Continuous directed evolution of proteins with improved soluble expression

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Continuous directed evolution of aminoacyl-tRNA synthetases

(2017) David I Bryson et al.   Nature Chemical Biology

Phage-assisted continuous evolution of proteases with altered substrate specificity

(2017) Michael S. Packer et al.   Nature Communications

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

(2016) K. Nishida et al.   SCIENCE

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

A split intein T7 RNA polymerase for transcriptional AND-logic

(2014) Yolanda Schaerli et al.   NUCLEIC ACIDS RESEARCH

Optimizing recombinant antibodies for intracellular function using hitchhiker-mediated survival selection

(2014) Dujduan Waraho-Zhmayev et al.   PROTEIN ENGINEERING DESIGN & SELECTION

A system for the continuous directed evolution of proteases rapidly reveals drug-resistance mutations

(2014) Bryan C. Dickinson et al.   Nature Communications

Modulating protein stability - directed evolution strategies for improved protein function

(2013) Raymond D. Socha et al.   FEBS Journal

Experimental interrogation of the path dependence and stochasticity of protein evolution using phage-assisted continuous evolution

(2013) B. C. Dickinson et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Library of synthetic transcriptional AND gates built with split T7 RNA polymerase mutants

(2013) D. L. Shis et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Ultrafast Protein Splicing is Common among Cyanobacterial Split Inteins: Implications for Protein Engineering

(2012) Neel H. Shah et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

A potent and highly specific FN3 monobody inhibitor of the Abl SH2 domain

(2010) John Wojcik et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Directed Evolution of Serum Paraoxonase PON3 by Family Shuffling and Ancestor/Consensus Mutagenesis, and Its Biochemical Characterization

(2009) Olga Khersonsky et al.   BIOCHEMISTRY

The naturally splitNpuDnaE intein exhibits an extraordinarily high rate in the proteintrans-splicing reaction

(2009) Joachim Zettler et al.   FEBS LETTERS

Engineering antibody fragments to fold in the absence of disulfide bonds

(2009) Min Jeong Seo et al.   PROTEIN SCIENCE

A Dominant Conformational Role for Amino Acid Diversity in Minimalist Protein–Protein Interfaces

(2008) Ryan N. Gilbreth et al.   JOURNAL OF MOLECULAR BIOLOGY