CRISPR-based genome editing and expression control systems inClostridium acetobutylicumandClostridium beijerinckii

Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824

(2016) Muhammad Ehsaan et al.   Biotechnology for Biofuels

One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces

(2015) He Huang et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase

(2015) Tao Xu et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Current status and prospects of industrial bio-production of n-butanol in China

(2015) Yu Jiang et al.   BIOTECHNOLOGY ADVANCES

Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system

(2015) Yi Wang et al.   JOURNAL OF BIOTECHNOLOGY

I-SceI-mediated scarless gene modification via allelic exchange in Clostridium

(2015) Ning Zhang et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Targeted Large-Scale Deletion of Bacterial Genomes Using CRISPR-Nickases

(2015) Kylie Standage-Beier et al.   ACS Synthetic Biology

Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system

(2015) Rui Liu et al.   Cell Discovery

Utilization of economical substrate-derived carbohydrates by solventogenic clostridia: pathway dissection, regulation and engineering

(2014) Yang Gu et al.   CURRENT OPINION IN BIOTECHNOLOGY

Metabolic engineering of carbon and redox flow in the production of small organic acids

(2014) Chandresh Thakker et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

High-Efficiency Multiplex Genome Editing of Streptomyces Species Using an Engineered CRISPR/Cas System

(2014) Ryan E. Cobb et al.   ACS Synthetic Biology

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases

(2013) S. W. Cho et al.   GENOME RESEARCH

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

RNA-guided gene activation by CRISPR-Cas9–based transcription factors

(2013) Pablo Perez-Pinera et al.   NATURE METHODS

CRISPR RNA–guided activation of endogenous human genes

(2013) Morgan L Maeder et al.   NATURE METHODS

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration

(2012) Mohab A. Al-Hinai et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Pleiotropic functions of catabolite control protein CcpA in Butanol-producing Clostridium acetobutylicum

(2012) Cong Ren et al.   BMC GENOMICS

Metabolic engineering of d-xylose pathway in Clostridium beijerinckii to optimize solvent production from xylose mother liquid

(2012) Han Xiao et al.   METABOLIC ENGINEERING

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

DNA Nicks Promote Efficient and Safe Targeted Gene Correction

(2011) Luther Davis et al.   PLoS One

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

(2011) B. Wiedenheft et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Development of pyrF-Based Genetic System for Targeted Gene Deletion in Clostridium thermocellum and Creation of a pta Mutant

(2010) S. A. Tripathi et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Single-strand nicks induce homologous recombination with less toxicity than double-strand breaks using an AAV vector template

(2010) Michael J. Metzger et al.   NUCLEIC ACIDS RESEARCH

Engineering Clostridium Strain to Accept Unmethylated DNA

(2010) Hongjun Dong et al.   PLoS One

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

A modular system for Clostridium shuttle plasmids

(2009) John T. Heap et al.   JOURNAL OF MICROBIOLOGICAL METHODS

The ClosTron: Mutagenesis in Clostridium refined and streamlined

(2009) John T. Heap et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Fermentative Butanol Production

(2008) Peter Dürre   Annals of the New York Academy of Sciences

Fermentative butanol production by clostridia

(2008) Sang Yup Lee et al.   BIOTECHNOLOGY AND BIOENGINEERING

PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum

(2008) Falk Hillmann et al.   MOLECULAR MICROBIOLOGY