Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis

CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis

(2019) Zhenghui Lu et al.   NUCLEIC ACIDS RESEARCH

Integrated whole-genome and transcriptome sequence analysis reveals the genetic characteristics of a riboflavin-overproducing Bacillus subtilis

(2018) Guanglu Wang et al.   METABOLIC ENGINEERING

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

(2018) Zehua Bao et al.   NATURE BIOTECHNOLOGY

Construction, Model-Based Analysis, and Characterization of a Promoter Library for Fine-Tuned Gene Expression in Bacillus subtilis

(2018) Dingyu Liu et al.   ACS Synthetic Biology

Development of an Efficient Genome Editing Tool in Bacillus licheniformis Using CRISPR-Cas9 Nickase

(2018) Kaifeng Li et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis

(2018) Kun-Qiang Hong et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway

(2017) Xinna Zhu et al.   METABOLIC ENGINEERING

CRISPR/Cas9-coupled recombineering for metabolic engineering of Corynebacterium glutamicum

(2017) Jae Sung Cho et al.   METABOLIC ENGINEERING

Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

(2017) Francesca Zerbini et al.   Microbial Cell Factories

A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

(2017) Younju So et al.   Frontiers in Microbiology

Precise genome-wide base editing by the CRISPR Nickase system in yeast

(2017) Atsushi Satomura et al.   Scientific Reports

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Development of a CRISPR-Cas9 Tool Kit for Comprehensive Engineering of Bacillus subtilis

(2016) Adam W. Westbrook et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Editing of the Bacillus subtilis Genome by the CRISPR-Cas9 System

(2016) Josef Altenbuchner   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

CRISPR technologies for bacterial systems: Current achievements and future directions

(2016) Kyeong Rok Choi et al.   BIOTECHNOLOGY ADVANCES

CRISPR-based genome editing and expression control systems inClostridium acetobutylicumandClostridium beijerinckii

(2016) Qi Li et al.   Biotechnology Journal

CRISPR/Cas9 advances engineering of microbial cell factories

(2016) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering

(2016) Andrew D Garst et al.   NATURE BIOTECHNOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

CRISPR-based genome editing and expression control systems inClostridium acetobutylicumandClostridium beijerinckii

(2016) Qi Li et al.   Biotechnology Journal

Multigene disruption in undomesticated Bacillus subtilis ATCC 6051a using the CRISPR/Cas9 system

(2016) Kang Zhang et al.   Scientific Reports

Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

(2015) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Bacterial CRISPR: accomplishments and prospects

(2015) Jason M Peters et al.   CURRENT OPINION IN MICROBIOLOGY

Metabolic engineering of Escherichia coli using CRISPR–Cas9 meditated genome editing

(2015) Yifan Li et al.   METABOLIC ENGINEERING

Genome engineering using a synthetic gene circuit in Bacillus subtilis

(2015) Da-Eun Jeong et al.   NUCLEIC ACIDS RESEARCH

A condition-specific codon optimization approach for improved heterologous gene expression in Saccharomyces cerevisiae

(2014) Amanda M Lanza et al.   BMC Systems Biology

Semi-synthetic artemisinin: a model for the use of synthetic biology in pharmaceutical development

(2014) Chris J. Paddon et al.   NATURE REVIEWS MICROBIOLOGY

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Expression-level optimization of a multi-enzyme pathway in the absence of a high-throughput assay

(2013) Michael E. Lee et al.   NUCLEIC ACIDS RESEARCH

Establishment of a Markerless Mutation Delivery System in Bacillus subtilis Stimulated by a Double-Strand Break in the Chromosome

(2013) Ting Shi et al.   PLoS One

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Implementation of stable and complex biological systems through recombinase-assisted genome engineering

(2013) Christine Nicole S. Santos et al.   Nature Communications

FairyTALE: A High-Throughput TAL Effector Synthesis Platform

(2013) Jing Liang et al.   ACS Synthetic Biology

Yeast Oligo-Mediated Genome Engineering (YOGE)

(2013) James E. DiCarlo et al.   ACS Synthetic Biology

The future of metabolic engineering and synthetic biology: Towards a systematic practice

(2012) Vikramaditya G. Yadav et al.   METABOLIC ENGINEERING

Bacillus subtilis genome editing using ssDNA with short homology regions

(2012) Yang Wang et al.   NUCLEIC ACIDS RESEARCH

Impact of two DNA repair pathways, homologous recombination and non-homologous end joining, on bacterial spore inactivation under simulated martian environmental conditions

(2011) Ralf Moeller et al.   ICARUS

Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries

(2011) Jiayuan Quan et al.   Nature Protocols

DNA Nicks Promote Efficient and Safe Targeted Gene Correction

(2011) Luther Davis et al.   PLoS One

A new simple method to introduce marker-free deletions in the Bacillus subtilis genome

(2010) Takuya Morimoto et al.   GENES & GENETIC SYSTEMS

Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides

(2010) Joseph R Warner et al.   NATURE BIOTECHNOLOGY

Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template

(2010) Michael J. Metzger et al.   NUCLEIC ACIDS RESEARCH

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY