N-Glycolylneuraminic Acid (Neu5Gc) Null Large Animals by Targeting the CMP-Neu5Gc Hydroxylase (CMAH)

In Search of the Ideal Valve: Optimizing Genetic Modifications to Prevent Bioprosthetic Degeneration

(2019) Benjamin Smood et al.   ANNALS OF THORACIC SURGERY

Biomimetic Glyconanoparticle Vaccine for Cancer Immunotherapy

(2019) Eliran Moshe Reuven et al.   ACS Nano

Generation of cattle knockout for galactose‐α1,3‐galactose and N‐glycolylneuraminic acid antigens

(2019) Andrea Perota et al.   XENOTRANSPLANTATION

Elicited and pre‐existing anti‐Neu5Gc antibodies differentially affect human endothelial cells transcriptome

(2019) Ludmilla Le Berre et al.   XENOTRANSPLANTATION

Absence of Neu5Gc and Presence of Anti-Neu5Gc Antibodies in Humans—An Evolutionary Perspective

(2019) Meghan O. Altman et al.   Frontiers in Immunology

Reducing immunoreactivity of porcine bioprosthetic heart valves by genetically-deleting three major glycan antigens, GGTA1/β4GalNT2/CMAH

(2018) Runjie Zhang et al.   Acta Biomaterialia

Skin xenotransplantation: Historical review and clinical potential

(2018) Takayuki Yamamoto et al.   BURNS

Exposure to nonmicrobial N-glycolylneuraminic acid protects farmers' children against airway inflammation and colitis

(2018) Remo Frei et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Polyclonal human antibodies against glycans bearing red meat-derived non-human sialic acid N-glycolylneuraminic acid are stable, reproducible, complex and vary between individuals: Total antibody levels are associated with colorectal cancer risk

(2018) Annie N. Samraj et al.   PLoS One

Evolution of sialic acids: Implications in xenotransplant biology

(2018) Anu Paul et al.   XENOTRANSPLANTATION

Evolution in primates by “Catastrophic-selection” interplay between enveloped virus epidemics, mutated genes of enzymes synthesizing carbohydrate antigens, and natural anti-carbohydrate antibodies

(2018) Uri Galili   AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY

Phylogenetic Distribution of CMP-Neu5Ac Hydroxylase (CMAH), the Enzyme Synthetizing the Proinflammatory Human Xenoantigen Neu5Gc

(2017) Sateesh Peri et al.   Genome Biology and Evolution

Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9

(2017) Dong Niu et al.   SCIENCE

Anti-Gal and Anti-Neu5Gc Responses in Nonimmunosuppressed Patients After Treatment With Rabbit Antithymocyte Polyclonal IgGs

(2017) Apolline Salama et al.   TRANSPLANTATION

Glycan microarray reveal induced IgGs repertoire shift against a dietary carbohydrate in response to rabbit anti-human thymocyte therapy

(2017) Ron Amon et al.   Oncotarget

Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing

(2016) Ciaran M Lee et al.   MOLECULAR THERAPY

Gene targeting, genome editing: from Dolly to editors

(2016) Wenfang Tan et al.   TRANSGENIC RESEARCH

Characterization of immunogenic Neu5Gc in bioprosthetic heart valves

(2016) Eliran Moshe Reuven et al.   XENOTRANSPLANTATION

Anti-EBOV GP IgGs Lacking α1-3-Galactose and Neu5Gc Prolong Survival and Decrease Blood Viral Load in EBOV-Infected Guinea Pigs

(2016) Olivier Reynard et al.   PLoS One

Mammalian interspecies substitution of immune modulatory alleles by genome editing

(2016) Simon G. Lillico et al.   Scientific Reports

The alpha-gal story: Lessons learned from connecting the dots

(2015) John W. Steinke et al.   JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY

Rabbit antithymocyte globulin–induced serum sickness disease and human kidney graft survival

(2015) Grégoire Couvrat-Desvergnes et al.   JOURNAL OF CLINICAL INVESTIGATION

Therapeutic genome editing: prospects and challenges

(2015) David Benjamin Turitz Cox et al.   NATURE MEDICINE

Evaluation of human and non-human primate antibody binding to pig cells lacking GGTA1/CMAH/β4GalNT2 genes

(2015) Jose L. Estrada et al.   XENOTRANSPLANTATION

Xenograft bioprosthetic heart valves: Past, present and future

(2015) Rizwan A. Manji et al.   International Journal of Surgery

Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs

(2015) Xianlong Wang et al.   Scientific Reports

Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA

(2015) Yong Wang et al.   Scientific Reports

CMP-Neu5Ac Hydroxylase Null Mice as a Model for Studying Metabolic Disorders Caused by the Evolutionary Loss of Neu5Gc in Humans

(2015) Deug-Nam Kwon et al.   Biomed Research International

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Species-Specific Chromosome Engineering Greatly Improves Fully Human Polyclonal Antibody Production Profile in Cattle

(2015) Hiroaki Matsushita et al.   PLoS One

Genome Engineering with Targetable Nucleases

(2014) Dana Carroll   Annual Review of Biochemistry

CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites

(2014) Yuki Naito et al.   BIOINFORMATICS

Parallel evolution of a self-signal: humans and new world monkeys independently lost the cell surface sugar Neu5Gc

(2014) Stevan A. Springer et al.   IMMUNOGENETICS

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Efficient generation of genetically distinct pigs in a single pregnancy using multiplexed single-guide RNA and carbohydrate selection

(2014) Ping Li et al.   XENOTRANSPLANTATION

Potential deleterious role of anti-Neu5Gc antibodies in xenotransplantation

(2014) Apolline Salama et al.   XENOTRANSPLANTATION

Erythrocytes from GGTA1/CMAH knockout pigs: implications for xenotransfusion and testing in non-human primates

(2014) Zheng-Yu Wang et al.   XENOTRANSPLANTATION

Ferrets exclusively synthesize Neu5Ac and express naturally humanized influenza A virus receptors

(2014) Preston S.K. Ng et al.   Nature Communications

Long-Term IgG Response to Porcine Neu5Gc Antigens without Transmission of PERV in Burn Patients Treated with Porcine Skin Xenografts

(2013) L. Scobie et al.   JOURNAL OF IMMUNOLOGY

Physiological Level Production of Antigen-Specific Human Immunoglobulin in Cloned Transchromosomic Cattle

(2013) Akiko Sano et al.   PLoS One

Double knockout pigs deficient in N-glycolylneuraminic acid and Galactose α-1,3-Galactose reduce the humoral barrier to xenotransplantation

(2013) Andrew J. Lutz et al.   XENOTRANSPLANTATION

First quantification of alpha-Gal epitope in current glutaraldehyde-fixed heart valve bioprostheses

(2013) Filippo Naso et al.   XENOTRANSPLANTATION

Production of biallelic CMP-Neu5Ac hydroxylase knock-out pigs

(2013) Deug-Nam Kwon et al.   Scientific Reports

Pancreatic β-cell failure in obese mice with human-like CMP-Neu5Ac hydroxylase deficiency

(2011) Sarah Kavaler et al.   FASEB JOURNAL

Efficient generation of a biallelic knockout in pigs using zinc-finger nucleases

(2011) J. Hauschild et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Sexual selection by female immunity against paternal antigens can fix loss of function alleles

(2011) D. Ghaderi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Novel mechanism for the generation of human xeno-autoantibodies against the nonhuman sialic acidN-glycolylneuraminic acid

(2010) Rachel E. Taylor et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Immunological Property of Antibodies against N-Glycolylneuraminic Acid Epitopes in Cytidine Monophospho-N-Acetylneuraminic Acid Hydroxylase-Deficient Mice

(2010) H. Tahara et al.   JOURNAL OF IMMUNOLOGY

Cardiac xenotransplantation technology provides materials for improved bioprosthetic heart valves

(2010) Christopher G.A. McGregor et al.   JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY

Implications of the presence of N-glycolylneuraminic acid in recombinant therapeutic glycoproteins

(2010) Darius Ghaderi et al.   NATURE BIOTECHNOLOGY

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS

Gal knockout pig pericardium: New source of material for heart valve bioprostheses

(2009) Nermine Lila et al.   JOURNAL OF HEART AND LUNG TRANSPLANTATION

Antigen-specific human polyclonal antibodies from hyperimmunized cattle

(2009) Yoshimi Kuroiwa et al.   NATURE BIOTECHNOLOGY