Life in Phases: Intra- and Inter- Molecular Phase Transitions in Protein Solutions

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Stochasticity of Biological Soft Matter: Emerging Concepts in Intrinsically Disordered Proteins and Biological Phase Separation

(2019) Konstantin K. Turoverov et al.   TRENDS IN BIOCHEMICAL SCIENCES

Molecular interactions underlying liquid−liquid phase separation of the FUS low-complexity domain

(2019) Anastasia C. Murthy et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Neuronal ribonucleoprotein granules: Dynamic sensors of localized signals

(2019) Nadia Formicola et al.   TRAFFIC

Intrinsic Disorder-Based Emergence in Cellular Biology: Physiological and Pathological Liquid-Liquid Phase Transitions in Cells

(2019) April L. Darling et al.   Polymers

Gels of Amyloid Fibers

(2019) Ruizhi Wang et al.   Biomolecules

Liquid–Liquid Phase Separation in Disease

(2019) Simon Alberti et al.   Annual Review of Genetics

Multi-functionality of proteins involved in GPCR and G protein signaling: making sense of structure–function continuum with intrinsic disorder-based proteoforms

(2019) Alexander V. Fonin et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Supramolecular Fuzziness of Intracellular Liquid Droplets: Liquid–Liquid Phase Transitions, Membrane-Less Organelles, and Intrinsic Disorder

(2019) Vladimir N. Uversky   MOLECULES

Coil–Globule Transition Thermodynamics of Poly(N-isopropylacrylamide)

(2019) Maren Podewitz et al.   JOURNAL OF PHYSICAL CHEMISTRY B

In Aqua Veritas: The Indispensable yet Mostly Ignored Role of Water in Phase Separation and Membrane-less Organelles

(2018) Boris Y. Zaslavsky et al.   BIOCHEMISTRY

Functional Implications of Intracellular Phase Transitions

(2018) Alex S. Holehouse et al.   BIOCHEMISTRY

The Molten Globule Concept: 45 Years Later

(2018) V. E. Bychkova et al.   BIOCHEMISTRY-MOSCOW

What is Responsible for Atypical Dependence of the Rate of Amyloid Formation on Protein Concentration: Fibril-Catalyzed Initiation of New Fibrils or Competition with Oligomers?

(2018) Alexei V. Finkelstein et al.   Journal of Physical Chemistry Letters

Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, Interactions, and Phase Separation Altered by Mutation and Arginine Methylation

(2018) Veronica H. Ryan et al.   MOLECULAR CELL

The molecular language of membraneless organelles

(2018) Edward Gomes et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Phase Separation in Biology and Disease

(2018) Julie D. Forman-Kay et al.   JOURNAL OF MOLECULAR BIOLOGY

The wisdom of crowds: regulating cell function through condensed states of living matter

(2017) Simon Alberti   JOURNAL OF CELL SCIENCE

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Some additional remarks to the solution of the protein folding puzzle

(2017) Alexei V. Finkelstein   Physics of Life Reviews

There and back again: Two views on the protein folding puzzle

(2017) Alexei V. Finkelstein et al.   Physics of Life Reviews

Direct observation of structure and dynamics during phase separation of an elastomeric protein

(2017) Sean E. Reichheld et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intrinsically Disordered Proteome of Human Membrane-Less Organelles

(2017) April L. Darling et al.   PROTEOMICS

Liquid phase condensation in cell physiology and disease

(2017) Yongdae Shin et al.   SCIENCE

Cataract-associated P23T γD-crystallin retains a native-like fold in amorphous-looking aggregates formed at physiological pH

(2017) Jennifer C. Boatz et al.   Nature Communications

Liquid–liquid phase separation of the microtubule-binding repeats of the Alzheimer-related protein Tau

(2017) Susmitha Ambadipudi et al.   Nature Communications

Are aberrant phase transitions a driver of cellular aging?

(2016) Simon Alberti et al.   BIOESSAYS

Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism

(2016) Shambaditya Saha et al.   CELL

Liquid–liquid phase separation in cellular signaling systems

(2016) P Andrew Chong et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Droplet organelles?

(2016) Edward M Courchaine et al.   EMBO JOURNAL

p53 Proteoforms and Intrinsic Disorder: An Illustration of the Protein Structure–Function Continuum Concept

(2016) Vladimir Uversky   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Dancing Protein Clouds: The Strange Biology and Chaotic Physics of Intrinsically Disordered Proteins

(2016) Vladimir N. Uversky   JOURNAL OF BIOLOGICAL CHEMISTRY

Physicochemical sequence characteristics that influence S-palmitoylation propensity

(2016) Krishna D. Reddy et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Resolving the ambiguity: Making sense of intrinsic disorder when PDB structures disagree

(2016) Shelly DeForte et al.   PROTEIN SCIENCE

Reduction of the Search Space for the Folding of Proteins at the Level of Formation and Assembly of Secondary Structures: A New View on the Solution of Levinthal′s Paradox

(2015) Alexei V. Finkelstein et al.   CHEMPHYSCHEM

Nuclear bodies: the emerging biophysics of nucleoplasmic phases

(2015) Lian Zhu et al.   CURRENT OPINION IN CELL BIOLOGY

Phase Transition of a Disordered Nuage Protein Generates Environmentally Responsive Membraneless Organelles

(2015) Timothy J. Nott et al.   MOLECULAR CELL

Polymer physics of intracellular phase transitions

(2015) Clifford P. Brangwynne et al.   Nature Physics

Cotranslational protein folding on the ribosome monitored in real time

(2015) W. Holtkamp et al.   SCIENCE

Intrinsically Disordered Proteins and Intrinsically Disordered Protein Regions

(2014) Christopher J. Oldfield et al.   Annual Review of Biochemistry

Liquid-Liquid Phase Separation in Biology

(2014) Anthony A. Hyman et al.   Annual Review of Cell and Developmental Biology

Exceptionally abundant exceptions: comprehensive characterization of intrinsic disorder in all domains of life

(2014) Zhenling Peng et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Conditionally and Transiently Disordered Proteins: Awakening Cryptic Disorder To Regulate Protein Function

(2014) Ursula Jakob et al.   CHEMICAL REVIEWS

Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates

(2014) Vladimir N. Uversky et al.   FEBS LETTERS

Assemblages: Functional units formed by cellular phase separation

(2014) Jeffrey A. Toretsky et al.   JOURNAL OF CELL BIOLOGY

How to Determine the Size of Folding Nuclei of Protofibrils from the Concentration Dependence of the Rate and Lag-Time of Aggregation. I. Modeling the Amyloid Protofibril Formation

(2014) Nikita V. Dovidchenko et al.   JOURNAL OF PHYSICAL CHEMISTRY B

The structural and functional signatures of proteins that undergo multiple events of post-translational modification

(2014) Vikas Pejaver et al.   PROTEIN SCIENCE

Under-folded proteins: Conformational ensembles and their roles in protein folding, function, and pathogenesis

(2013) Vladimir N. Uversky   BIOPOLYMERS

Analysis and consensus of currently available intrinsic protein disorder annotation sources in the MobiDB database

(2013) Tomás Di Domenico et al.   BMC BIOINFORMATICS

Dual Specificity Kinase DYRK3 Couples Stress Granule Condensation/Dissolution to mTORC1 Signaling

(2013) Frank Wippich et al.   CELL

Intrinsic Disorder-based Protein Interactions and their Modulators

(2013) Vladimir N. Uversky   CURRENT PHARMACEUTICAL DESIGN

Stochastic machines as a colocalization mechanism for scaffold protein function

(2013) Bin Xue et al.   FEBS LETTERS

Regulated unfolding of proteins in signaling

(2013) Diana M. Mitrea et al.   FEBS LETTERS

Restrictions to protein folding determined by the protein size

(2013) Alexei V. Finkelstein et al.   FEBS LETTERS

Phase transitions and size scaling of membrane-less organelles

(2013) Clifford P. Brangwynne   JOURNAL OF CELL BIOLOGY

A nuclear F-actin scaffold stabilizes ribonucleoprotein droplets against gravity in large cells

(2013) Marina Feric et al.   NATURE CELL BIOLOGY

Proteoform: a single term describing protein complexity

(2013) Lloyd M Smith et al.   NATURE METHODS

Ordered Disorder of the Astrocytic Dystrophin-Associated Protein Complex in the Norm and Pathology

(2013) Insung Na et al.   PLoS One

Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

(2013) E. Sokolova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Unusual biophysics of intrinsically disordered proteins

(2012) Vladimir N. Uversky   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Molten globules, entropy-driven conformational change and protein folding

(2012) Robert L Baldwin et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A Generic Crystallization-like Model That Describes the Kinetics of Amyloid Fibril Formation

(2012) Rosa Crespo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Orderly order in protein intrinsic disorder distribution: disorder in 3500 proteomes from viruses and the three domains of life

(2012) Bin Xue et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

Disorderness inEscherichia coliproteome: perception of folding fidelity and protein–protein interactions

(2012) Bratati Kahali et al.   JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS

RNA catalysis through compartmentalization

(2012) Christopher A. Strulson et al.   Nature Chemistry

Structural Disorder in Eukaryotes

(2012) Rita Pancsa et al.   PLoS One

Monitoring cotranslational protein folding in mammalian cells at codon resolution

(2012) Y. Han et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Golden triangle for folding rates of globular proteins

(2012) S. O. Garbuzynskiy et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Distinguishing crystal-like amyloid fibrils and glass-like amorphous aggregates from their kinetics of formation

(2012) Y. Yoshimura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploring the binding diversity of intrinsically disordered proteins involved in one-to-many binding

(2012) Wei-Lun Hsu et al.   PROTEIN SCIENCE

Intrinsic disorder in putative protein sequences

(2012) Uros Midic et al.   Proteome Science

Conditional disorder in chaperone action

(2012) James C.A. Bardwell et al.   TRENDS IN BIOCHEMICAL SCIENCES

Intrinsic Disorder in the Human Spliceosomal Proteome

(2012) Iga Korneta et al.   PLoS Computational Biology

Multiparametric Analysis of Intrinsically Disordered Proteins: Looking at Intrinsic Disorder through Compound Eyes

(2011) Vladimir N. Uversky et al.   ANALYTICAL CHEMISTRY

Constructing ensembles for intrinsically disordered proteins

(2011) Charles K Fisher et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Increased structural disorder of proteins encoded on human sex chromosomes

(2011) Hedi Hegyi et al.   Molecular BioSystems

Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes

(2011) C. P. Brangwynne et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Context-dependent resistance to proteolysis of intrinsically disordered proteins

(2011) Marcin J. Suskiewicz et al.   PROTEIN SCIENCE

Expanding the proteome: disordered and alternatively folded proteins

(2011) H. Jane Dyson   QUARTERLY REVIEWS OF BIOPHYSICS

Biogenesis and function of nuclear bodies

(2011) Yuntao S. Mao et al.   TRENDS IN GENETICS

The relationship between proteome size, structural disorder and organism complexity

(2011) Eva Schad et al.   GENOME BIOLOGY

Understanding protein non-folding

(2010) Vladimir N. Uversky et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Archaic chaos: intrinsically disordered proteins in Archaea

(2010) Bin Xue et al.   BMC Systems Biology

Multitude of binding modes attainable by intrinsically disordered proteins: a portrait gallery of disorder-based complexes

(2010) Vladimir N. Uversky   CHEMICAL SOCIETY REVIEWS

Mysterious oligomerization of the amyloidogenic proteins

(2010) Vladimir N. Uversky   FEBS Journal

Power Law Distribution Defines Structural Disorder as a Structural Element Directly Linked with Function

(2010) Peter Tompa et al.   JOURNAL OF MOLECULAR BIOLOGY

Protein Folding as Flow across a Network of Folding−Unfolding Pathways. 2. The “In-Water” Case

(2010) Dmitry N. Ivankov et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Protein Folding as Flow across a Network of Folding−Unfolding Pathways. 1. The Mid-Transition Case

(2010) Dmitry N. Ivankov et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Cotranslational structure acquisition of nascent polypeptides monitored by NMR spectroscopy

(2010) C. Eichmann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The protein kingdom extended: Ordered and intrinsically disordered proteins, their folding, supramolecular complex formation, and aggregation

(2010) Konstantin K. Turoverov et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Conformation of P22 tailspike folding and aggregation intermediates probed by monoclonal antibodies

(2010) Margaret A. Speed et al.   PROTEIN SCIENCE

Structural characterization of the molten globule of α-lactalbumin by solution X-ray scattering

(2010) Mikio Kataoka et al.   PROTEIN SCIENCE

Biogenesis of Nuclear Bodies

(2010) M. Dundr et al.   Cold Spring Harbor Perspectives in Biology

Linking folding and binding

(2009) Peter E Wright et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

The Mysterious Unfoldome: Structureless, Underappreciated, Yet Vital Part of Any Given Proteome

(2009) Vladimir N. Uversky   JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY

Interaction between Intrinsically Disordered Proteins Frequently Occurs in a Human Protein–Protein Interaction Network

(2009) Kana Shimizu et al.   JOURNAL OF MOLECULAR BIOLOGY

In VivoFormation of Protein Based Aqueous Microcompartments

(2009) Xin Ge et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Direct evidence for a dry molten globule intermediate during the unfolding of a small protein

(2009) S. K. Jha et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

How strong are side chain interactions in the folding intermediate?

(2009) Ekaterina N. Samatova et al.   PROTEIN SCIENCE

Modularity of intrinsic disorder in the human proteome

(2009) Melissa M. Pentony et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Identification, analysis, and prediction of protein ubiquitination sites

(2009) Predrag Radivojac et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Germline P Granules Are Liquid Droplets That Localize by Controlled Dissolution/Condensation

(2009) C. P. Brangwynne et al.   SCIENCE

Intrinsically Disordered Proteins in Human Diseases: Introducing the D2 Concept

(2008) Vladimir N. Uversky et al.   Annual Review of Biophysics

Flexible nets: disorder and induced fit in the associations of p53 and 14-3-3 with their partners

(2008) Christopher J Oldfield et al.   BMC GENOMICS

Function and structure of inherently disordered proteins

(2008) A Keith Dunker et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structural features of protein folding nuclei

(2008) S.O. Garbuzynskiy et al.   FEBS LETTERS

Conformational properties of the aggregation precursor state of HypF-N

(2008) Silvia Campioni et al.   JOURNAL OF MOLECULAR BIOLOGY

The Foldon Substructure of Staphylococcal Nuclease

(2008) Sabrina Bédard et al.   JOURNAL OF MOLECULAR BIOLOGY

Intrinsic Disorder in Nuclear Hormone Receptors

(2008) Matthew D. Krasowski et al.   JOURNAL OF PROTEOME RESEARCH

Protein folding: Independent unrelated pathways or predetermined pathway with optional errors

(2008) S. Bedard et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Intrinsic disorder in scaffold proteins: Getting more from less

(2008) Marc S. Cortese et al.   PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

Analysis of solvent content and oligomeric states in protein crystals-does symmetry matter?

(2008) Maksymilian Chruszcz et al.   PROTEIN SCIENCE

BIOCHEMISTRY: Controlled Chaos

(2008) V. N. Uversky et al.   SCIENCE

Tight Regulation of Unstructured Proteins: From Transcript Synthesis to Protein Degradation

(2008) J. Gsponer et al.   SCIENCE

Crystal Structure of a Full-Length β-Catenin

(2008) Yi Xing et al.   STRUCTURE

Do viral proteins possess unique biophysical features?

(2008) Nobuhiko Tokuriki et al.   TRENDS IN BIOCHEMICAL SCIENCES

Intrinsically Disordered Proteins Display No Preference for Chaperone Binding In Vivo

(2008) Hedi Hegyi et al.   PLoS Computational Biology

Protein Phase Behavior in Aqueous Solutions: Crystallization, Liquid-Liquid Phase Separation, Gels, and Aggregates

(2007) André C. Dumetz et al.   BIOPHYSICAL JOURNAL

Structural disorder serves as a weak signal for intracellular protein degradation

(2007) P. Tompa et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Partly folded states of members of the lysozyme/lactalbumin superfamily: A comparative study by circular dichroism spectroscopy and limited proteolysis

(2002) Patrizia Polverino de Laureto et al.   PROTEIN SCIENCE