   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mutation dynamics and fitness effects followed in single cells

(2018) Lydia Robert et al.   SCIENCE

Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software

(2018) Matthias Kaiser et al.   Nature Communications

Adaptation to fluctuations in temperature by nine species of bacteria

(2018) Kati Saarinen et al.   Ecology and Evolution

Microbioreactor Systems for Accelerated Bioprocess Development

(2018) Johannes Hemmerich et al.   Biotechnology Journal

Analysis of Factors Limiting Bacterial Growth in PDMS Mother Machine Devices

(2018) Da Yang et al.   Frontiers in Microbiology

From industrial fermentor to CFD-guided downscaling: what have we learned?

(2018) Cees Haringa et al.   BIOCHEMICAL ENGINEERING JOURNAL

Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants

(2018) Jennifer Hage-Hülsmann et al.   PLoS One

Regulation of Gene Expression and Signaling Pathway Activity in Mammalian Cells by Automated Microfluidics Feedback Control

(2018) Lorena Postiglione et al.   ACS Synthetic Biology

Development of a microfluidic device with precise on-chip temperature control by integrated cooling and heating components for single cell-based analysis

(2018) Ji Peng et al.   INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER

Universal signal generator for dynamic cell stimulation

(2017) Andreas Piehler et al.   LAB ON A CHIP

Shaping bacterial population behavior through computer-interfaced control of individual cells

(2017) Remy Chait et al.   Nature Communications

Concentration gradient generation methods based on microfluidic systems

(2017) Xiang Wang et al.   RSC Advances

Optogenetic Regulation of Tunable Gene Expression in Yeast Using Photo-Labile Caged Methionine

(2016) Peter M. Kusen et al.   ACS Chemical Biology

Euler-Lagrange computational fluid dynamics for (bio)reactor scale down: An analysis of organism lifelines

(2016) Cees Haringa et al.   ENGINEERING IN LIFE SCIENCES

Time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion

(2016) Christina E. M. Krämer et al.   Scientific Reports

In-Silico Analysis and Implementation of a Multicellular Feedback Control Strategy in a Synthetic Bacterial Consortium

(2016) Gianfranco Fiore et al.   ACS Synthetic Biology

Rapid inoculation of single bacteria into parallel picoliter fermentation chambers

(2015) Christopher Probst et al.   Analytical Methods

Adaptation in Natural Microbial Populations

(2015) Britt Koskella et al.   Annual Review of Ecology Evolution and Systematics

Spatiotemporal microbial single-cell analysis using a high-throughput microfluidics cultivation platform

(2015) Alexander Grünberger et al.   CYTOMETRY PART A

An automated programmable platform enabling multiplex dynamic stimuli delivery and cellular response monitoring for high-throughput suspension single-cell signaling studies

(2015) Luye He et al.   LAB ON A CHIP

Modeling and CFD simulation of nutrient distribution in picoliter bioreactors for bacterial growth studies on single-cell level

(2015) Christoph Westerwalbesloh et al.   LAB ON A CHIP

Mining the microbial dark matter

(2015) Corie Lok   NATURE

Metabolic co-dependence gives rise to collective oscillations within biofilms

(2015) Jintao Liu et al.   NATURE

High-throughput microfluidics to control and measure signaling dynamics in single yeast cells

(2015) Anders S Hansen et al.   Nature Protocols

Elastomeric microfluidic valve with low, constant opening threshold pressure

(2015) Jaemin Shin et al.   RSC Advances

The Impact of Environmental Fluctuations on Evolutionary Fitness Functions

(2015) Anna Melbinger et al.   Scientific Reports

A Microfluidic Platform for Long-Term Monitoring of Algae in a Dynamic Environment

(2015) Chung Sze Luke et al.   ACS Synthetic Biology

Automatic Control of Gene Expression in Mammalian Cells

(2015) Chiara Fracassi et al.   ACS Synthetic Biology

In Vivo Real-Time Control of Gene Expression: A Comparative Analysis of Feedback Control Strategies in Yeast

(2015) Gianfranco Fiore et al.   ACS Synthetic Biology

Single-cell microfluidics: opportunity for bioprocess development

(2014) Alexander Grünberger et al.   CURRENT OPINION IN BIOTECHNOLOGY

Microfluidic single-cell analysis links boundary environments and individual microbial phenotypes

(2014) Christian Dusny et al.   ENVIRONMENTAL MICROBIOLOGY

Oxygen control with microfluidics

(2014) Martin D. Brennan et al.   LAB ON A CHIP

Application of a Genetically Encoded Biosensor for Live Cell Imaging of L-Valine Production in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains

(2014) Nurije Mustafi et al.   PLoS One

A Microfluidic System for Studying Ageing and Dynamic Single-Cell Responses in Budding Yeast

(2014) Matthew M. Crane et al.   PLoS One

Response of Escherichia coli growth rate to osmotic shock

(2014) E. Rojas et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In-Vivo Real-Time Control of Protein Expression from Endogenous and Synthetic Gene Networks

(2014) Filippo Menolascina et al.   PLoS Computational Biology

Sequentially pulsed fluid delivery to establish soluble gradients within a scalable microfluidic chamber array

(2013) Edward S. Park et al.   Biomicrofluidics

Beyond growth rate 0.6: What drivesCorynebacterium glutamicumto higher growth rates in defined medium

(2013) Simon Unthan et al.   BIOTECHNOLOGY AND BIOENGINEERING

Pumps for microfluidic cell culture

(2013) Chang Kyu Byun et al.   ELECTROPHORESIS

Microfluidic oscillators with widely tunable periods

(2013) Sung-Jin Kim et al.   LAB ON A CHIP

Promoter decoding of transcription factor dynamics involves a trade-off between noise and control of gene expression

(2013) A. S. Hansen et al.   Molecular Systems Biology

Shallow breathing: bacterial life at low O2

(2013) Rachel L. Morris et al.   NATURE REVIEWS MICROBIOLOGY

Taking Control over Control: Use of Product Sensing in Single Cells to Remove Flux Control at Key Enzymes in Biosynthesis Pathways

(2013) Georg Schendzielorz et al.   ACS Synthetic Biology

Polydimethylsiloxane (PDMS) Sub-Micron Traps for Single-Cell Analysis of Bacteria

(2013) Christopher Probst et al.   Micromachines

Picoliter nDEP traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments

(2012) Frederik S. O. Fritzsch et al.   LAB ON A CHIP

The development and application of a single-cell biosensor for the detection of l-methionine and branched-chain amino acids

(2012) Nurije Mustafi et al.   METABOLIC ENGINEERING

Extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms

(2012) Nicole Paczia et al.   Microbial Cell Factories

Precise Spatial and Temporal Control of Oxygen within In Vitro Brain Slices via Microfluidic Gas Channels

(2012) Gerardo Mauleon et al.   PLoS One

Long-term model predictive control of gene expression at the population and single-cell levels

(2012) J. Uhlendorf et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Constant Flow-Driven Microfluidic Oscillator for Different Duty Cycles

(2011) Sung-Jin Kim et al.   ANALYTICAL CHEMISTRY

A robust diffusion-based gradient generator for dynamic cell assays

(2011) Javier Atencia et al.   LAB ON A CHIP

Optical sensors for measuring dynamic changes of cytosolic metabolite levels in yeast

(2011) Clara Bermejo et al.   Nature Protocols

Microfluidic bioreactors for culture of non-adherent cells

(2011) Pranjul Shah et al.   SENSORS AND ACTUATORS B-CHEMICAL

Adaptation of cells to new environments

(2011) Aaron N. Brooks et al.   Wiley Interdisciplinary Reviews-Systems Biology and Medicine

Microvalves and Micropumps for BioMEMS

(2011) Anthony K. Au et al.   Micromachines

Exploring the Low-Pressure Growth Limit: Evolution of Bacillus subtilis in the Laboratory to Enhanced Growth at 5 Kilopascals

(2010) W. L. Nicholson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Robust Growth of Escherichia coli

(2010) Ping Wang et al.   CURRENT BIOLOGY

High-throughput tracking of single yeast cells in a microfluidic imaging matrix

(2010) D. Falconnet et al.   LAB ON A CHIP

The role of physiological heterogeneity in microbial population behavior

(2010) Mary E Lidstrom et al.   Nature Chemical Biology

Streaming Instability in Growing Cell Populations

(2010) William Mather et al.   PHYSICAL REVIEW LETTERS

Culturing Aerobic and Anaerobic Bacteria and Mammalian Cells with a Microfluidic Differential Oxygenator

(2009) Raymond H. W. Lam et al.   ANALYTICAL CHEMISTRY

Metabolic gene regulation in a dynamically changing environment

(2008) Matthew R. Bennett et al.   NATURE

A fast, robust and tunable synthetic gene oscillator

(2008) Jesse Stricker et al.   NATURE

A Microfluidic Device for Temporally Controlled Gene Expression and Long-Term Fluorescent Imaging in Unperturbed Dividing Yeast Cells

(2008) Gilles Charvin et al.   PLoS One

Integrated microfluidic bioprocessor for single-cell gene expression analysis

(2008) N. M. Toriello et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Niche dynamics in space and time

(2008) Peter B. Pearman et al.   TRENDS IN ECOLOGY & EVOLUTION